checklist for phenotypic characterization of chickens

... 9Figure.1 Phenotypic characterization of C coronarium and its different mutants Figure.2 Phenotypic characterization of C coronarium and its different mutants Trang 10Figure.3 Phenotypic characterization ... experiment was conducted to study the phenotypic characterization of mutants from different species of annual chrysanthemum by treating the seeds of different species of Chrysanthemum coronarium (Cco), ... cm Date of Planting – September Date of Transplanting – October (30 days old seedling) Experimental methods The experiment broadly consisted of two parts: 1 Phenotypic characterization of mutants

Ngày tải lên: 12/03/2020, 21:55

... 4.3 Graph of comparison of the exact methods for Type II instances 121 4.4 Graph of comparison of the exact methods for Type II instances 127 4.5 Graph of the heuristic methods for Type ... Comparison of the quality of exact methods for Type II instances 101 3.6 Results of the polynomial heuristic methods for Type I instances 101 3.7 Results of the Polynomial heuristic methods for ... of the exact methods for Type II instances 119 4.4 Comparison of the quality of exact methods for Type I instances 120 4.5 Comparison of the quality of exact methods for Type II instances

Ngày tải lên: 11/07/2021, 16:55

... reproducibility of histological assessments of the cores of nodal tissue among the three pathologists had a kappa score of 0.916 (95% CI: 0.756–1.07) Of the 50 samples tested for reproducibility, ... confirming the value of the PDUS-guided CNCB for detecting lymphoma Waiting time to biopsy The median waiting time for performance of interventionist procedure (from biopsy indication to perform itself) ... total cost of the biopsy program was much lower for the core-needle group than that for the standard group By using Italian values for direct costs of interventionist procedures, the cost for one

Ngày tải lên: 04/12/2022, 16:07

... initial reduction of a nonflavin redox center with the first reduction resulting in what appears to be an EH 2 form of the enzyme. Addition of a second of NADPH results in the formation of an apparent ... 2e – reduced form of the enzyme is referred to as the EH 2 species [17]. Addition of a second equivalent of reduced nucleotide substrate results in reduction of the enzyme-bound FAD, forming the ... nm peaks of the E and EH 2 forms, respect- ively. This result is inconsistent with reduction of the FAD and consistent with the formation of an EH 2 Æ NADPH complex, although further characterization

Ngày tải lên: 07/03/2014, 17:20

... Induction of meiotic oocyte maturation occurred after injection of OA into Xenopus [16] or starfish oocytes [17] This inhibitory activity of PP2A is shown to be due to the presence of a trimeric form of ... templates for DNA sequencing 5¢-RACE technique 5¢-RACE reactions were performed on about 1.5 lg of total RNA of prophase X laevis oocytes with 1 U of AMV-reverse transcriptase (5¢/3¢RACE kit, Roche) For ... reaction was performed using primers containing the translation stop of the different isoforms to obtain specific cDNA After purification of this cDNA, semiquan-titative PCR was performed PCR products

Ngày tải lên: 08/03/2014, 08:20

... characterized consist of monomeric proteins of
45–55 kDa except for the Pseudomonas sp 1158 enzyme which has a molecular mass of 100 kDa and the Amycolatopsis enzyme which has a molecular mass of 36 kDa ... positive for catalase, oxidase and nitrate reduction but negative of hydrolysis for gelatin and starch Therefore, according to all above analysis results, the strain Iso1 was clearly a strain of V ... Spring Harbor, New York. 40 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem

Ngày tải lên: 08/03/2014, 16:20

... log of the observed first-order rate kinetics of inactivation constant against log of EDTA concentration n, the slope of the plot, was calculated as 0.44 and estimates the number of molecules of ... Cristofoletti et al Aphid midgut aminopeptidase The thermodynamic parameters of activation for A pisum APN (Fig 1D) were calculated by Arrhenius plot (plot of kcat against ⁄ T) From the slope of ... was a slight preference for tripeptides (Table 1), as judged by a comparison of kcat ⁄ Km values for peptides of the Leu-(Gly)n series which differ only in the number of Gly residues (Table 1)

Ngày tải lên: 16/03/2014, 12:20

... overlapping fragments of GAS1 were amplified using two sets of primers For sGas1E161Q-H, a pairing of the forward (5¢-TGTTAGTAACTTGATTACCGGCGAAG-3¢), and a pairing of the forward primer FMGLN161 ... of an internal b(1,3)-glycosidic bond of a donor laminarioligo-saccharide followed by the transfer of the new reducing end to the nonreducing end of an acceptor molecule, with the formation of ... protonation state of the carboxyl group of their side-chain [7] The aim of the present study was to express Gas1p at high levels for biochemical and structural characterization of the protein as

Ngày tải lên: 16/03/2014, 18:20

... allowing formation of a large number of conformational antigen- binding surfaces including planar and ridged topologies [2]. The orientation of the loops is maintained by a combination of their ... responsible for immuno- surveillance, there is strong evidence for their functional role in antigen binding. First, an analysis of mutational patterns of membrane-bound and secreted forms of nurse sharkIgNARsindicatedthattheyaremutatedinthelatter form, ... decrease in activity of the immobilized Tom70. Regeneration of the Tom70 surface was achieved by running the dissociation reaction to completion before the next injection of analyte. For binding experiments

Ngày tải lên: 17/03/2014, 10:20

... expressed-sequence tag; gACE, germinal isoform of angiotensin-converting enzyme; tACE, testicular isoform of angiotensin-converting enzyme. Trang 2somatic isoform exhibits two catalytic domains and ... different ACE isoforms have been identified [15] and it will prove very informative to determine whether or not every isoform displays a transmembrane region Knowing whether all isoforms in Astacus ... questions about both the evolution of ACE in different groups of animals and the physiologi-cal significance of membrane bound isoform compared to the soluble form, which was almost exclusively

Ngày tải lên: 30/03/2014, 01:20

... unpaired data mode of the algorithm As a cut-off for significance, an estimated FDR of 2.6% was chosen by the tuning parameter delta of the software The significance level of each gene was given ... and a cut-off for fold-change of differential expression of was used Hierarchical clustering analysis (HCA) was used to determine components of variation in the data in this study For these analyses ... genes of a sample, queried their expression values and made the decision of "part of the class" or "outside the class" For each classifier and LOOCV iteration, the frequency of a gene (how often

Ngày tải lên: 18/06/2014, 15:20

... occurred [1] Therefore, the development of tools for the detection of viruses, the characterization of their genomes and the study of their evolution, becomes important, not only for basic scientific ... provided The use of the CODEHOP methodology for the analysis of larger regions of viral genomes is presented along with the general application of this technology for the identification of viral species ... sequence information for phylogenetic characterization, gene structure determination and genome analysis is reviewed While this review describes techniques for the identification of members of the

Ngày tải lên: 18/06/2014, 22:20

... 1 of 12 (page number not for citation purposes) Virology Journal Open Access Research Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of ... the family of ion peaks, gave a protein with molecular mass of 13,794 ± 4 Da (Figure 1). The lack of recovery or detection of proteins of the size of CP subunits is likely the result of retention ... molecular mass of 13,790.71 kDa; peptide B of 13,790.71 kDa; peptide C of 13,796.70 kDa; peptide D of 13,793.60 kDa; peptide E of 13,795.70 kDa. Underlined sequences represent the active site of the

Ngày tải lên: 19/06/2014, 08:20

... consisted of 30 cycles of 94°C for 1 min, 58°C for 1 min. and 72°C for 2 mins. JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for 1 min, 45°C for 1 min. and 72°C for 2 mins. ... conditions consisted of 30 cycles of 94°C for 1 min., 58°C for 1 min. and 72°C for 2 min. MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for 1 min, 59°C for 1 min and 72°C for 2 mins. ... &C2R: PCR conditions consisted of 94°C for 10 mins followed by 35 cycles of 94°C for 30 secs., 60°C for 1 min and 72°C for 1.5 mins followed and extension of 7 mins at 72°C. AC1F &AC1R

Ngày tải lên: 19/06/2014, 08:20

... Trang 1Appendix C Page 1 of 31 Performed by the Office of Inspector General This appendix includes guidance for reviewing the Office of Inspector General’s (OIG’s) audit of the agency’s principal ... used for the OIG’s monitoring of the work of an independent public accountant (IPA) where the IPA signed the report as the principal auditor This guidance is provided in Appendix F - Checklist for ... Page 8 of 31 and how assets of the entity could be misappropriated? • An emphasis on the importance of maintaining professional skepticism throughout the audit regarding the potential for material

Ngày tải lên: 19/06/2014, 15:20

... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterization of ... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing...

Ngày tải lên: 18/02/2014, 16:20

... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... materials, and that mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules ... strain Δε0 for a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete,...

Ngày tải lên: 22/02/2014, 08:20

... comprehensive list of variables for describing the phenotypic and genetic characteristics of cattle, sheep, goats and chickens as the basis for systematic phenotypic characterization of these species ... part of phenotypic characterization Box How to complement genetic characterization with phenotypic characterization – an example An integrated phenotypic and genetic characterization study of three ... case is one of the roles of phenotypic characterization and is a prerequisite for effective assessment of AnGR diversity and determining whether or not it is being eroded Phenotypic characterization...

Ngày tải lên: 31/03/2014, 22:20

... proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the protein of interest As numerous amine functional ... conjugate formation Bioconjugation of proteins to carboxylated QDs have been performed with the use of EDC alone [7] Despite the simplicity of a one-step reaction, the drawback involves a degree of ... net-charge of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including the polydispersity Page of 15 (page number not for citation purposes) Journal of...

Ngày tải lên: 11/08/2014, 00:22

... of antiCCP-antibodies or RF) and genetic (possession of SE alleles) parameters predictive of more severe RA Unfortunately, information on the degree of radiographic joint damage at the time of ... achieved with the IMPACT platform, we performed 21 replicate measurements of each of nine markers within one run on the IMPACT platform The intra-assay coefficients of variance (CV) ranged from ... coefficients of 0.92 for RF and 0.97 for CRP (Figures 2b and 2c) Analysis of the bone-turnover markers with IMPACT was previously described, the results of which correlated well with those of corresponding...

Ngày tải lên: 12/08/2014, 17:22