linking phylogeny with protein structure and function

... leaf photosynthesis and respiration acclimation, but leaf structure (microstructure and ultrastructure) and biochemical processes were seldom focused on [1,11,14] Because leaf structure is one of ... simulated climatic warming on SOD and CAT activities, contents of MDA, proline, carbohydrates and chlorophyll of Arabidopsis thaliana leaves, and leaf microstructure and ultrastructure, apart from fitness ... leaf structure to warming is fundamental to projecting the impact of global change on plant growth In addition, leaf biochemical and physiological changes are related to leaf structure and function...

Ngày tải lên: 11/08/2014, 11:21

... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without ... fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged gAd-GLP-1-A fusion protein; ... gAd-GLP-1-A fusion protein (Fig 4B) The fusion protein without His-tag was gAd-GLP-1-A fusion protein (Fig 1B) Figure Enterokinase cleavage of the N-terminal His-tagged gAd-GLP-1-A fusion protein (A)...

Ngày tải lên: 25/10/2012, 11:10

... (EMSA) and RdRp assays, and examined the influence of different cis-acting elements on binding of NS5B protein and RNA synthesis in vitro Materials and methods Expression and purification of NS5B proteins ... minus-strand 3¢ UTR), –RNAr2 (a random sequence corresponding to positions 2033–2500 of the minus-strand genome) and minus-strand 5¢ UTR Similarly, we found that the –RNA0 competed most effectively with ... Interestingly, when the plusstrand 3¢ UTR and the minus-strand 3¢ UTR were used at the same time, the NS5B proteins bound to the minusstrand 3¢ UTR more strongly than to the plus-strand 3¢ UTR Although...

Ngày tải lên: 19/02/2014, 16:20

... CP1/CP2 and TP1/TP2 with the remainder of the BLG structure It is evident that all these fragments include a generous portion of the b-barrel structure (strands B, C and part of strand D), along with ... fragments CP1/2 and TP1/2 within the primary structure of BLG Residues susceptible to chymotrypsin and trypsin hydrolysis are labeled with ÔcÕ and ÔtÕ superscripts, respectively Cysteines 66 and 160 ... fragments CP and 2, as well as fragments TP and 2, were further purified by RP-HPLC and SE-HPLC in the same conditions and with the same equipment used for analysis of the whole proteolyzate, and these...

Ngày tải lên: 21/02/2014, 15:20

... (Meta) and amastigotes (Ama), probed with antiserum against TcDHH1 protein (1 : 100 dilution) The extracts were standardized and all lanes were loaded with 10 lg of protein (B) SDS ⁄ PAGE stained with ... various proteins in addition to TcDHH1, including heat shock proteins, mRNAbinding proteins, initiation and elongation translation factors, ribosomal proteins and metabolic proteins Abundant proteins, ... that proteins directly or indirectly associated with TcDHH1 include those of diverse function, e.g translation-related factors, cytoskeleton proteins, heat shock proteins and metabolic proteins...

Ngày tải lên: 15/03/2014, 23:20

... level of protein about mg g−1 FW was then maintained Protein contents Enzyme activities and isoenzyme analysis The total soluble protein content was examined in leaf and root samples Roots and L4 ... of de novo protein synthesis or by the activation of enzymes already present in plant cells (Van Assche and Clijsters, 1990a) Changes in SOD and POD activities, and in the (iso)SOD and (iso)POD ... and Frankland, 1983) and oat (Bjerre and Scierup, 1985) Jalil et al (1994) reported that 0.5 µM Cd in a nutrient solution significantly decreases the Mn and Zn concentrations in the roots and the...

Ngày tải lên: 15/03/2014, 23:22

... development and reduce oocyte size, cause a decrease in total ovary protein incorporation, and suppress ovary total protein synthesis [11,17,18] Biological assays using these criteria are nonspecific and ... injection (Figs 4A and 5A) In conclusion, the use of recombinant protein or RNAi alone may not be sufficient to confirm the function of a neuropeptide The combined use of recombinant protein and RNAi described ... 0.5 m NaCl, and 25 mm imidazole, pH 7.9) The fusion protein was eluted with elution buffer (6 mm Tris ⁄ HCl, 0.5 m NaCl, m urea, and 300 mm imidazole, pH 7.9) The denatured recombinant protein was...

Ngày tải lên: 16/03/2014, 05:20

... d), for h, and then washed and probed with biotin-curdlan (cur), as described in the Materials and methods (1fi3)-b-D-glucan-binding proteins and was therefore termed GLUBPp_SUBDO The protein comprised ... stained with Coomassie Brilliant Blue (Fig 1A) and no change in the banding pattern and their intensities occurred Fig Phosphorylation of a 32 kDa protein after incubation of sponge tissue with ... incubated for h with or without 10 lgÆmL)1 curdlan Protein extracts were then prepared and size-separated by PAGE (8% gel) (A) The gel was stained with Coomassie Brilliant Blue (B) Proteins were...

Ngày tải lên: 16/03/2014, 16:20

... and Protein A–Sepharose beads were removed by centrifugation Supernatants were analysed by SDS/PAGE and gels were stained with Coomassie Brilliant blue, fluorographed with Amplify (Amersham) and ... sGas1E161Q-H and sGas1E262QH mutant proteins with reduced laminarioligosaccharides The recombinant purified proteins were incubated with mM reduced laminarioligosaccharide of size G13, and HPAEC ... biochemical and structural characterization of the protein as a representative of the GH72 family Spectroscopic analyses of the purified proteins were performed, and the behaviour of the purified protein...

Ngày tải lên: 16/03/2014, 18:20

... results using CRP and PCT to determine pulmonary TB and bacterial CAP In conclusion, serum CRP and PCT concentrations differed significantly in patients with pulmonary TB and those with bacterial ... patients with pulmonary TB were younger than those with bacterial CAP, and few had far-advanced disease; this was reflected in the difference in the PORT score between patients with pulmonary TB and ... serology and basic laboratory methods J Infect 1987;14:21-30 11 Hedlund J, Hansson LO Procalcitonin and C-reactive protein levels in community-acquired pneumonia: correlation with etiology and prognosis...

Ngày tải lên: 22/03/2014, 18:20

... urea, GdnHCl and high pressure on the structure and stability of whole particles (bacteriophage MS2 and VLPs) and assembly-defective coat protein species [coat protein dimers (dlFG) and single-chain ... coat protein and RNA has been investigated [5,9,23,24], and amino acid–nucleotide interactions contributing to its stability have been characterized [13,25], but the role of protein protein and protein RNA ... understand the importance of capsid protein protein contacts for the stability of coat protein, we determined the stability of dlFG and compared it with the stability of the genetically fused coat protein...

Ngày tải lên: 30/03/2014, 11:20

... gel top first with water and then with a little stacking gel mixture, and fill the gap remaining in the chamber with the stackmg gel mixture Insert the comb and allow the gel to stand until set ... for two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively Plastic and glassware used in the assay should be absolutely clean and detergent-free ... should be carried out, with shaking, for a minimum of h and preferably overnight When the stain is replaced with destain, stronger bands will be immediately apparent and weaker bands will appear as...

Ngày tải lên: 10/04/2014, 11:03

... compatible with the procedures generally used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex ... mstrument, and can open up new, analytically useful, fragmentation routes Ion-trap and FTMS mstruments are suitable for MS/MS experiments without being combined with other mstrumentation and can ... K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York 16 Hogeland, K E , Jr and Demzer, M L (1994)...

Ngày tải lên: 11/04/2014, 10:11

... requirement of the A12L protein and the AG/A site cleavage for viral replication To begin with, A12L protein expression was confirmed by immunoblot analysis with A12L specific bands obtained only ... of the A12L protein and its essentiality in virus replication Essentiality of A12L protein and AG/A cleavage in VV replication The sequence alignment of the A12L open reading frame with other ... the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV membranes G7L, a phosphoprotein in...

Ngày tải lên: 18/06/2014, 18:20

... anti-inflammatory and may be used for the treatment of RA It inhibits neutrophil elastase and proteinase with high efficiency, as well as cathepsin G, thrombin, trypsin and chymotrypsin with lower efficiency ... TG, Vandenabeele P, Buurman WA: Functional protection by acute phase proteins alpha(1)-acid glycoprotein and alpha(1)-antitrypsin against ischemia/reperfusion injury by preventing apoptosis and ... inhibition of proteinases to prevent tissue injury and joint destruction Human AAT is well known as a serine proteinase inhibitor (serpin) It inhibits proteinase 3, neutrophil elastase, and cathepsin...

Ngày tải lên: 18/06/2014, 19:20

... with PstI and partially digested with NcoI Fragments of both plasmids approximately 0.33 kbp, 0.69 kbp, and 4.4 kbp in size (respectively referred to as K1, K2, and K3 for pKNco and S1, S2, and ... periphery and binds to CP [43], which is reminiscent of at least some begomovirus MPs that have been shown to associate with plasma membranes and cell walls [44,45] and to co-operate with NSP ... enzymes, and maize genotypes The cloning vectors pBluescriptSK+ (pSK+; Stratagene, La Jolla, CA) and pUC19 (Stratagene), and the RecAEscherichia coli strains DH5α and JM109 were used in all standard...

Ngày tải lên: 20/06/2014, 01:20

... measure the progression of atherosclerosis Lipoprotein and CRP levels were measured at baseline and follow-up From the Cleveland Clinic Foundation, Cleveland (S.E.N., E.M.T., P.S., T.C.); Pfizer, ... levels of atherogenic lipoproteins, CRP, and HDL cholesterol and the rate of progres- Table Baseline and Follow-up Values for Intravascular Ultrasonographic End Points and Change in Values from ... C-reactive protein and peptides derived from C-reactive protein J Clin Invest 1997;100:522-9 25 Torzewski M, Rist C, Mortensen RF, et al C-reactive protein in the arterial intima: role of C-reactive protein...

Ngày tải lên: 27/06/2014, 00:20