Analysis of regulator of g protein signaling (RGS) function in growth, development and pathogenicity of magnaporthe grisea 2

Figure 17 A B MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTAKPGSRKSLL LQQHQQQKSTTESTNAAPDSKPQRAGGIFAYAAAALDRTLSESRNIRSRPSLNRLSVSVLPESTTTT SSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSL

Figure 16

Figure 17

A

B

MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTAKPGSRKSLL LQQHQQQKSTTESTNAAPDSKPQRAGGIFAYAAAALDRTLSESRNIRSRPSLNRLSVSVLPESTTTT SSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSLGTPPSQPYNDTDPSQPAPVHVQRLSNKMHQTS SRLLRMTDDDRPFTKDFKDLFATLIVSMLPLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPD PKDVARIVTTTTTTTFSMAKEMARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILD SFASRNGIQDRQIPEVVGSSLAQLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSD SLSDYRDGLTGVKMAPERKVNGKIHKDTFTGKAASEWLMDCCTTVDRREAVEIASLFVEYELIEA LQQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHGVARDSNT QRMDKILNDAALRLLFRENLRETHCEENLSFYLDVEEFVRLCRSAVRQAQLAQSSPANSAEAKKQ ALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQDSAMVETLQEVMALF EDAQNAVFKLMASDSVPKFLRSRRYEHLLKNYDFDSVSLESSPRVPERSQSRSQRL

C

Figure 18

Rgs1 1 MDDTSRPDQVF ITSTQGIDHNNKPT PSEVVNEKS A L SAHL S LPAP P TTA Cprgs-1 1 - FlbA 1 MPTISSTPLDQGSPPSSQ HHPSCHSHTST S PVS T A I SSSIISP TSP P TSS Sst2 1 -MV KNRT H L S NFSRTPNGLIF NDLKTV SIFLICLDLKEKKHSSD Rgs1 59 KPGSRKSLLLQQHQQQKSTTESTN -AAPDSKPQRA GIFAYAAAALDRTLSES

Cprgs-1 1 - FlbA 61 T TTANTTATTTTSFTG VIGSISRRNRRSFAALAREKTSNAL NLSAIGSTTS SLRT Sst2 51 T SFLLTAFTKHFHFTF YQEAIKAMG -QLELKVDMNTTCINVSYNIKPSLARHLL Rgs1 111 RNIRSRPSL RLSVSVLPESTTTTSSPRQP GGNNGSSSPEKKARNRASVSNLRLSSLG- Cprgs-1 1 - FlbA 121 TSSGSLSKHSRKPSQISTHEIAGVSPLTPPLSDESTSSNQSSPACIETSEVESQQS SGR

Sst2 106 TLFMSSKLLHTPQDRTRGEPKEKVLFQPTP GVAVLQKYVRDIGLKTMPDIL SSFNS K

Rgs1 170 TPPSQPYN TDPSQPAPVHVQRLSNKMHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSML

Cprgs-1 1 -MHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSLL

FlbA 181 RRHTLQRNSPIS QQLEAGFAALPSKMHQTSSRLLRMTEDDRPFTKDFMDLFSTLMVSLK Sst2 166 LFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNA D LPCLSSLLEYTNNDDTF Rgs1 230 PLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPDPKDVARIVTTTTTTTFSMAKEM

Cprgs-1 35 PLSAHRVRLTKVEHTFLSEDAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKDM

FlbA 241 -LDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEM

Sst2 226 TFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTF Rgs1 290 ARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILDS ASRNGIQDRQIPEVV

Cprgs-1 95 ARSICQRFLEARLIESADGKLQQVYTMKGSVWQLTPKGIAILDQFCSKNGIQQKQVAELI

FlbA 300 ARSVCQRFVDARFIEPVDGKALPIFPLKGALFQLTPKGINILQRFCQRNGITAR V DVL

Sst2 286 G SKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALF KTGLIVPVLLQPSRTDKKK

Rgs1 350 G S L QLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSDSLSDYR Cprgs-1 155 G PALTQLVSLERESSTDKLQTDRATMEVVFRRFTGLKGLNIKSN NAADSESLSDYSD

FlbA 360 ESPRNTMQLVNLERDTETDKLSHDRATIEVIFRRFA QDGPNVKSSISTSDSDSLSDYSN

Sst2 346 FQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLD SE

Rgs1 408 GLTGVKMAPERKVNGKIHKDTFTGK-AASEWLMDCCTTVDRREAVEIASLFVE ELIEA Cprgs-1 213 GLTGVKMAAERKIGGKTYKETFTGK-AATDWLMDCSTTVDRRETVEIASYFVEFGLMEC FlbA 420 GLVGVKMAKERKINDKIYMNTFTGK-AAVDWLMDCSTTIERRETVLIAELFVKYGLITML

Sst2 406 G SQDMLISSSNLNKLDYVLTDPG RYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKK Rgs1 467 QQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGS PRGRASEGDATHSATHRHG-VA Cprgs-1 272 QQDRAYTAQYPGSQLFQPTKHSIYQITPNG DLINGMNSRGRTSDAES PRDHK N KLP

FlbA 479 QEDKGYPQPDASIVVFQPSKYAIYGITERGQRVCG -WIARD SRETFYDNRGMP

Sst2 466 LIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGS YQLN HHN Rgs1 526 RDSNTQRMDKILNDAALRLLFRENLR ETHCEENLSFYLDVEEFV LCRSAVRQAQLAQ

Cprgs-1 332 RDSNTQRLEKILSDPALRLLFRENLR ETHCEENLSFYLDVDEFV QCKLAIRNAQKNP FlbA 532 KDSNNARLNHILQDPALRLLFREFLR FS CEENLSFYLDVSEFTAA H S K GAFKK Sst2 526 LRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKN

Rgs1 584 SSPANSAEAKKQALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQ

Cprgs-1 390 STTS -MDGIKEIMAQAYGIYNAFLAPGSPCELNIDHQLRSNLATRMTKAVGQ

FlbA 590 -ADAVRETLAAAYGLYNAFLAPGSPCELNIDHALRNSLASRMTKAVGD Sst2 586 LSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVES Rgs1 644 D AMVETLQEVMALFEDAQNAVFKLMASDS -VPKFLRSRRYEHLLKNYDFDSVSL Cprgs-1 441 D AMIDTLHEVTALFEDAQNAVFKLMASDS -VPKFLRTSKYESVLKNYDFDSVSY FlbA 637 D SMFKSLQEVVQLFEMAQTSVFKLMSSFTDEDQDSVPKFLRDPKYAVVLQEHD D IGA Sst2 646 FPA LKV RKLYPLFEIVSN MYRLMNNDS -FQKFTQSDVYKDASALIEIQEKC- Rgs1 698 ESSP -RVPERSQSRSQRL

Cprgs-1 495 P G -KGLDRSQSRSNRK

FlbA 697 P A SPTPAPVPERSMSRSA S

Sst2 -

Figure 19

Figure 20

A

HPH1 RGS1

B

WT rgs1 D

Figure 21

_

A

WT

B

Rgs1 OP

rgs1 D

Comple-mented

Figure 22

_

Figure 23

Figure 24

Figure 25

MG00990

MG03146

Figure 26

MG09618

MG03726

MG08735