structural and conformational analysis of oligosaccharides

... resonances of Pro1 and Asp2 have the highest intensities in the STD spectrum, signals of Thr3 are of medium intensity, while the signals of Arg4 and Pro5 have the lowest intensity The d-protons of Pro5 ... Protons of Asp2, Thr3, Arg4 (including intra-residue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and ... STD and normal TOCSY spectrum of the PDTRP/SM3 complex are shown The peaks of Arg4 and Pro5 are so low in intensity that they do not appear at the intensity cutoff shown Signals of Pro1, Asp2 and...

Ngày tải lên: 21/02/2014, 15:20

... Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p and Pex6p involves ... function of Pex1p and Pex6p A B Fig 2 Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites. A schematic representation of the ... parts of the N-terminal fragment and⁄ or the second cassette; D C Fig 5 Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex6p on the morphological appearance of peroxisomes...

Ngày tải lên: 07/03/2014, 16:20

... residues of D3 and B contribute to the stability of the dimer The first cluster includes F70/V336 and I72/Q338 (both in b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 ... b5 strand) of D3 and I41/L304, C43/A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 ... in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 (b2 strand), I33/K294 (loop after b2 strand), I68/F334, L71/ V337 and L73/F339...

Ngày tải lên: 30/03/2014, 15:20

... Plasticity of S2–S4 specificity pockets of executioner caspase-7 revealed by structural and kinetic analysis Johnson Agniswamy, Bin Fang and Irene T. Weber Department of Biology, Molecular Basis of Disease, ... identification of the small aliphatic binding region of the S4 subsite of caspase-7. The struc- ture of caspase-7 ⁄IEPD shows only one hydrogen bond between the main chain atoms of caspase-7 and P4, and lacks ... during X-ray data collection Use of the Advanced Photon Source was supported by the US Department of Energy, Of ce of Science, Of ce of Basic... Kodandapani L, Wu JC & Grutter MG (2000)...

Ngày tải lên: 18/02/2014, 16:20

... chromophore of ABA-1, the single Trp of Ag-NPA-1 is a sensitive marker of the protein conformation and its emission reflects conformational changes after ligand binding. Analysis of FRET from the ... (K 2 0.667 [30]). A value of 1.36 was taken for the refractive index n [31]. MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after tryptic digestion of the labeled and nonlabeled Ag-NPA-1 ... good correspondence of the experimentally determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found. A theoretical prediction of the secondary structural organization of Ag-NPA-1...

Ngày tải lên: 16/03/2014, 18:20

... of the results of the mechanical and metallurgical analysis of structural steel from the World Trade Center (WTC), part of the National Institute of Standards and Technology Investigation of ... Specifications, Standards, and Sources 129 [...]... performance of WTC 1 and WTC 2 under design, service, and abnormal loads, and aircraft impact damage on the structural, fire protection, and ... thermal environment, and smoke movement in WTC 1, 2, and 7 for use in evaluating the structural performance of the buildings and behavior and fate of occupants and responders Analyze...

Ngày tải lên: 01/04/2014, 00:20

... ID(proteins(and(myogenesis( (14! 1.6! ID(proteins(and(neurogenesis( (14! 1.7! IDs(in(cancer( (16! 1.8! Properties(and(roles(of(ID2( (17! 1.9! Aim(and(Scope(of(Project( (20! CHAPTER 2: MATERIALS and METHODS ... Trang 1STRUCTURAL CHARACTERIZATION AND BIOCHEMICAL ANALYSIS OF ID2, AN INHIBITOR OF NATIONAL UNIVERSITY OF SINGAPORE 2012 Trang 3! i!ACKNOWLEDGEMENTS ... expression and purification of ID1 and ID3 HLH domains 104! Trang 9! vii!LIST OF FIGURES Figure 1: Hydrophobic core packing of bHLH-containing proteins 5! Figure 2: Cartoon representation of ID3...

Ngày tải lên: 09/09/2015, 10:20

... E47, MyoD and MASH1 through electrophoretic mobility shift assays and mutagenesis experiments of specific residues based on the structural analysis ! 21! ! CHAPTER 2: MATERIALS and METHODS ... between 1.5 – 2 mg of pure ID2 per litre of bacterial culture The chromatography profiles of all 3 constructs were virtually identical so a representative set of profiles is shown in Figure ... of ID2 3) Analyze the structure of ID2 and look at similarities and differences to other HLH-containing proteins including ID3 ! 20! 4) Determine differences in binding between ID1, ID2 and...

Ngày tải lên: 09/09/2015, 10:20

... R and Fitch, W M (1997) "A natural classification of the basic loop-helix class of transcription factors", Proceedings of the National Academy of helix-Sciences of the United States of ... bonds such as those of Y71 (chain A) and Q76 (chain B) completely abolished soluble protein production and was required for proper folding of the monomer and hence, the stability of the dimer In ... diameter of the density, the coordination of the surrounding residues and the fact that the crystals were grown in a high concentration of potassium The ion was found in the loops of both monomers and...

Ngày tải lên: 09/09/2015, 10:20

... M00are respectively real and imaginary parts of the modulus, and C0¼ ε0A/L, with A as the area of the sample, L as the thickness of the sample, andε0as the permittivity of the free space (8:854 ... a systematic study of the morphology, structure, dielectric and impedance performance of LiNi1xy0.02CoxZnyMg0.02O2(x¼ y ¼ 0.0, 0.02 and 0.04) cathode materials Here Zn and Mg acted as the ... temperatures The mixture of the starting ma-terials was sufficiently mixed and after grinding the powder, it was then heat treated in air at 500C for 5 h and it was again ground and mixed, and calcined at...

Ngày tải lên: 17/03/2021, 20:22

... on the structure of monosaccharides and disaccharides as the basis of the structural and conformational analysis of oligosaccharides and polysaccharides The fundamentals of SAXS and NMR together ... III STRUCTURAL AND CONFORMATIONAL ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES The structural characterization of simple homoglucans is mainly introduced in this section The structural and ... the structural and conformational characterization of oligosaccharides and polysaccharides, particularly in solution, by taking the examples of the homoglucans composed of dierent modes of glucosidic...

Ngày tải lên: 23/05/2014, 13:14

... conditions of a supramolecular structural organization (aggregation) of CPP and Ca2+ was studied by a laser light scattering technique capable of establishing the dimensions (hydrodynamic radius) and ... markers [alkaline phosphatase (ALP) and sucrase-isomaltase] and the rate of proliferation, and by electron-microscopic examination As shown in Fig 1A, the levels of ALP and sucrase-isomaltase in RPMI ... solution of CPP In contrast, the solution of the same CPP in phosphate-free or phosphate-containing KRH with no Ca2+ showed a remarkable increase of scattered light, of the order of 10 times that of...

Ngày tải lên: 16/03/2014, 05:20

... and at the same time lyric The surpassing charm of his rendering of the romantic beauty and joy of life and the profundity of his presentation of its tragic side are what make Shakespeare's plays ... with, and even due to the impression of waste which puts strong influence on the hearts and minds of the audience and makes them continuously think about the story and its message The strength of ... napkin is too little He puts the handkerchief form him, and she drops it Othello’s rejection of Desdemona's offer of her handkerchief is his intensified rejection of Desdemona herself All Iago has...

Ngày tải lên: 22/12/2013, 13:11

... (1996), Leland (1998), and others These papers take the form of the securities as given and derive the effect of capital structure on the incentives of the manager, debt holders, and shareholders, ... II, which is an expanded version of Table I We not include a row for λ, but it is easy to see that λ and σ have the same effects on W0, W*, and b(W*), and the effect of λ on CL and D can be found ... 15%, and standard term loans for 22%, of corporate borrowing for this period See Acharya et al (2002) for an analysis of the impact of these options held by equity holders on credit spreads and...

Ngày tải lên: 12/01/2014, 22:16

... composition of the new debt obligations is part of the restructuring agreement (Sturzenegger and Zettelmeyer 2005) 14 and to the laws of motion for the stock of perpetuities of short and long duration: ... probability of default and the level and maturity composition of debt issuances are endogenous variables The outline of the paper is as follows Section documents the dynamics of the spread curve and ... structure of debt issuances and the term structure of spreads as reflecting a trade-off between insurance benefits of long-term debt and liquidity benefits of short-term debt, both due to the presence of...

Ngày tải lên: 16/02/2014, 02:20

... number of sources of randomness, are allowed? One of the main results of this paper is that, at this level of generality, uniqueness of the martingale measure implies only that the set of hedgeable ... the existence of a martingale measure implies absence of arbitrage, and we end the section by investigating existence and uniqueness of martingale measures We make the following standing assumption ... (25) and A, S and D are defined as in (6) Proof I First of all it is easy to see (using the Itˆ formula) that a measure Q o is a martingale measure if and only if the bond dynamics under Q are of...

Ngày tải lên: 16/02/2014, 02:20

... Qcr10p) 1910 On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc1 complex may occur due to the binding of ISP and Qcr10p, possibly leading ... insertion of ISP into an immature bc1 complex and that the association of the Qcr9p subunit with the bc1 complex precedes the binding of ISP In fact, the direct absence of ISP (DISP), or the block of ... conclude that Qcr6p and Qcr9p are not required for Bcs1p binding and that the binding of ISP and Qcr10p is subsequent to that of Bcs1p Previous studies have suggested that the insertion of ISP into the...

Ngày tải lên: 18/02/2014, 08:20

... sheets, subjected to TLC and quantication of radiolabelled ADP and AMP The kinetics of [3H]AMP and [3H]ADP formation in the absence of tRNAGlu (black symbols) or of both L-Glu and tRNAGlu (grey symbols) ... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of ... selected GluRS sequences and identication of the limited chymotryptic cleavage sites Fig S8 Analysis of the kinetics of proteolysis of Mt- and Ec-GluRS Fig S9 Minimal models of the proteolytic events...

Ngày tải lên: 07/03/2014, 03:20

... glycoforms, suggesting the presence of alternate sialylated structures In this study we describe the identification and structural analysis of a Sial-lNnT unit in H influenzae LPS MATERIALS AND ... lgÆmL)1) or tetracycline (4 lgÆmL)1) Structural analysis and purification of LPS LPS was prepared for structural analysis from cells harvested after growth on batches of 20 BHI plates, supplemented ... acquired with a NOE mixing time of 800 ms, spin lock time of 80 ms, and 20480 transients for a duration of 21 h Electrophoretic analysis of LPS LPS was prepared and analysed by tricine-SDS/PAGE...

Ngày tải lên: 23/03/2014, 21:21

... level of a very low whisper, would be picked up by it … p The heirs of the French, English, and American revolutions had partly believed in their own phrases about the rights of man, freedom of ... listing of unedited net asset values of publicly traded investment fund shares , reported by the companies as of Friday 's close p Conspicuous by its absence is California p Out of the mouths of revolutionaries ... Three types of postposing: n Existential there In Ireland’s County Limerick, near the River Shannon, there is a quiet little suburb by the name of Garryowen, which means “Garden of Owen” n Presentational...

Ngày tải lên: 24/03/2014, 19:20