gene delivery technology adeno associated virus

... nonviral gene delivery Arjen Sloots and Winfried S Wels Chemotherapeutisches Forschungsinstitut, Georg-Speyer-Haus, Frankfurt am Main, Germany Virus-based vectors have been the gene delivery ... in alternative, nonviral delivery systems for gene therapy [2,3] Although still less efficient than most viral vectors, nonviral gene delivery vehicles are not usually associated with serious safety ... unidentified target molecule HMGB2186facilitates gene delivery into COS-7 cells To investigate HMGB2186-mediated gene transfer, the pCMS-eGFP-E2C-Luc reporter gene plasmid was used which encodes enhanced

Ngày tải lên: 16/03/2014, 22:20

... understanding the impact of each of the lipid structural components on gene Keywords cationic lipid; elasticity; FRET; gene delivery; lipoplex Correspondence M Savva, Division of Pharmaceutical ... The development of highly potent and minimally toxic cationic lipids for nucleic acid delivery depends on generation of meaningful structure–activity relation-ships The rational design of efficacious ... Trang 1cationic lipids for gene delivery – synthesis, in vitrotransfection activity, and physicochemical characterization Michael

Ngày tải lên: 23/03/2014, 07:20

... Methods in Molecular BiologyTM TMEdited by William C Heiser Gene Delivery to Mammalian Cells VOLUME 245 Volume 1: Nonviral Gene Trang 2or gene delivery Synthetic compounds used to facilitate DNA transfer ... peptide-based gene delivery systems for use in plasmid-based gene therapy Biophys J 74, 2802–2814. 14 Mahat, R I., Monera, O D., Smith, L C., and Rolland, A (1999) Peptide-based gene delivery Curr ... Therapy Systems GenePORTERΤΜ DOPE
Proprietary compounds 888-428-0558 GenePORTERΤΜ2 Proprietary material www.genetherapysystems.com BoosterExpressΤΜReagent Kit Proprietary material PGene GripΤΜVector/Transfection

Ngày tải lên: 10/04/2014, 22:42

... transcription-polymerase chain reaction MWP: mealybug wilt of pineapple GLRaV-3: Grape leafroll associated virus TMV: Tobacco mosaic virus ELISA: Enzyme-linked immunosorbent assay TBIA: Tissue blot immunoassay ... nguồn chồi giống ban đầu không sạch các mầm bệnh, đặc biệt là virus gây bệnh đỏ đầu lá PMWaV (Pineapple mealybug wilt – associated virus) Giống dứa Trang 73 Cayenne nhập nội mẫn cảm với bệnh ... khái quát một trong những qui trình tạo chồi dứa sạch virus từ mẫu chồi ngoài đồng bị nhiễm virus PMWaV và bước đầu chứng minh hiệu quả loại trừ virus của phương pháp xử lí nhiệt kết hợp nuôi cấy

Ngày tải lên: 28/07/2014, 04:21

... 2.7.Bệnh héo do virus 13 2.8 Phương pháp tạo cây sạch virus 14 2.8.1 Sơ lược về hình thái, cấu tạo và sự di chuyển của virus thực vật 14 2.8.2 Cơ sở của các phương pháp tạo cây sạch virus 15 ... NGHỆ SINH HỌC NGHIÊN CỨU TẠO CÂY DỨA CAYENNE IN VITRO SẠCH VIRUS GÂY BỆNH HÉO ĐỎ ĐẦU LÁ (PMWaV- Pineapple mealybug wilt associated virus) CN LƯU PHÚC LỢI Trang 3LỜI CẢM ƠN Xin chân thành cảm ... Tháng 08/2005 “NGHIÊN CỨU TẠO CÂY DỨA CAYENNE IN VITRO SẠCH VIRUS GÂY BỆNH HÉO ĐỎ ĐẦU LÁ (PMWaV- Pineapple mealybug wilt associated virus)” Hội đồng hướng dẫn:  TS Trần Thị Dung  CN Lưu Phúc

Ngày tải lên: 28/07/2014, 04:21

... work is properly cited. Trang 2or gene-transfer vector constructs or target host genes,such as the gene for the CCR5 coreceptor [3-5] All lentivirus-based gene delivery systems contain packag-ing ... Lentivirus-based gene delivery systems have been used extensively for gene transfer into a variety of different tar-get cells, both ex vivo and in vivo [1] A possible application of lentivirus-based ... production of virus particles Conclusion: A simple modification of an 1 gene delivery system, namely, replacement of HIV-1 RRE with that of SIV, allowed efficient delivery of Rev MHIV-10 transgene into

Ngày tải lên: 10/08/2014, 05:21

... larger than that for avian influenza (Figure 3) Phylogenetic trees for the M gene Figure 1 Phylogenetic trees for the M gene Figures shows phylogenetic trees constructed using RAxML Scale bar shows ... populations When the H3N2 virus with the M gene in Hu1 acquires the M gene from H1N1 in Hu2, such a virus might not replicate and/or transmit effectively On the other hand, M genes of avian influenza ... [13,58-60] The M gene may be directly and/or indi-rectly linked to host tropism of the virus The evolutionary rate of the M gene was low in avian viruses compared to human and swine viruses (Figure

Ngày tải lên: 12/08/2014, 04:21

... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviral-oncogene transcript (69, 70) This suggests that the risk of oncogene ... retroviruses to be described were the oncogenic retroviruses Avian leukosis virus (ALV), and Rous sarcoma virus (RSV), which are both found in chick-ens A large number of oncogenic retroviruses ... onco-genic viruses, they are also associated with wasting syndromes Oncogenic retroviruses The archetypal retroviral pathogen is the oncogenic retro-virus Some of these are replication defective retroviruses

Ngày tải lên: 14/08/2014, 19:22

... gene delivery capabilities, including Herpes Simplex Virus (Maguire-Zeiss et al., 2001), Adeno-Associated Virus (Jooss and Chirmule, 2003), Adenovirus (St George, 2003) Retrovirus, and Lentivirus ... batch of virus production, or Adeno-associated virus which requires complicated process for virus purification However, when employing baculovirus as a gene delivery vector, especially for gene therapy ... Introduction……….1 1.1 General Introduction……….2 1.1.1 Gene Therapy……… 2 1.1.2 Non-viral and Viral Gene Delivery Systems………2 1.1.3 PEI and Its Role in Gene Delivery……… 3 1.1.4 Viral Gene Delivery to the

Ngày tải lên: 14/09/2015, 11:28

... most gene delivery experiments A variety of viral vectors, including adenovirus, retrovirus, Herpes simplex virus (HSV), AAV, and, more recently, baculovirus have been applied for gene delivery ... gene delivery to the CNS Three types of gene delivery methods, namely physical methods, non-viral methods and viral methods, have been applied for gene delivery to mammalian cells Physical delivery ... of gene therapy for neurodegenerative diseases Gene delivery of neurotrophic factors, mainly glial cell-line derived neurotrophic factor (GDNF), by viral vectors such as adenovirus, lentivirus

Ngày tải lên: 16/09/2015, 08:30

... to target certain cell types Retroviruses and adenoviruses (Ad) are the two most commonly used viral vectors for gene delivery Retroviruses confer stable transgene expression due to its propensity ... 27Table 1.1 Vectors and delivery systems for gene therapy Non-viral vectors • easy to produce • low gene transfer efficiency • transient gene expression Retrovirus • stable gene expression due to ... cause mutagenesis; • limited transgene capacity (~8kb) Adenovirus • transient high level of gene expression • infect dividing and nondividing cells • host immune response • limited transgene capacity

Ngày tải lên: 16/09/2015, 15:54

... such as retrovirus (Weber et al., 2001), adenovirus (McConnell et al., 2004), adeno-associated virus (Conlon et al., 2004), herpes simplex virus type 1 (Epstein et al., 2005), or lentivirus (Copreni ... 2005), among which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy Trang 14Suicide gene therapy is one of the commonly ... Trang 9AAV adeno-associated virus BBB blood brain barrier BLI bioluminescence imaging BV Baculovirus CAG CMV enhancer/β-actin promoter CMV Cytomegalovirus CMV E enhancer of cytomegalovirus immediate-early

Ngày tải lên: 01/10/2015, 11:39

... into cells for gene therapy Viral vectors including retroviruses, adenoviruses and adeno-associated viruses are particular attractive as vectors due to ease of production and generally high efficiency ... cell-specific gene delivery is to target vehicle binding to a cell-specific receptor through receptor-mediated endocytosis In this study, we present a non-viral gene transfer vector for targeted gene delivery ... TRANSGENE EXPRESSION MEDIATED BY NL4-10K 67 FIG 5-3: EFFECTS OF THE FORMULATION ORDER OF VECTOR TRIPLEXES ON THE GENE DELIVERY EFFICIENCY 68 FIG 5-4: SPECIFICITY OF NL4-10K MEDIATED GENE DELIVERY

Ngày tải lên: 27/11/2015, 11:11

... PE: Evaluation of adeno- associated virus- mediated gene transfer into the rat retina by clinical fluorescence photography Hum Gene Ther 1999, 10:641-648 Redmond TM, Hamel CP: Genetic analysis of ... improve adenovirus vector performance in vitro and in vivo Gene Ther 2000, 7:1624-1630 Malik AK, Monahan PE, Allen DL, Chen BG, Samulski RJ, Kurachi K: Kinetics of recombinant adeno- associated virus- mediated ... RPE65 dog model was generated by using recombinant adenoassociated virus (rAAV) to deliver and express normal, non-mutated RPE65 cDNA [16,22,23] The use of rAAV-mediated gene therapy has attracted...

Ngày tải lên: 14/08/2014, 19:22

... (2005) Adeno- associated virus vectors in clinical trials Hum Gene Ther 16, 541–550 Promoter and SUMF1 effect on Morquio gene transfer 34 Wu Z, Asokan A & Samulski R (2006) Adeno- associated virus ... Pandey K, Xu H, Feuss S, Storm TA et al (2008) The host response to adenovirus, helper-dependent adenovirus, and adeno- associated virus in mouse liver Mol Ther 16, 931–941 62 Chung S, Ma X, Liu Y, ... recombinant adeno- associated virus Gene Ther 5, 1642–1649 51 Toniatti C, Bujard H, Cortese R & Ciliberto C (2004) Gene therapy progress and prospects: transcription regulatory systems Gene Ther...

Ngày tải lên: 29/03/2014, 21:20

... by adenovirus-mediated gene transfer Hum Gene Ther 1999, 10:1667-1682 12 Jung SC, Han IP, Limaye A, Xu R, Gelderman MP, Zerfas P, Tirumalai K, Murray GJ, During MJ, Brady RO, Qasba P: Adeno- associated ... mouse model Among them, a recent gene therapy study using pseudotyped recombinant adeno- associated virus (rAAV) vector showed very promising results [13] Although the gene therapy studies should have ... regarding a comparison study of gene therapy and ERT In the present study, we investigated pseudotyped rAAV2/8-mediated gene delivery of α-Gal A and compared the efficacy of gene therapy with that of...

Ngày tải lên: 10/08/2014, 05:21

... it is essential to explore other gene delivery vectors for prolonged gene expression We sought to use adeno- associated viruses (AAV) for the longevity of transgene expression [4] Furthermore, ... recombinant adenoassociated virus vectors Hum Gene Ther 1998, 9:2745-2760 Conway JE, Rhys CM, Zolotukhin I, Zolotukhin S, Muzyczka N, Hayward GS, Byrne BJ: High-titer recombinant adeno- associated virus ... recombinant adeno- associated virus vectors J Virol 1996, 70:3227-3234 McCarty DM, Fu H, Monahan PE, Toulson CE, Naik P, Samulski RJ: Adeno- associated virus terminal repeat (TR) mutant generates...

Ngày tải lên: 14/08/2014, 19:22

... cells poorly, and adenoviruses produce an acute inflammatory response and an immune response to viral vector-encoded antigens [7,8] We therefore developed an adeno- associated virus (AAV) system ... dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis J Virol 2001, 75:3501-3508 Ponnazhagan S, Mahendra G, Curiel DT, Shaw DR: Adeno- associated virus ... 1997, 176:322-330 Bueler H: Adeno- associated viral vectors for gene transfer and gene therapy Biol Chem 1999, 380:613-622 Kay MA, Glorioso JC, Naldini L: Viral vectors for gene therapy: the art of...

Ngày tải lên: 14/08/2014, 19:22