ribosomes from trypanosomatids unique structural and functional properties

... unstructured and highly flexible and protrudes from the oligomeric structure Both the polar-ity and flexibilpolar-ity of the C-terminal extension are important for the mainte-nance of sHsp solubility and ... small heat shock protein 25 are critical for structural and functional integrity Amie M Morris1, Teresa M Treweek2, J A Aquilina1, John A Carver3and Mark J Walker1 1 School of Biological Sciences, ... in the C-terminal extension at positions 190, 199 and 204 were each replaced with alanine The mutants were found to be structurally altered and functionally impaired Although there were no significant...

Ngày tải lên: 07/03/2014, 04:20

... 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpress-ing IL-1b (Fig 5, lanes 3 and 4, upper bands) ... and encoding MCPIP [2] This protein con-sists of 599 amino acids, and has a putative nuclear localization signal and two proline-rich potential acti-vation domains, one between residues 100 and ... mice suffered from severe anemia, had increased serum immunoglobulin levels and showed auto-antibody production Transcriptome com-parison of LPS-treated macrophages from wild-type and Zc3h12a-deficient...

Ngày tải lên: 18/02/2014, 14:20

... responses to 12-oxo-phytodienoic acid are governed by its specific structural and functional properties Christine Bo¨ttcher1and Stephan Pollmann2 1 CSIRO Plant Industry, Adelaide, Australia 2 ... but also directly to enzymes and transcription factors OPDA: an independent regulator of gene expression Given the functional and structural differences between OPDA and JA, it is exciting to presume ... of structurally related compounds and their role in cellular stress responses is a very intriguing and challenging matter of actual research The latter com-pounds comprise oxidized lipids and...

Ngày tải lên: 16/03/2014, 02:20

... variety of structural and functional properties from either database annotations or computational predic-tion methods and analyzed SNP A/S ratios for different classes of proteins and residues ... A/S ratios) Results: Using data from the dbSNP database, we conducted the first large-scale survey of SNP A/ S ratios for different structural and functional properties We confirmed that the ... between SNP A/S ratios and other variables provide valuable insights into the natural selection of various structural or functional properties, particularly for human-specific genes and constraints...

Ngày tải lên: 14/08/2014, 08:21

... between R81 and P82 and between G90 and I91. A similar cis peptide between G93 and V94 is found in Tm SurE also. Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium A. Pappachan 1 , H. S. Savithri 2 and M. R. N. Murthy 1 1 ... the secondary structural elements of St SurE. Arrows represent b-strands, while small and large cylinders represent 3 10 -helices and a-helices, respectively. Numbers shown indicate the starting and ending...

Ngày tải lên: 18/02/2014, 14:20

... formation depends upon contact of b-strand 10 from the C-ter-minal extensions of monomers A and D with b-strands 4 and 8 in the a-crystallin domain of monomers C and B, respectively (Fig 8) A more ... Hsp16.5 from the archaeon, Methanococcus jannaschii, and Hsp16.9 from wheat, Triticum aestivum, assemble monodisperse oligomers and they have been crystallized, revealing important sHSP structural ... F112R, Y116D and R110G, with the latter two not significantly different from one another Chap-eroning of insulin by p26 purified from Artemia [45] and E coli was very similar, whereas BSA and IgG at...

Ngày tải lên: 07/03/2014, 12:20

... Xuhua, Ms Sunita and, Mr Jobi and the rest of the lab mates for the valuable discussion and friendship and the present and former members of Functional Genomics Laboratory as well as Structural Biology ... Trang 1STRUCTURAL AND FUNCTIONAL STUDIES OF VP9, A NOVEL NONSTRUCTURAL PROTEIN FROM WHITE SPOT SYNDROME VIRUS LIU YANG (B.Sc., Xiamen University) ... leading to its prevention and treatment being the utmost importance for this disease The aim of this thesis is to provide structural and functional insights into one of the nonstructural viral proteins...

Ngày tải lên: 14/09/2015, 09:57

... Trang 1A comparative study on stability and functional properties of the proteins isolated from yellowfin tuna (Thunnus albacares Article · December 2016 CITATIONS ... All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. Trang 2ﻪﺴﯾﺎﻘﻣ ﯽﮔﮋﯾو ي يﺎﻫ ﻦﯿﺌﺗوﺮﭘ يدﺮﺑرﺎﮐ ... Some of the authors of this publication are also working on these related projects: Extracting protein from tuna processing by-products using pH-shift method View project Amir Reza Shaviklo Animal Science Research Institute of Iran (ASRI)...

Ngày tải lên: 31/05/2017, 02:24

... physicochemical properties (chemical composition, molecular weight, particle size of protein) and functional properties (solubility, water and oil holding capacity, emulsifying ability and stability, ... influenced by pH, temperature and salt Food hydrocolloids, 23(1), 72-81 5 Ahn, H., Kim, J., & Ng, P (2005) Functional and thermal properties of wheat, barley, and soy flours and their blends treated ... D., & Davila-Ortiz, G (2002) Functional properties of flours and protein isolates from Phaseolus lunatus and Canavalia ensiformis seeds Journal of Agricultural and Food chemistry, 50(3), 584-591...

Ngày tải lên: 07/06/2024, 16:26

... hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... (B) HPRT and hypoxanthine (C) PRTFDC1 and guanine (D) HPRT and guanine. Table 2 Kmand Vmaxvalues for Hx and G in the presence of 1 m M PRPP, determined using the DE-81 filter paper assay and tritium-labeled ... S-(dimethylarsenic)cysteine Library files and coordinates for GMP and S-(dimethylarsenic)cys-teine were obtained from the HIC-UP ligand database [36] and generated at the prodrg server [37] All...

Ngày tải lên: 15/02/2014, 01:20

... signature), 218 nm (b strand signature), 202–204 nm (random coil signature) In WhiB5 and WhiB6, the structure was dominated by a helices and b strands and the propor-tion of these structural elements ... biochemical and biophysical properties of WhiB2, WhiB5, WhiB6 and WhiB7 of M tuberculosis and also compare the prop-erties of all seven WhiB proteins We show that, simi-lar to WhiB3 and WhiB4, ... b-strand and random coil features However, the proportion of each feature varied among WhiB proteins, as evident from the difference in negative molar ellipticity at specific wavelengths, i.e 208 and...

Ngày tải lên: 18/02/2014, 12:20

... chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and from O3 to the ... no water molecule fix-ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E A phosphate ion was found in the donor ... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and...

Ngày tải lên: 18/02/2014, 16:20

... PDGF-a and -b on chromosomes 7 and 22 [17,18], and PDGF-c and -d on chromosomes 4 and 11 [19], respectively The genomic organization of the pdgf genes is quite similar, although PDGF-c and -d ... in PDGF-c (exons 5 and 6) and PDGF-d (exons 6 and 7) encoding the cystine knot motifs resemble the corresponding exons in the PDGF-a and -b(exons 4 and 5) genes Both in PDGF-a and -b, exon 6 encodes ... While PDGF-A and -B can form both homo- and heterodimers (PDGF-AA, -AB, -BB), PDGF-C and -D exist only as homodimers (PDGF-CC and -DD) The full-length PDGF-C and -D monomers are 54–55 kDa and 49–56...

Ngày tải lên: 19/02/2014, 07:20

... bacterial growth were purchased from Fluka (Milan, Italy) T4 DNA ligase and Taq polymerase were from Stratagene (La Jolla, CA, USA), and restriction enzymes and dNTPs from Promega Italia (Milan, ... conformation, which in turn reflects its reactivity and stability The functional differences between the two MTs are due to specific structural properties and may be related to the different lifestyles ... 40 and 80 m M (lanes 2 and 3) for 3 h MTs were also treated for the same period with a reducing agent (dithiothreitol) at 40 and 80 m M (lanes 4 and 5) To reduce aggregation, MT samples were handled...

Ngày tải lên: 19/02/2014, 07:20

... year old boys and girls who are overweight range from 4% to 35% across countries and regions Canada, Greenland, Malta and the United States have among the highest rates Boys, and those from less ... and eating habits in several ways: • • • Recall of intake of fruits and vegetable and how much and how many servings per day; recall of sugar intake and how many added sugar drinks or foods and ... children 1-10 years of age and adolescents 11-18 years from 1965-1991 and found a decline in breakfast consumption, especially for 15-18 year olds from 89.7% to 74.9% in boys and from 84.4% to 64.7%...

Ngày tải lên: 21/02/2014, 23:20

... a decision to act and hence intention becomes a very powerful tool for need assessment and the prediction of service use Theories and results of health and social psychology from the last decades ... between psychological distress and intention and table reports the association between intention and participation But as shown in table the association between distress and participation adjusted ... association between GHQ-12 and intention among the 602 female teachers and for the highest tercile of the MBI-EE The data from the smaller group of 339 male teachers and the hence less powerful...

Ngày tải lên: 20/06/2014, 00:20

... amount of both standard and non-standard test data is available Table presents the lowest reported standard and non-standard effect values (according to the Wikipharma database [6] and the environmental ... Recommended and mandatory Yes Recommended, mark between and 10 No Recommended and mandatory, mark between and Yes Information on how to summarize the evaluation Not stated Stated Stated Stated and calculated ... significantly different from standard test methods can never be put in the highest reliability category Durda and Preziosi [11] distinguish between mandatory and optional criteria All mandatory criteria...

Ngày tải lên: 21/06/2014, 03:20

... synthesis and purification were purchased from Roth, Germany The solvents were of reagent grade and used without further purification Amino acids, derivatives and coupling agents were purchased from ... of structural and electronic properties of fullerene and metallofullerenes by surface chemical modifications J Nanosci Nanotechnol 2007; 7: 1085-101 Ito Y, Fujita W, Okazaki T, et al Magnetic properties ... intensity-modulated radiation therapy (IMRT) and the use of heavy ions demands absolute reliability of new diagnostics and treatment planning for prostate and brain tumors By the fact that the rare...

Ngày tải lên: 26/10/2012, 09:07

... • Exercise key words; standard responses • Key words and concepts - creative and recall • Memory - a comparison between standard note and key word noting • Transition from advanced key word note ... concentration and memory) and then branch out in an organised fashion around that image, using Key-Words and Key Images As you continue to build up the Mind Map, your brain creates an organised and integrated ... for himself, and to explain the course to his wife, children and business colleagues The central image refers to the integration of the brain and the body The branches, clockwise from 'exercises'...

Ngày tải lên: 09/08/2013, 11:51