identification and functional characterization of srnas in neisseria meningitidis

... residues Glu369and Asn381are associ-ated with increased affinity for L-ornithine, L-arginine, and L-lysine in hCAT-1, hCAT-2B, and hCAT-3, and a region of 80 amino acid sequences in length in hCAT members ... variety of membrane compart-ments, mediating the efflux and influx of molecules and ions and playing roles in osmotic and membrane poten-tial regulation in protozoans [22–24] The identification of ... curves were obtained by incuba-tion of induced and repressed yeast cells with increasing concentrations of [3H]-arginine or [14C]-ornithine sub-strates (Perkin-Elmer Reagents) The incubation time

Ngày tải lên: 02/11/2022, 11:34

... potentially involved in SECO glycosylation, 50 μg of crude recombinant protein for each of the 5 UGTs expressed in yeast was assayed with different aglycones including secoisloariciresinol, sillibinin, ... lignans in many plant species, including flax These enzymes sequentially reduce pinoresinol formed by the coupling of two molecules of coniferyl alcohol (Figure 1) in the presence of dirigent proteins ... 12 amino acids flanking the HCGW region of UGT74S1 and that of the sesame UGT71A10 (BAF96583.1) on one hand, and be-tween UGT74S1 and UGT94D1 (BAF99027.1) on the other hand was 75 and 42%, and

Ngày tải lên: 27/05/2020, 01:35

... synthase proteins, respectively, of algae and higher plants In addition, we investigated the effects of light stress on the expression of crtE and crtB in E gracilis Continuous illumination at 460 ... harvesting during photosynthesis and offer photoprotection for certain non-photosynthetic and photosynthetic organisms including cyanobacteria, algae, and higher plants Although, Euglenida contains ... (crtQ and crtH) [8–10] Euglenida contains β-carotene and xanthophylls such as zeaxanthin, diatoxanthin, diadinoxanthin and 9′-cis neoxanthin [8, 11–13], however, the biosynthetic path-ways and

Ngày tải lên: 26/05/2020, 20:40

... AccessTriterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica) Avinash Pandreka1,2†, Devdutta S Dandekar1†, Saikat ... azadiradione, and gedunin type of skeletons C- Seco triterpenoids have nimbin, salannin and azadirachtin type of skeletons Trang 3with transcriptome helps in analysis and identificationof genes involved in ... opening and further rear-rangements of C-ring thus producing nimbin, salannin and azadirachtin (6-15) type of skeletons (Fig 1) Although the biosynthetic pathway leading to the formation of triterpe-noids

Ngày tải lên: 26/05/2020, 22:00

... an-notation of the WRKY family in soybean and a functional analysis of some genes involved in response to P pachyr-hiziinfection Results Annotation andin silico characterization In total, 182 ... basis of the soybean defense against fungal infection and growth, identifying genes involved in susceptible or resistant response and char-acterizing their individual roles are key steps for engineer-ing ... variant and was shared by 11 genes Other atypical sequences, such as WRKYGEK, WRKYEDK, WKKYGQK, CRKYGQK and WHQYGLK, occurred in single proteins Nine WRKY proteins contained incomplete and/or amino

Ngày tải lên: 27/05/2020, 01:13

... study of protein-DNA interactions In this paper, we explored the genome-wide chromatin localization of ER-DNA binding regions by analyzing ChIP-Seq data from MCF-7 breast cancer cell line By integrating ... genes were found in the vicinity of ER binding sites Furthermore, we annotated the function of the nearest-neighbor genes of these binding sites using Gene Ontology (GO), KEGG, and GeneGo pathway ... binding sites (c) Comparison of the occurrences of ERE motifs between published and newly identified binding sites Table 3: Number of ER binding sites identified by three ChIP-Seq peak calling

Ngày tải lên: 02/11/2022, 11:34

... using agar plates containing 100 μg/mL kanamycin, followed by nucleotide sequencing of both strands to confirm in-frame insertion 2.6 Expression and Purification of Recombinant CcPrx4 Protein in ... shown in Figure 1A, the cDNA contained a 28 bp 5-untranslated region (UTR), a single open reading frame (ORF) of 741 bp encoding a peptide of 247 amino acids and a 115 bp 3-UTR, including a ... 1marine drugs ISSN 1660-3397 www.mdpi.com/journal/marinedrugs Article First Report of a Peroxiredoxin Homologue in Jellyfish: Molecular Cloning, Expression and Functional Characterization of

Ngày tải lên: 04/12/2022, 10:31

... Results and Discussion Development of PCR-based sexing method in G cornutus The sex of G cornutus is indistinguish-able during the larval periods, and it is also indistinguishindistinguish-able in ... bodies of unmated adult male and female G cornutus by dissecting a single leg, washing it in water, homogenizing it in 50 μ l of TES (0.1 M Tris-HCL (pH 9.0), 0.1 M EDTA, 1% SDS) and incubating ... morphology in both sexes In Gcdsx Figure 3 Alignment and phylogeny of GcDsx with other insect Dsx proteins (A) Alignment of the conserved 47 amino acid DM domain of Dsx proteins of five coleopteran

Ngày tải lên: 04/12/2022, 15:39

... been made in the identification and functional characterization of cassava genes and proteins in response to drought stress [16–19] However, very few studies concerning lncRNAs underlying cassava ... encod-ing 8-hydroxylase involved in ABA catabolism in cis-actencod-ing (Fig.5a), strongly indicating that TCONS_00060863 was a key lncRNA involved in ABA signaling pathway under drought condition In ... component of gibberelin (GA)-mediated signaling pathway [40] Interest-ing, a homolog of WRKY75, together with GA1 involved in GA biosynthesis and PHT1;7 related to Pi transport and specifically induced

Ngày tải lên: 06/03/2023, 08:51

... determined, and are shown in Table 1 In the transamination con-version of amino acid to a-ketoglutarate, Kmand Vmax values for d-Asp and d-Ala were 2.3 and 1 mm, and 2.5 and 5.0 lmolÆmin)1Æmg)1 ... d-enantiomers, acting on a variety of d-amino acids, including d-isomers of branched amino acids (Fig 6) The amino acid residues presumed to be involved in substrate recognition and binding are not ... and functional characterization of a d-amino acid aminotransferase in eukaryotes The results presented here provide important information for understanding the significance of d-amino acids in

Ngày tải lên: 30/03/2014, 04:20

... arabinose All 135 isolates except one fermented lactose, ribose and hydrolysed arginine, respectively Among the 135 streptococci investigated 94 and 4 of the strains fermented inulin and raffinose, ... observed, indicating that a wide variety of S uberis strains might infect and cause mammary gland infection due to the contamination of the gland from the environment This high degree of heterogeneity ... blood agar within the zone of staphylococcal α-toxin could be observed for five of the 130 S uberis strains In lectin agglutination reactions, 43 of 130 S uberis strains exhibited agglutination reactions

Ngày tải lên: 07/08/2014, 17:22

... Asso-ciates (Birmingham, AL, USA) After washing and blocking with PBS containing 1% BSA for 30 minutes, serial dilutions of cultured B cells were added and incubated overnight at 37°C Before plating, cultured ... fixed in formalin 10%, and embedded independently in paraffin blocks or optimal cutting temperature (OCT) compound, and snap frozen in liquid nitro-gen-cooled isopentane Immunohistochemical labeling ... panel) and PB (right panel) is shown. Trang 6Immunohistochemical staining for IgG, IgA, and IgM in synovialtissue sections showed a predominance of IgG-secreting cells Lower proportions of IgA- and

Ngày tải lên: 09/08/2014, 14:22

... of the binding sites of the AP2-domain containing transcription factors as inferred from protein-based microarray studies conducted in P falciparum [28] In a study of the promoter strengths of ... intriguing results Genes involved in nucleotide biosynthesis and salvage Purines and pyrimidines are the building blocks of nucleic acids in living cells All protozoan parasites examined thus far are ... occurrences of each candidate motif within coding and upstream regions Upstream Motif Number of genes Number of motifs Coding Number of motifs/10 kb Number of genes Number of motifs Number of motifs/10...

Ngày tải lên: 14/08/2014, 21:20

... science and directionality of projects His broad scope of scientific interests, inspiring ideas, critical thinking, deep penetrance of scientific investigations and many other outstanding scientific ... proteinRNA complexes with the RNA-induced silencing complex (RISC) and bind to either 3’UTR or coding regions of messenger RNAs, causing destabilization of mRNA and/ or inhibition of protein translation ... As shown in Fig 1.2, Ago are large proteins about 100kDa comprising a single     variable N-terminal domain and three conserved C-terminal domains, including the PAZ, MID and PIWI domains (Vaucheret,...

Ngày tải lên: 09/09/2015, 10:17

... VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction ... variant A Fig Interaction of Vpr and VBARP proteins in vitro and in vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK...

Ngày tải lên: 07/03/2014, 21:20

... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P-450scc Effect of cholesterol, ... was in the range of values determined in previous studies for the interaction between bovine Adx and bCYP11B1 Biacore measurements were performed to investigate the binding behavior between bovine ... transfer chain consisting of a [2Fe-2S] ferredoxin, adrenodoxin (Adx) and a NADPH-dependent, FAD containing reductase, adrenodoxin reductase (AdR; EC 1.18.1.2) [8,9] This electron transfer chain is...

Ngày tải lên: 30/03/2014, 04:20

... in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin [30–35] As Ig-C2 domains may serve as molecular spacers and bind to a diversity of ligands, ... domain in myosin light chain kinase A Fig Prediction for a conserved aggregation domain in the 4Ig region of L-MLCK and recombinant expression of MLCK variants (A) The sequences of the N-terminal ... barrier in intestinal disease Lab Invest 84, 282–291 12 Tran QK, Watanabe H, Zhang XX, Takahashi R & Ohno R (1999) Involvement of myosin light-chain kinase in chloride-sensitive Ca2+ in ux in porcine...

Ngày tải lên: 30/03/2014, 04:20

... results again highlight the stimulatory role of P2Y12 in mitogen-activated protein kinase and Akt kinase signaling pathways On the other hand, the functional activity of the P2Y1 receptor in long-term ... to fragmented adhesion region in the cell center Vinculin staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) ... chemiluminescence Equal loading of proteins was ensured by b-actin concentration Control: cells were growing in MEM containing 10% fetal bovine serum 24, 48, 72, 96 h: cells were cultivated in MEM...

Ngày tải lên: 30/03/2014, 08:20

... distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci In contrast, ... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... profound effect in the cell type-specific functions of Trip10 Discussion Trip10 was initially identified as a Cdc42-interacting protein involved in GLUT4-mediated glucose uptake in adipocytes and...

Ngày tải lên: 10/08/2014, 05:21

... Expression of recombinant HCT in E coli Expression of recombinant HCT in E coli Protein content of non-induced (T = h) and induced (T = h) non-transformed cells (1) were compared with those of non-induced ... quantity of HCT transcript and the content of caffeoylquinic acids was observed in all accessions Since HCT silencing induces an increase (or no significant change) in the amount of caffeoylquinic ... choleretic, diuretic and bile-expelling activities [4], (iv) the inhibition of HIV integrase, a key player in HIV replication and its insertion into host DNA [14,15], and (v) antibacterial and antifungal...

Ngày tải lên: 12/08/2014, 05:20