engineering salt tolerance in plants

... expression on salt tolerance in rice seedlings Overex-pression of OsCYP2 conferred salt tolerance in trans-genic rice seedlings Although OsCYP2-transtrans-genic seedlings did not predominate over ... of OsCYP2 in the response of rice seed-ling to salt stress is required, but also it can enhance salt tolerance in transgenic rice seedlings by controlling ROS levels In addition to salt stress, ... proline residue and the preceding residue, and functions as a molecular chaperone involved in protein folding, and refolding of denatured proteins OsCYP2 possesses seven residues, including His-61,

Ngày tải lên: 11/08/2014, 11:21

... lines had higher CAT activity For the ILs, salt treatment significantly decreased activity in 71% of the lines, increased it in 23% of the lines and had no effect in the remaining 6% of the lines ... [12] have improved tolerance to abiotic stresses such as salinity Salt tolerance can be defined as the ability of plants to survive and maintain growth under saline conditions Plants have three ... decreases in POX activity were seen in 33% of the ILs In contrast, increases in activity were observed in 59% of the ILs No significant change in activity was seen in 8% of the lines The greatest increase

Ngày tải lên: 12/08/2014, 03:21

... enriched in the salt gland-rich tissue, a Dehydrin gene (AoDHN1) showed nearly 6-fold increase in expression Dehydrins are known to be involved in Trang 10stress-specifically in salt glands as indicated ... Protein NIP Nod26-like Intrinsic Protein SIP Small-basic Intrinsic Protein SOS Salt Overly Sensitive NHX Sodium Hydrogen Exchangers BSA bovine serum albumin YFP Yellow Fluorescent Protein ... Chapter 1: Introduction 2 1.1 Salt and soil salinity 2 1.2 Status of mangrove forest in Singapore 5 1.3 Salt balance in mangrove plants 6 1.4 Effect of salinity on growth and development of plants

Ngày tải lên: 09/09/2015, 08:13

... analyses V vinifera protein sequences of interest were obtained from EnsemblPlants using the 12xV1 gene IDs V vinifera amino acid sequences were used as a query in a protein-protein BLAST (blastp) ... determine their role in chloride transport in grapevine and other plants Keywords: ABA signalling, ACA, CAX, mRNA, Salt overly sensitive (SOS), Woody perennial Background Grapevine (Vitis vinifera ... homeosta-sis by using grapevine as a model species to elucidate genes that underpin the Cl− exclusion trait in plants in general Trang 3Preparation of rooted-leaves Grapevine, being a woody perennial

Ngày tải lên: 27/05/2020, 00:09

... traits, the effective sampling method, and finally to find QTLs contributing to improving salt tolerance in rice Thirty-seven rice varieties were screened for salt tolerance using a hydroponic system ... were used in sampling method experiment in phytotron In the control, Yoshida solution was used continuously during the experiment In the salt treatment, Trang 8were tested including cutting method ... harboring those salt tolerance genes Salinity tolerance in rice is regulated by multiple genes Expression of salinity tolerance is the result of many physiological and biochemical activities in plants

Ngày tải lên: 04/04/2021, 23:49

... Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping Mol Breeding 3, 269 –277. Foolad, M R., F Q Chen, and G Y Lin, 1998: RFLP mapping of QTLs conferring salt ... alkali at germination and early seedling stages in rice Acta Agron. Sin 34, 1719 –1727. Chinnusamy, V., A Jagendorf, and J K Zhu, 2005: Understanding and improving salt tolerance in plants Crop ... biochemical indicators of salinity tolerance in plants Plant Sci 166, 3 –16. Ashraf, M., and J W O’Leary, 1994: Ion distribution in leaves of varying ages in salt-tolerant lines of alfalfa under salt

Ngày tải lên: 07/10/2021, 01:20

... strategies forimproving heat tolerance in plants are involved in cellular repair, rescue, cleanup and/or protection during the stress and Trang 205 FREEZING STRESSPlants growing in temperate and ... accumulation also increases freezing tolerance However, much informa-tion is not available regarding the freezing injury and tolerance mechanisms againstfreezing stress Recently much interest has ... complexicity, the mechanisms underly-ing salt tolerance are to be investigated in detail Dajic in Chapter 3 deals in detail themolecular basis of salt tolerance in addition to related physiological,

Ngày tải lên: 12/11/2022, 09:01

... in germinating seeds (Bush et al.,1986).In the cytosol, calcium binding proteins known as calcium modulated proteinslike calmodulin (Snedden and Fromm, 2001), calmodulin binding proteins (Reddy ... oxidizing complex I or complex II substrates FEBS Lett 451, 347-350. Breusegem, F.V., Montagu, M V and Inzé, D (2002) Engineering stress tolerance in maize In: Oxidative stress in plants (Inzé, ... cysteinyl glycine) plays a pivotal role in minimizing cellular disfunc-tion, arising through stress induced redox perturbation (Vernoux et al., 2002) Interest inthe benefits of genetically engineered

Ngày tải lên: 12/11/2022, 09:01

... domain binded the nucleotide sugar and the B domain binded the acceptor substrate, together forming a viable catalytic center14,15 Moreover, there were two N-terminal putative zinc finger domains ... shoot induction medium (MS medium containing 2 mg/L ZT and 0.01 mg/L IAA) for two days in darkness The incubated explants were immersed into the Agrobacterium tumefaciens solution for 4–8 min (to ... expression in the RNAi plants in relation to the control plants Phenotypes of the RNAi transformed plants In order to observe the growth of control and knock-down plants, the germination performance

Ngày tải lên: 04/12/2022, 14:56

... protein involved in inducing double-strand breaks to initiate meiotic recombination in eukaryotes [8,9] Their exist-ence has also been shown in plants [10–14] In contrast with other eukaryotes, plants ... putative phos-phorylation sites for casein kinase II, protein kinase C, tyrosine kinase, histidine kinase, cAMP-dependent and cGMP-dependent protein kinases, and putative N-gly-cosylation, N-myristoylation ... auxin and cytokinin, indica-ting their role in cell proliferation and hormone signa-ling The interaction of OsTOP6A3 with OsTOP6B along with their similar expression patterns and local-ization in

Ngày tải lên: 30/03/2014, 10:20

... 9overexpression lines following salt stress (Fig 6f ), indi-cating that TaNAC29-overexpression lines scavenged more H2O2 POD activity in WT plants and TaNAC29-overexpression lines significantly increased, ... those observed in JUB1- and ATAF1-over-expressing plants [5, 7] Next, TaNAC29-overexpression plants were examined for tolerance to salt stress Twenty-five-day-old seed-lings, grown in soil, were ... 6overexpression lines had increased tolerance to saltand drought stresses TaNAC29-overexpressing plants exhibit ABA-hypersensitive response Whether salt and drought tolerances of TaNAC29-over-expression line

Ngày tải lên: 26/05/2020, 20:10

... applied in breeding stress-tolerant plants and gen-etically engineering plants with improved properties, which are suitable for growing on marginal lands Results Sequencing and data analysis In the ... factors, and the remaining targets included ABC transporter, F-box protein, proteins involved in sRNA biogenesis and function, and two laccases function-ing in lignin biosynthesis In addition, 290 ... during salt treatment and between the shoots and roots were also investigated This study con-tributed in elucidating the molecular mechanisms of salt tolerance in S europaea Specific miRNAs in

Ngày tải lên: 26/05/2020, 23:56

... GT-4 encoded a protein of 372 amino acids and the protein had a trihelix DNA binding domain in the N-terminus and a variable C-terminus Arabidopsis seedlings were treated with salt stress and the ... box in the Cor15A promoter and activates its expressions A B3 and AP2/ERF domain containing protein TEM2 was identified to interact with GT-4 and jointly regulate salt tolerance Various binding ... plants overexpressing GT-4 exhibits salt tolerance com-pared to Col-0 We further identified a B3 and AP2/ERF domain-containing protein (TEM2) that interacted with GT-4 Loss function of TEM2 in

Ngày tải lên: 27/05/2020, 00:38

... drought tolerance of plants Auxins induce new root formation by breaking root apical dominance induced by cytokinins As a prolific root system is vital for drought tolerance, auxins have an indirect ... resistance mechanisms in plants, and also sum up the advances regarding to drought response in plants tolerance in plants productivity is considerably reduced owing to the changing climate conditions ... damage of wheat plants in pots under drought, Plant Sci., 169: 313–321 Hallajian, M.T 2016 Mutation Breeding and Drought Stress Tolerance in Plants In: Drought Stress Tolerance in Plants,

Ngày tải lên: 02/07/2020, 23:39

... possessing the cupin do-main(s) in their sequence were found in various M-QTL the seedlings According to the previous reports, cupin domain might play a role in improving the seed germin-ation in ... meta-analysis Findings of this study provide valuable information on the genes and pathways in-volved in salinity tolerance in rice Results Salinity tolerance associated Meta-QTLs in rice A total ... up-regulated in the plants expressing the cytosolic peroxidase genes have addition, there was a hydrolase coding gene belonging to the alpha/beta fold family domain containing pro-tein in Chr3:M-QTL3that

Ngày tải lên: 28/06/2021, 20:42

... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shown that mutations in BRI1 ... et al. 2002) were determined as positive regulators in ABA signaling. Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine– threonine type protein kinase, was isolated from ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNA- binding domain (Sakai et al. 1998) called a GARP domain (Riechmann et al. 2000) and the proline- and glutamine-rich...

Ngày tải lên: 14/02/2014, 12:20

... effective compatible osmolytes in plants subject to salt stress are GB, b -alaninebetaine, prolinebetaine, choline- O -sulphate, hydroxypro- linebetaine, and pipecolatebetaine (Ashraf and Fig. 1.1 ... (2007) Exogenous proline and glycinebetaine increase NaCl-induced ascorbate- glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright ... including the luminal binding protein gene BiP is induced. The under- lying mechanisms of UPR in yeasts and mam- mals have been well researched (Rutkowski and Kaufman 2004 ) , although those in...

Ngày tải lên: 14/03/2014, 10:20

... expressed in plants. Heavy-chain derivatives are shown in gray and light-chain derivatives in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the ... in gray and light chains in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate the variable ... of other valuable proteins using this system, including avidin and β-glucuronidase. Initial findings for the expression of a secretory IgA in maize showed that the four chains were expressed, directed...

Ngày tải lên: 25/10/2013, 05:20

... others being delphinidin, peonidin, pelargonidin, petunidin, and malvidin. Of the flavonols, quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetin being the predominant ... organisms. The methods for increasing, preventing, or redirecting flux into or within the path- way include increasing levels of a rate-limiting biosynthetic enzyme, inhibition of the activity ... third predominant flavonoid group in fruits is proanthocyanidins and their monomer units, catechins (procyanidin) or gallocate- chins (prodelphinidins). Delphinidin-derived anthocyanins are known...

Ngày tải lên: 25/10/2013, 05:20

... and in ux systems are very impor- tant for cellular ion relationships in natural conditions. Increasing in ux, decreasing efflux or both can maxi- mize K + uptake to maintain K + homeostasis in plants [32,33]. ... Histochem- ical staining indicated that AtHELPS is mainly expressed in the young seedlings and vascular tissues of leaves and roots. Using both helps mutants and overexpression lines, we observed that, in the ... progress in determining the molecular mecha- nism of the regulation of K + uptake in plants has been made [10,11,40]. AKT1 was first reported to be expressed in roots and involved directly in the mineral...

Ngày tải lên: 14/02/2014, 18:20