interaction of fast electrons with matter

... visuali-zation One mole of genistein is bound per mole of HSA with a binding constant of 1.5 ± 0.2· 105m)1 Binding of genistein to HSA precludes the attachment of daidzein The ability of HSA to bind genistein ... Fluorescence quenching studies with genistein Interaction of genistein with HSA has been monitored following the quenching of relative fluorescence inten-sity of HSA Quenching of fluorescence by genistein ... energetics of interactions has been followed by varying binding con-stant with temperature The nature of the interaction was identified by temperature and ionic strength dependence of binding

Ngày tải lên: 23/03/2014, 11:20

... remarkable feature of selenium consists of its ability to oxidize thiols under reducing conditions Thus, one mode of action recently suggested is the oxidation of thiol groups of metal-lothionein, ... present in one of the major classes of transcription factors and other so-called zinc finger proteins Within this study we investi-gated the effect of selenium compounds on the activity of the formamidopyrimidine-DNA ... [34] Determination of the metal content of MT by complete zinc release after oxidation with 10 mM H2O2 and ICP-MS revealed 6.4 zinc atoms per molecule of MT and negligible amounts of cadmium and

Ngày tải lên: 30/03/2014, 15:20

... Abstract The interaction of nanoparticles with biomolecules and microorganisms is an expanding field of research Within this field, an area that has been largely unexplored is the interaction of metal ... the interaction of silver nanoparticles with viruses Herein, we present the first findings of our investigation, the discovery that silver nanoparticles undergo size-dependent interaction with ... observed read shift is a con-sequence of both nanoparticles of larger size and the presence of decahedral nanoparticles with pentagonal cross sections Interaction with HIV-1 High angle annular dark

Ngày tải lên: 11/08/2014, 00:22

... of sensitivity of the plant to the pathogen, the transcriptional profiles should be very similar in the two interactions The aim of this study was to ask whether the transcriptional profile of ... demonstrates that the transcriptional profile of the compatible interaction is not identical to that of the wellstudied incompatible interaction with Col-0 Induction of the monooxygenase gene MO1, and ... brassicicola-Arabidopsis interaction differs from that of an incompatible interaction Differential cDNA screening and a candidate gene approach led to the identification of specific markers for the two types of interaction

Ngày tải lên: 12/08/2014, 03:20

... inhibition of leukaemia virus membrane fusion by interaction of peptide antagonists with a conserved region of the coiled coil of envelope Address: 1 The Biomedical Research Centre, College of Medicine, ... trimer-of-hairpins Moreover, many of the conserved amino acids of the LHR are located on the face of the LHR that interacts with the grooves on the coiled coil By exam-ining the location of substituted ... precursor (C) Detail of the predicted interaction of the HTLV-1 LHR-mimetic peptide (ribbon structure) with the surface of the coiled coil (space filling form) based on the structure of Kobe et al [16];

Ngày tải lên: 13/08/2014, 05:21

... Titration of micelles with fluorophore 614.3.1.2 Disaggregation of FITC-LPS with a detergent 654.3.2 Calculation of titrating a solution of aggregates with a fluorescent probe 664.3.3 Determination of ... treatment with Triton X-100 76 Table 5.2 Comparison of TMR, V4-TMR, V4-TMR: LPS on coverslip 90Table 5.3 Comparison of interaction of V4-TMR peptide with LPS, lipid A Table 7.1 Comparison of height ... concentrations of LPS 89Figure 5.5 Comparison of ACFs of V4-TMR and complexes of V4-TMR with LPS, lipid A and PC 91 Figure 5.6 Comparison of V4-TMR binding to different SUVs 93Figure 5.7 Binding of V4-TMR

Ngày tải lên: 14/09/2015, 21:54

... chloroform with a concentration of 0.2 mM Due to the low solubility of V4 in chloroform, V4 was first dissolved in the minimal volume of methanol to prepare a clear solution Additional chloroform ... then added to prepare a solution with V4 concentration of 0.2mM Required volume of POPG or POPC was mixed with V4 to form lipid/V4 mixture with V4 percentage of 0%, 5%, 10%, 20%, 33%, 50% and ... chloroform and DPPG was dissolved in the mixture of chloroform and methanol (v/v=3:1) with a final lipid concentration of 0.2 mM All the studied antimicrobial peptides were dissolved in water with

Ngày tải lên: 14/09/2015, 23:32

... at least hours for FCS experiments. Interaction of V4-TMR with SUVs. The procedure is similar to that of interaction of V4- TMR with LPS. The SUV solutions with different lipid compositions were ... ratio of 1:40 with an increase in amplitude of the ACF and a slight increase in diffusion time. At a peptide/lipid ratio of 1:20, the shape of the ACF changed markedly with the emergence of a ... ability of lipids to entrap fluorophores inside. 6.3.7 Real time interaction of V4 with rhodamine 6G entrapped POPG LUVs LUVs composed of POPG were used to investigate the real time interaction of

Ngày tải lên: 14/09/2015, 23:32

... maintenance of cell lines 40 Infection of T cells with live bacteria strains 40 Costimulation of bacteria-infected T cells with TCR Costimulation of T cells with bacteria culture Costimulation of T ... 1INTERACTION OF BURKHOLDERIA PSEUDOMALLEI WITH CELLS OF THE IMMUNE SYSTEM LEE MEI LING, CHERYL A THESIS SUBMITTED FOR THE PARTIAL FULFILLMENT FOR THE DEREE OF MASTER OF SCIENCE DEPARTMENT OF ... cells interacting with live B pseudomallei in the absence of direct Absence of costimulatory effect of heat-killed B pseudomallei on IL-2 production by Jurkat T cells 48 Comparison of IL-2 production

Ngày tải lên: 08/11/2015, 16:31

... surface properties of the particles The objective of this work is to investigate the influence of the physico-chemical properties of polymeric nanoparticles on their interactions with cells Emphasis ... monolayer can be correlated to their interaction with biomembranes [16] The influence of the physicochemical properties of Trang 16nanoparticles on their interaction with cells can also be investigated ... Trang 1INTERACTION OF POLYMERIC ANOPARTICLES WITH A MODEL CELL MEMBRANE – A LANGMUIR FILM BALANCE TECHNIQUE SEOW PEI HSING (B.Eng (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING

Ngày tải lên: 08/11/2015, 16:31

... semiquantitative analysis cells of weak intensity × 1) + (percentage of cells of mod-erate intensity × 2) + percentage of cells of strong inten-sity × 3), PI shows percentage of cells of all positive cell ... Compared with control cells, hnRNP K bundling or increased density of microtubules radiating from the perinuclear region with or without TGF-β1 treatment But, knockdown of MAP 1B-LC1 with siRNA ... characterized as a subunit of MAP 1B was found to bind to microtu-bules in vivo and in vitro and induce rapid polymerization of tubulin [17, 18] The interaction of MAP 1B/LC1 with hnRNP K may provide

Ngày tải lên: 17/06/2020, 18:05

... novel interaction partner of fascin-1 These data have important implications for consideration of the role of fascin-1 in carcinoma metastasis Results RhoA supports the interaction of fascin-1 with ... activity does not modulate the interaction of fascin-1 with conventional protein kinase C (cPKC) (A) Measurement of the interaction of green fluorescent protein (GFP)-fascin-1 with PKCa- monomeric red ... kinase activity promotes the interaction of fascin-1 with actin (A) Percentage FRET efficiency of the interaction of monomeric red fluorescent protein (mRFP)-fascin-1S39A with green fluorescent protein

Ngày tải lên: 02/11/2022, 08:48

... absence of inhibitory effects was due to a lack of interaction with AgNP or whether any impact was masked by de-novo synthesis of the enzyme Indeed, studying the interaction of AgNP or AgNO3 with ... However, considering that algae within periphyton are embedded in a matrix of biomolecules forming a biofilm [59], other factors might influence direct interaction of AgNP with the enzyme, e.g periphyton ... protein-particle interactions In order to shed light on the detailed mechanisms of interaction between AgNP and cells of algae and fish, we explored different aspects of AgNP-cell interactions,

Ngày tải lên: 04/12/2022, 14:56

... growth) of each drug.The plates were then processed for the sulforhodamine-B assay, as described [32] Evaluation of synergistic/antagonistic interaction with gemcitabine The pharmacological interaction ... normalized with respect to the untreated cells Analysis of modulation of Glut1 by flow cytometry To quantitatively detect the expression of membrane-bound Glut1, cells were fixed with 80% ethanol, ... spheroids (Fig 2d–e) The combination of perifosine with gemcitabine additionally reduced the size of the LPC028 and PANC-1 spheroids with respect to the spheroids treated with the single drugs In contrast,

Ngày tải lên: 04/12/2022, 16:01

... sequence similarity with MPs of other genera, but showed 32% identity with the MP of South-ern bean mosaic virus within the Sobemovirus genus With a view to understanding the mechanism of cell-to-cell ... similarity with MPs of viruses from other gen-era Within sobemoviruses, the sequence of the SeMV MP was closest to that of the SBMV-Ark MP (32% sequence identity), and the identity with MPs of other ... Fig 4 Interaction of rMP with NV (A) ELISA of rMP and NV inter-action ELISA plates coated with NV (5 lg) (P1) were blocked with 10% skimmed milk in 1% NaCl ⁄ Pi (block) followed by addition of 5

Ngày tải lên: 15/03/2014, 00:20

... were washed twice with buffer C and twice with buffer C with-out NP40 The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubating for 10 min with gentle stirring ... nuclear-enriched fraction of proteins The effi-ciency of labelling of the single oligomers being not homogenous, the ratios of the different binding signals within each lane instead of their absolute ... 4formation of the CRC agrees with the absence of cellgrowth inhibition by GT-G4 By affinity chromatography, using a GT biotinylated 51-mer, the proteins interacting with GT were isolated from the pool of

Ngày tải lên: 16/03/2014, 13:20

... with the 3¢ of the )148 to )124 region of c-jun. However, this interaction was not found to be absolutely essential f or its interaction with RLjunRP c omplexed with Jun-25. The interaction of rRLjunRP ... arance of additional factor i nteracting with )148 to )124 region of c-jun after partial hepatectomy. (A)Timeofappearanceofcomplex C2 after p artial hepatectomy. EMSA were carried out using 1 ng of ... e correlated w ith the appearance of the factor, rRLjunRP, involved in the f ormation of complex C2, observed with rRNE-d, and if the time of appearance of this factor coincides with the increase in c-jun

Ngày tải lên: 16/03/2014, 18:20

... coimmunoprecipitated with the ETAR-1D4, regardless of the absence or presence of ET-1 (lanes 2 and 3), but not without the expression of the receptor (lane 1) This observation is consistent with those of the ... manner To examine the interaction of ETBR with caveolin-1, we took advantage of an insect cell expression system, in which ETAR and ETBR are expressed well, with KDvalues for ET-1 of 55 ± 8 and 83 ... Furthermore, the amount of ETBR localized in caveolae increased significantly with the expression of caveolin-1 and decreased with the addition of ET-1 The disruption of caveolae by filipin reduced

Ngày tải lên: 17/03/2014, 03:20

... factor-a production (Fig. 10). The comparison of the results of the interaction of LPS with HDL to those published for the interaction of the former with another serum protein, albumin, indicates ... sugar units, lipid A two of each) which may be connected with different conformations of the molecules. Differential scanning calorimetry measurements of the interaction of LPS with HDL (Fig. 3) shows ... three types of experiments with LPS Re and HDL have been carried out. Preincubation of the cells withLPSRe(30minat37°C) and following addition of HDL, preincubation of the cells with HDL and

Ngày tải lên: 17/03/2014, 10:20

... indicating that the interactions of these differ-ent proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP Association of 42Sp50 with mRNA has ... that are formed with the corresponding signal sequences in the untranslated regions of the mRNAs and with a number of different so-called localization proteins In the context of the present study, ... 6family of RNA binding proteins [31–33] Together withthe findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs

Ngày tải lên: 23/03/2014, 03:20