classifi cation of theories

... for the rapid detection of hydrophobins from a large number of small samples, we have investigated the potential of intact-cell MALDI-TOF MS (ICMS) Initial applications of ICMS on fila-mentous ... Intact-cell MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H jecorina grown on malt agar The masses displayed have an error of about 0.1%, so peaks of 7232 ... Trang 4lation of mass data is achieved by the calculation ofsuccessive deletions of terminal amino acid residues of all six hydrophobins providing this single match A rapid means of identifying

Ngày tải lên: 19/02/2014, 02:20

... Trang 1Identification of different isoforms of eEF1A in the nuclear fractionof human T-lymphoblastic cancer cell line specifically binding to ... the mechanism of action of our cytotoxic GT oligonucleotides In this article, we report the identification of eukaryotic elongation factor 1 alpha (eEF1A) as a nuclear component of the CRC in ... presence of eEF1A was confirmed by Western blot analysis using the specific antibody, anti-eEF1A.Only the higher magnification of IEF of the eEF1A region is reported. Trang 9cells, the presence of two

Ngày tải lên: 21/02/2014, 00:20

... Amplification of cDNA templates with a set of primers corresponding to the Xenopus EF1a elongation factor cDNA (280 bp) as internal control of the poly(A) + RNA (C) Tissue comparison of the level of ... desensitization of the responses (Lower) Responses of the three xTRHR subtypes to different concentrations of TRH The maximum current amplitude of each recording was meas-ured and reported as a function of ... xTRHR subtypes. Fig 6 RT-PCR distribution of Xenopus TRHR in various tissues (A) Amplification of the middle portion of xTRHR cDNA (550 bp) using the two sets of degenerated oligonucleotide primers,

Ngày tải lên: 21/02/2014, 03:20

... their code base? (2) What is the degree of automation of the detection aspect of the approach? (3) What is the degree of automation of the prevention aspect of the approach? (4) What in-frastructure ... present an extensive review of the different types of SQL injection attacks known to date For each type of attack, we provide descriptions and examples of how attacks of that type could be performed ... practitioners are often unaware of the myriad of dif-ferent techniques that can be used to perform SQLIAs Therefore, most of the solutions proposed detect or prevent only a subset of the possible

Ngày tải lên: 05/03/2014, 23:20

... the expression of MT2A was upregulated in Tca8113⁄ PYM cells MT2A, as the main isoform of MTs, may closely mediate the resistance of Tca8113⁄ PYM cells to PYM BMP2 is a member of the transforming ... because of the methylation of the promoter, and restoration of DKK1 expression can induce apoptosis and suppress colony formation [42] As a suppressor of cancer, the downreg-ulated expression of DKK1 ... b-Isoform of regulatory subunit B55, protein phosphatase 2 isoform a growth factor receptor substrate 2) tract-binding protein associated) 4-hydroxylase), a-polypeptide I Trang 5Confirmation of

Ngày tải lên: 06/03/2014, 22:21

... examined the propensity of the different isoforms of IGFBP-1 to backbone cleavage in the presence of this partially purified specific protease Results Purification of IGFBP-1 isoforms The amniotic ... 280 nm of the pooled fractions (assuming an absor-bance value of 1.42 for a 1 mgÆmL)1 solution of IGFBP-1 as calculated on the basis of the amino acid Fig 1 Purification of IGFBP-1 isoforms The ... degradation of IGFBP-1 isoforms. The y-axis shows the percentage of the ratio between the spot area of the degraded and that of the untreated protein measured using IMAGE J 1.37V software. Trang

Ngày tải lên: 07/03/2014, 00:20

... and Discussion Site-directed mutagenesis of the target arginine of dinitrogenase reductase An indication for the covalent modification of one subunit of dinitrogenase reductase in Azoarcus sp ... localize a labile modification within a large peptide of 31 amino acid res-idues The present study provides a method suitable for the identification of so far unknown protein modifications on nitrogenases ... other types of post-transla-tional modifications on nitrogenase that do not neces-sarily lead to the inactivation of the enzyme Gallon et al.[23] proposed a palmitoylation of both dimers of nitrogenase

Ngày tải lên: 07/03/2014, 02:20

... Russian Academy of Sciences, St Petersburg, Russia 4 Department of Molecular Basis of Medicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia A variety of human diseases ... Summary of purification scheme (B) Pre-parative EMSA elution profile (C) Testing of the rpL32 fragment ( )24 +11)-binding activity of 21st to 27th fractions obtained on a prepara-tive EMSA purification ... fragment ()24 +11). Trang 6Fig 3 Identification of p56 polypeptide as Kru¨ppel-like transcription factor ZF5 by MALDI-TOF assay (A) Fragment of MALDI-TOF spectrum of p56-derived peptides (B) Mascot

Ngày tải lên: 07/03/2014, 05:20

... and downregulation of Bcl-2 Inhibition of caspase-8 attenuated Bax and Bak activation, but did not affect phosphorylation and downregulation of Bcl-2 Expression of a mutant form of Bcl-2 (S70A-Bcl-2) ... transmembrane potential of these cells was determined by assaying the retention of DioC 6 (3) added during the last 30 min of treatment After removal of the medium, the amount of retained DioC 6 (3) ... trans-membrane potential of these cells was determined by assaying the retention of DioC6(3) added during the last 30 min of treatment After removal of the medium, the amount of retained DioC6(3)

Ngày tải lên: 07/03/2014, 05:20

... samples, a melting peak area ratio of 2 : 1 is indicative of gene duplication [26] An example of gene quantification using hybridization probes is the detection of the auto-somal dominant demyelinating ... data revealed that the two CYP17 isoforms were not the product of two alleles of the same gene, but two separate CYP17 genes encoding the two unique CYP17 isoforms This novel finding was subsequently ... steroid pathway Although the hydroxylase activities of these isoforms are similar, the lyase activity of CYP17 ACS+ results in the produc-tion of significantly more glucocorticoid precursors, essential

Ngày tải lên: 07/03/2014, 06:20

... Coomassie staining of an SDS⁄ PAGE gel revealed that fractions corresponding to peak I A Fig 1 Separation and identification of PKA C-subunit isoforms from mussel PAM (A) Elution profile of C-subunit ... sample of C-subunit purified from bovine heart (fraction CB), consisting mainly of the A B C Fig 2 Separation and identification of PKA C-subunit isoforms from mussel mantle tissue (A) Elution profile ... purpose of the work described in this article was to investigate the possible existence of different isoforms of the PKA C-subunit in the sea mussel M galloprovincialis Results Separation of different

Ngày tải lên: 07/03/2014, 06:20

... with a mass difference of 6 or multiples of 6 As a quarter of the reference Fig 4 Identification of emerin and of tyro-sine-phosphorylation sites (A) MS ⁄ MS spectrum of the mouse-emerin peptide ... perper-vanadate treatment of HeLa cells (20 large dishes) grown in the presence of the heavy amino acids 13C6-arginine and 13C6-lysine created a state of hyperphosphoryla-tion of all cellular proteins ... mostly on the transient phosphorylation of either serine⁄ threon-ine or tyrosthreon-ine residues [13,14] The identification of such post-translational modifications is efficiently addressed by specialized

Ngày tải lên: 07/03/2014, 12:20

... modification (de-glyco-sylation) Similarly, a reversal of the changes caused by injury could be a result of the survival and out-growth of IB4-positive fibers, of an increased expres-sion of the ... above Lanes 8–10 of the gel were stained with Coomassie Brilliant Blue R250 Lane 1, marker; lane 2, 15 lL of supernatant of the extracted light membranes; lane 3, 15 lg of pro-tein of the light ... of nociceptive C-fibers (see the Introduction and the references therein) The aim of our study was to elucidate the molecular identity of the glycoconjugate carrying the Fig 5 Identification of

Ngày tải lên: 07/03/2014, 16:20

... trade-off, in terms of effects of measurement uncertainty on the one hand and the effects of nonlinearities on the other hand, when choosing the size of parameter perturba-tions Impact of sampling ... identi-fication of a network consisting of five genes The net-work is a modification of the four gene netnet-work used in the previous example, obtained by adding a fifth Table 1 Comparison of estimation ... combination of compo-nents [10] A drawback of this method, for the appli-cation to biological systems, is the fact that the perturbations are assumed to be instant changes of the concentrations of intermediates

Ngày tải lên: 07/03/2014, 17:20

... globotetraose-containing Hep3-glycoform (HexNAc1Hex4) of NTHi isolate 1209 Structure of the core region of the Hep4-glycoforms In the 1H NMR spectrum of OS-1¢-B (Fig 5C), anomeric resonances of the three heptose ... data indicated the presence of two subpopulations of glycoforms; a major subpopulation in which the glycoform compositions comprised three heptoses and PCho (Hep3-glycoforms), and a minor subpopulation ... corresponding to selected Hep3-glycoforms are labelled (B) Ions corresponding to selected Hep4-glycoforms are labelled. Table 3 Structures of the Hep3-glycoforms of NTHi isolates 1209 and 1233 as

Ngày tải lên: 08/03/2014, 08:20

... independent quantification of their activity in vivo requires the use of isotopic tracers [5,35,38] The extent to which the use of MS measurements of biomass hydrolysates after the introduction of the 13C ... form of the stoichio-metric matrix enabled the identification of the net fluxes in each group (i.e the nonzero elements of the two vectors in the null space [53] of the reduced row-echelon form of ... concerning the application of stable isotope techniques It includes an involved set of anaplerotic reactions and two parallel pathways in the lysine biosynthesis route Both of these groups of reactions

Ngày tải lên: 08/03/2014, 08:20

... Kessler Department of Clinical Medicine, University of Oxford, UK Introduction The genus Yersinia consists of three pathogenic species that are agents of a variety of diseases, one of which was historically ... GTPase-binding capacity of YpkA and OTUB1-mediated increase in susceptibility to infection (Fig. 6). The binding of small GTPases has been reported to be independent of the kinase activity of YpkA [16]. ... can be achieved either by induction of RhoA mRNA expression, deubiquitination of RhoA itself or promoting activation of RhoA, possibly through the manipulation of RhoA-specific guanine nucleotide...

Ngày tải lên: 16/02/2014, 15:20

... percentage of amino acid coverage in the matched proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new ... maximum peptide mass tolerance of 150 p.p.m., one missing cleavage allowed, a fixed modifi- cation of cysteines by iodoacetamide (carbamidomethyl), a variable modi cation of methionines (oxidation) ... patterns of activity [13,23]. It has been suggested that the pres- ence of several well-separated peaks in the case of cell- ulosomes from C. cellulovorans may be partly due to the existence of various...

Ngày tải lên: 18/02/2014, 08:20

... mean of the fluorescence signal of at least eight wells containing aliquots of the same solution. The morphology of Ab aggregates formed from solutions incubated as above but in the absence of ThT ... two-step puri cation results in a highly pure product, and yields 10–20 mg of Ab(M1–40) per liter of culture. In the example shown in Fig. 3, 30 mg of peptide was obtained from 2.2 L of bacterial ... broader coverage of struc- tural assignments to the N-terminus of Ab. Moreover, the procedures described here are also suitable for expression and puri cation of mutant versions of Alzheimer’s...

Ngày tải lên: 18/02/2014, 13:20

... four molecules of 3OH-C12:0, two of C10:0, and one of C12:0 [10]. When compared to this struc- ture, the lipid A of O35ElpxX lacks two decanoic acyl (C10:0) substituents, and that of O35ElpxL lacks ... NaCl ⁄ chloroform (1 : 1 v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase was re-extracted with de-ionized water to remove traces of salts, and ... Hofmann K & Stoffel W (1993) TMbase – a database of membrane spanning protein segments. Biol Chem Hoppe-Seyler 374, 166 . 44 Claros MG & von Heijne G (1994) TopPred II: an improved software...

Ngày tải lên: 18/02/2014, 14:20