molecular epidemiology and evolutionary genetics of infectious diseases

... and cell culture and better than ELISA in terms of sensitivity and specificity Another strategy for enhancing the sensitivity and specificity of QCM-based biosensors involves fabrication of molecular ... improvement of specificity and sensitivity of a given device depends largely on the proper selection and combina-tion of capturing elements and transducers With continuing advances in biochemistry and molecular ... Integrated microfluidic system for multiplexed detection of HIV and syphilis (A) Photograph of microfluidic chip (B) Cross-section of microchannels Scale bar, 500 mm (C) The design of channel meanders...

Ngày tải lên: 13/05/2014, 13:59

... and Antigenic characterization of full genome of seasonal and pandemic 2009 influenza viruses circulating on campus 6.3.2.1 HA and NA diversity 6.3.2.2 Diversity of internal genes 6.3.3 Pandemic ... Sensitivity of molecular and viral isolation methods for detection of influenza A virus infection during the period of surveillance and the methods employed 77 Table 5.3 Comparison of sensitivity of ... CHARACTERIZATION, MOLECULAR EPIDEMIOLOGY AND TRANSMISSION OF INFLUENZA VIRUSES AMONG UNIVERSITY STUDENTS AND STAFF IN Trang 2I Trang 3 Besides my supervisor and my co-supervisor, rest of my Thesis...

Ngày tải lên: 09/09/2015, 08:18

... L E Open AccessComparative population genetics and evolutionary history of two commonly misidentified billfishes of management and conservation concern Andrea M Bernard1, Mahmood S Shivji1*, ... 50 μL and contained 1 μL of un-quantified extracted genomic DNA Final concentration of the remaining PCR reactants were 1 x PCR buffer (0.15 mM MgCl2), 0.2 mM of each dNTP, 0.25 μM of each of the ... spearfish (i.e WNA and WSA) and for pooled samples from each species Priors included the implementation of the TVM + I + G and HKY + I + G models of substitution for roundscale spearfish and white marlin...

Ngày tải lên: 27/03/2023, 04:27

... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... Ca atoms of the catalytic triad, are positional homologs of the R148 and K300 residues of P furiosus archaeal primase, both of which are known to play a pivotal role in the activity of this protein ... side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-prim-ase is part of a motif which...

Ngày tải lên: 18/02/2014, 18:20

... two isoforms of the protein, only the molecular mass of 50 kDa would suggest that we had purified the truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant ... specific 5¢-and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no D11350 and S70040) Primers for the short isoform of b-fructofuranosidase ... werepreincubated with 10 lg of purified glycopeptide and 100 lg of allergen extract before incubation of the blot strips Circular dichroism (CD) spectroscopy of natural and recombinant b-fructofuranosidase...

Ngày tải lên: 21/02/2014, 00:20

... 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and ... proteins in LSDs and a notable number of LSD-like diseases of unknown etiology, there is a com- mon interest in the identification of the proteome of the lysosomal compartment and of the soluble ... and cellular function of the putative lysosomal SC Scpep1, we analysed the molecular properties of Scpep1 and generated an Scpep1 gene trap (Scpep1-gt) mouse model. Results Molecular forms of Scpep1 In...

Ngày tải lên: 07/03/2014, 03:20

... King Professor of Internal Medicine, Chair of the Department of Medicine, and Professor of Microbiology, New York University School of Medicine, New York RICHARD D CLOVER, MD, Dean and Professor, ... MD, Director, Division of Infectious Diseases, Eastern Virginia Medical School, Norfolk RANDALL R REVES, MD, MSc, Professor, Division of Infectious Diseases, University of Colorado Health Sciences ... Associate Professor, Department of Medicine, University of Washington School of Medicine, Seattle MEGAN MURRAY, MD, ScD, MPH, Assistant Professor of Epidemiology, Harvard University, School of Public...

Ngày tải lên: 22/03/2014, 16:21

... detected, and the band intensity of SsHCE2 tran-scripts was about half that of SsHCE1 For the wSsLCE gene, the 33 cycles of RT-PCR gave these bands at about 700 bp, 800 bp, 1 kbp, and 1.2 kbp, ... that of black rockfish [15] From both the species, SsHCE1 and SsHCE2 or-thologs (HhHCE1 and HhHCE2 for H hilgendorfi, and SgHCE1 and SgHCE2 for Set guentheri) were cloned (Fig 5) HCE (GaHCE1 and ... showing cancellation of splicing of intron 1 and intron 5, respectively wSsLCE6 cDNA was 184 bp shorter than wSsLCE5 cDNA, due to partial deletion of exon 5 and partial inclusion of intron 5 wSsLCE6...

Ngày tải lên: 23/03/2014, 07:20

... at 25
C , 30
Cand 35
Cin order to identify scalar and dipolar connectivities among heme and amino acid residues Spectral widths of 13 kHz and mixing times of 80 ms for NOESY and 50 ms for TOCSY ... Tyr66(E7) (G) and Leu70(E11) (E), and to NHs of heme-residue contacts Tyr66(E7) and Phe46(CD1) to have propionates (A, B, C) and interresidue con-tacts from Tyr66(E7) to Phe46(CD1) and NHs of Arg48(CD3) ... Trang 1H NMR study of the molecular structure and magnetic properties Weihong Du1, Zhicheng Xia1, Sylvia Dewilde2, Luc Moens2and Gerd N La Mar1 1 Department of Chemistry, University of California,...

Ngày tải lên: 23/03/2014, 17:22

... migration distances from 100 The Biosys-1 program of Swofford and Selander [29] was used: - to calculate the coefficients [20, 21] of genetic identity (I) and genetic distance (D); - to construct the ... Manwell and Baker [19] and Maguire and Fielder [18] reported a similar hemocyanin pattern of various Crustacean species Furthermore, in the case of Sphaeroma, the hemocyanin is both specific and ... subspecies. The results of the genetic analysis confirm our morphological observations and our tests of tentative hybridization and reinforce our hypothesis of the distinction of three subspecies,...

Ngày tải lên: 09/08/2014, 18:21

... regression line slope of time and the logarithm of OD600 SDS-PAGE and western blotting The equivalent of 200μl of cells at an OD600 of 0.7 was collected by centrifugation and lysed using Novagen’s ... The Causes of Molecular Evolution New York: Oxford University Press 1991. 5 Ayala FJ: Molecular clock mirages Bioessays 1999, 21:71-75. 6 Zuckerkandl E: Evolutionary processes and evolutionary ... activities of the mutants (in percentages) are shown in the table relative to WT. Figure 1 Expression of destabilizing mutants and wild-type LacZ (a) SDS-PAGE of soluble and insoluble fractions of cells...

Ngày tải lên: 09/08/2014, 22:23

... Global Distribution of Yellow Feverand Dengue D.J Rogers1, A.J Wilson1, S.I Hay1,2and A.J Graham1 1 TALA Research Group, Tinbergen Building, Department of Zoology, University of Oxford, South Parks ... altitudinal and population exclusions indicating areas at risk according to species of Plasmodium. Trang 8Plate 5.2 A–R First level administrative division boundaries and P falciparum (Pf) and P vivax ... to presence or absence of any of the main three mapping criteria used (Ad, administrative, Alt, altitude and Urb, urban).cThe mean P falciparum ratio as used in Figures 1 and 2A–R (see colour plate...

Ngày tải lên: 10/08/2014, 08:20

... malaria Trang 10Climate Change and Vector-BorneDiseases D.J Rogers1 and S.E Randolph2 1 TALA Research Group, Tinbergen Building, Department of Zoology, University of Oxford, South Parks Road, Oxford ... seaports of the World and (b) climatic similarity den-drograms for the major airports of the World In both figures the inset close-up shows the branches of significance to the dispersal of Ae Albopictus ... Tinbergen Building, Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK Abstract 346 1 The Mathematics and Biology of Changes in Vector-Borne Diseases 346 2 Defining...

Ngày tải lên: 10/08/2014, 08:20

... rice and Arabidopsis thaliana, and are dis-tributed non-randomly throughout the genome [30-32] Clustering of R genes is known to facilitate tandem dupli-cation of paralogous sequences and generation ... mapping, and the potential for correlation withthe locations of known disease resistance loci Disease resistance loci of cereal species are conserved atthe chromosomal and molecular level [40,41], and ... using Transeq software Each pep-tide sequence was scanned against the Pfam database [51,52] for the presence of known domains, the type, sizeand position of NBS domains and the number of LRRrepeats...

Ngày tải lên: 12/08/2014, 03:20

... Nanotechnology is a branch of science and engineering devoted to the design and production of extremely small electronic devices and circuits built from individual atoms and molecules • Nanotechnology ... Mobile wireless devices support: – Wide range of applications spanning voice and data communication – Remote monitoring, and position finding • Examples of current mobile wireless: – Cell phones, ... Introduction of Nanotechnology What is Nanotechnology Intervention of Nanotechnology Ethical Issues in Nanotechnology Topics Covered (2) • Nanotechnology and Wireless Computing Strategies • Effects of...

Ngày tải lên: 15/03/2014, 23:53

... Modes Credential Handoff to Microsoft ASP.NET 2.0 Impersonation demo Securing ASP with ASP.NET 2.0 Wildcard mapping demo Custom HttpHandler demo ASP.NET Trust Levels Medium trust and Access demo ... DefaultHttpHandler Configure custom handler Custom Request Handler for ASP ASP.NET Trust Levels ASP.NET Trust Levels Code access security Range of named trust levels ... Custom HttpHandler Only needed to handoff custom information from ASP.NET to ASP Role information from an IPrincipal Additional information about the user Derive from DefaultHttpHandler Configure...

Ngày tải lên: 08/07/2013, 01:27

... consists of instructions that accomplish specific tasks The degree of complexity of a PLC program depends upon the complexity of the application, the number and type of input and output devices, and ... to a type of computer commonly used in commercial and industrial control applications PLCs differ from office computers in the types of tasks that they perform and the hardware and software they ... SIMATIC software with STEP and numerous engineering tools supports all phases of product deployment, from hardware configuration of the system and parameterization of modules to service of the...

Ngày tải lên: 15/09/2013, 21:26

... number of directory entries and files, without degrading system performance Gone are the days of needing to add volumes to your server and realizing that you have already maxed out the number of ... Services These services provide the standard and direct input and output to and from objects, as well as other operations on objects themselves, such as create, delete, and truncate operations • Management ... variety of tasks, including locking, managing volume operations, and the addiiton and registration of new objects O c t o b e r 0 63 Semantic Agents The Semantic Agent layer contains loadable software...

Ngày tải lên: 09/12/2013, 17:15

... workarounds for the problems, and improve the efficiency of their work Thus, the adoption of this approach by Microsoft is well justified, since collecting vast amounts of error reports on the areas ... registry and, at the same time, enforce these settings for all users who log on in the local system, the following: Start Regedit.exe and expand the HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\PCHealth\ErrorReporting ... Startup and Recovery option group at the Advanced tab of the System Properties window The Startup and Recovery window will open (Fig 4.10) Figure 4.10: The Startup and Recovery window At the top of...

Ngày tải lên: 14/12/2013, 10:15

... conversion of androstenedione, and 0.05 mg is from peripheral conversion of DHEAS The ovary and adrenal gland secrete about equal amounts of androstenedione and DHEA Thus, about two thirds of a woman’s ... 30%, and a female fetus is at risk of virilization HYPERANDROGENISM OF ADRENAL ORIGIN Excess androgens from the adrenal glands may be the result of neoplasia, inborn errors of biosynthesis of adrenal ... reproductive age HYPERANDROGENISM The primary androgen produced by the ovaries is androstenedione, and the primary androgen from adrenal glands is dehydroepiandrostene sulfate (DHEAS) Androgen production...

Ngày tải lên: 10/02/2014, 14:37