imaging of embryonic stem cell migration in vivo

... Supplementing Lefty2 RNAi cultures with recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was ... in maintaining the undifferentiated cell state (Figure 2) One interesting factor identified from the screen is Lefty2, a secreted protein well known for its role in regulating Nodal signaling ... Discussion Trang 22.1 ES cell secretion of LEFTY2 maintains pluripotency 2.1.1 Introduction The molecular basis of self-renewal and the maintenance of pluripotency in ES cells have yet to be fully...

Ngày tải lên: 11/09/2015, 16:02

... were maintained on uncoated cell culture dishes in ES cell medium containing DMEM/20% ES FBS (Invitrogen, Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), ... day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, was carried out using a commercial ES Cell ... primers contained a V5 tag fused at the C-terminal of Lefty2 for detection in western blots A V5 tag at the C-terminus of Lefty2 was chosen instead of the N-terminus because tagging at N-terminus may...

Ngày tải lên: 11/09/2015, 16:02

... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene ... M T., King, C C., and Hayek, A (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, ... LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables...

Ngày tải lên: 11/09/2015, 16:02

... plethora of differentiated cell types of the three germ layers in culture Another defining feature of ES cells is their acquired capacity to proliferate indefinitely in vitro without undergoing senescence ... understanding of the molecular mechanism underlying self renewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cell self renewal Despite the importance of stem cell self ... member of the HMG-domain DNA binding protein family It has been demonstrated that Sox2 has a role to play in maintaining epiblast pluripotency, as gene targeting used to inactivate Sox2 in the...

Ngày tải lên: 11/09/2015, 16:02

... retinoic acid induced differentiation of F9 cells Zfx • Zinc finger protein • Transcription factor • Link to canonical Wnt pathway • High expression in ES cells a-catenin • Subunit of ... -catenin (Catnb) • Subunit of Cadherin protein complex • Intracellular mediator of canonical Wnt signaling • Expression detected in ES cells Dot1l • Non-SET domain protein • Histone H3 methyltransferase ... • Zinc finger protein • Expression detected in ES cells and F9 murine teratocarcinoma • Differential expression pattern between ES cells and differentiated cells • Reduced expression during...

Ngày tải lên: 11/09/2015, 16:03

... remodeling Since hMSCs have the capacity to inhibit scarring and suppress T-cell activity, we investigated the potential of using hMSCs to reverse airway inflammation in the murine ovalbumin model of ... number of experiments including all of the animals in each of the different groups In some instances a description identifying “n” versus the numbers of ani-mals in each group is explained for ... of inflammation the hMSCs con-tinue elsewhere Profiling the life of hMSCs in vivo in the presence and absence of inflammation is the focus of on-going studies in our laboratory These results...

Ngày tải lên: 11/08/2014, 03:20

... regulation in Panc1 cells in vivo We assessed the effect of Six1 inhibition in Panc1 cells in vivo in a xenograft model For this analysis, shRNA-transfected Panc1 cells were used, showing a stably ... Table S1) Inhibition of Six1 impairs cell migration in vitro Expression of Six1 was inhibited transiently by siRNA in Panc1 cells and BxPc3 cells This resulted in a decreased expression of Six1 ... Nonspecific binding was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Inc., Burlingame, CA, USA) After antigen retrieval, slides were placed in an automated staining machine (DAKO...

Ngày tải lên: 19/09/2020, 21:41

... found in 5 of the 17 cases (29.4%) in the NACRT group and in 9 of the 11 Figure 1 Immunohistochemical staining of each CSC marker in pancreatic adenocarcinoma The arrows indicate strong staining intensity ... re-expression of CD133 protein in colorectal cancer cell lines in vitro and in vivo Lab Invest 2012, 92(11):1607 –1622. 39 Chute JP, Muramoto GG, Whitesides J, Colvin M, Safi R, Chao NJ, McDonnell DP: Inhibition ... on the basis of clinical ex-aminations and imaging studies Time to death, final follow-up examination, and the diagnosis of recurrence was measured from the date of surgery Surviving pa-tients...

Ngày tải lên: 14/10/2020, 15:52

... Protein Array Analysis of the Acellular Kidney The goal of decellularization was to retain ECM proteins post decellularization including focal adhesions containing cytokeratins and integrins We ... elastin fiber structure remained intact (B) H&E staining shows no cells remain on the ECM, and the architecture of the kidney matrix remains intact (lower right) Laminin and Fibronectin were ... developmental stage of the therapeutic cells, whether it be a stem cell or a fully mature renal cell, are important for delineating the role of acellular ECM in regenerative medicine studies Stem and pro-genitor...

Ngày tải lên: 19/11/2022, 11:43

... Profiling human embryonic stem cell differentiation <p>Transcriptional profiling of human embryonic stem cells differentiating into blast cells reveals that erythroblasts are the predominant ... understanding mixed populations of progenitor stages will be of increasing importance in stem cell research hESCs have been shown to be able to differentiate into a vari-ety of cell types, including ... first demonstrated using an in vitro differentiation sys-tem of mouse ESCs Replating of embryonic bodies (EBs) of mouse ESCs resulted in the formation of blast colony forming cells (BL-CFCs), which...

Ngày tải lên: 14/08/2014, 08:20

... Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency 6.1 Introduction LncRNAs are integral members of the ESC self-renewal regulatory circuit In one of the earliest studies of lncRNAs in ... 2008; Dinger et al., 2008) Recent studies in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) indicate that lncRNAs are integral members of the ESC self-renewal regulatory circuit ... evidence suggesting functional roles of lncRNAs in the brain includes a computational analysis of in situ hybridization data from the Allen Brain Atlas, which identified 849 lncRNAs showing specific...

Ngày tải lên: 09/09/2015, 17:54

... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell ... chromatin binding Quantitative PCR of the ChIP DNA revealed REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line (Figure ... expressed in rhabdomyosarcoma, a malignant cancer of the muscles More recent studies in mice models, however, uncovered a plausible role of Rmst in the brain In a study of Pitx3-GFP knock-in mice, in...

Ngày tải lên: 09/09/2015, 17:54

... extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells (hESCs), and human neuronal development, using hESCs as a cellular ... ectopically expressed in ReN-VM cells, and enrichment of RMST was observed in FLAG RIP, indicating physical association (B) Enrichment of RMST in SOX2 RIP also confirmed in vivo association of SOX2 and ... complex resulted in the loss of neurogenesis, indicated by decrease in the number of TUJ1+ and MAP2+ cells The similarity in loss-of-function phenotype, together with RNA- protein interactions,...

Ngày tải lên: 09/09/2015, 17:55

... applications of blind signal processing: (a) A multi-recording monitoring system for blind enhancement of sources, cancellation of noise, elimination of artifacts and detection of evoked potentials, ... identification of a mixing matrix H or its pseudo-inverse separating matrix W = H+ assuming only the statistical independence of the primary sources and linear independence of columns of H The mixing (ICA) ... knowledge of the mixing system Preferably, the inverse system should be adaptive in such a way that it has some tracking capability in nonstationary environments (see Fig.1.1) Instead of estimating...

Ngày tải lên: 10/12/2013, 14:15

... and n produces a binary rank operation of the binary input data (inside the moving window) For example, if r = (n+1)/2, we obtain the binary median filter whose Signals of a continuous variable ... (dashed line) and flat dilation f ⊕ B (solid line) (e) Opening f ◦ g (dashed line) and flat opening f ◦ B (solid line) (f) Closing f • g (dashed line) and flat closing f • B (solid line) The nonlinearity ... translation-invariant We call it a DTI system in analogy to linear time-invariant (LTI) systems that are shift-invariant and obey a linear (sum -of- products) superposition As an LTI system corresponds in...

Ngày tải lên: 16/12/2013, 04:15

... the available information At the beginning of bootstrapping, our system (init) scores 75%, but this improves to 79% after five iterations of reestimation (system) Most learning occurs in the first ... segmentation: Exploring the effects of context In In 46th Annual Meeting of the ACL, pages 398–406 Aria Haghighi and Dan Klein 2006 Prototype-driven learning for sequence models In Proceedings of the Human ... rather than several as in modified KN Input: vocabulary of surface forms u Input: C(u): candidate intended forms of u Output: intend(u): intended form of u foreach u ∈ vocab // initialization v ∗...

Ngày tải lên: 16/03/2014, 19:20

... recombined to give X : h using one of the CCG combinators in Section 2.2 The CCG category splitting procedure has two parts: logical splitting of the category semantics h; and syntactic splitting of ... Zettlemoyer, L S and Collins, M (2007) Online learning of relaxed CCG grammars for parsing to logical form In Proc of the Joint Conference on Empirical Methods in Natural Language Processing and Computational ... Learning Zettlemoyer, L S and Collins, M (2009) Learning context-dependent mappings from sentences to logical form In Proceedings of The Joint Conference of the Association for Computational Linguistics...

Ngày tải lên: 31/03/2014, 20:20

... published in France by Hermốs Science Publications in 2001 First published in Great Britain and the United States in 2006 by ISTE Ltd Translated by Antoine Hervier Apart from any fair dealing for ... the prior permission in writing of the publishers, or in the case of reprographic reproduction in accordance with the terms and licenses issued by the CLA Enquiries concerning reproduction outside ... Digital Signal and Image Processing using MATLABđ This page intentionally left blank Digital Signal and Image Processing using MATLABđ Gộrard Blanchet Maurice Charbit Part of this book adapted from...

Ngày tải lên: 08/04/2014, 10:06

... basic image processing techniques, where image points are mapped into a new value first by considering a single point in an original image, and then by considering groups of points Not only we ... computers offer the capability for a basic vision system, by including a camera and its interface within the system There are specialised systems for vision, offering high performance in more than ... set of pulses of uniform height) we obtain a set of pulses in the frequency domain, but spaced by the reciprocal of the time domain spacing This transform pair is actually the basis of sampling...

Ngày tải lên: 18/04/2014, 12:28

... input is the samples of a function or an image, we can exploit the relation of the adjacent scale coefficients to obtain all of the scaling and wavelet coefficients without defining the scaling ... the original data with less bits, the original data is said to have coding redundancy • We can obtain the probable information from the histogram of the original image • Variable-length coding: ... Transform Coding(1) Most of the information is included among a small number of the transformed coefficients Thus, we truncate or coarsely quantize the coefficients including little information...

Ngày tải lên: 08/05/2014, 18:42