production of foreign proteins using hairy roots

... patterns in the analysis of antibody titer by ELISA using IROMP and OMP of P multocida A:3 (Fig and 6) However, the response in group was lower than in the other groups in case of using IROMP as a coating ... toxicity of P multocida A:3 in hens, as indicated by a reduction of egg production and increases with mortality Survival rates of experimental groups (1 to 4) injected with the causative agents of ... immunized with various antigens of B bronchiseptica and P multocida D:4, as shown in Table Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica,...

Ngày tải lên: 07/08/2014, 15:20

... expression patterns (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR (B) The amount of p19 gag in the pooled concentrated ... molecular clones with deletions of the 3' LTR of different lengths and the corresponding viral expression patterns Various Various molecular clones with deletions of the 3' LTR of different lengths and ... downstream from the beginning of the 3' LTR, which constitutes the C-terminal portion of the tax downstream sequence, might be involved in the inhibition of the replication of HTLV-1 genes in infectious...

Ngày tải lên: 12/08/2014, 04:21

... produces 10,000 L of milk per year compared to 6000 L of urine One of the negative points in production of proteins by transgenic animals is the length of time needed to assess production level ... placed under the strong control of the viral polyhedrin promoter, allowing expression of heterologous protein of up to 30% of cell protein Production of recombinant proteins in the baculovirus expression ... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of nonglycosylated proteins...

Ngày tải lên: 08/09/2015, 22:11

... produces 10,000 L of milk per year compared to 6000 L of urine One of the negative points in production of proteins by transgenic animals is the length of time needed to assess production level ... placed under the strong control of the viral polyhedrin promoter, allowing expression of heterologous protein of up to 30% of cell protein Production of recombinant proteins in the baculovirus expression ... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of non-glycosylated proteins...

Ngày tải lên: 09/09/2015, 12:14

... produces 10,000 L of milk per year compared to 6000 L of urine One of the negative points in production of proteins by transgenic animals is the length of time needed to assess production level ... placed under the strong control of the viral polyhedrin promoter, allowing expression of heterologous protein of up to 30% of cell protein Production of recombinant proteins in the baculovirus expression ... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of non-glycosylated proteins...

Ngày tải lên: 09/09/2015, 12:34

... with foreigners, to interview and from that; we can learn something different from last assignments Because of the purpose of the assignment, is that encouraging students to meet and talk with foreigners, ... Interview Question • First half of the result part: from background to the sources of information and how useful/accurate it was Nguyen Thi Hong Nam: • Second part of the result: From accommodation ... by: Do you often use a system of tourism information when you travel in Vietnam? 10.Is it easy for you to approach the tourism information center? Give me your trouble if no? 11.Do you often get...

Ngày tải lên: 25/04/2013, 15:42

... atop of the electrolyser 2.5 Performance of the membrane electrode assembly (MEA) The performance of prepared MEA is evaluated using in-house fabricated single cell assembly A DC voltage of 1.8 ... has been made to evaluate the performance of composite membrane in water electrolysis process for the production of hydrogen The characterization studies of the Composite membrane are done by ion ... concentration of the titrant, Wsample is the dry mass of the sample (g) 2.7 Hydrogen production The hydrogen produced during the electrolysis operation is collected in vessels provided at the top of the...

Ngày tải lên: 05/09/2013, 16:11

Ngày tải lên: 09/03/2014, 14:20

... terms of the proper assignment of words to part -of- speech classes However, it must be capable of separating identically spelled words into different classes on the basis of function These words often ... distinctions, the current version of the parser fails to supply this information A new version of UTTER should include a better parser which is capable of making these sorts of part -of- speech dlstlnctlons ... SYSTEM ORGANIZATION The current implementation of UTTER operates in one of three modes, each of which corresponds to one of the three tasks required of the system: (I) (2) trainin~ mode: flagglr~...

Ngày tải lên: 24/03/2014, 05:21

... The effect of different solid substrates on pectinase production was studied by using a variety of solid substrates (wheat bran, rice bran, apple pomace) The original moisture content of the apple ... after 48 h of incubation at 37 °C A decline in pectinase activity in wheat bran (26%) and rice bran (8.2%) was observed after 72 h of incubation Moreover, the production of this enzyme using SSF ... pomace was 80% Effect of moisture contents on pectinase production using wheat bran and rice bran was studied by adding different amount (50%, 60%, 66.7%, 75%, 77.8%, and 80%) of distilled water...

Ngày tải lên: 01/04/2014, 00:21

... Patterns of EIAV Are Closer to Those of Influenza than of disorder patterns of HIV/SIV Analysis of Figure reveals that the pattern of the predicted disorder in EIAV matrix protein is closer to that of ... behavior of the matrix proteins [3] Conclusion Matrix Disorder of Retroviruses Varies with Nature of the Virus A peculiar finding of this paper is the pattern of predicted disorder of EIAV p15 ... Figure PONDR/B-factor plots of matrix proteins of various viruses A) the influenza A M1 protein B) the SIVmac P17 protein PONDR/B-factor plots of matrix proteins of various viruses A) the influenza...

Ngày tải lên: 20/06/2014, 01:20

... (shaking speed of 300 rpm in the presence of 12.5 g l-1 of YE and 15 g l-1 of AS) After a short Lag phase of about h, we witness an exponential phase of bacterial growth and maximum number of viable ... the production of the Bacillus sp MD2 phytase at 327 U ml-1 by fed-batch cultivation, the majority of earlier attempts with expression of Bacillus phytases in Escherichia coli have resulted in production ... as the amount of enzyme capable of releasing µmol of inorganic phosphate (Pi) min-1 (from phytic acid) under the optimal conditions Identification of critical culture variables using Plackett–Burman...

Ngày tải lên: 20/06/2014, 20:20

... the proteins and the active sites of nanoholes Third, the transduction system was modified for application to a chip-based detection method This would premit the fabrication of materials using off-theshelf ... absorption peak as a function of the volumetric ratio of SOD1 formulations As the ratio of SOD1 Figure Changes in the maximum absorbance peak as a function of the volumetric ratio of a mixed SOD1formulation ... SPR and AFM study of the effect of surface heterogeneity on adsorption of proteins J Chem Phys 2004, 121:2264 33 Kim H, Cho IH, Park JH, Kim S, Paek SH, Noh J, Lee H: Analysis of a nonlabeling...

Ngày tải lên: 21/06/2014, 04:20

... Hydrolysis Profile Figure shows a typical hydrolysis profile of palm oil at temperature of 45ºC for an initial palm oil concentration of 0.1 g palm oil/ml iso-octane at agitation speed of 200 rpm ... indicator The degree of hydrolysis, X is calculated as below: X,% = (ml NaOH used) (molarity of NaOH) (average molecular weight of fatty acid) 10 (weight of sample) (1) The rate of hydrolysis, r ... every 30 from each flask 2.3 Determination of Degree of Hydrolysis (Conversion) and Rate of Hydrolysis The degree of hydrolysis was determined by titration of the oil phase samples with 0.1 M sodium...

Ngày tải lên: 07/08/2014, 14:20

... cells with electrofusion may be more efficient for the production of large numbers of iSCNT whale embryos Despite the relatively normal morphology of the iSCNT whale embryos, all of the cleaved ... on Day of IVC were examined, most of the embryos exhibited nuclei with metaphase-like structures (Figs 2C and D) Irrespective of the type of macromolecule added to the culture medium, all of the ... cultured in the presence of FWS In comparison, no blastocysts developed in the presence of FBS or FWS When the nuclear morphology of iSCNT Effect of a two-step culture method using different macromolecules...

Ngày tải lên: 07/08/2014, 23:22

... concentration of 10 mM to measure the joining activities of these two proteins Results The pH-dependence of both the processing and the joining reactions with ASV and HIV-1 IN proteins in the presence of ... development of a number of novel metal chelating inhibitors of HIV-1 IN, several of which were found to be effective in blocking both processing and joining in the presence of either Mn++ or Mg++ Of special ... coefficient of 2–2.5) than Mg++ (0.6– 1.3) This is indicative of a greater cooperativity of inhibitor binding with the Mn++ cofactor, and is consistent with results from previous studies of this class of...

Ngày tải lên: 10/08/2014, 05:21

... Western blot analysis of cell extracts with antibody to GFP showed that the abundance of all three fusion proteins diminished in the presence of CHX (Figure 4a) The half-life of the proteins determined ... Two of the proteins showed a half-life of about hour, while the proto-oncogene protein appears to initiate abrupt degradation within about hours of treatment with CHX The results for all three proteins ... intensity of R2 was too low to detect after CHX treatment The lack of susceptibility of the brighter R5-R6 subpopulations was most likely the result of their expressing predominantly stable proteins, ...

Ngày tải lên: 14/08/2014, 14:21