graphical analysis of loaded regulator

... 4E-BP1 (in place of its own TOS motif), as the mTOR regulation of 4E-BP1 is much better characterized than the control of STAT3 or PKC isoforms We therefore created a range of mutants of 4E-BP1, in ... a comparison of the amounts of GST fusion proteins used in each case Some degradation of the GST fusion proteins is evident from the presence of products running at the position of GST itself ... H Y Lee et al Regulatory motifs in 4E-BP1 A C B D Fig Analysis of the binding of raptor to variants based on 4E-BP1 (A) Schematic diagram of 4E-BP1 showing the RAIP and TOS motifs, the region...

Ngày tải lên: 16/03/2014, 06:20

... DEVELOPMENTAL ANALYSIS OF INTERFERON REGULATORY FACTOR (IRF6) GENE IN ZEBRAFISH BEN JIN (M.Sc., National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... knockdown 3.3 Phenotypes of Loss of irf6 function 82-83 88 vii LIST OF FIGURES Fig No 1.1 Page No Flowchart of characterization of irf6 using zebrafish model 31 2.1 Amplification of irf6 cDNA from AB ... 2.1.4 Studies of craniofacial development using zebrafish The head is composed of the brain and the organs of sight, hearing, taste, and smell Skull is the bones of the head, composed of two parts:...

Ngày tải lên: 14/09/2015, 12:44

... coelicolor, of a gene(s) encoding an iron -regulator of the DmdR family We report, in this article, the presence of two different genes – dmdR1 and dmdR2 – in the genome of S coelicolor, both of which ... reactions of iron metabolism (e.g siderophore biosynthesis) or may be regulatory proteins that respond to DmdR1 In summary, the important role of the DmdR1 regulator, but not of the DmdR2 regulator ... analyzed by using the mascot software [29] F J Flores et al Immunodetection analysis of DmdR1 and DmdR2 Western blot analysis of DmdR1 and DmdR2, after SDS ⁄ PAGE resolution of the proteins, was performed...

Ngày tải lên: 16/03/2014, 18:20

... which in turn is a function of the concentrations of these regulatory proteins This autocatalytic effect caused by the feedbacks of the regulatory proteins on the switching of the GAL genes is termed ... importance of the autoregulation of regulatory proteins and parametric sensitivity Subsequently, we considered a KlGAL80 mutant strain of K lactis to determine the importance of the autoregulation of ... operation of the GAL switch The steady-state model has been evaluated for regulatory designs of the GAL system It is of interest to ascertain the role of autoregulation in the synthesis of activator...

Ngày tải lên: 29/03/2014, 09:20

... for the alignment of genomic sequences, data visualization and the identification of exons [8,9] Algorithms for the analysis of regulatory sequences have addressed the detection of over-represented ... modular software for the integration of binding-site predictions with analysis of sequence similarity We show that our approach results in an increased specificity of predicted TFBSs as a result of ... significant reduction of noise The ConSite algorithm is thus particularly suited to the analysis of pairs of orthologous genomic sequences with limited or no experimental annotation of regulatory elements...

Ngày tải lên: 06/08/2014, 18:20

... http://genomebiology.com/2011/12/2/R17 of (p)ppGpp in E coli Of the many detected (p)ppGpp targets that may contribute to the observed stress phenotypes of the rsh mutant, we performed a phenotypic analysis of three specific ... (p)ppGpp-dependent regulators, providing added detail to the complex picture of the role of (p)ppGpp in R etli Results and Discussion Experimental design of the transcriptome analysis Previously, ... insertional activity and mobility of IS-related elements Other processes The impact of (p)ppGpp as a global regulator of transcription is further illustrated by its control of genes involved in diverse...

Ngày tải lên: 09/08/2014, 22:23

... regulating the expression of a diverse set of genes in many different tissues and stages of development Here, we present the results of our first large-scale analysis of the REDfly CRMs to define ... content of the REDfly analysis CRMs as well as coding, intronic, and GC content of the REDfly analysis CRMs as well as coding, intronic, and intergenic sequences terms of both fraction of aligned ... behaviors of the two types of functional sequences appear to Figure conservation properties of the REDfly analysis CRMs Sequence (see following page) Sequence conservation properties of the REDfly analysis...

Ngày tải lên: 14/08/2014, 07:21

... Number of IMCs, EPMs, RMs and their average number of member genes and regulators Average no of genes/transcription factors Condition No of survived IMCs No of EPMs No of RMs (average number of RMs ... reorganization of the transcriptional regulatory modules entail the alteration of targets and partner regulators of transcription factors, suggesting that rewiring of the transcriptional regulatory ... of multiple RMs appeared to represent a functional part of an EPM We will discuss the functional enrichment of RMs in detail later in the paper Overall results of module analysis The module analysis...

Ngày tải lên: 14/08/2014, 08:20

... proportion of pairs of regulators participate in multiple transcriptional programs Design principles of gene regulatory networks Systems biology can be regarded as the application of engineering principles ... factor binds different sets of promoters under different conditions In fact, more than 40% of the transcriptional regulators investigated were found to alter their set of target genes in an environmentspecific ... with other regulator proteins [10] Thus, the regulation of gene expression in a context-dependent manner may rely, to a large extent, on the combinatorial action of transcriptional regulators...

Ngày tải lên: 14/08/2014, 14:21

... [1,2] for recent reviews of known cis-regulatory mutations and their significance) However, despite this growing awareness of the significance of evolutionary changes of this kind, most studies ... motif, we then evaluated the profile of over-representation of these binding motifs across the homologous sequences of 25 eukaryote species Significant overrepresentation of the binding motif from ... remodeling of the cis-regulatory architecture affected most of the genes involved (a possible scenario for the SOX2 and POU5F1 gene batteries in rodent) As previously reported in yeast [5], a loss of regulatory...

Ngày tải lên: 14/08/2014, 21:20

... limit of comfort zone shown in Fig was fixed at 90% relative humidity In summer, this humidity limit may be even enlarged to 95% under effect of a wind speed of 0.8 – 1m/s due to the effect of air ... Switzerland lack of specific survey on this issue ASHRAE [2] specifies an upper humidity ratio limit of 0.012 kgw/kgdry air That is based on avoiding condensation and mold growth in the ducts of HVAC ... The comfort zone for Hanoi proposed by Climate consultant software [3] (using comfort model of ASHRAE standard 55) gives that only 4.9% of total time in a year should be comfortable, which is extremely...

Ngày tải lên: 19/06/2015, 10:00

... ANALYSIS OF REGULATOR OF G-PROTEIN SIGNALING (RGS) FUNCTION IN GROWTH, DEVELOPMENT AND PATHOGENICITY OF MAGNAPORTHE GRISEA HAO LIU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... 1.2.3.1 The discovery of Regulator of G protein signaling (RGS) Studies of the desensitization of G-protein-coupled signal transduction have led to the discovery of a family of guanosine triphosphatase ... phenotype of TMT1398…………………………………….79 Figure 17 Cloning of RGS1 .83 xii Figure 18 Alignment of Rgs1 with fungal orthologs……………………………………84 Figure 19 Analysis of RGS domain of Rgs1 with...

Ngày tải lên: 14/09/2015, 09:13

Ngày tải lên: 14/09/2015, 09:24

Ngày tải lên: 14/09/2015, 09:39

Ngày tải lên: 14/09/2015, 09:53

... 86 Chapter Results: Detection of regulatory DNA – Theoretical considerations 89 5.1 Conservation of regulatory information 89 5.1.1 Conservation of regulatory DNA 89 5.1.2 ... site UTR untranslated region (of an mRNA) xii List of publications Gilligan P., and Venkatesh B.; Application of comparative genomics to the analysis of vertebrate regulatory elements Briefings ... alignment of sequences 3’ of the mouse and human oxytocin polyA signals 92 5.2 The alignment of sequence 3’ of the mouse and human vasopressin polyA signals 93 5.3 Apparent “blocks” of contiguous...

Ngày tải lên: 15/09/2015, 22:13

... Mutational Analysis of p14ARF 60 2.4.4 Analysis of mRNA expression of p14ARF 61 RESULTS IN HUMAN BREAST CELL LINES 62 2.5.1 Mutational analysis of p53 62 2.5.2 Gene amplification of hdm2 63 2.5.3 ... extraction 48 2.3.4 Mutational analysis of p53 49 2.3.4.1 PCR-SSCP Analysis 49 2.3.4.2 Direct sequencing 51 iv 2.3.5 Mutational Analysis of p14ARF 51 2.3.5.1 PCR-SSCP analysis 51 2.3.5.2 Southern ... Mutational Analysis of p14ARF 64 2.5.4 2.6 RESULTS IN PRIMARY INVASIVE BREAST CARCINOMA 2.4.2 2.5 55 2.4.1 2.4 Safety precautions in use of radioactive materials Analysis of mRNA expression of p14ARF...

Ngày tải lên: 17/09/2015, 17:20

... installation of RM2000 and GP2000 Please always uninstall old program versions before installing a new program release! 1.2 Unit dependency of Import/Export of RM2000 Export and Import of RM2000 ... upgraded or the validity of a time limited version has expired New RM2000/GP2000 users as well as existing RM7 users must get a licence file from Tdv or one of the support offices in order to run ... calculation is numerically extended to cover more exactly “creep of creep” effects ( it is possible to decrease number of time steps ) Extensions of British Standard live load calculation Added possibility...

Ngày tải lên: 06/09/2012, 15:18

... Ownership of the program and the documentation remain with TDV Austria Use of the program and the documentation is restricted to the licensed users Unlicensed use of the program or reproduction of the ... point’ arrow at the top of the screen and the insert symbol Accept the default name Define the Connection Points Click on the intersection point of the CL’s at the bottom of each beam in the position ... load sets and the two loading cases (e.g.: L.C is a UDL of 10kN/m from Elem to Elem 20 and L.C is a Settlement of 0.001m at the beginning of element 1200.) Choose AddCon and insert the ‘Additional...

Ngày tải lên: 06/09/2012, 15:55