infrared imaging for tissue characterization and function

... resonance imaging (MRI), and ultrasound, provide structural/functional imaging information But their performance is limited more and less by their own shortcomings The diffuse optical imaging modalities ... melanin and cytochrome will be ignored 2.3 Optical properties of breast tissues The human breast tissues are majorly constituted by glandular tissue and fatty tissue The concentrations of water and ... optical imaging is a very safe and very comfort imaging modality for breast imaging Using low dose of NIR light for illumination, ionization hazard will not happen at all Patients could repeat imaging...

Ngày tải lên: 12/09/2015, 11:35

... xTRHR2 and xTRHR3) and their functional expression in Xenopus oocytes and mammalian cells We have also determined the tissue distributions of xTRHR mRNA species by RT-PCR This study therefore represents ... Tyr287 and Arg311), and the two Cys residues (105 and 186) that form a disulfide bond between EL1 and EL2 to maintain the receptor in a high affinity conformational state Several Ser and Thr residues ... glycosylation site in EL2 (Asn167 for xTRHR1 and Asn172 for xTRHR2) and the two homologous Cys residues (335 and 337 for xTRHR1, 339 and 341 for xTRHR2) in the C-terminal tail were also found The...

Ngày tải lên: 21/02/2014, 03:20

... exit the ER and reach the cell and/ or virion surfaces to mediate attachment and membrane fusion This phenotype qualified for forced protein evolution studies, with the aim of identifying and investigating ... with 2500 pg CA-p24 and virus spread was measured for 14 days B gp120 and CA-p24 contents in virus were measured by ELISA The gp120 amounts were standardized for CA-p24 input and the gp120 contents ... infected with VVT7 and transfected with plasmids encoding mutant and revertant or wt gp120 Cells were pulse-labeled for and chased for the indicated times Cells were lysed and gp120 was immunoprecipitated...

Ngày tải lên: 13/08/2014, 06:20

... Xiaohang Yang and Bill Chia for accepting me as a graduate student in their lab and for their continual support throughout I am especially grateful to Sami for mentoring me and for giving me ... Tong-wey, and Xavier for all their suggestions for my work and for allowing me to pick their brains, and thankfully still remaining sane at the end of the day I am especially grateful to Kavita for ... 2003; Chia and Yang, 2002) Analyses of both loss and gain of function approaches suggest that Pins is required for maintenance of apical Insc later in interphase and in mitosis and for spindle...

Ngày tải lên: 12/09/2015, 09:42

... required for lateral domain or lipid rafts formation and for regulation of integral membrane protein function, and also for transcriptional regulation (Bagnat, 2000; Pike, 2003; Simons and Ikonen, ... biosynthesis and total ergosterol between wild type and ncr1Δ cells Therefore, this suggests that there may be a synergistic effect between Ncr1p and other ergosterol metabolism and transport ... with them and the students and employees from their labs Deeply from my heart, I would like to thank my wife for her love and support, -2- even at the most difficult time, I was happy and peaceful...

Ngày tải lên: 12/09/2015, 09:42

... bone marrow (37-fold increase for IL-1 treatment and 8-fold increase for TNF-α treatment relative to vehicle), dermal (14fold and 4-fold increase) and synovial (31-fold and 7-fold) fibroblasts (Figure ... probe and 25–50 ng cDNA Target genes probes were labelled with FAM and 18S probes were labelled with VIC Reactions occurred as follows: 50°C for minutes, 95°C for 10 minutes, 44 cycles of 95°C for ... von Willebrand factor staining) Page of 10 (page number not for citation purposes) Figure Autocrine activation of cortisol and the regulation of fibroblast function of fibroblast function Bone...

Ngày tải lên: 09/08/2014, 08:22

... MECHANICAL AND FUNCTIONAL PERFORMANCE OF EXTRUSION-BASED ADDITIVE MANUFACTURED SCAFFOLDS FOR BONE TISSUE ENGINEERING MUHAMMAD TARIK ARAFAT (B.Sc in Mechanical Engineering, BUET) A THESIS SUBMITTED FOR ... during the screw extrusion system development and maintenance, and Andrew for his valuable suggestions I would also like to thank Liu Yuan and Dr Monica for helping me a lot during the initial phase ... (Strut), and non-bony tissue as controls (Tissue) Additionally, push-out tests were performed on host calvarial bone as controls 134 Micro-compression tests and push out tests were performed after...

Ngày tải lên: 10/09/2015, 15:51

... fluorophosphonates for monitoring serine hydrolases [73], and biotinylated a-bromobenzylphosphonates for detecting protein tyrosine phosphatases [74] ATP, ADP and AMP are important sensors for the energy ... Fund for Scientific Research ) Flanders (Belgium) The work in this paper was supported by research grants from the Fund for Scientific Research ) Flanders (Belgium) (project number G.0280.07) and ... Morphological and biochemical characterization of a human liver in a uPA-SCID mouse chimera Hepatology 41, 847–856 Vande Walle L, Van Damme P, Lamkanfi M, Saelens X, Vandekerckhove J, Gevaert K & Vandenabeele...

Ngày tải lên: 18/02/2014, 16:20

... 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... containing the residues predicted to be responsible for polymerase and primase catalysis, and reported on the functional characterization of the main functions of this protein FEBS Journal 275 (2008) ... were able to account for the promiscuous nature of the synthesis functions performed by the prim–pol domain and to discriminate between the functions of in vitro primase and polymerase Another...

Ngày tải lên: 18/02/2014, 18:20

... curves and data were normalized to the copy number of 18S RNA Expression of ACMSD isoforms in E coli pGEM-ACMSD I and pGEM-ACMSD II were digested with XhoI and Bpu1102I and cloned into pET-15b and ... desired modification and to ensure the absence of random mutations The mutagenized plasmids were transformed into P pastoris GS115 competent cells and expression was performed as for the wild-type ... swiss-pdbviewer and visual molecular dynamics [49] Acknowledgements We thank G Magni and S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and Chemical Biology,...

Ngày tải lên: 19/02/2014, 02:20

... between pPYY and endogenous cNPY Nevertheless, the radioligand could be used for competition experiments with a panel of ligands (Table and Fig 8) Porcine PYY competed with the radioligand for binding ... low affinity, as for zebrafish Y7 [25] These differences in ligand affinity between Y7 and Y2 may prove very useful for studies of ligand–receptor interactions and 3D modeling, and we have previously ... the tissue distribution and phylogeny of the chicken Y6 and Y7 receptors and performed the initial pharmacological characterization of the latter It is clear, from these studies, that the Y6 and...

Ngày tải lên: 19/02/2014, 07:20

... Deutsche Forschungsgemeinschaft (NI 592/2-1) We thank Heinrich ganglia neurons and bovine chromaffin cells and assumed to Betz for his support and for critical reading of the manuscript be selective for ... still be present in the snails and could supply novel ligands for mammalian nAChRs (for evolution, diversity and biosynthesis of a-conotoxins see [30,31]) ImI and ImII The first a-conotoxin showing ... situ hybridization and immunohistochemistry data show overlapping distributions for a variety of subunits, and electrophysiological and other functional studies in native tissues have revealed...

Ngày tải lên: 19/02/2014, 12:20

... Asian and Mediterranean countries [15] This paper describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular characterization ... (Amersham) and prehybridized for h at 42 °C in · Denhardt’s solution, · NaCl/Cit, 50 mM sodium phosphate (pH 6.5), 100 lgÆmL)1 salmon sperm DNA, and 50% formamide Membranes were hybridized for 16–20 ... columns, and the insert was sequenced as above Analyses of nucleotide and amino acid sequences were performed using the BLAST algorithm from the database National Center for Biotechnology Information...

Ngày tải lên: 21/02/2014, 00:20

... °C for E190A and 70 °C for E199A and E204A Decreased light scattering after maximum precipitation had been reached resulted from the precipitate sinking to the bottom of the cuvette and therefore ... C-terminal extension remains unstructured and solvent exposed, and therefore able to perform its solubilizing role in the sHsp and in the complexes formed with target proteins during chaperone ... extension of Hsp25 (E190, E199 and E204) are essential for the correct structure and function of this sHsp These residues contribute to the polarity of the extension and promote its disorder, ensuring...

Ngày tải lên: 07/03/2014, 04:20

... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... properties and structure function relationships of USP7 were characterized We have mapped sites for phosphorylation and ubiquitination, and studied the oligomerization state of the enzyme in vitro and ... Switzerland) and Markus Schirle, Manfred Raida, Anne-Marie Michon and Sonja Ghidelli (Cellzome AG, Heidelberg, Germany) for technical support, Rita Schmitz (Novartis, Basel, Switzerland) for providing...

Ngày tải lên: 07/03/2014, 05:20

... vitro (i.e T56 and ⁄ or T58) [23] Functional complementation under standard growth conditions and under environmental stress Our analysis of the role of the two threonines for eEF2 function in yeast ... plasmids for transformation of Escherichia coli Gene 57, 267–272 48 Weisberg RA, Enquist LW, Foeller C & Landy A (1983) Role for DNA homology in site-specific recombination The isolation and characterization ... T56V and T58V The ability of the eEF2 mutants to functionally complement wild-type eEF2 was analysed by transforming GB1 cells (Table 1) with expression vector pCBG1202 coding for mutant forms...

Ngày tải lên: 07/03/2014, 05:20

... 6-phosphate, and activity was determined by decrease in absorbance at 340 nm For discrimination between the chloroplast and cytosolic isoforms, 25 mm NaH2PO4 was added to the assay [31,32] For measurement ... sequences determined from the purified protein For SoPFK1, the peptide fragments EIYFEP and GNAVLG were selected For SoPFK2, TIDNDI and YIDPTY were used for primer generation Degenerate oligonucleotide ... penalties and weight matrix choice Nucleic Acids Res 22, 4673–4680 Thollesson M (2004) LDDist: a Perl module for calculating LogDet pair-wise distances for protein and nucleotide sequences Bioinformatics...

Ngày tải lên: 07/03/2014, 11:20

... parent monomer Monomers A (green) and B (yellow) form dimer 1, while monomers C (red) and D (blue) form dimer L5 ⁄ 7, the loop between b-strands and which contains b-strand 6; N term, amino terminus ... to form tetramers, the next level of structure Modeling indicates that tetramer formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and ... bovine a-crystallin to substrate ratio for protection against dithiothreitol induced denaturation ranges from : for insulin and a-lactalbumin, : for BSA and 10 : for ovotransferrin, with the ratio...

Ngày tải lên: 07/03/2014, 12:20