consistency stability and error analysis of numerical schemes

... identification of genuine 14-3-3 ligands Detailed analysis of the 14-3-3-asociated proteins found showed that 46 of them were exclusively present in one of the conditions ana-lyzed and 15 of them were ... percentage of breast tumors in the early stage of the disease, contributing to the transformation of cells and also to the further progression of breast cancer [42] On the other hand, downregulation of ... caspases andalso activation of the initiator caspase-8 [61], effectsthat are completely abolished by Bcl-2 and Bcl-xL[62,63] Fig 3 Analysis of expression levels of several 14-3-3 isoforms in

Ngày tải lên: 06/03/2014, 22:21

... BGP require individual diagnosis and therapy, and an understand-ing of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment ... Taiwan 3 Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan 4 Department of Pediatric, Division of Immunology and Allergy, University of Florida, Gainesville, ... of number 4 and number 5 peptide sequences (Fig 2B), and an antisense primer, designed on the basis of the amino acids of the number 2 pep-tide sequence (Fig 2B), were used in the first step of

Ngày tải lên: 07/03/2014, 12:20

... kinetic parameters of Y10F could not be measured at pH 9.0 due to enzyme instability. At pH 8.0 Y10F showed a k cat of 0.55 and K m of 47.2 (compared to a k cat of 3.5 and a K m of 24.9 in the wildtype ... pH 8.0, 8.3 and 9.0 bicine/HCl buffer and pH 9.0, 9.5 and 10.0 ethanolamine/ HCl buffer [36]. Structural analysis and electrostatics calculations Studies of chitinase structures and molecular ... moves into the ÔupÕ position and interacts with the substrate and Glu144. This rotation of Asp142 is accompanied by removal of a water molecule and adjustments of Tyr10 and Ser93 that seem to compensate

Ngày tải lên: 07/03/2014, 14:20

... 1Context-dependent effects of proline residues on the stabilityand folding pathway of ubiquitin Maria D Crespo, Geoffrey W Platt, Roger Bofill and Mark S Searle School of Chemistry, Centre for ... for folding and unfolding of WT*, kfoldand kunfoldare the folding and unfolding rates of the mutants derived from the chevron plot analysis, and bfis a constant describing the degree of native-like ... major fast folding phase of wild-type yeast ubiquitin (WT*) and of a number of nondisruptive single-point Ala mutants and several double mutants, using F-value analysis and Brụnsted plots, shows

Ngày tải lên: 07/03/2014, 16:20

... Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p and Pex6p involves ... function of Pex1p and Pex6p A B Fig 2 Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites. A schematic representation of the ... parts of the N-terminal fragment and⁄ or the second cassette; D C Fig 5 Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex6p on the morphological appearance of peroxisomes

Ngày tải lên: 07/03/2014, 16:20

... the mode of C5a peptidase maturation and determination ofthe exact borders ofits pro-sequence region We have also evaluated the thermal stability and kinetic parameters ofC5a peptidase and the ... this peptide and then plotted as a function of time The identity ofpeptides was determined by NH2-terminal sequence- and mass spectral analysis Treatment ofthe QTPDEAAEETI and AEETIADD ANDL synthetic ... transition with Tmof50 and 70
C suggesting melting of different parts ofthe molecule with different stability Unfolding of the less stable structures was accompanied by the loss ofproteolytic activity

Ngày tải lên: 08/03/2014, 16:20

... Vicinity of aThr109 of the Co-type NHase The wild-type enzym e, aT109S mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of ... the formand size of the hydrophobic pocket Fig 5 Superimpositioning of the metal centers of the wild-type enzyme and mutants of the cobalt-containing nitrile hydratase (Co-type NHase) and the ... mutational and structural analysis of the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile,

Ngày tải lên: 16/03/2014, 16:20

... regulating IL-18 expression and process-ing in this species, the first report of such a phenomenon for this cytokine Materials and methods Cloning and sequencing of genomic DNA and cDNA All products ... with an equal volume of phenol/chloroform (24 : 1, v/v) and once with chloroform Genomic DNA was precipitated with two volumes of cold ethanol, washed with 70% (v/v) ethanol, and dissolved in TE ... the human IL-18 gene, consisting of six exons and five introns The Fig 2 Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the predicted Fugu/tetraodon

Ngày tải lên: 16/03/2014, 16:20

... (K 2 0.667 [30]). A value of 1.36 was taken for the refractive index n [31]. MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after tryptic digestion of the labeled and nonlabeled Ag-NPA-1 ... chromophore of ABA-1, the single Trp of Ag-NPA-1 is a sensitive marker of the protein conformation and its emission reflects conformational changes after ligand binding. Analysis of FRET from the ... incorporation of the ligand. Binding of DAUDA to Ag-NPA-1 caused  20% quenching of the emission of Trp17 indicating FRET between the single Trp residue and the dansyl group. We calculated a value of 3.74

Ngày tải lên: 16/03/2014, 18:20

... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein. Results and Discussion Annotation of CMs in M. tuberculosis ... addresses *Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea Division of Molecular Biology, Department of Zoology, Sri Venkateswara ... alignment that helix 3 of 90-MtCM is a shorter version of helix 3 of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig. 4C) corre- sponding to Ser84 and Gln88 of EcCM as discussed below. During

Ngày tải lên: 17/03/2014, 17:20

... degradation of the poly(A) tails of single-stranded mRNAs in a highly processive mode. PARN participates in diverse and important intracellular processes by act- ing as a regulator of mRNA stability and ... bind with the 3¢-poly(A) tail and the 5¢-cap of the mRNA, and may be important to the allosteric regulation of PARN [19–23]. In general, the structure and stability of the domains, as well as their ... stimulates rate of poly(A) removal and amplifies processivity of degradation J Biol Chem 276, 27923–27929 Copeland PR & Wormington M (2001) The mechanism and regulation of. .. domain of poly(A)-specific

Ngày tải lên: 23/03/2014, 04:21

... respectively Error bars denote the standard error of the mean for three independent measurements. Trang 3epidermis and intestine of 6-day-old larvae and adultheads and ovaries, was blotted and hybridized ... organization of this gene and analyzed its structural evolution Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp ... all insect tissues and developmental stages Sequence analysis of the extended region of the longer transcript revealed the existence of three mRNA instability motifs (AUUUA) and five poly(U) tracts,

Ngày tải lên: 23/03/2014, 06:20

... G or C. Analysis of DNA binding by hydroxyl radical footprinting and interference assays A more detailed picture of the binding of HAKN1 to its target site was obtained by the analysis of footprint- ing ... incorporation of Ile50 and Arg55 must have been particularly important. Other homeodomains that contain Ile50 and Arg55 are those of the TGIF, Meis and Bell families [22] and yeast MATa1 [36]. TGIF and ... expression and purification of recombinant proteins HAKN1 homeodomain and C-terminal sequences were amplified and cloned in-frame into the EcoRI and PstI sites of the expression vector pMAL-c2 (New England

Ngày tải lên: 23/03/2014, 13:20

... Ellis‟s (1994) procedural analysis of errors; collection of sample of learner language, identification of errors, description of errors, explanation of errors, and evaluation of errors in analyzing ... essays and collected the errors for the study 2 Identification of errors: Actual errors 3 Description of errors: Categorization of errors based on their specific nature 4 Evaluation of errors: ... occurrences of two types of errors; Interlanguage errors and mother tongue (MT) interference errors have been compared and the results show that the percentage of the occurrences of Interlanguage errors

Ngày tải lên: 24/03/2014, 19:20

... financial analysis is usually referred as the viability, stability and profitability of a business and business analysis usually... Business and Financial Analysis of BP ... analysis of ratios breakeven analysis and various trends 24 Business and Financial Analysis of BP with comparison to Shell PLC 2011 The overall financial ratio analysis ... and the Shell PLC are... rudiments and icons of the business and financial analysis, there effects and significance in the pertinent field 17 Business and Financial Analysis

Ngày tải lên: 25/03/2014, 11:06

... separately by Walhout and coworkers [31], Mrowka and colleagues [19], and Aloy and Russell [32] have reported on this type of artifact and have discussed a relationship between it and some bait proteins' ... to understand better the experimental errors Each VBP protein p has np unreciprocated edges, and under the assumption of randomness we expect the number of unreciprocated in-edges and out-edges ... number of proteins, then n + m = ily of solutions (a one-dimensional set of solutions in a space of three variables; Figure 6) To gain insight into the prevalence of FP and FN stochastic errors,...

Ngày tải lên: 14/08/2014, 08:20

... result list of load cases, superposition files and influence lines 3.64 Version 8.38.03 • • • • Use of combination codes “SupOrX” and “SupAndX” for superposition files corrected New command “PLSCFAC” ... shrinkage calculation is numerically extended to cover more exactly “creep of creep” effects ( it is possible to decrease number of time steps ) Extensions of British Standard live load calculation ... to choose between E- and Fformat of the written values Information about units added to the plot file of the action PlInfl, screen plot Results/ PlInfl and to the list file of the action DoList/Influence...

Ngày tải lên: 06/09/2012, 15:18

... Disclaimer and Copyright Disclaimer Much time and effort have gone into the development and documentation of RM2000 and GP2000 The programs have been thoroughly tested and used The user accepts and ... the two load sets and the two loading cases (e.g.: L.C is a UDL of 10kN/m from Elem to Elem 20 and L.C is a Settlement of 0.001m at the beginning of element 1200.) Choose AddCon and insert the ‘Additional ... the program and the documentation remain with TDV Austria Use of the program and the documentation is restricted to the licensed users Unlicensed use of the program or reproduction of the documentation...

Ngày tải lên: 06/09/2012, 15:55

... Definition of loading and construction stages "RECALC Definition of calculation parameters and start of the calculation "RESULTS Viewing of results and creating of output files (plots and listings) ... from the icons at the top of the RM2000 screen), write the sequence of commands and save it as ‘filename.tcl’ The summary and the syntax of the commands to be specified and used in the TCL script ... displayed on the right hand side of the screen #NODE Definition of nodes and their attributes #ELEMENT Definition of elements and their attributes #TENDON Definition of tendons and their attributes...

Ngày tải lên: 06/09/2012, 15:55

... conditional breakfast They often have cereal or porridge, egg and bacon followed by toast and marmalade and coffee and tea or they may just have a toast and marmalade and coffee and tea *Elevenses - ... Definition of word meal - Field of word meal in English and in Vietnamese equivalents - Cultural and linguistic analysis of the English word meal Chapter III Cultural and linguistic analysis of Vietnamese ... People often drink a glass of mild and a sandwich 3.1.6 Parts of meal Basically, English meals and meal time are quite clear Even name of courses are also: starter, main course/ meat course and...

Ngày tải lên: 15/04/2013, 15:11