process analysis and system implementation

Construction of bacterial artificial chromosome library for kineosphaera limosa strain lpha5t and screening of genes involved in polyhydroxyalkanoate synthesis

Construction of bacterial artificial chromosome library for kineosphaera limosa strain lpha5t and screening of genes involved in polyhydroxyalkanoate synthesis

... father, Ji Lieshun, mother Li XueQin and my son Liu SiBo, sisters Ji ZhiPing, Ji ZhiJing, and my deceased husband Liu ZhiSheng Without their love, understanding and constant support through all these ... putative PAO and ‘GBacteria’/GAO (Seviour et al., 2003) To better understand the EBPR processes, it is necessary to understand the metabolisms of the three key biopolymers (PHA, glycogen and polyP) ... receiving water bodies, and the P-accumulating microorganisms are either returned to the process or disposed as waste An EBPR process includes an anaerobic followed by an aerobic stage and a settling...

Ngày tải lên: 03/10/2015, 21:58

116 493 0
The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals

The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals

... expression levels and flow cytometric analysis 101 3.2.1b Transcriptional and genomic analysis 105 3.2.2 Recovery of CaSki ATCC cells treated with 5AzadC 108 3.2.2a Protein expression levels and flow ... chromosome inactivation, genomic imprinting and suppression of foreign and parasitic DNA (Baylin and Herman, 2000; Jones and Laird, 1999; Robertson and Wolffe, 2000) DNA methylation is also one ... E2, E3, E4, E5, E6 and E7) and two late genes (L1 and L2) The upstream regulatory region (URR) or long control region (LCR) is located between the L1 and E6 ORFs The size and location of the...

Ngày tải lên: 16/09/2015, 15:55

282 281 0
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

... and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and the nucleotide positions 16024±16 324 and ... relationship between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 ... activity and expression of base excision repair and mismatch repair are down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA...

Ngày tải lên: 25/10/2012, 11:18

12 557 2
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"

Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"

... amino acid transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and high output production ... incidents involving anesthesia To understand the role of citrulline-NO cycle enzymes, GS and the oxidative status in anoxia and reperfusion, NOS, AS, AL, GS and arginase activities along with the ... were removed and killed by decapitation The brains were quickly removed and the different regions (CC, CB and BS) were separated according to the procedure described by Sadasivudu and Lajtha (22)...

Ngày tải lên: 26/10/2012, 09:07

8 623 0
Tài liệu .An ARCO Book ARCO is a registered trademark of Thomson Learning, Inc., and is used herein under pptx

Tài liệu .An ARCO Book ARCO is a registered trademark of Thomson Learning, Inc., and is used herein under pptx

... GMAT stand for Graduate Management Admission Test, which is a standardized exam given at various locations in the United States and Canada and around the world Throughout North America and in ... 376 Answer Key and Explanations 378 Roots and Radicals 379 Exercise 381 Answer Key and Explanations 382 Factoring and Algebraic Fractions ... test preparation, and financial aid Its Web site offers searchable databases and interactive tools for contacting educational institutions, online practice tests and instruction, and planning tools...

Ngày tải lên: 20/01/2014, 20:20

696 1K 1
Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot

Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot

... applications, including starch processing, cellulose degradation and ethanol production, pulp bleaching, leather and textile processing, chemical synthesis, food processing, and the production of detergents, ... this approach would obviously avoid the expensive and laborious enzyme engineering process, and revolutionize industrial and biotechnological processes Obviously, this approach relies on the availability ... provide a system that is able to operate under temperatures higher than those currently employed in systems based on mesophilic and thermophilic biocatalysts Hyperthermostability: genomic and proteomic...

Ngày tải lên: 07/03/2014, 05:20

13 469 1
Báo cáo khoa học: "Case markers and Morphology: Addressing the crux of the fluency problem in English-Hindi SMT" pot

Báo cáo khoa học: "Case markers and Morphology: Addressing the crux of the fluency problem in English-Hindi SMT" pot

... the lemma and suffix factors on the source side, and the lemma and suffix/case marker on the target side The translation steps are i) lemma to lemma and ii) suffix to suffix/case marker, and the generation ... to medium length and long sentences Reorder: Ú я gaandhii darshana va gaandhii raashtriiya sangrahaalaya raajaghaata men hai Semantic: Conclusion Ú я gaandhii darshana va gaandhii raashtriiya ... prepositions, and clausal complements, modifier relations like adjectival, adverbial, participial, and infinitival modifiers, and other relations like coordination, conjunct, expletive, and punctuation...

Ngày tải lên: 08/03/2014, 00:20

9 466 0
DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

... B, and C) and nuclei in the intestine of an early L4 larva (A’, B’, and C’) are indicated by arrows Panels show DNA staining by DAPI (A and A’), EdU staining (B and B’) and merged images (C and ... diagnostic and prognostic marker in many neoplasms has been extensively studied and its role in standard biological evaluation of the clinical course and management of cancers among them Lymphomas and ... 3,4,5,6,7 specially in preneoplastic and cancers and also in some viral and endocrine diseases eg Diabetes They have been suggested as standard diagnostic and prognostic biomarkers in some tumoral...

Ngày tải lên: 08/03/2014, 19:20

312 520 0
Lecture 3 DNA RNA and protein synthesis great

Lecture 3 DNA RNA and protein synthesis great

... a sugar, and a nitrogen base  DNA is in a double strand  The nitrogen bases have compliment partners  Adenine-Thymine  Cytosine-Guanine Just a note about RNARNA is single-stranded and acts ... subunits}   WHAT DOES “DNA” STAND FOR? DNA’s proper name isDeoxyribonucleic acid!  Consists of a ribose SUGAR with a “missing oxygen” (that’s the de-oxy part)  And it’s found in the nucleus ... ribosomes  DNA and protein synthesis, then, happens this way:  DNA sequence codes (how letters are put together) produce messenger RNA sequence codes  The messenger RNA leaves the nucleus and attaches...

Ngày tải lên: 13/03/2014, 16:36

17 674 2
DNA, RNA and proteins

DNA, RNA and proteins

... cellular functions • Proteins everything, and DNA gets all the credit! • Think of them as tiny minions who all the work DNA and Protein Synthesis DNA and Protein Synthesis • Codon: three bases ... ribosomes (rRNA) in the cytoplasm DNA and Protein Synthesis – Codons and amino acids Name the Amino Acid: UAU Tyrosine CCG Proline AGU Serine GCA Alanine DNA and Protein Synthesis But… How does ... the ribosome releases the protein chain DNA and Protein Synthesis - Summary DNA and Protein Synthesis Practice making mRNA using the DNA template DNA and Protein Synthesis • Amino acids are linked...

Ngày tải lên: 13/03/2014, 16:38

18 1K 1
Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx

Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx

... with water and that have low polar characteristics (hexane, diisopropyl ether, and 3-pentanone), and those that have polar properties and are water miscible (ethanol and acetonitrile) System setup ... acetonitrile with 25% water and (F) in the fully hydrated system The molecular surface corresponds to the average structure of cutinase from the 3–7 ns period, for each solvation system and for all replicas ... contours at percentiles approximately between 93% and 98% for hexane, diisopropyl ether, and 3-pentanone, and at 99% for ethanol, acetonitrile, and water, giving the clearest picture for the preferential...

Ngày tải lên: 16/03/2014, 10:20

13 435 0
Biomass for renewable energy, fuels and chemicals

Biomass for renewable energy, fuels and chemicals

... suitable for grazing and browsing Other land areas include crop and pasture land and farmsteads, strip mines, permanent ice and snow, and land that does not fit any other land cover This a p p ... 106 Percent Nonfederal land Forest Rangeland Other land Transition land Total 179.41 178.66 279.09 14.41 651.57 18.8 18.7 29.2 1.5 68.2 Federal land Forest Rangeland Other land Total 102.14 133.10 ... Water Inland water Other water Total Grand total land and water 24.75 19.28 44.03 956.54 2.6 2.0 4.6 aAdapted from USDA Forest Service (1989) The data for forest, rangeland, and other land are...

Ngày tải lên: 16/03/2014, 18:10

649 537 0
Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

... both RNA and DNA dependent; and (b) RNase H activity that selectively hydrolyzes the RNA strand of the RNA:DNA hybrid formed during synthesis of the minus () strand DNA that uses (+)-strand RNA ... interactions Analysis of the trajectories was performed with Desmond simulation analysis event and VMD [48] Y181C and K103N mutant enzymes The wild-type RT residue 181 was mutated to Cys and the 103 ... using the tC5U p12 hybrid as the substrate Reactions and PAGE analysis were carried out as described in Materials and methods Four major bands were resolved as reaction products, each from a...

Ngày tải lên: 22/03/2014, 16:20

14 426 0
Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc

Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc

... interactions with the O6 and N7 atoms of G13 (complementary to the conserved C8 base on the sense strand) and the N7 atom of A12 (complementary to the conserved T9 base on the sense strand [17]) The M ... Phosphorylation of WT and K182G mutant DevR proteins with DevS201P (phosphorylated cytoplasmic domain of DevS) Lane 1, DevSP; lane 2, DevSP and DevR K182G mutant protein; and lane 3, DevSP and WT DevR ... residue in each subunit is involved in H-bonding with O6 and N7 atoms of G13 and with the N7 atom of A12 in the DNA strand In silico analysis of WT versus K182 mutant DevR protein reveals the...

Ngày tải lên: 22/03/2014, 16:20

9 352 0
Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

... (s-NAP; lanes c and c¢), medium-size NAP (m-NAP; lanes d and d¢) or largesize NAP (l-NAP; lanes e and e¢) and exposed to DNase I Controls were the whole genomic DNA (lane a) and the DNA fully ... & Thomas TJ (1999) Ionic and structural specificity effects of natural and synthetic polyamines on the aggregation and resolubilization of single-, doubleand triple-stranded DNA Biochemistry 38, ... (lanes c, c¢ and c¢¢), spermidine (lanes d, d¢ and d¢¢) or putrescine (lanes e, e¢ and e¢¢) and then exposed to DNase I Whole genomic DNA (lane a) and DNase I-digested genomic DNA (lane b) were...

Ngày tải lên: 23/03/2014, 15:20

11 383 0
Methylation - From DNA, RNA and Histones to Diseases and Treatment by Anica Dricu ppt

Methylation - From DNA, RNA and Histones to Diseases and Treatment by Anica Dricu ppt

... consists of β-strands and is flanked by α-helices from each side Six of seven β-strands have a parallel orientation, while the 7th β-strand is located between the 5th and the 6th β-strands in an antiparallel ... distribution, and reproduction in any medium, provided the original work is properly cited 4 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones to Diseases and ... promoters of indu‐ Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones to Diseases and Treatment cible genes and interacting with transcription initiation...

Ngày tải lên: 24/03/2014, 02:21

310 344 1
Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx

Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx

... 20-mer dsDNA and PsSiR as a competitor, and then the mixture was electrophoresed (Fig 6A) The densities of shifted band signals at two-, five- and 10-fold excess of competitor were 34, 17 and 12%, ... SiR activity and DNA-binding activity We also examined differences in the properties of PsSiR and ZmSiR, such as DNA binding and localization within chloroplasts Results Isolation and molecular ... active site of the enzyme through four cysteine ligands, Cys434, Cys440, Cys479, and Cys483, and that four basic residues, Arg83, Arg153, Lys215, and Lys217, are involved in the substrate coordination...

Ngày tải lên: 30/03/2014, 08:20

16 329 0
Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

... serum and phenol red were washed with NaCl ⁄ Pi, and the DMEM without fetal calf serum and phenol red was added (2 mL per 60-mm dish) followed by the addition of CSE (2% final concentration) and ... methyltransferase Suv39 h [33] and mouse cells deficient in the ribonuclease Dicer [34], which is involved in processing and formation of siRNA We compared cDNAs synthesized using random primer on total ... using two different dilutions (1 : and : 729) of template cDNA from the control clone (lanes and 3) and the same dilutions of cDNA from the KD-1 clone (lanes and 4) Control reactions with b-actin...

Ngày tải lên: 30/03/2014, 11:20

11 463 0
Báo cáo khoa học: "Local constraints on sentence markers and focus in Somali" doc

Báo cáo khoa học: "Local constraints on sentence markers and focus in Somali" doc

... Ramsay and H Mansour 2003 Arabic morphosyntax for text-to-speech In Recent advance in natural language processing, Sofia A M Ramsay and R Sch¨ ler 1997 Case and word ora der in English and German ... discourse reasons, and here the +fullForm version is used ((Lecarme, 1995) uses the terms ‘restrictive’ and ‘extensive’ for -fullForm and +fullForm respectively) ‘kau’ to ‘ku’ and ‘kiiu’ to ‘kii’ ... an argument) and a set of inflectional affixes which mark aspect, tense and agreement (Andrzejewski, 1968) The forms of the tense and agreement markers vary depending on...

Ngày tải lên: 31/03/2014, 01:20

8 366 0
Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc

Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc

... single band, at 61 kDa (SASIG2) and 65 kDa (SASIG3), respectively, both in the whole-cell (lanes and 6) and chloroplast fractions (lanes and 7) It was therefore concluded that both SASIG2 and SASIG3 ... Western analysis, protein samples were separated by SDS/PAGE and transferred onto nitrocellulose membrane Proteins were incubated with anti-SASIG2 and anti-SASIG3 antisera, respectively, and detected ... each immunoblotted using antisera against SASIG2 (lanes and 4) and SASIG3 (lanes and 7), or the corresponding preimmune sera (pi) (lanes and 5), respectively Lane shows the Coomassie-stained chloroplast...

Ngày tải lên: 31/03/2014, 07:20

13 304 0
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