plant tissue culture practice

... applications of plant tissue culture is large-scale plant multiplication for the production of insect/disease/virus free plants, particularly valuable for vegetatively propagated plants such as ... approximately 65% of total electricity in tissue culture labs [6] In most plant tissue culture facilities fluorescent lights are fixed on the shelves of culture racks at a particular height, and ... annua plants (Figs. 4 5) In general, plants cultured under red/blue light at a ratio of 3:1 per-formed the best (Figs. 4 5) Compared to tobacco plants cultured under full spectrum white light, plants

Ngày tải lên: 19/11/2022, 11:41

... KHOA HỌC ỨNG DỤNG BÁO CÁO HỌC KỲ MÔN: CÔNG NGHỆ SINH HỌC THỰC VẬT ĐỀ TÀI: VIRUS-FREE-PLANT TISSUE CULTURE THÀNH PHỐ HỒ CHÍ MINH, THÁNG 3 NĂM 2022 Trang 2MỤC LỤC I Mở đầu 3 II Nuôi cấy ... cây sạch bệnh virus in vitro 1 Các cách nuôi cấy meristem - Nuôi cấ đỉnh phân sinh (meristem-tip culture)  Tách chính xác meristem ó kí h thước < 0,3mm  Nuôi cấ tr n m i trường dinh dưỡng

Ngày tải lên: 21/03/2023, 21:41

... (1978) The impact of plant tissue culture on agriculture In T A Thorpe (Ed.), Frontiers of plant tissue culture 1978 (pp 15–26, 518–524) International Association of Plant Tissue Culture: Univ of ... (1991) Plant cell and tissue culture London: Open Univ Press Street, H E (Ed.), (1973) Plant tissue and cell culture Berkeley: Univ of CA Press. Street, H E (Ed.), (1974) Tissue culture and plant ... Biology, 86, 109–127. Trang 2922 Plant Tissue CultureZenk, M H (1978) The impact of plant cell culture on industry In T A Thorpe (Ed.), Frontiers of plant tissue culture 1978 (pp 1–13) Univ of

Ngày tải lên: 04/10/2023, 15:52

... new plantlets or microplants (Sub-culturing) The new plants can then be placed in soil and grown in the normal manner Organization of tissue culture laboratory A sophisticated plant tissue culture ... room; Culture/growth room Plant Tissue Culture Media Composition One of the most important factors governing the growth and morphogenesis of plant tissues in culture is the composition of the culture ... fast development and implementation of biotechnology in agriculture has been provided by achievements in plant tissue culture Plant tissue culture represents the most promising areas of application

Ngày tải lên: 20/09/2020, 20:08

... anthers…), tissues and cells Types of culture include: meristem culture, callus culture, cell culture (single cell, protoplast), embryonic culture, anther culture, pollen culture, thin cell layer culture ... to domestic science and technology, plant tissue and cell culture in Vietnam has also made impressive imprints in the development of plant tissue and cell culture in the world In this review, ... Keywords: Achievement, contribution, development, plant tissue and cell culture, Vietnam Citation: Nguyen Duc Thanh, 2020 Plant tissue and cell culture in Vietnam: forty-five years of development

Ngày tải lên: 27/09/2020, 13:36

... sow 3 or 4 Transferring plantlets for further growth Pinguicula heterophylla in culture tranferring plantlets plants in-vitro and also planted out I have kept my cultures under coolish home ... state for home tissue culture, Part I, Brian Johnson, CPS Journal 14, 18-19 2 Achieving the sterile state for home tissue culture, Part II, Brian Johnson, CPS Journal 16, 9-10 3 Tissue culture of ... 1722101-0850 ISBN 0-947946-22-5 paperback 18 Methods in Plant Tissue Culture by Paul J Bottino, 1981, 72 pages 19 Experiments in Plant Tissue Culture by John H Dodds and Lorin W Roberts, 1993 (2nd

Ngày tải lên: 20/04/2014, 16:35

... formation of new shoots) and were transplanted in the nursery. Propagation by tissue culture Plant material For initiation of Q robur and Q petraea organ cultures, explants were taken from shoots of ... embryos cultured on these media produced embryo-genic tissue within 7-9 weeks (fig 2) The embryogenic competence was maintained by embryogenic tissue subculture Em-bryogenic tissues cultured ... initial explants for the es-tablishment of adult tree cultures (stump sprouts of 12 40-yr-old trees were used). The explants of adult trees were grown on the same media as seedling cultures Ex-plants

Ngày tải lên: 08/08/2014, 19:21

... phenome- non of somaclonal variation in plant tissue cultures is the results of concerted action of both genetic and epigenetic instabilities induced by the tissue culture process [47], and activity ... profiles showing the tissue culture-induced mPing activity in the three rice ssp. indica cul-tivarsFigure 1 Examples of transposon display (TD) profiles showing the tissue culture-induced mPing ... perturbation in the epigenetic modifica- tion by certain stress conditions like tissue culture. Results Tissue culture-induced mPing transposition Transposon display (TD) analysis was performed

Ngày tải lên: 12/08/2014, 03:20

... Animal Tissue culture Trang 2What is tissue culture? In vitro culture (maintain and/or proliferate) of cells, tissues or organs  Types of tissue culture • Cell culture Trang 3Cell culture Cell culture ... of tissue culture Explant: living cells, tissues, or organs from animals or plants that transfer to a nutrient medium. Trang 4Cell culture & Enzymatic DissociationTissue from an explant ... culture11 Tissue Primary culture Cell line Continuous cell line Subculture Finite numbers Indefinite numbers Trang 12Types of Cell culture1 Primary Cultures  Derived directly from excised tissue

Ngày tải lên: 18/05/2017, 07:40

... diverse coconut explanted tissues, the slow growth of these explanted tis-sues in vitro, and their further lack of vigour when planted ex vitro (Fernando et al.2010) Nonetheless, tissue culture and ... achievements in coconut tissue culture have not been attained as rapidly as they have for many other plant species (Fig.1) Some of the reasons often cited for the slow advancement in tissue culture include ... for embryo germination and plantlet 1954 First coconut tissue culture attempt using zygotic embryos (Cutter and Wilson 1954) 1964 First plant back from zygotic embryo culture (De Guzman and Del

Ngày tải lên: 17/12/2017, 15:22

... such plant-based culture media Cell growth and biomass production in liquid batch cultures were comparable to those reported with the synthetic culture media In addition, the tested plant-based culture ... recovered with plant-based culture media, being dis-tinct from that obtained with the chemically-synthetic culture media Proteobacteria were the dominant (78.8%) on plant-based agar culture medium ... cides)[32] Cultivability and recovery of rhizobacteria associated with plant roots on plant-based culture media The tested plant-based culture media successfully supported the culturing of rhizobacteria

Ngày tải lên: 14/01/2020, 14:25

... Trang 6Table.1 Performance of tissue cultured raised Banana varieties on plant height at different growth stages Days After Planting (DAP) Table.2 Performance of tissue culture raised Banana varieties ... After Planting (DAP) Trang 7Table.3 Performance of tissue culture raised Banana varieties on leaf length (cm)at different growth stages Days After Planting (DAP) Table.4 Performance of tissue culture ... After Planting (DAP) Trang 8Table.5 Performance of tissue culture raised Banana varieties on leaf area (m2) at different growth stages Days After Planting (DAP) Table.6 Performance of tissue culture

Ngày tải lên: 20/09/2020, 19:22

... with 5 explants each were considered) After 45 days plantlet length (cm), leaf number/ plantlet, plantlet fresh weight (g), root number / plantlet and root length (cm) were recorded Culture condition ... growing media on plant length (cm), leaf number/ plant, root number/plant and root length(cm) of Hydrangea macrophylla ex vitro length (cm) Leaf number/ plantlet Root number/ plantlet Root ... as explants Experimental treatments Sterilization of explants Different explants (shoot tip and nodes) of macrophylla were washed under a running water for one hour After washing the explants

Ngày tải lên: 23/09/2020, 00:36

... fertilizer in tissue culture banana cv Ney Poovan Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoo n crop Plant crop Ratoon crop Plant crop Ratoon ... girth Suckers production per pit Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoon crop Plant crop Ratoon crop Interaction ... tissue cultre plants, choosing the right plant density, right inter cultural practices, water and fertilizer management are important to gain commercial yield per unit area Modified planting system

Ngày tải lên: 14/10/2020, 18:20

... (2.0) Full plantlet in soil Kawale and (0.1) Full plantlet in soil Kim et al., (2010) (0.3) Full plantlet in soil Komal (2011a) Coconut milk (15%) Full plantlet in soil Komal (2011c) Full plantlet ... Indian medicine system of Ayurveda (AYUSH) Plant tissue culture techniques have been used extensively for propagation of cucurbitaceae by using various explants and methods from last few decades ... potential in efficient clonal propagation Improvement of plant species via biotechnological approach depends largely on plant tissue culture Micropropagation helps to overcome the problems in

Ngày tải lên: 04/11/2020, 22:38

... safe culture: theory and practice” of Reason (1998) introduced the concept of Just Culture as one of five components of Safety Culture along with Informed culture, Reporting Culture, Flexible Culture ... Research ethics 24 CHAPTER III: JUST CULTURE PRACTICE IN THE AVIATION INDUSTRY 25 3.1 History of Just Culture in the world aviation industry 25 3.2 Just Culture Practice in the world aviation 26 ... Safety culture definition 6 1.2 A Just Culture definition 7 1.3 Benefits of Just Culture 8 1.4 Principles of Just Culture 9 1.5 Distinguish acceptable and unacceptable behaviors 11 1.6 Just Culture

Ngày tải lên: 15/01/2021, 00:02

... safe culture: theory and practice” of Reason (1998) introduced the concept of Just Culture as one of five components of Safety Culture along with Informed culture, Reporting Culture, Flexible Culture ... Research ethics 24 CHAPTER III: JUST CULTURE PRACTICE IN THE AVIATION INDUSTRY 25 3.1 History of Just Culture in the world aviation industry 25 3.2 Just Culture Practice in the world aviation 26 ... Safety culture definition 6 1.2 A Just Culture definition 7 1.3 Benefits of Just Culture 8 1.4 Principles of Just Culture 9 1.5 Distinguish acceptable and unacceptable behaviors 11 1.6 Just Culture

Ngày tải lên: 04/04/2021, 19:39

... techniques 9 2.3 The scientific basis of tissue culture 11 2.3.1 Scientific basis of plant cell culture 11 2.3.2 Plant cell culture techniques 12 2.3.3 Culture medium 12 2.4 Research situation ... of the plant can return to the embryonic form and strongly divide (dedifferentiation), then forming a complete body (redifferentiation) 2.3.2 Plant cell culture techniques Plant tissue culture ... methods: sprout separating, cutting branches from selected mother plants in the garden, tissue culture, cutting from tissue cultured mother plants The results are as follows: the traditional methods

Ngày tải lên: 06/07/2023, 22:10

... problems in in vitro culture 20 2.10.5 Stages in propagation culture 21 2.11 Factors affecting the process of plant tissue culture 22 2.11.1 Explants 22 2.11.2 In vitro culture requirment ... individual plant species as well as on the environmental Trang 30composition When tissue cultured plants grow, Parts of plant are often cut and transferred to a new medium (subcultured) to allow plants ... 2.10.1 In vitro method Plant tissue culture is a combination of techniques used to maintain and grow plant cells, tissues or organs under sterile conditions on nutrient-rich culture media with ingredients

Ngày tải lên: 11/07/2023, 14:14

... safe culture: theory and practice” of Reason (1998) introduced the concept of Just Culture as one of five components of Safety Culture along with Informed culture, Reporting Culture, Flexible Culture ... Safety culture deñinition A Just Culture definition Benefits of Just Culture Distinguish acceptable and unacceptable behaviors Just Culture đeeision tree Creating and implementing Just Culture ... safety culture can be engineered It is argued that a safe culture is an Informed Culture and this, in tum, depends upon creating an effective Reporting Culture that is underpinned by a Just Culture

Ngày tải lên: 31/05/2025, 13:28