division of labour in eukaryotic cells

... number of cells was analyzed (Fig 2B) In order to investigate whether CEdG has the potential to accumulate in genomic DNA of nongrowing cells, HSC-T6 cells were kept in minimal medium containing ... detected in human urine [13], indicating the formation of DNA AGEs in the healthy human organism A significantly increased number of CEdGpositive cells were immunostained in glomeruli of patients ... we investigated whether CEdG accumulates in resting HSC-T6 cells After 14 days of incubation, a 1.7-fold increase of CEdG content in the genomic DNA of the cells was measured These results indicated

Ngày tải lên: 30/03/2014, 04:20

... nucleolin, suggest that the C-terminal tail of nucleolin should be implicated in the mechanism of binding of Lf to nucleolin In general, the crosslinking of a ligand leads to the clus-tering or ... the binding of HB-19 Similarly, the iron-binding hTf had no effect On the other hand, bLf inhibited the binding of HB-19 to cells In accordance with the involvement of both lobes of hLf in the ... nucleolin are involved in the endocytosis of hLf into CHO K1 cells Fig 7 Binding of human lactoferrin (hLf) to nucleolin expressed on MDA-MB-231 cells The figure shows the green fluorescence intensity

Ngày tải lên: 07/03/2014, 15:20

... critical in maintaining cir-culating glucose levels and, in a preliminary investigation using the human hepatoma (HepG2) cell line in this study, we demonstrated the role of TNFa in the regulation of ... prevention of the effect of insulin on Foxa2 localization and lead-ing to the abrogation of insulin inhibition of gluconeo-genesis and glucose output in HepG2 cells Although TNFa-mediated inhibition of ... validation in primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFa-mediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells

Ngày tải lên: 16/03/2014, 02:20

... Zinc, but not cadmium, treatment induces apoptosis in JPX-9 cells To examine the effect of Tax on the growth/death of Jurkat cells, we studied its induction by Zn or Cd in the JPX-9 cell line ... cause apoptosis in a Jurkat T-cell line, JPX-9 Tax expression can be induced equally by either cadmium (Cd) or zinc (Zn) in JPX-9 cells Surprisingly, when induced by Zn, but not when induced by Cd, ... efficiencies of Tax induction by the two cations, we directly examined the kinetics of Tax protein expression after Zn- or Cd- treatment The results indicated essen-tially no difference in Tax induction

Ngày tải lên: 13/08/2014, 13:20

... cell content from simulated data using segmentation Tumor cells (%) Tumor cells using hemizygous loss (%)* Tumor cells using single copy gain (%)† Tumor cells using copy neutral LOH (%)‡ Paired ... (Courtesy of Illumina Inc.) Reference data set consists of 120 HapMap samples hybridized on Illumina HumanHap300 version Genotyping BeadChips (Courtesy of Illumina Inc.) Reference data set consists of ... Illumina HumanCNV370 Genotyping BeadChips (Courtesy of Illumina Inc.) Reference data set consists of 120 HapMap samples hybridized on Illumina HumanHap550 Genotyping BeadChips (Courtesy of Illumina

Ngày tải lên: 14/08/2014, 20:22

... Effects of hypoxia on protein profiles in the presence/absence of p53 68 4.2.2 Gene ontology and protein-protein interaction analysis using Ingenuity Pathway Analysis (IPA) tool 5.2 p53 protein does ... dimerization and DNA binding while the PAS domain is highly conserved throughout evolution, consisting of two internal homology units (A and B repeats) which are involved in protein-protein interactions ... illustrating the domains of p53 36 1.8 Proposed model showing different levels of HIF-1-p53 interactions in the presence of hypoxia and anoxia 42 1.9 Different p53 isoforms and their mechanisms of

Ngày tải lên: 16/09/2015, 15:43

... DIP2B sharing 72.2% amino acid identity [6] All three proteins are predicted to contain DMAP1 binding (pfam06464) and AMP binding (pfam00501) domains, which give properties of binding to the ... following screening of 605 Geneticin-a b Fig 1 DIP2C knock-out by rAAV-mediated deletion of 48 bp in exon 9 in human cancer cells a Coding sequence and predicted structural protein domains of DIP2C ... large part of DIP2C exon 9 was deleted, possibly generating a truncated protein with a preserved DMAP1 binding domain which could cause binding of non-functional DIP2C and blocking of nor-mal

Ngày tải lên: 06/08/2020, 06:20

... observed inhibitory effects of SATB1 inhibition Our findings are also in line with a recent study in colorectal cancer, where SATB1 was found to be a target of Wnt/β-catenin signaling while in turn ... Survivin (Fig 3a) The latter finding correlated well with decreased protein levels of the anti-apoptotic protein Survivin as determined in Western blot experiments, with si467 showing the most profound ... gene_expression_in_glioma_subtypes/) did not support the notion of SATB1 overexpression in brain tumors In another study, initial results in one cell line indicated a possible role of SATB1 in some cellular

Ngày tải lên: 20/09/2020, 18:59

... selective inhibition of TCTP on the cleavage of procaspase-9 into p37 in etoposidetreated cells indicates that TCTP may not be involved in the initial auto-catalysis but inhibits the amplification of ... p53 ubiquitination [11] and reduce the intracellular contents of TCTP, thus promoting apoptosis in tumor cells [52] TCTPbinding antimalarial drug, artemisinin derivatives are being investigated ... by investigating whether TCTP involves in the development of chemoresistance in mitochondriamediated apoptosis We specifically examined the role of TCTP in apoptosome inhibition, by studying

Ngày tải lên: 05/11/2020, 01:32

... additional 3% are within 500 bp of a novel TSS (Figure S12) Interestingly, only ~72% of all CAGE sequencing reads map to TSS, indicating that the remaining 30% may originate from recapping events or ... cell lines The consequent reduction in the length of “intergenic regions” leads to a significant overlapping of neighboring gene regions and prompts a redefinition of a gene resulting in a tendency ... those of protein coding genes This is, for example, seen in the expression of the protein coding gene actin, gamma 1 (ACTG1), and the non-coding gene, H19 (Figure 3) ACTG1 transcripts are part of

Ngày tải lên: 20/10/2022, 02:42

... comparing the amounts of Mcm in total cellular proteins andchromatin-boundproteins, approximately one-thirdof total Mcm protein was found to be recoveredin the chromatin-boundfraction in HeLa cells ... Trang 2Mcm3 and4 in cancer cells than in normal proliferating cellsof human uterine cervix anddysplastic cells The results suggest that enhancedexpression of Mcm proteins plays a role in the malignant ... observedin dysplastic cells, suggest-ing that Mcm proteins are a goodindicator of proliferative or cancer cells in malignant tissues [28] Elucidation of how the expression of Mcm protein changes

Ngày tải lên: 20/02/2014, 23:20

... sedimenting in the first third of the gradient and attributable to short chains originating from newly initiated replicons In the course of further 25 min of reoxygenated growth, the incorporation of ... and of replicating DNA Cells grown on coverslips were labeled by adding 15 lM 5¢-bromodeoxyuridine (BrdU) 15 min before the end of the respective incubation conditions, in case of hypoxic labeling ... 1Analyzing changes of chromatin-bound replication proteins occurringin response to and after release from a hypoxic block of replicon initiation in T24 cells Maria van Betteraey-Nikoleit, Karl-Heinz

Ngày tải lên: 21/02/2014, 00:20

... binding of ligands to the D2 dopamine receptor may exhibit co-operativity, which can be accounted for in terms of interactions between binding sites in an oligomer There is also the question of ... pluronic F-68 The cells were maintained at a density of 0.5–2.5· 106 cellsÆmL)1and passaged every 2–3 days For infections, cells were seeded at a density of 0.3–0.6· 106 cellsÆmL)1 and infected when ... (HEK293 cells) A 2-h incubation was performed at room temperature on a rotating wheel before washing the cells twice with incubation buffer and resuspending the final pellet in 50 lL of incubation

Ngày tải lên: 21/02/2014, 00:20

... ldlD cells correlated with the defects in enzyme activity Interestingly, stable cells expressing mutants showing mild defects in enzyme activity behaved in a manner similar to the original CHO cells ... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated ... also observed in the cytoplasm, as seen for the wild-type protein Similar staining patterns were observed in other human cell lines, including hepatoma Hep3B cells and fibroblast 2fTGH cells (data

Ngày tải lên: 07/03/2014, 00:20

... decrease in intracellular cinnamate levels, suggesting feedback inhi-bition (i.e autoregulation) of PAL at a certain level of endogenous cinnamate [16] PAL in higher plants is coded by a family of ... The rarity of PAL in bacteria may be explained by the infrequency of phenylpropanoids in these species The bacterial PALs seem to be involved in biosynthe-sis of the antibiotics enterocin by S maritimus ... biosynthesis of the photoactive yellow protein chromophore of these bacteria The two recently discovered aminomutases, the phe-nylalanine 2,3-aminomutase (PAM) involved in taxol biosynthesis in Taxus chinensis

Ngày tải lên: 07/03/2014, 12:20

... because of the lack of detailed information about the functional contribution of individual components of the translational machinery Only some of the factors involved in the initiation of translation ... using cap-FLuc reporter mRNA in the absence or presence of increasing amounts of the indicated proteins (B) Functionality of the Ubx-IRES in a dicistronic reporter mRNA in vitro The effect of ... Auto´noma de Madrid, Spain Eukaryotic initiation factor (eIF) 4B is part of the protein complex involved in the recognition and binding of mRNA to the ribosome Drosophila eIF4B is a single-copy gene

Ngày tải lên: 07/03/2014, 15:20

... be induced by incubating cancer cells with oligomycin, an inhibitor of mitochondrial ATP synthetase [159,160] Similarly, reduction of OXPHOS by targeted disruption of frataxin, a protein involved ... protein (also named iPFK-2) is expressed in high levels in human tumors in situ Induction of this isoform is mediated by HIF-1, cMyc, Ras, src and loss of func-tion of p53 [73] Rapidly proliferating ... capable of reducing the level of fructose 2,6-P2 inde-pendent of the phosphorylation state of iPFK-2 Reducing the level of fructose 2,6-P2 and thus the activity of PFK-1 improves the supply of glucose

Ngày tải lên: 14/03/2014, 23:20

... ATP-binding domain of HSP70 is critical for sequester-ing AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain of HSP70 was indispensable for inhibition of ... with respect to the role of HSP70 in the inhi-bition of the release of Smac and apoptosis The role of the ATP-binding domain of HSP70 in the prevention of the release of Smac and apoptosis after ... eliminating inhibition of caspases by inhibitor of apoptosis protein (IAP) and is known to be a new and important regulator of apoptosis in a variety of cancer cells The evidence obtained in our

Ngày tải lên: 16/03/2014, 01:20

... comparison of the amounts of GST fusion proteins used in each case Some degradation of the GST fusion proteins is evident from the presence of products running at the position of GST itself (E) Binding ... Previous work has shown that mutation of the phenylalanine to alanine in the TOS motif eliminates the binding of raptor to the C-terminal fragment of human 4E-BP1 in the overlay assay [19] (see also ... C-terminus of 4E-BP1 that are involved in its binding to raptor The data for the other truncation mutants shown in Fig 1C indicate that other regions of 4E-BP1 also contribute to stable binding

Ngày tải lên: 16/03/2014, 06:20

... domain As in thebinding studies with plant lectins, we routinely per-formed experiments to assess concentration depend-ence and inhibition of galectin binding by haptenicsugar fam-Profiling of ... imply thatthe a5b1-integrin is a binding partner of anoikis-indu-cing galectin-1 Trang 12Fig 5 Enhanced galectin-1 expression in p16INK4a-reconstituted cells Aliquots of total protein (200 lg) from ... chemiluminescence (top panel) Mass-spectrometric fingerprinting after digestion of the protein of this spot by trypsin ascertained identity to galectin-1, and quantification of the staining intensity

Ngày tải lên: 16/03/2014, 10:20