mass spectrometry for biopharmaceutical development

... Wehr, and Mingde Zhu 161 10 Mass Spectrometry for Biopharmaceutical Development Alain Balland and Claudia Jochheim 227 11 Analytical Techniques for Biopharmaceutical Development — ELISA Joanne ... and thus require a tailored developmental scheme In other words, what works for the development of one drug does not necessarily work for the development of another one For example, highly specific ... the Editors Contributors vii ix Analytical Techniques for Biopharmaceutical Development Stephen Tuck Introduction to the Development of Biopharmaceuticals Roberto Rodriguez-Diaz Protein Assay...

Ngày tải lên: 29/06/2014, 11:20

... (5S)-[5-2H1]F420H2 is for a : mixture of [5-1H]F420 and [5-2H]F420 The other two (a, c) are for [5-1H]F420 (C) Experiment with NaB2H4-reduced [5-1H]F420 showing that the mass spectrum of F420 formed from ... oxidation product (c, white) are for a : mixture of [5-1H]F420 and [5-2H]F420 (Fig 2Bb, stick spectrum, black) For comparison the relative intensities calculated for a : mixture are given (Fig ... Chemischen Industrie Henning Seedorf thanks the Deutsche Forschungsgemeinschaft for a graduate fellowship We are indebted to Christoph Hagemeier for providing purified F420-dependent Mtd from Methanopyrus...

Ngày tải lên: 20/02/2014, 03:20

... Absorption Spectroscopy For more information on our products and services, visit our Web site at www.agilent.com/chem/icpms Agilent shall not be liable for errors contained herein or for incidental or ... Reaction System (ORS) works well for the removal of carbon-based interferences The ORS employs simple reaction gases (H2 and He), and therefore does not suffer from the formation of new “cluster” species ... Table shows typical oxygen concentrations and sample introduction configurations used for a range of solvents for which routine methods have been established Even highly volatile solvents such as...

Ngày tải lên: 05/03/2014, 21:20

... information has made mass spectrometry and biology much more compatible than in the past The Ôjoined forcesÕ of sophisticated biochemical and mass spectrometry approaches are the real driving forces ... Medical School, personal communication) Protein identification by mass spectrometry Mass spectrometry is the method of choice for the identification of proteins in proteomics projects because of ... may be Ó FEBS 2003 Affinity purification -mass spectrometry (Eur J Biochem 270) 575 Table Merits of mass spectrometry based protein identification strategies used for the analysis of protein complexes...

Ngày tải lên: 31/03/2014, 07:20

... detection such as mass spectrometry (MS) in contrast to the mostly applied ELISA analytical methods applied yet For a sensitive detection, including structural information, tandem mass spectrometry ... ng/mL solution for each labelled standard [2H4] LTB4 was used for the correction of leucotriene response values, whereas [2H4] PGE2 was used for prostaglandins and 8-isoprostane For quantification, ... described in Methods Mass spectrometry A Sciex API 3000 tandem MS system was used for MS-MS detection with an electrospray ion source in the negative ion mode (ESI-) Compound specific mass spectrometer...

Ngày tải lên: 20/06/2014, 00:20

... according to the formula: yB + * sB and were 0.2 μg/L for 2,4-TDA, 0.2 μg/L for 2,6-TDA, 0.3 μg/L for 4,4’-MDA, 1.0 μg/L for 1,5-NDA, 1.0 μg/L 1,6 HDA and 1.0 μg/L for both IPDA isoforms The levels ... respectively; the specific ions m/z (mass/ charge) was 123 (m/z for target ion) and 286 (m/z for qualifier), respectively; for 4,4’ MDA the RT was 18.47 and m/z was 490/ 252; for 2,4- und 2,6-TDA: 10.33 ... standard deviations for the patient samples detected with mass spectrometry (analysed against analytical standards for each individual diamine) The trend curves are shown for the low, 496 ± 103...

Ngày tải lên: 20/06/2014, 00:20

... format peaklists using “Quant” module from MaxQuant’s (v 1.0.13.13, Max-Planck Institute for Mass Spectrometry, Martinsried, Germany [13,14] The MSM files are peaklists with high precursor mass ... was next placed in trypsin solution for digestion of proteins to peptides for subsequent processing and mass spectrometry analysis See Experimental Methods for details Kooren et al Clinical Proteomics ... J.A.K We also thank the Center for Mass Spectrometry and Proteomics at the University of Minnesota for instrumental resources and the Minnesota Supercomputing Institute for computational support Author...

Ngày tải lên: 13/08/2014, 13:20

... variety of 17 separation methods with mass spectrometry (MS), which consists of an ion source for volatizing proteins or peptides, a mass analyser for measuring the mass- to-charge ratio (m/z) of the ... analyser (to generate the MS1) before the mass- selected ions are transmitted to the collision cell for analysis for further fragmentation before analysis by the second mass analyser (to generate the ... friendship, support and hearing ears whenever I whine Siok Ghee, for her expertise in mass spectrometry and Rachel for her help in all the bioinformatics and visualization plots Past and present members...

Ngày tải lên: 10/09/2015, 09:04

... spectra data generated by mass spectrometry (MS) or tandem mass spectrometry (MS/MS) The advance in tandem mass spectrometry (MS/MS) technology has made highthroughput mass spectra generation possible ... special focus on algorithms for peptide sequence identification by mass spectrometry Traditionally, there are two classes of algorithms for peptide identification by mass spectrometry problem aim ... algorithms for peptide and PTM identification using tandem mass spectrometry The main concern for algorithms in peptide identification is achieving fast and accurate peptide identification by mass spectrometry...

Ngày tải lên: 12/09/2015, 08:18

... secondary ion mass spectrometry- On the sample surface, an energy-rich primary ion beam generates secondary ions, which are separated and detected with a mass spectrometer Secondary ion mass spectrometry ... 1.1 The need for high resolution Secondary Ion Mass Spectrometry( SIMS) 1.2 Practical issues and solutions to accurate SIMS depth profiling 1.3 Main focus of this thesis Reference for Chapter 1 ... mixing effect and Symmetric reflection transformation for SQW structure 4.4 Conlusion Reference for Chapter Chapter Results and discussion II- ∆z2roughn for 500eV O2+ beam bombardments 5.1 Introduction...

Ngày tải lên: 16/09/2015, 15:23

... mass analyzers provides a versatile set of mass spectrometry methods with surprising sensitivity and accuracy for protein and other biomolecular analysis The true power of mass spectrometry for ... Complexity in Eluate Submitting to Mass Spectrometry 62 3.3.6 Identification of Ku70 Complex by Mass Spectrometry 64 3.3.7 Validation of Search Result of Mass Spectrometry 67 3.3.8 Poly ... inhibitors high-performance liquid chromatography iodoacetamide immunoblotting immunoprecipitation Luria Bertani matrix-assisted laser desorption/ionization mass spectrometry tandem mass spectrometry...

Ngày tải lên: 04/10/2015, 15:46

... AGRICULTURE FOR SUSTAINABLE DEVELOPMENT 3) Both short and long-term strategies a) Investment and accumulation of capital b) Reform of pricing schemes development of open markets and other reforms c) ... systems f) Temperature migration: STRATEGIES TO BE ADAPTED ON AGRICULTURE FOR SUSTAINABLE DEVELOPMENT 2) Long term adaptation a) Development of new technologies and modernization b) Changing crop mix ... temperatures through 20oC, 25oC are very important for defining the crop calendar and crop rotation , especially for defining the growing period for Agriculture in the North and in the Mountain...

Ngày tải lên: 23/09/2012, 15:10

... maturity of the forward contract, the forward price is linked to the current price by the interest cost of holding the asset until the forward contract matures In the case of forward foreign exchange ... foreign and domestic interest rates The forward foreign exchange rate is related to the spot foreign exchange rate by the ratio of (1 +) the home country interest rate relative to (1 +) the foreign ... information about firms; (b) negotiating a contract that will limit the firm’s opportunities for taking advantage of the saver; (c) monitoring the firm’s performance; and, if necessary, (d) enforcing...

Ngày tải lên: 24/10/2012, 09:40

... read the enclosed lists of drugs and diseases before they replied In addition, mothers of cases were asked to give a signature for the enclosed informed consent which authorised us to record their ... 480 486 Crude POR with 95% CI for low birthweight (outcome = low birth weight, exposure = infusion): 2.4 (1.7 – 3.5) Adjusted POR for low birthweight (adjusted for birth order, maternal age and ... (1.6 – 3.4) Crude POR with 95% CI for preterm birth (outcome = preterm birth, exposure = infusion): 2.0 (1.4 – 2.7) Adjusted POR for preterm birth (adjusted for birth order, maternal age and...

Ngày tải lên: 02/11/2012, 11:08

... was used to collect data for all the three types of services: a fastfood restaurant for pure service, an airline for mixed service, and a longdistance telephone company for quasi-manufacturing ... management development To the authors’ knowledge no one has yet attempted to assess the implications of the relationship between service quality and its five dimensions for management development ... customers SERVQUAL, an instrument for measuring service quality was developed on the basis of the above five dimensions SERVQUAL has been gaining acceptance as a platform for work in service quality...

Ngày tải lên: 28/01/2013, 10:57

... Commercial Bank for Industry and Trade • Vietcombank: Vietnam Joint Stock Commercial Bank for foreign trade of Vietnam Agribank: VietnamBank for Agriculture and Rural Development • BIDV: Bank for Investment ... bring enormous significance for both theoretical and practical aspects for the diversification of the bank’s activities For this reason, the issue “Some solutions for the development of consumer ... strength, the weakness as well as the reason for its shortcomings Then, based on the limitations, this report puts forward some suggestions for the development of consumer lending Apart from the...

Ngày tải lên: 25/07/2013, 11:20

... cooperation and financing for development emphasized in: UN Committee of Experts on International Cooperation in Tax Matters (subsidiary body of ECOSOC) Financing for Development Office (FfDO) ... 2008 Doha Declaration on Financing for Development; Outcome of 2009 Financial Crisis Conference; Outcome of 2010 MDG Summit Rio+20 Conference on Sustainable Development Committee of Experts Mandate ... and Developing Countries and the Manual for the Negotiation of Bilateral Tax Treaties between Developed and Developing Countries • Provide a framework for dialogue with a view to enhancing and...

Ngày tải lên: 05/09/2013, 14:19

... Anglin for contacting me to write this book, Debra Kelly for guiding me through the various aspects of this project, Tom Welsh for helping me with the development of my chapters, Paul Connolly for ... Platform Java is a platform for executing programs In contrast to platforms that consist of physical processors (such as an Intel processor) and operating systems (such as Linux), the Java platform ... created his own Java/C++ software for working with smart cards Jeff has written about Java in numerous articles for JavaWorld (www.javaworld.com), informIT (www.informit.com), and java.net (http://java.net),...

Ngày tải lên: 20/11/2013, 21:05

... shaping development • Gentrification? [Helms] MIT Center for Real Estate FAR The spatial Pattern of Economic Redevelopment Re- development 1900 Land Rent At each period 1850 1950 2000 Redevelopment ... MIT Center for Real Estate How does land use “evolve”? • City Development evolves from the center outward – on vacant land at the edge • At each time period, there is a “shadow” value for interior ... FAR: F MIT Center for Real Estate Boston Back Bay Condominium Example • From 1984 regression: R = 222 – 1.48F, for new 2-bed, 2-bath with parking on Beacon hill (178-1.48F for end of Commonwealth...

Ngày tải lên: 09/12/2013, 20:15