salt tolerance in transgenic plants

... physiological indices in WT and transgenic lines at different time points after stress treatment were measured Under salt stress, the decrease in chlorophyll content was greater in WT plants than in TaNAC29-overexpression ... 9overexpression lines following salt stress (Fig 6f ), indi-cating that TaNAC29-overexpression lines scavenged more H2O2 POD activity in WT plants and TaNAC29-overexpression lines significantly increased, ... 6overexpression lines had increased tolerance to saltand drought stresses TaNAC29-overexpressing plants exhibit ABA-hypersensitive response Whether salt and drought tolerances of TaNAC29-over-expression line

Ngày tải lên: 26/05/2020, 20:10

... of OsCYP2 in the response of rice seed-ling to salt stress is required, but also it can enhance salt tolerance in transgenic rice seedlings by controlling ROS levels In addition to salt stress, ... C) Although OsCYP2 expression was inhibited in two transgenic lines and was induced in wild type under salt stress, salt-stressed seedlings of two transgenic lines showed close or higher levels ... signaling Results: Phenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance

Ngày tải lên: 11/08/2014, 11:21

... seedlings harboring pART-P450-cSUC2-BAR (referred to as OCP lines; Overexpressing Cytochrome P450SU1) In plants displaying the most severe phenotype, these aberrations included severe stunting, ... (encoding ubiquitin) by semi-quantitative RT-PCR in 17 OCP lines, as well as in WT and cSUC2 lines, and those transformed with pART-uidA-BAR (uidA lines) (Figure 2) In Figure 2, the OCP lines ... flowering (Table 1) Plants overexpressing P450SU1 also had fewer siliques and individual siliques had fewer seeds, resulting in an overall lower seed yield (Figure 3A, B) To gain insight into

Ngày tải lên: 11/08/2014, 11:22

... lines had higher CAT activity For the ILs, salt treatment significantly decreased activity in 71% of the lines, increased it in 23% of the lines and had no effect in the remaining 6% of the lines ... [12] have improved tolerance to abiotic stresses such as salinity Salt tolerance can be defined as the ability of plants to survive and maintain growth under saline conditions Plants have three ... decreases in POX activity were seen in 33% of the ILs In contrast, increases in activity were observed in 59% of the ILs No significant change in activity was seen in 8% of the lines The greatest increase

Ngày tải lên: 12/08/2014, 03:21

... enriched in the salt gland-rich tissue, a Dehydrin gene (AoDHN1) showed nearly 6-fold increase in expression Dehydrins are known to be involved in Trang 10stress-specifically in salt glands as indicated ... Protein NIP Nod26-like Intrinsic Protein SIP Small-basic Intrinsic Protein SOS Salt Overly Sensitive NHX Sodium Hydrogen Exchangers BSA bovine serum albumin YFP Yellow Fluorescent Protein ... Chapter 1: Introduction 2 1.1 Salt and soil salinity 2 1.2 Status of mangrove forest in Singapore 5 1.3 Salt balance in mangrove plants 6 1.4 Effect of salinity on growth and development of plants

Ngày tải lên: 09/09/2015, 08:13

... regulating diverse signalling pathways against salt stress in plants In the present study, we have developed transgenic pigeon pea plants with marker free gene Psp68 for salinity tolerance Since ... investigation, decrease in RWC was observed in both WT and transgenic plants with salt treatment but decline in RWC was more in wild-type plants under 75 mM NaCl stress as compared to transgenic plants Stress ... these days to impart salinity tolerance in various crop plants Transgenics for salinity tolerance is mainly focused on introduction of genes that encode ion transport proteins, compatible organic

Ngày tải lên: 14/01/2020, 03:56

... analyses V vinifera protein sequences of interest were obtained from EnsemblPlants using the 12xV1 gene IDs V vinifera amino acid sequences were used as a query in a protein-protein BLAST (blastp) ... determine their role in chloride transport in grapevine and other plants Keywords: ABA signalling, ACA, CAX, mRNA, Salt overly sensitive (SOS), Woody perennial Background Grapevine (Vitis vinifera ... homeosta-sis by using grapevine as a model species to elucidate genes that underpin the Cl− exclusion trait in plants in general Trang 3Preparation of rooted-leaves Grapevine, being a woody perennial

Ngày tải lên: 27/05/2020, 00:09

... [48] were crossed with the transgenic plants overexpressing GmMYB73 (OE-5) plants, and GUS staining was examined in homozygous GL2p::GUS/GmMYB73 transgenic plants In the GL2p:: root, shoot apex ... stem (Figure 2c, d) GUS staining was strongly inhibited staining were barely detectable in flower buds, leaf and stem of these plants (Figure 2d) GUS staining was only plants (Figure 2c) GUS activity ... fluorescence in YFP indicates positive interactions (c) GL2 promoter activity was inhibited by GmMYB73 in aerial parts but partially suppressed in roots Left: GUS staining in whole seedlings; right:

Ngày tải lên: 27/05/2020, 01:32

... traits, the effective sampling method, and finally to find QTLs contributing to improving salt tolerance in rice Thirty-seven rice varieties were screened for salt tolerance using a hydroponic system ... were used in sampling method experiment in phytotron In the control, Yoshida solution was used continuously during the experiment In the salt treatment, Trang 8were tested including cutting method ... harboring those salt tolerance genes Salinity tolerance in rice is regulated by multiple genes Expression of salinity tolerance is the result of many physiological and biochemical activities in plants

Ngày tải lên: 04/04/2021, 23:49

... Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping Mol Breeding 3, 269 –277. Foolad, M R., F Q Chen, and G Y Lin, 1998: RFLP mapping of QTLs conferring salt ... alkali at germination and early seedling stages in rice Acta Agron. Sin 34, 1719 –1727. Chinnusamy, V., A Jagendorf, and J K Zhu, 2005: Understanding and improving salt tolerance in plants Crop ... biochemical indicators of salinity tolerance in plants Plant Sci 166, 3 –16. Ashraf, M., and J W O’Leary, 1994: Ion distribution in leaves of varying ages in salt-tolerant lines of alfalfa under salt

Ngày tải lên: 07/10/2021, 01:20

... abundant in desiccated tissues than in hydrated tis-sues Inosine, a purine nucleoside containing the purine-base hypoxanthine and the sugarD-ribose, accumulated during desic-cation in Selaginella ... representing 103 unique proteins with unknown functions were identified (Wang et al., 2010) The proteins down-regulated in Selaginella tamariscina during dehydration included proteins involved in photosynthesis, ... Selaginella tamariscina during dehydration included RuBisCO large sub unit, chlorophyll a/b binding protein, and oxygen evolving complex protein (Wang et al., 2010) During dehydration, LEA proteins

Ngày tải lên: 01/11/2022, 09:49

... domain binded the nucleotide sugar and the B domain binded the acceptor substrate, together forming a viable catalytic center14,15 Moreover, there were two N-terminal putative zinc finger domains ... shoot induction medium (MS medium containing 2 mg/L ZT and 0.01 mg/L IAA) for two days in darkness The incubated explants were immersed into the Agrobacterium tumefaciens solution for 4–8 min (to ... expression in the RNAi plants in relation to the control plants Phenotypes of the RNAi transformed plants In order to observe the growth of control and knock-down plants, the germination performance

Ngày tải lên: 04/12/2022, 14:56

... region which Transgenic line 3-weeks post inoculatuin *1 4-weeks post inoculatuin *1 * 5-weeks post inoculatuin *1 * Table 2 PSTVd infection assay performed on the selected transgenic lines of N ... higher levels of resistance in the preliminary experiments *1Number of plants infected/inoculated *2Ten plants per transgenic line were inoculated However, since some of the plants grew poorly for ... the T0 transgenic plants were self-pollinated in order to obtain homozygous T2 and T3 plants Two weeks after sowing T3 seeds, transgenic seedlings were transferred to rockwool and grown in a plant

Ngày tải lên: 04/12/2022, 16:21

... leaves of citrus, being strongly induced in leaves of WS + HS Cleopatra plants, showing the greatest SA levels In general, abiotic stress induced higher SA build-up in Cleopatra than in Carrizo and ... important role in abiotic stress-induced signaling and tolerance [11, 18] Particularly, it has been proposed that SA may induce ther-motolerance in several plant species [19–22] Studies in Arabidopsis ... signaling through inhibition of PP2Cs and activate influx Ca2+ channels, increasing its cytosolic con-centration Subsequently, this Ca2+ accumulation contrib-utes to inhibit ion influx into guard

Ngày tải lên: 22/05/2020, 05:14

... transgenic lines containing the ProCiNAC4::GUS construct was used to determine the GUS expression Histochemical GUS staining was detected, with varying intensity, in many tissues of the transgenic plants ... sub-domains (a-e) in the NAC binding domain are indicated by underlines Identical amino acids are indicated by white letters on a black background Trang 4wild-type and the transgenic plants throughout ... the ABA-induced inhibition of seed germination Overexpression ofCiNAC3 and CiNAC4 increased the expression ofAtMYB2 Because the CiNAC3 and CiNAC4 transgenic plants showed a high germination rate

Ngày tải lên: 26/05/2020, 21:59

... ERD10, KIN1, and KIN2, analyzed in this study, under stress conditions. The differentially expressed genes in transgenic plants that encode proteins that probably function in stress tolerance include ... stress tolerance in transgenic Arabidopsis plants and may be used to engineer stress tolerance in crop plants. Furthermore, for a better understanding of the functions of TOP6 genes, transgenic ... homologs. The amino-acid sequence analysis of rice TOP6 pro- teins also predicted several potential putative phos- phorylation sites for casein kinase II, protein kinase C, tyrosine kinase, histidine kinase,...

Ngày tải lên: 30/03/2014, 10:20

... of recombinant immunoglobulin chains in plants is significant in demonstrating the suitability and potential versatility of transgenic plants for producing a variety of mammalian proteins. Protein translocation ... IgG- expressing plants. Again this is consistent with the model for BiP in assisting protein folding and increasing throughput of the ER. In mammals, BiP associates with both Ig light and heavy chains ... binding transiently in the ER to Ig chains during protein folding and processing. The immunoblot results matched those found by Northern blot, in that most BiP protein was immunoprecipitated in...

Ngày tải lên: 31/03/2014, 08:20

... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shown that mutations in BRI1 ... et al. 2002) were determined as positive regulators in ABA signaling. Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine– threonine type protein kinase, was isolated from ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNA- binding domain (Sakai et al. 1998) called a GARP domain (Riechmann et al. 2000) and the proline- and glutamine-rich...

Ngày tải lên: 14/02/2014, 12:20

... vulgare) by rubbing the virus suspension in 100 m M phosphate buffer (pH 7.2) containing 2% poly(vinylpyrrolidone) in the presence of Carborundum powder. Inoculated plants were maintained in a greenhouse ... cDNA encoding SNA; RIP, ribosome-inactivating protein; SNA, Sambucus nigra agglutinin; SNLRP, Sambucus nigra lectin-related protein; TMV, tobacco mosaic virus. Enzyme: ribosome-inactivating protein ... are located in domain 1, and two others (linking Cys147- Cys162 and Cys188-Cys205, respectively) are located in domain 2. The sugar-binding activity of SNA-If relies on two carbohydrate-binding sites...

Ngày tải lên: 31/03/2014, 23:20

... and in ux systems are very impor- tant for cellular ion relationships in natural conditions. Increasing in ux, decreasing efflux or both can maxi- mize K + uptake to maintain K + homeostasis in plants [32,33]. ... GUS activities in the transgenic seedlings or different tissues was determined after the transgenic plants had been incubated overnight at 37 °Cin1mgÆmL )1 5-bromo-4-chloro-3-indolyl-glucuronic ... Histochem- ical staining indicated that AtHELPS is mainly expressed in the young seedlings and vascular tissues of leaves and roots. Using both helps mutants and overexpression lines, we observed that, in the...

Ngày tải lên: 14/02/2014, 18:20