dna immunoprecipitation medip based method medip chip

... HPV-16-positive human DNA samples were kept in the clinical laboratory of our department QIAamp® DNA Blood Mini Kits (Qiagen) were used to extract DNA according to the manufacturer’s protocol All DNA samples ... antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method BMC Infect Dis 2005, 5:53 27 Liu HH, Cao X, Yang Y, Liu MG, Wang YF: Array -based nano-amplification ... 14.5, any result under 14.5 from the 160 DNA samples was considered as positive one (Figure 3) Based on this cutoff value, all of the ten HPV-16-positve DNA samples were determined as positive...

Ngày tải lên: 21/06/2014, 01:20

... Biological Background: DNA and Sequence Features 1.2.1 DNA and Genomic Sequence 1.2.2 Regulatory Sites - a Feature of Genomic Sequence 1.3 Finding Sequence Features based on Sequence ... Constraint based Model 23 2.3 Motif Finding Algorithms 25 2.4 Significance of the Thesis Revisited 27 Finding Motif using Constrain Based Method 3.1 ... natural DNA is constructed Abstractly, a DNA sequence is simply a string over the alphabet {A,C,G,T} We will use the terms “string” and “sequence” interchangeably The sequence of bases of one DNA...

Ngày tải lên: 03/10/2015, 21:58

... second method is based on the Darcy’s law, which can be broadly classified into two types: laboratory test and field test The former mainly includes constant-head method and falling-head method, ... to present a novel laboratory method determining the hydraulic permeability of sand layer based on the capillary rise (CR) theory In nature, this method was also based on the Darcy’s law The tests ... sand layer based on the CR method 3.2 Data treatment The porosity φ was tested according to the national standard Soil Test Method (GB/T 50123-1999), and calculated by 100 ρ d (7) 1000G Based on...

Ngày tải lên: 05/09/2013, 17:03

... works Some of the methodological ideas of LEXTER are similar to those expressed in (Andreewsky et al., 1977) for the extraction and disambiguation of lexical sequences in a method of building ... to ambiguity resolution Many rule -based approaches have been proposed which require a huge amount of hand-coded knowledge (Jensen and Binot, 1987) proposes a method to disambiguate prepositional ... in the corpus We call this method Endogeneous since it does not need to integrate any domain-dependent, lexico- or syntactico-semantic information We called it Corpus -Based since it exploits the...

Ngày tải lên: 09/03/2014, 01:20

Ngày tải lên: 09/03/2014, 01:20

... disambiguation methods can be classified into two categories: the shallow methods and the knowledge -based methods Most of previous named entity disambiguation researches adopt the shallow methods, ... and discuss the performance of our method in Section 4.4 55 www.ldc.upenn.edu/Catalog/docs/LDC2006T13/ 4.1 network based methods, which is the same as the method described in Malin et al (2005): ... relatedness sr between Wikipedia concepts using the method described in Milne and Witten(2008): represent them using a graph -based model, semantic-graph Then based on the principle that “two concepts are...

Ngày tải lên: 16/03/2014, 23:20

... between the current methods in practice and methods that were developed based on scientific methodology Eventually, there is a lack of paper industry-specific post-completion audit methods This chapter ... developing the evaluation method First of all, the evaluation method developed for investments in a paper production line has to be practical It is a clear benefit if the method is based on a common ... evaluation methods are based on an economic approach, which can be divided into normative and behaviourist investment research (Jokisuu T 2002, p – 12) In these methods investments are ranked based...

Ngày tải lên: 18/03/2014, 02:20

... a subscript operator "." is used to extract a specified field of x, where x.fieldname = the value of filed fieldname x In the model, all the detectors form a Set Detector called SD SD = {< d ... average performance of Bayesian method and our model in the comparison experiment As indicated by the experiments, it can be concluded that artificial immune -based detection of spam can prove ... Laboratory The main aim of the experiment was to test the feasibility of the application for anti-spam based on AIS to implement spam detecting And we developed some series experiments Here are the coefficients...

Ngày tải lên: 22/03/2014, 22:26

... procedures We have developed a method which identifies coenzyme binding regions in proteins, and also predicts if the specificity is FAD, NAD or NADP The method is based upon a combination of HMMs ... 70000 Open Reading Frames (ORFs) Results and discussion We have developed a method for prediction of coenzyme specificity, based upon hidden Markov models (HMMs) and sequence motifs (see Experimental ... demonstrates the power of sequence -based predictions It is our hope and belief that the presented prediction tool will be a welcome addition to the arsenal of analysis methods available for large scale...

Ngày tải lên: 23/03/2014, 10:21

... our method on two set of bilingual news comparable corpora English-Chinese and English-Malay Experimental results show that 1) when only using Fourier Transform -based term frequency, our method ... Tao (2005)’s method by 4.1% and 8% for the top 100 alignment candidates and, 2) when using all features, our method significantly outperforms our implementation of Munteanu’s (2006) method by 23.2% ... literature, there are many methods concerning the estimation of the overall score for a given feature set, which vary from supervised to unsupervised method Supervised methods such as Support Vector...

Ngày tải lên: 24/03/2014, 03:20

... Chromosome I: b II: mtDNA insertion in region c III: e IV: a, c I: b, c, d, e II: b, mtDNA insertion in region c III: e IV: a, c V: a, b, c I: a, b, c, d, e II: b, d, e, mtDNA insertion III: a, ... identifying compositional domains in DNA sequences Bioinformatics 15, 974–979 15 Zhang CT, Wang J & Zhang R (2001) A novel method to calculate the G+C content of genomic DNA Sequences J Biomol Struc ... for visualizing and analyzing DNA sequences J Biomol Struc Dyn 11, 767–782 20 Zhang CT & Zhang R (2003) An isochore map of the human genome based on the Z curve method Gene 317, 127–135 3336...

Ngày tải lên: 30/03/2014, 20:20

... obtained from the manual method The results show the effectiveness of the method for diameter measurement The method is automated, accurate, and much faster than manual method and has the capability ... Nanoscale Res Lett (2007) 2:597–600 the method fails in measuring fiber diameter at intersections New Distance Transform Algorithm We established a new method based on image analysis in which the ... methods are shown in Fig It is apparently seen that fiber diameter distribution measured by new distance transform method is so close to the real distribution (simulation) Distance transform method...

Ngày tải lên: 22/06/2014, 06:20

... the proposed and the abovementioned sequence -based prediction methods At the same time, we note that the ML methods establish the prediction model based on information concerning both the seed ... NBmiRTar method in our experimental section We aim at developing a novel, traditional prediction method, named HuMiTar, which addresses some of the drawbacks of the existing seed -based methods ... approach, in which instead of filtering results of existing sequence -based methods (as done by the ML methods), we develop a novel sequence -based design that Page of 12 (page number not for citation purposes)...

Ngày tải lên: 12/08/2014, 17:20

... In this paper, we present an efficient method for the screening problem, which is based on an extension of an upper bound given in [6] and two novel tree based data structures for storing and ... method and tested it on a realistic data set Our experiments clearly demonstrate that it is superior to previous strategies, as it yields a three-fold speed-up over the previous best method Methods ... present a method for finding all fingerprints B in a database of fingerprints with a Tanimoto coefficient above some query-specific threshold Smin to a query fingerprint A The method is based on...

Ngày tải lên: 12/08/2014, 17:20

... axiomatic Whereas method is an overall plan for the orderly presentation of language material A method is procedural No part of a method contradicts and all of the method is based upon a selected ... language 10 I.3 Task -based Method the strong version of CLT Task based Teaching can be regarded as a strong and recent version of communicative methodology and seeks to reconcile methodology with ... Common methods in teaching listening Underwood (1990, p.90) states that there are at least four common methods of teaching L2 listening: Grammar-Translation Method, Grammar Method, Audio-Lingual Method...

Ngày tải lên: 28/03/2015, 08:50

... school by using the Task -based language teaching to improve reading skill for the students That is the reason why I choose to investigate the effectiveness of using task -based method in teaching and ... teaching in Reading class Chapter III, Methodology, presents the research methodology employed and the rationale of using qualitative and quantitative method for the achievement of the aim of ... suggestions for using Task -based language teaching to improve students’ reading skills? 1.4 Scopes of the study The study is concerned with investigating the effectiveness of using Task -based language teaching...

Ngày tải lên: 10/08/2015, 19:49

... algorithmic method consists of a large number of techniques which can be further divided into two classes: function based methods and machine learning methods Examples of function based methods are: ... regression based methods, dynamic based models, and composite methods Directly under each major class are the estimation methods and this system can include most types of estimation methods (Boehm ... Wieczorek (2002) defined a hierarchical scheme starting from two major classes (model -based methods, non- model -based methods) that are further divided into several sub-classes The sub-classes contain...

Ngày tải lên: 14/09/2015, 08:40

... classical methods are discussed including spectral -based methods and the maximum likelihood (ML) methods The spectral -based methods include beamforming techniques and subspace methods In these methods, ... the proposed method is derived in Chapter Several searching methods are studied including steepest descent method, Newton’s method, Gauss-Newton method and alternating minimization method At the ... Newton’s method, many techniques are developed based on Newton’s method 3.4.3 Gauss-Newton Method The Gauss-Newton method is suitable for small-residual non-linear least square problems It is based...

Ngày tải lên: 15/09/2015, 22:51