real time pcr for the detection of pathogens in meat

... MINISTRY OF EDUCATION AND TRAINING NONG LAM UNIVERSITY - HO CHI MINH CITY Faculty of Chemical Engineering in Food Technology REAL-TIME PCR FOR THE DETECTION OF SALMONELLA IN FOOD Instructor: ... assay”) measures the amount of accumulated PCR product at the end of the PCR cycle Diagnostic real-time PCR for the specific detection of Salmonella in foods ¡is increasingly being used as a rapid ... to PCR, real-time PCR is also a DNA cloning technique based on thermal cycles Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in

Ngày tải lên: 31/07/2024, 17:15

... anti-platelet therapy The real-time PCR method was developed for the identification of the specific mutations in the CYP2C19 gene (CYP2C19 *2 and CYP2C19*3) and ITGB3 gene (PlA1/ A2) The real-time PCR ... and increased platelet activity, as determined by platelet aggregation and/or fibrinogen binding [7 10] The Realtime - PCR method is an accurate way of defining polymorphism, with the advantage of ... possibilities for applying this technique as a routine test before indications of clopidogrel and aspirin therapy for PCI patients The aim of this study was to investigate the frequencies of CYP2C19

Ngày tải lên: 22/01/2020, 14:38

... the limit of detection for the cobas platform The percentage of mu-tant alleles in the blended samples was verified by MPP Potential interfering substances The effects on the performance of the ... oncologist, relying on information from the molecular pathology of the tumor tissue [11] In recognition of the need for accur-ate testing, these organizations have started consolidating guidelines for molecular ... NCT00446225) Though there has been consideration of the use of next gener-ation sequencing in routine clinical diagnostics, for the accurate selection of patient therapy, method of testing for EGFR mutations

Ngày tải lên: 05/11/2020, 06:20

... contains all strains of a species in the training set Below the horizontal line, we show results for the combination of methods with the consensus filter In these cases, the performance is given for ... reflects the larger bias towards HPs in the training set Overall, the effect of the larger database is small We further assessed the performance of all approaches in a ROC analysis Here, instead of the ... Illumina reads To that end we used the Mason read simulator with the default Illumina error model48 The number of reads sampled per genome differed for the training and test sets In training binary

Ngày tải lên: 04/12/2022, 15:55

... licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central ... mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office ... guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination 3 Terms and definitions For the purposes of this International Standard, the

Ngày tải lên: 07/03/2014, 16:20

... cells in the microcavity allows quantification of the fluorescence intensity and thus the inflammatory status of single cells The determination of the effect of miniaturization on nanoparticle-induced ... is of special interest, since one of the main functions of the immune system is to deal with foreign materials [44] In order to determine whether or not the MCC method could be suitable for the ... hMSCs, also in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies

Ngày tải lên: 21/06/2014, 00:20

... (kT) The performance of the previous detectors are computed in terms of Probability of Detection (PD) of the known useful signal as a function of either its input SNR or the Probability of False ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... interference increases Again, the O1 detector gives the best performance while the O9 detector gives the lowest ones Again, the a priori knowledge of the noise statistics or of a TNAR or of the

Ngày tải lên: 21/06/2014, 00:20

... derived. The optimal operating point of the linear classifier is specified with and without reject option. The contribution of this paper is in the assessment of the impact of reject option in the ROC ... . The initial estimates of t and ϑ can be corrected, when the operating point they define lies inside the convex hull of the ROC curve with rejection. Since the probability of false alarm and the ... rejection at the optimal operating point. The values of t and ϑ of the aforementioned optimal operating point are better estimates than the initial ones. If the initial estimates of t and ϑ define

Ngày tải lên: 21/06/2014, 20:20

... rates of 2D in 3D defects up to 10% are allowed Those conditions define the inspection constraints of the whole inspection system as well as of every element of the processing chain The primary information ... illumination for the inspection of optical lenses When the variations of the surface to be inspected are more pronounced, an adapted geometry of the illumination facilitates the recording of the ... dP,px (in pixel) in the u direction of the image The ratio of dP,px with the period dP,mm (in millimeter) of the pattern LPreflected gives the image resolution in u direction of the image coordinate

Ngày tải lên: 22/06/2014, 19:20

... histogram, all of which is used to generate indexes of abnormality The resulting patterns of the indexes are then compared for abnormality to the patterns in the reference database to reach a final diagnostic ... monitoring of CAD to determine the level of disease or quantifying the patient’s myocardial ischemic burden at the time of the testing Resting ECG analysis, including that of the 12-lead ECG, typically ... stenosis, the area under the ROC curve was calculated to be 0.843 [0.802-0.884] The coordinates of the curve indicated that the cut-off of 4.0 (as pre-defined by the manufacturer) provided the best

Ngày tải lên: 08/08/2014, 16:23

... histogram, all of which is used to Int. J. Med. Sci. 2008, 5 52 generate indexes of abnormality. The resulting patterns of the indexes are then compared for abnormality to the patterns in the reference ... segment of the ECG data, the waveform strays from the baseline by ≥ 3 mm, • a radical deviation away from the baseline 80° of ≥ 2 mm from the baseline, occurring two or more times, • a single ... against the final medical diagnosis of at least two independent expert diagnosticians in the field, including results of angiography and enzyme tests. The current diagnostic capability for

Ngày tải lên: 08/08/2014, 17:20

... to the limited reliability of indirect immuno- fluorescence (IIF) assays for the detection of these aab, the lack of access to international reference serum samples, and the misunderstanding of ... highest prevalence of other concurrent aab in the Rib-P Figure 1 Indirect immunofluorescence staining pattern of anti-Rib-P-positive samplesIndirect immunofluorescence staining pattern of anti-Rib-P-positive ... for further analysis compared with the routine cohort of 345 samples. In the initial cohort, staining patterns were read in the routine laboratory without special attention to the CSP. In con-

Ngày tải lên: 09/08/2014, 13:22

... emphasized These curves are nearly linear for power under 70% and, in this linear part, the slope (ie the gain in power Trang 9per extra individual measured) increases with a The resulting increase in ... of dominance, before the experiment is designed, then maximum power will be attained by limiting the segregating subpopulation to the single backcross showing segregation However, the power of ... effect (k = 1 for AA, 2 for AB and 3 for BB) and pi is the probability of the kth genotype in the ith genetic type. Under the preceding fixed alleles hypothesis: The case where the within-major-genotype

Ngày tải lên: 09/08/2014, 18:21

... in the region of the N-term- inal domain of t he glycogen branching enzyme family, reported to be essential for the size of the glucan chains transferred and also for the catalytic activity of ... quency of the wheat population used in the present work did not require deep pooling to increase the throughput of the screening. Our finding of a mutation density of 1 SNP for each 40 kb is in agreeme ... HRM instrument allows the monitor- ing of fluorescence changes in real time as the tem pera- ture of the samples is slowly increased. While detection of SNPs in homoduplex DNA is possible, insta

Ngày tải lên: 11/08/2014, 11:21

... view of the extent and significance of LD in the cattle populations using the Haploview 4.0 program Numbers in the blocks indicate the percentage of the LD metric D’ values > 0.3; shadings indicate ... lnRθ of several linked loci for the inference of selection While the single-locus lnRθ-test is largely independent of the demographical past, the additional power of linked loci is balanced by the ... lines indicate the critical cutoff for the P-value at the 0.05 level. Trang 9comparisons for each of the three different measures oflnRθ (Table 2) Of the pairwise comparisons, a total of 28 and

Ngày tải lên: 14/08/2014, 13:21

... mainly in muscles Nowadays, there are no definitive guidelines available to approve usage of DNA vaccines in food- producing animals In this work, the QRTPCR method was used for the study of the ... plasmid The methodology was initially investigated for sensitivity, spe-cificity and linearity, in the detection of pDNAX plasmid Firstly, the detection method was studied as part of the protocol for ... frozen in liquid nitrogen and stored at -70°C until further processing Experiment II The influence of the age of the mice on the dynamics of plasmid clearance during 1 month period after adminis-tration

Ngày tải lên: 14/08/2014, 19:22

... Temperature distribution of the two fluids flowing through the inner and outer jacket. T1,in and T2,in are the temperature of fluid in the input end of the inner jacket and the outer jacket, respectively. ... not be formed 16 . This also has the effect of decreasing the melting temperature of DNA. Therefore, the melting temperatures of M2, M60, M69 were lowered compared to those in the absence of urea8, ... like the integration of affinity trapping and impedance detection2 4, the integration of mechanical trapping, cell lysis and µPCR in a weir-type filter chip31, the integration of DEP

Ngày tải lên: 16/09/2015, 15:54

... mainly rely on the biocatalytic reaction involving the reduction of flavin adenine dinucleotide (FAD) in the glucose oxidase (GOx) by glucose, followed by the reoxidation of the reduced form of ... in biosensors is available.7 On the other hand, the role of the transducer is to transform the biorecognition event resulting from the interaction of the analyte with the biological element into ... acknowledged all the sources of information which have been used in the thesis This thesis has also not been submitted for any degree in any university previously The content of the thesis has been

Ngày tải lên: 22/09/2015, 15:18

... nested PCR for the detection of IMNV 22 Table 4.2: PCR reagents of nested PCR for the detection of IMNV (change in Taq polymerase concentration) 23 Table 4.3: Optimization of RT-PCR assay for the ... conducted two following contents:  Optimization of chemical ingredients of RT-PCR for the detection of IMNV  Optimization of cycling conditions of RT-PCR for the detection of IMNV Trang 12CHAPTER ... ORF1locates RNA-binding proteins and contains s dsRNA-bingding motif in the first 60 amino acids A capsid protein was encoded in the second half of ORF1 and determined by amino acid sequencing The ORF2

Ngày tải lên: 22/09/2015, 17:04

... rates) of the two psychometric scales for detecting MDD and PTSD This will involve the exploration of the sensitivity and specificity of various cut-off points for these two scales, and whether ... cut-off scores for DASS-21 For the valid detection of probable MDD, and using the decision rule discussed in the Method, the following is recommended: (i) the DASS-21 total cut-off score of 30 can ... well as payments for pain and suffering Claimants must have reported the accident and injuries within 48 h of the road crash, and lodge the CTP claim within months from the date of the crash This

Ngày tải lên: 10/01/2020, 14:22