networks involved in the control of movement

... that the main functional roles of PCA3 occur in the nuclei and microsomal cell fractions Conclusions: Our findings suggest that the ncRNA PCA3 is involved in the control of PCa cell survival, in ... undergo uncontrolled division, and is one of the first steps in carcinogenesis In order to investigate the putative involvement of PCA3 in control-ling this early step of tumorigenesis, we investigated ... cultured in DMEM containing 10% FBS All these cell lines, except PrEC, were cul-tured in the presence of 100 U/mL penicillin and 100 μg/mL streptomycin Cell cultures were maintained at 37°C in a

Ngày tải lên: 05/11/2020, 07:41

... 4) The presence of HIF-1-binding sites in the promoter of MCP-5 gene and the observation that the expression of MCP-5 correlated with the levels of HIF-1α suggest that HIF-1α is involved in transcriptional ... up-reg-ulation of inflammatory mediators also initiates an inflammatory process Infiltration of peripheral inflam-matory cells into the brain is a critical step in the develop-ment and progression of the ... (Table 2), the binding of HIF-1 protein complex to a typical HIF-1-bind-ing consensus DNA sequence [15,16] was examined by EMSA as described in the Materials and methods HIF-1 protein complex in nuclear

Ngày tải lên: 19/06/2014, 22:20

... down-regulation of NgR1 in matDCs promotes their adhesion to myelin, resulting in the selective accumulation of matDCs rather than immDC in the myelin debris-containing lesion This would result in further ... This could indicate a possible role for NgR1/2 outside the CNS, perhaps in the activation of DCs or in homing of DCs to specific tissues This is supported by the findings of the non-myelin associated ... subunits of the NgR1 complex remain to be identified Binding of the NgR1 inhibitory complex by MAIFs leads to activation of intracellular RhoA, thereby resulting in axonal outgrowth inhibition,

Ngày tải lên: 19/06/2014, 22:20

... associated with induction of T-cell tolerance and immune inhibition in the induction of oral tolerance in the murine CIA model We examined the change in the expression of IDO in CD11c+ DCs of Peyer's ... and erythema from the ankle to the tarsal bone, and 4 = edema and erythema from the ankle to the entire leg The sum of the values from three legs, excluding the hind leg into which CII–incomplete ... tolerized mice in collagen-induced arthritis (CIA) We examined whether IDO-expressing dendritic cells (DCs) are involved in the generation of CD4+CD25+ regulatory T cells during the induction of oral

Ngày tải lên: 09/08/2014, 10:22

... in the inhibi-tory effect of Bee venom on the LPS-induced, and in the inhibitory effect of melittin on the SNP-induced NF-κB in THP-1 cells by 10 μM of SP600125 JNK inhibitor suppressed the inhibitory ... activation of MAP kinase is significant in the regulation of inflammation via controlling the activa-tion of NF-κB and IKKs [19-22] In the present study, we therefore investigated whether melittin and ... inhibition of NF-κB is the inhibition of the nuclear translocation of p50 and p65 through the blockage of IκB release To study the result of JNK inhibitor suppressed the inhibitory effect of melittin

Ngày tải lên: 11/08/2014, 08:22

... conditions, the lines expressing the VvMYB60 gene displayed a rate of water loss indistinguishable from the one observed in the wild-type, thus demonstrating complete rescue of the stomatal defects of the ... stage, the onset of ripening and the accu-mulation of sugars are correlated to increasing levels of ABA in the berry [30], suggesting a possible negative effect of the hormone on the expression of ... MYB60 proteins and other closely related proteins from subgroup 1: a distinctive PHEEG sig-nature in the MYB domain, located in the loop connecting the R2 and R3 repeats, and the lack of the first

Ngày tải lên: 11/08/2014, 11:21

... overexpres-sion of AtHAP2 delays flowering via down-regulation of FT [34] In SD-flowering rice plants, the CCT-domain containing proteins Hd1 and Ghd7 regulate flowering by repressing expression of the florigen ... which autono-mously starts independent of the development of the sink, but is accelerated by the sink formation Delaying leaf senescence in order to maintain the photosynthetic activity for as long ... hypothetical protein and hypothetical protein in the RAP-DB We divided the organ/tissue-specific genes into 7 clusters based on the organ/tissue specificity of expression The majority of the

Ngày tải lên: 11/08/2014, 11:21

... [12] Members of the AtNPR family encode proteins sharing two domains involved in mediating protein-protein interactions: the Broad Complex, Tramtrack and Bric a brac/Pox virus and Zinc finger (BTB/POZ) ... Zinc finger (BTB/POZ) domain in the N-terminal and the Ankyrin Repeat Domain (ARD) in the middle of the protein Whereas AtNPR1 to AtNPR4 have been implicated in signalling of defense responses, AtNPR5 ... leading to the induction of these responses remain to be characterized in this species Recently, two genes encoding transcription factors of the WRKY family and potentially involved in grapevine

Ngày tải lên: 12/08/2014, 03:20

... factors) is involved in the control of HIV-1 infection at the maternofetal interface The decidua could thus serve as a mucosal model for identifying correlates of protection against HIV-1 infection ... Invasive placental cells interact directly with decidual cells of the uterine mucosa during the first months of pregnancy, but the role of the decidua in the control of HIV-1 transmission is ... respectively) These results showed that dAPC were the main sources of CCL-3 and CCL-4 in the decidua Decidual soluble factors can inhibit HIV-1 infection To examine the role of the cytokine environment in

Ngày tải lên: 13/08/2014, 01:21

... Diagram of silastic H-shaped prosthesis inserted into the portal vein and the inferior vena cava of the in vivo rabbit model Figure 1 Diagram of silastic H-shaped prosthesis inserted into the portal ... 5The effect of intra-portal injection of (a) ATP and (b) adenosine on changes in hepatic arterial flow (∆ HAF) in vivoFigure 3 The effect of intra-portal injection of (a) ATP and (b) adenosine ... flowmeters in ml min-1 and subsequently recalculated in ml min-1 100 g-1 by relat-ing the readrelat-ings to the wet weight of the liver, determined at the end of each experiment The "buffering capacity"

Ngày tải lên: 13/08/2014, 13:20

... structure of 3'-UTR of cofilin mRNA Figure 4 RNA secondary structure of 3'-UTR of cofilin mRNA The arrow indicates the U-rich signal sequence that could be involved in the localization of the messenger ... for the localization of the mRNA The sequence is a tandem repeat of an ACACCCACACCC motif The mRNA of β-actin has been located in the axon of the neuron and in dendritic spines [12,14] β-actin ... the kinesin family, by analogy with the translocation of the tau messenger by the kinesin KIF3A [11] When the complex reaches the correct destination, the mRNA is translated mRNAs that lack the

Ngày tải lên: 13/08/2014, 23:20

... accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences ... %, with the variance in the VG interaction remaining virtually Trang 10unchanged By combining S2 and S3, the Gene × Array interactions occurring specifically on S2 and S3 are pooled They are, ... part of the variance in our data is caused by varying factors (e.g differences in the days on which the experiments were performed; discussed in more detail below) In addition, the quality of the

Ngày tải lên: 14/08/2014, 14:21

... Distribution of fluorescein–labeled moesin siRNA after in vivo delivery of moesin siRNA by iontophoresis into the cornea……….52 10 Effect of moesin siRNA on the expression of moesin in the corneal ... 5 minutes 2 Wash the slides in distilled water for 2 minutes 3 Stain the sections in hematoxylin for 2-3 minutes (staining time can be modified depending on desired staining density) 4 Wash the ... (staining time can be modified depending on desired staining intensity 8 Rinse in 95% ethanol for 3-5 dips, then dehydrate the sections in 95% ethanol for 2 minutes 9 Follow by rinsing in 100%

Ngày tải lên: 09/09/2015, 18:51

... 1EXPLOITING THE INHERENT COORDINATION OF CENTRAL PATTERN GENERATOR IN THE CONTROL OF HUMANOID ROBOT WALKING Trang 2Exploiting the Inherent Coordination of Central Pattern Generator in the Control ofHumanoid ... The circle in the subplot of body velocity shows the ve-locity changes because of the push; the dashed circle in the subplot of swing period shows the adjustment of swing period during the push ... onboard computing Thirteen of the joints are the most relevantfor walking: six in each leg in the standard configuration for 6 DoFs humanoid robotlegs, and one in the waist for yaw motion of the waist

Ngày tải lên: 11/09/2015, 10:01

... for the cloning of their shRNAs and measurements of OCT4 / Nanog promoter activity in their knocked down cells (See Appendix) The rationale behind the use of the luciferase assay is as such: In ... status of the ES cell upon Lefty2 RNAi knockdown compared to the control As a start, constructs containing either the Oct4 promoter or Nanog promoter that contain OCT4/SOX2 binding sites driving the ... conditioned by the Lefty2 overexpressing cells by Western blotting using anti-V5 antibody No signal was detected in the control The level of Lefty2 proteins in duplicate preparations of medium conditioned

Ngày tải lên: 11/09/2015, 16:02

... represses the IRF1-mediated activation of the HIV-1 ISRE element Implication of IRF8 in the maintenance and NCoA3 in the reactivation of the viral latency may thus provide new insights into the control ... for the low level of transcription observed during post-integration latency (reviewed in [9]): the inaccessibility of the integrated provirus to the transcriptional machinery, the absence in resting ... this study, the role of the HDAC inhibitor NaB on HIV-1 latently infected cells gene expression was explored using microarrays Since chromatin remodeling is involved in the regulation of HIV-1 gene...

Ngày tải lên: 13/08/2014, 09:21

... aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify ... making it less effective Ginger taken with anticoagulants may increase the risk of bleeding Ginkgo taken with anticoagulants or with aspirin or other NSAIDs may increase the risk of bleeding Ginkgo ... metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen radical-forming enzymes, those acting as ATP mimics and inhibitors of...

Ngày tải lên: 25/10/2013, 05:20

... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP binding sites are located in narrow clefts at the interfaces ... to these interfaces is located in the PYR and middle domains The interface contains 10 hydrogen bonds in IPDC The dimer–dimer interface in IPDC is smaller than the corresponding interface in...

Ngày tải lên: 20/02/2014, 11:20

... listed in Table that most, if not all, of the amylose is synthesized in vivo when the rate of amylopectin synthesis becomes minimal At these moments the rates of amylopectin synthesis are only 5% of ... rate of amylopectin synthesis The increase in crystallinity witnessed during these first 12 h is in line with the high rates observed for amylopectin synthesis However between 12 and 58 h the rate ... amylose continues to accumulate at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]) In vitro synthesis of amylose Because in Chlamydomonas...

Ngày tải lên: 22/02/2014, 07:20

... success in expressing a recombinant XDH in this organism Expression of XDH requires the incorporation of the MPT center, placement of the terminal sulfur ligand on the Mo, and the incorporation of ... stoichiometric and independent of the presence of xdhC, and their data suggest that the level of MPT sulfuration is lower in the absence of xdhC than in its presence Assuming that the level of incorporated ... effect of the level of culture aeration on the expression of recombinant protein Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase...

Ngày tải lên: 16/03/2014, 23:20