in vitro studies a cellular techniques

... treatment for an inconsequential finding detected through breast cancer screening Abbreviations ADH: atypical ductal hyperplasia; ALH: atypical lobular hyperplasia; DCIS: ductal carcinoma in-situ; ... (review) Int J Oncol 2014;45:1785 –92. 21 Cancer CPA: Organized Breast Cancer Screening Programs in Canada: Report on Program Performance in 2007 and 2008 In Canadian Partnership Against Cancer; ... breast cancer or neoplastic precursor lesion in studies by thoroughness of pathology examination 3a: Invasive cancer 3b: In-situ cancer 3c: Atypical Hyperplasia Data points are proportional to

Ngày tải lên: 06/08/2020, 03:26

... metastases counted Statistical analyses The Kruskal-Wallis (KW) test, a nonparametric analog of analysis of variance, was performed to test for an overall difference among groups for Luciferase ... neutrophils was determined by FACS analysis using PE-conjugated anti-CCR2 from R&D Systems, Minneapolis, MN Analysis of the ability of less aggressive PyMT breast tumors in the mammary Fat Pad to ... progression and metastasis, data from multiple labora-tories indicate that inhibiting CCL2 will alter the tumor microenvironment and antagonize tumor growth The capacity of CCL2 to attract tumor-promoting

Ngày tải lên: 20/09/2020, 01:16

... of caspase 3 in all cell lines tested Similarly, activa-tion of caspase-7, as indicated by the appearance of a 20 kDa band, was observed in all BT treated cell lines As compared to all cell lines, ... in triplicate Data was expressed as mean ± SD of triplicate experiments Caspase 3/7 assay (apoptosis assessment) Caspase 3/7 activity was measured using Caspase-Glo 3/7 assay kit from Promega, ... lysates of treated and untreated cells (control) was carried out by PAGE and western blot analysis as described (Materials and Methods) Primary antibodies against activated caspase-3, caspase-7

Ngày tải lên: 05/11/2020, 01:03

... oxy hóa của cao chiết Huyết giác Dracaenae cambodianae Pierre ex Gagnep và Bồ kết (Gleditsia fera (Lour.) Merr.)  Định lượng một số hợp chất trong cao chiết Huyết giác Dracaenae cambodianae Pierre ... –pentahydroflavonone và (-) – epicatechin Cũng trong năm đó, 2 ellagic acid glycoside từ gai của G sinesis LAM cũng được tìm thấy là 3 – O – methylellagic acid – 4’ acetyl) – alpha – L – arabinofuranoside ... VIÊM IN VITRO CỦA CAO CHIẾT HUYẾT GIÁC (DRACAENAE CAMBODIA PIERRE EX GAGNEP) VÀ BỒ KẾT (GLEDITSIA FERA (LOUR.) MERR.) Trang 2ĐẠI HỌC QUỐC GIA THÀNH PHỐ HỒ CHÍ MINHTRƯỜNG ĐẠI HỌC BÁCH KHOA KHOA KỸ

Ngày tải lên: 16/10/2021, 08:05

... fi Ala, Asn555fi Ala, Glu667fi Ala, Phe692fi Ala, Phe700fi Ala, Phe718fi Ala, Val736fi Ala and Phe692fi Ala ⁄ Phe718 fi Ala Cell-staining experi-ments showed that none of these mutations significantly affects ... spanning the entire DG gene, including its signal peptide, and carrying the mutations analysed in vitro, such as Trp551fi Ala, Phe554fi Ala, Asn555 fi Ala, Glu667 fi Ala, Phe692fi Ala, Phe700fi Ala, ... recombinant pep-tides spanning the C-terminal domain of a-DG and the b-DG ectodomain by solid-phase binding assays and SPR, demonstrating that despite the intrinsic pit-falls of solid-phase binding

Ngày tải lên: 16/03/2014, 12:20

... Trang 1cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities Vero´nica A Lombardo, Pablo Armas, Andrea M J Weiner and Nora B Calcaterra ... by autoradiography Radioactive bands were quantified and annealing percentage was determined as indicated in Experimental procedures npGST-CNBPWT and npGST-CNBPS158A as well as pGST-CNBPS158A failed ... 2 min, at the indicated accurate molar amounts To start the reactions, the prein-cubated probes were mixed and aliquots were taken at spe-cific time points as indicated Annealing reaction was

Ngày tải lên: 16/03/2014, 12:20

... form amyloid-b (Ab) The beta-site APP cleaving enzyme cleavage releases an APP ecto-domain leaving a 99-amino acid membrane spanning C-terminal fragment (CTF), C99 C99 then undergoes intramembranous ... c-3FLAG standard indicated by the arrow marked ‘c std’ Lane 2, lysate sample alone Note that a separation between c-3FLAG standard and e-CTF-3FLAG was achieved in lane 1. An uncharacterized anti-FLAG ... [10,11], and have aided in the finding of activity-modulating factors [12,13] These assays have shown that peripheral mem-brane proteins are not necessary for activity as car-bonate washing retains activity

Ngày tải lên: 16/03/2014, 23:20

... 1B), both in size, organization, and pre-dicted intra- and interdomain interactions Consistent with the above argument, we suggest that RNA fold-ing advantages in the P4–P6 domain make Fse.L1898 ... developing alternative gene therapy approaches Group I ribozyme-based mRNA repair may represent an interesting new approach in gene therapy by reprogramming RNA molecules carry-ing disorder mutations ... substitution, and affected individuals accumulate partially degraded oligosaccharides in the lysosome [25] No causal treat-ments are currently available for a-mannosidosis, and there is thus a need

Ngày tải lên: 23/03/2014, 11:20

... 662.2 Da (GalNAcAN-containing trisac-charide repeating unit) and m/z 663.2 Da (GalNAcA-containing trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both ... is another at least partially exposed cell surface antigen that appears to mediate invasion [7] Monoclonal and polyclonal antibody analysis of a variety of A salmonicida strains has shown that ... Li1and Eleonora Altman1 1 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada;2Institute for Marine Biosciences, National Research Council of Canada, Halifax,

Ngày tải lên: 23/03/2014, 13:20

... rate constant was then calculated for laboratory data using the corrected finalcontaminant concentration as follows: Trang 14where: Ci=initial contaminant concentrationCf,corr=corrected final ... the data,and an appropriate initial and final concentration was chosen The value used for the initialconcentration was the concentration present following the lag period; therefore, all ratecalculations ... biodegradation rate constant values of methanol 158 Trang 7as well as aquifer environments This project has been completed to demonstrate that in manycases, a large amount of data is available

Ngày tải lên: 28/03/2014, 19:20

... its fully active 66 kDa form (71 kDa in the recombinant insect-produced form) The appearance of an intermediate molecular form can also be seen as a 74 kDa protein Both C-terminal cleavages were ... resulting purified poly-peptide was characterized by western blotting, amino acid analysis and N-terminal Edman sequencing (data not shown) MS analysis showed that the isolated CT-peptide had a molecular ... for membrane tethering, thus insuring cell-surface anchor-ing [37] In other cases, the CT-peptide contains integ-ral transmembrane motifs affecting the sorting and recycling of furin and PC5⁄

Ngày tải lên: 30/03/2014, 08:20

... The gene that encodes the Gal4-AP2 transcriptional activator, which contains the Gal4 DNA binding domain and an activation domain from the AP2 transcription factor, was obtained from a Sc pombe ... was also able to support transcriptional activation (at least fivefold, as measured with a Phosphoimager) by the mammalian Gal4-AP2 transcrip-tional activator protein (lane 11), indicating that ... Trang 1Mediator is required for activated transcription in aEvelyn Tamayo, Giuliano Bernal, Ursula Teno and Edio Maldonado Programa de Biologia Celular y Molecular, Facultad de Medicina, Instituto

Ngày tải lên: 30/03/2014, 14:20

... phosphotriesterase variants from a microbead-displayed library using in vitro compart-mentalization [17] In this study, we have investigated plasmid display [18] as an in vitro display format with general applicability ... acquiring SPR data for FLAG-PRAI and trPRAI binding to mAb M2, it became apparent that the latter displayed increased binding at any given concentration (Fig 6) Affinities for the antibody were quantified ... greater discriminatory power of plasmid A B C Fig 7 Biophysical characterization of the PRAI subdomain (A) Thermal denaturation of PRAI and trPRAI-His as monitored by CD at 219 nm Raw data and

Ngày tải lên: 30/03/2014, 20:20

... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin ... I kappa B kinase-beta: NF-kappa B activation and complex formation with I kappa B kinase-alpha and NIK Science 278, 866869 30 Rothwarf DM, Zandi E, Natoli G & Karin M (1998) IIKK-gamma is an ... function in gepasi The mathematical model described here has been submitted to the online Cellular Systems Modeling Database and can be accessed at http://jjj.biochem.sun.ac.za/database/ihekwaba/ index.html...

Ngày tải lên: 16/03/2014, 11:20

... 5¢-AAACCAGCCATGAATGAAAT-3¢) and with actin primers (forward primer: 5¢-GTTGGGAT GAACCAGAAGGA-3¢; reverse primer: 5¢-GAACCA CCGATCCAGACACT-3¢) as a control Reactions with no DNA added served as a ... promoter fragment The sequences of the oligonucleotides used for these experiments are: Oligo I, 5¢-CAAGGACGTTCGATGCA CTTCCAAAAAACATATAAT-3¢; Oligo II, 5¢-CAAT GTAGTATTAAAAAGTAGTAGTTAAAAGC-3¢; Oligo ... light-regulated gravitropism Planta 199, 18–24 20 Takezawa, D., Ramachandiran, S., Paranjape, V & Poovaiah, B.W (1996) Dual regulation of a chimeric plant serine/threonine kinase by calcium and calcium/calmodulin...

Ngày tải lên: 18/03/2014, 01:20

... guidance (49) An aspirating needle is introduced through a guide attached to a transvaginal probe and is inserted into first one ovary, then the other, via the vaginal fornices Almost all aspiration ... heterogeneity, increased wall breadth, and a more gradual transformation at the fluid– follicle wall interface Atresia is characterized by thin walls, high numerical pixel value (bright) signals, and highly ... were done among the many studies and measurement techniques have played a role in our seeming inability to interpret the data Consensus among studies is that implantation may occur as long as the...

Ngày tải lên: 05/08/2014, 16:20

... Furthermore, a significant increase in cleaved PARP protein was measured following combined genistein and radiation treatment, indicating increased apoptosis The authors proposed a mechanism of increased ... 70:161-169 Akimoto T, Nonaka T, Ishikawa H, Sakurai H, Saitoh JI, Takahashi T, Mitsuhashi N: Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: ... several intracellular and extracellular pathways including cell cycle control, apoptosis, and angiogenesis [12-14] In PC cell lines genistein incubation proved to have many effects in vitro Among...

Ngày tải lên: 09/08/2014, 09:22

... Nemeckova S, Hainz P, Otahal P, Gabriel P, Sroller V, Kutinova L: Early gene expression of vaccinia virus strains replicating (Praha) and non-replicating (modified vaccinia virus strain Ankara, MVA) ... glutinin viral Cellularprotein localization of the influenza virus haemagCellular and viral localization of the influenza virus haemagglutinin protein Vero cells were infected with MVA-HANP and HA ... (CRL-1573) Rat/small intestine; normal Hamster syrian/kidney; normal Human/colon; colorectal adenocarcinoma Rat/liver; hepatoma Human/small intestine; normal Human/duodenum; adenocarcinoma African Green...

Ngày tải lên: 12/08/2014, 04:21

... Nemeckova S, Hainz P, Otahal P, Gabriel P, Sroller V, Kutinova L: Early gene expression of vaccinia virus strains replicating (Praha) and non-replicating (modified vaccinia virus strain Ankara, MVA) ... glutinin viral Cellularprotein localization of the influenza virus haemagCellular and viral localization of the influenza virus haemagglutinin protein Vero cells were infected with MVA-HANP and HA ... (CRL-1573) Rat/small intestine; normal Hamster syrian/kidney; normal Human/colon; colorectal adenocarcinoma Rat/liver; hepatoma Human/small intestine; normal Human/duodenum; adenocarcinoma African Green...

Ngày tải lên: 12/08/2014, 04:21

... stained with intracellular cytokines by using PE-labeled anti-human IL4 mAb, PerCP-labeled anti-human IFNG mAb, APC-labeled anti-human IL10 and FITC-labeled anti-human IL17 mAb for 30 minutes at room ... Connecting lines indicates data obtained in the same subject before and after SIT treatment Medians are shown by horizontal dashed lines Statistical analyses were performed by Wilcoxon matched pairs ... of VAS (A) scores before SIT and after SIT in AR patients Connecting lines indicate data obtained in the same subject Statistical analyses were performed by Wilcoxon matched pairs sign rank test...

Ngày tải lên: 23/09/2015, 14:47