structure of the review

... amide groups of Ile128 and Arg129. Despite these differences in the coordination of the res- idues in the tip, the fold of the loop is strikingly simi- lar. The rmsd for the Ca atoms of the residues ... conserved. The LL residues of the LLR sequence are important for the stability of the hydrophobic core below the ade- nine group. Fig. 8. A stereoview of the conformation shift of the KMSKS loop. In the ... inhibition of the enzyme. The pos- sibilities for design of a competitive inhibitor are also discussed. These structures exhibit a new structural feature of the CP domain. In one of the complexes, the

Ngày tải lên: 06/03/2014, 22:21

... regrowth upon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generates new neurons throughout the lifespan of the fish [11] The cloning of the RICH proteins ... structure of the RICH catalytic domain The width of the sausage is reversely proportional to the heteronuclear NOE values The figure was gen-erated with MOLMOL [46]. Fig 4 NMR titration of the ... orientation of 3¢-AMP when bound to the catalytic domain of RICH (F) Mapping of the chemical shift changes due to the adenine group of 3¢-AMP on the RICH structure. Fig 6 Catalytic site of RICH Histidines

Ngày tải lên: 07/03/2014, 10:20

... high accuracy, shows clearly the influence of cryo-cooling, which is manifested in shrinkage of the mole-cule and lowering the volume of the unit cell In the structure of the com-plex, two acarbose ... and discussion Description of the structures There is one molecule in the asymmetric unit of both structures composed of a single domain consisting of 14 helices, 12 of them forming an (a⁄ a6) barrel ... native structure [16] The active site is at the narrower end of the barrel as mapped by the presence of ligands (Tris in Glu1.1 or acarbose in the Glu-A structure) Accuracy of models As expected, the

Ngày tải lên: 07/03/2014, 12:20

... deviations of the chemical shifts of the amide nitrogen and proton nuclei Results Determination of the three-dimensional structure of HPr(I14A) To allow the comparison of the structures of HPr(I14A) ... towards the interior of the protein The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig 3 Structure ensemble of HPr(I14A) The average structure of the ... back onto the core of the protein thereby allowing the side chain of Leu86 to partly fill the hole created by the removal of Ile14 Due to these changes other alterations are induced in the HPr(I14A)

Ngày tải lên: 07/03/2014, 16:20

... C of the other subunit In contrast, the TVA-II group dimer attaches the B domain to an N-terminal domain of the other subunit, which restricts the size of the B domain (Fig 7) Active center The ... from the rather arbitrary placing of the gaps Therefore, we took account of the geometry and first derived the group of structurally equivalent residues in a chain-fold superposition and then ... the loops at the opposite barrel end The lengths of the b-strands in the barrel vary from two (b14) to seven (b11) residues As in other enzymes of family no 13, the regularity of the CDase TIM

Ngày tải lên: 08/03/2014, 02:20

... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... 42–44) The secondary structure of RIIa D/D was determined from the coordinates of the ensemble of 24 structures using the program MOLMOL [27] For both helices I (I¢) and II (II¢) the beginning of the ... shows the van der Waals model of the top face of the best structure of RIIa D/D in the region )1 to 44 This view is generated by rotating the orientation of RIIa D/D of Fig 4A by 90° about the

Ngày tải lên: 08/03/2014, 22:20

... enzy-matic part, and (b) the variability of the angle of approach of the inhibitor relative to the catalytic cleft of the enzyme The latter factor may reflect not so much the geometry of the catalytic site ... chagasin–cathepsin L complex (Table 3) The dif-ference of 9 between these complexes may be corre-lated with the variation of the rate of binding and affinity for chagasin of these enzymes On the other ... despite the lack of overall sequence similarity The role of the proline residue appears to be to maintain the specific shape of the loop The aromatic residue, on the other hand, interacts with the

Ngày tải lên: 16/03/2014, 04:20

... mm of monomers at 30C Most of the1H-15N HSQC signals of the dimeric form of PDI-bb¢ coincide with the signals of the monomeric form or are weak as a result of the high molecular weight of the ... struc-ture, the catalytic cysteines face each other; in the other, the catalytic residues of the a domain face away from the a¢ domain The crystal structures also revealed the presence of a hydrophobic ... Superimposition of these structures is shown in Fig 3C The differences could result from (a) an effect of the second b domain in our PDI-bb¢ structure, (b) the I289A mutation, or (c) the presence of the

Ngày tải lên: 16/03/2014, 04:20

... from the core of the structure The solution structure of DnaG-C identifies the crystal Fig 2 Sequence alignment of DnaG-C with homologs from different bacterial species The sequence numbering of ... analysis of linker residues and of residues in the C-terminal domain of B stearothermo-philus DnaB confirmed the importance of residues in these parts of the protein [3,16] Unlike in the wild-type ... half of them The present structure of monomeric E coli DnaG-C identifies the earlier crystal structure of the same pro-tein as a domain-swapped dimer, in which helix 5 of one monomer binds to the

Ngày tải lên: 16/03/2014, 12:20

... that the v:w unit is linked to the single-stranded DNA-binding protein via the distal surface of v The base of the clamp-loader complex has an open C-shaped structure, and the shape of the v:w ... but, like the d and d¢ subunits of the clamp-loader, neither v nor w contain any of the functional elements required for nucleotide binding The topology of the w subunit resembles that of the bacterial ... to the c subunit and the b clamp As expected, the c subunit shows a high degree of conservation in the first two domains that make up the AAA+ATPase portion of the subunit The third domain of the

Ngày tải lên: 16/03/2014, 16:20

... site on the extracellular domain The second step will then be the deposition of the ligand into the TM pocket causing the conformation change of the receptor and triggering the coupling of the receptor ... monitoring the conformation movement and defining the final conformation of the eLPs These studies were limited to the three eLP domains The conformation of the eLPs in the simulated reduced form of the ... [1] Mutation of the conserved sequence WCF in eLP2of the EP receptor has been reported to change the ligand binding recognition of the receptor [30] The relationship of the changes of the conserved

Ngày tải lên: 16/03/2014, 18:20

... shield the active site pocket by occupying the position corresponding to the lid-loop To better understand the significance of the absence of the lid-loop sequence and the presence of the extra ... in most other GGTs at the C-terminal region of the L-subunit In E coli GGT, upon autocatalytic cleavage of the peptide bond on the N-terminal side of Thr391, the C-terminal segment of the newly ... between the two enzymes can be seen in the manner of Fig 3 Comparison of the structures of GGTs from (A) B subtilis, (B) E coli and (C) H pylori The structure of each GGT is indicated in the upper

Ngày tải lên: 22/03/2014, 21:20

... the relevant provisions of the Financial Regulations and Rules of the United Nations examine, review and appraise the use of financial resources of the United Nations in order to guarantee the ... recommending the inclusion of a reference to the work of the United Nations in disaster risk reduction within the sustainable development programme of the United Nations 17 The Management of the ISDR ... Secretary-General (SRSG) for the implementation of the HFA 6 The mandate of the secretariat is to act as the focal point in the United Nations system for the coordination of disaster reduction and

Ngày tải lên: 23/03/2014, 02:20

... understanding of the effect of NPs on the structure of proteins is still in a nascent state In this work we report the unfolding behavior of the periplasmic domain of the ToxR protein (ToxRp) of Vibrio ... effect of ZnO NPs on the structure of ToxRp, alone and in the presence of denaturing agents, and determined the thermodynamic parameters of binding ToxRp is the 96-residue C-terminal domain of the ... by the absorption of H+ from themedium, and the ensuing electrostatic interaction would lead to a degradation of the native structure Because of the lack of homology with any known structure, the

Ngày tải lên: 23/03/2014, 03:20

... in the center of the domain with the active site of the enzyme on one side and the Rieske center on the other side of the sheet (Fig 3) Covering one side of the sheet are two Fig 2 Crystal structure ... following the sequence.) Together with the extended loop, which extends 20 A˚ from the center of the funnel, they form the base of the b subunit (Fig 3) The last four resi-dues in the C-terminal ... for each of the three a subunits LI, on the other hand, could only be partially modeled for one of the three a subunits, the high flexibility of the loop precluded modeling for the two other chains

Ngày tải lên: 23/03/2014, 09:21

... the primary structure of the N-terminal regions of TTR and the biological function of TTR Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during ... other, the N-terminal region of crocTTR was deleted The affinity of T3 and T4 to both TTRs was studied and the results are a powerful demonstration of the rela-tionship between the evolution of the ... that the N-terminal regions of TTR affect the affinities of TTR for thyroid hormones A previous study revealed that altering the structure of the N-ter-minal region influenced the affinity of thyroid

Ngày tải lên: 23/03/2014, 10:21

... result of the anisotropy of the carboxyl carbon of KdoIII The data obtained suggested that OSNaOH-II has the structure shown in Fig 2 The structures of the alkaline degradation products were further ... from the CSD MS data of OSHOAc(see above) These data defined the structure of the OSHOAc(Fig 2) as well as of the full core oligosaccharide of P syringae pv phaseolicola GSPB 711 (Fig 5) The structure ... degaradation of the LPS [21] were used to determine the structure of the core-lipid A carbohydrate backbone of the P syringae LPS The LPS was O-deacetylated by mild hydrazinolysis and then N-deacylated

Ngày tải lên: 23/03/2014, 13:20

... PROCHECK [38]. Trang 4the backbone and side chain of Asn34 and the side chain of Lys24 This crystal contact stabilizes the structure of the leading side of the foot loop The putative internal ... C-terminus, while Arg206 is on the top of the molecule, in the loop leaving helix 6 and it points in the opposite direction to the other basic residues (Fig 2B) The structure of the NLS is almost identical ... the side chain of Asn81 The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts Comparison with the structure of the soluble form of

Ngày tải lên: 23/03/2014, 15:21

... presented structure and showed, in particular, the configuration of ManNAc to beD From the combined the data on the structures of the isolated oligosaccharides, the overall structure of the P frisingensis ... H3 of Kdo C and H6 of Kdo D is possible only in the case of an a-configuration of residue D, linked to O4 of Kdo C, as follows from molecular modeling The unusual position of the H3 signals of ... 4Scheme 1 Structures of the isolated compounds and proposed structure of the carbohydrate backbone of P frisingensis VTT E-82164 LPS.Trang 5their a-configuration [16], while the H3 signals of Kdo

Ngày tải lên: 23/03/2014, 18:20

... 4) The structure of the C378G variant of SAP97PDZ2 was practically identical to that of C378S variant, except for the mutated residue, and because of its better resolution, only the structure of ... domains In the structure of the peptide complex, only the last four C-terminal residues of the GluR-A are visible, and align as an antiparallel b-strand in the binding groove of SAP97PDZ2 The free ... domain structures The solution structure of the second PDZ domain of PSD-95 has been determined by NMR [21], and, quite recently, coordinates of the PDZ2 domain of SAP102 crystallized in the absence

Ngày tải lên: 30/03/2014, 10:20