cell lines and their use in published research

... 1PROBIOTICS AND THEIR USE IN AQUACULTURE April 2006 10th Aquaculture Insurance and Risk Management Conference Presented by: William P Long Chief Executive Officer Epicore BioNetworks Inc. Trang ... Nitrite: very toxic to crustaceans and fish  And is food for disease causing Vibrio  Nitrate: too much causes algae crashes  Pond bottom waste: release nutrients in older ponds to promote natural ... efficient to use cost- Cheaper to use probiotics than antibiotics Trang 12PROBIOTICS AND VIRUSES Anecdotal evidence that probiotics mitigate pathogenicity of WSSV virus – Pond in Vietnam saved

Ngày tải lên: 22/09/2017, 11:14

... than in SAP patients showed the differential degrees of CD4+ T cell mediated cellular immunity dysfunction in AMI and SAP patients CD8+ T cells kill virus-infected cells and tumor cells and play ... phycoerythrin-cyanin 5.1 (PC5) Cells were identified by combinations as follows: CD3 (FITC)/CD16 (PE)/CD56 (PC5) (NK cells), CD3 (FITC)/CD4 (PE)/CD8 (PC5) (CD4andCD8 cells), and CD19 (PE) (B cells) In ... activating receptors and play an important role in targeting NK cell responses toward abnormal cells and eventually the cell lysis [24, 25, 26, 27] In our current study, gene expressions of activating

Ngày tải lên: 15/01/2020, 23:38

... between PPARβ/δ and RAR receptors is regulated by the levels of two key intracellular ligand-binding proteins: fatty acid-binding protein 5 (FABP5) and cellular retinoic acid-binding protein 2 (CRABP2) ... tar-geting PLZF-binding sites, leading to cellular retinoic acid-binding protein 1 (CRABP1) upregulation The CRABP1 protein is structurally similar to the cellular retinol-binding proteins, sequesters ... rapidly dividing embryonic cells and tumors with epithe-lial and mesenchymal origins [95] HMGA2 was also de-tected in some retinoid-resistant NBL cell lines In NBL cell lines, a causal link between

Ngày tải lên: 03/07/2020, 00:52

... Antioxidant Vitamins and Their Use in Preventing Cardiovascular Disease Dan Farbstein *, Adena Kozak-Blickstein and Andrew P Levy Technion Faculty of Medicine, Technion Israel Institute of Technology, ... in endothelial cells Looking at intracellular signaling, L-ascorbate was shown to decrease p53 levels and increase phosphorylation of the cell cycle regulator Rb, thus rendering it inactive and ... R.; Lin, T.J Protein phosphatase 2A and protein kinase Calpha are physically associated and are involved in Pseudomonas aeruginosa-induced interleukin 6 production by mast cells J Biol Chem 2002,

Ngày tải lên: 01/11/2022, 08:31

... transporters, and receptors as well as GJs (Binder and Steinhauser, 2006; Steinhauser et al., 2012; Binder and Carson, 2013) In the following, the influence of AEDs on GJs and their potential ... immune cells (T and B cells), although the initiating cause is still unknown and several contributing factors such as genetic predisposition, infec-tions and vaccination, vitamin D deficiency, and ... multiple sclerosis, (3) glioma and antineoplastic and anti-inflammatory agents that are used in treating brain tumors, and (4) epilepsy and anticonvulsants that are widely used for seizures therapy

Ngày tải lên: 02/11/2022, 11:39

... glands namely epidermis, secretory glands in the dermis along with intrinsic components including drugs, medication traces, metabolites and extrinsic contaminants namely blood, food contaminants, ... diluted ten times (1 × 107 CFU/mL) and used as inoculum in testing for bactericidal activity 2.5 Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration ... Mueller-Hinton broth and 100 ml Erlenmeyer flask were inoculated and these were kept in a shaker at 200 rpm for 24 ± 2 h and again incubated at ~ 37 °C Further, an optical density of McFarland of

Ngày tải lên: 26/07/2023, 07:38

... depending on their origin [18] Methods Cell lines and cell culture conditions The selected cell lines included cells of primary and meta-static origin Thirteen human melanoma cell lines were used ... therefore resulting in intracellular accu-mulation of chemokine receptors and interacting che-mokines Interestingly, the only chemokine that was found to be secreted in two cell lines in our study, ... (discontinuous black line) are shown for CXCR4 in the WM-115 cell line (A) and for CCR10 in the WM-266.4 cell line (B) Corresponding immunocytochemical staining of CXCR4 in WM-115 (C) and CCR10 in

Ngày tải lên: 05/11/2020, 01:20

... of conveying what speakers mean linguistically, and it is most commonly used in spoken language Identifying the possible interpretations and discovering the implied meanings of the information, ... Said, Understanding Organization, Connecting Content and Making Inferences (ETS, 2007) One of the most challenging types of question in the listening section of TOEFL test is inference since test-takers ... of linking words (25.4%) and determiners (9.4%) in lectures is much higher than that in conversations (linking words: 16.9%; determiners: 5.6%) Additionally, adjectives account for 1.6% in

Ngày tải lên: 09/01/2020, 11:14

... yielding CMS lines with good combining ability is a pre-requisitefor hybrid development in cabbage Twenty three genotypes (15 CMS lines+ six maintainer lines + two checks namely KGMR-1 and Varun) ... (CMS) lines along with their maintainer lines (six) developed and maintained in the Department of CSKHPKV, Palampur along with two checks of cabbage (commercial hybrids –KGMR-1 from public and ... „Vegetable Research Farm‟ of the Department of Vegetable Science and Floriculture, CSKHPKV Palampur with the objectives to identify the promising CMS lines and their maintainers and to find out

Ngày tải lên: 02/03/2020, 11:36

... represent standard deviation. Figure 3 Affinity of cell surface-specific V H Hs to living C reinhardtii cells Live-cell ELISA analyses comparing binding affinities to C reinhardtii cells of various ... analyses comparing the binding affinity of V H H B11 to living Chlamydomonas reinhardtii (cc124) cells (blue line) and other living algae cells (Chlorella, red line; Nannochloropsis, purple line; Coccomyxa, ... binding of V H H B11 to Chlamydomonas and to other algal cells in pond water samples Colorimetric analyses comparing the binding affinity of V H H B11 to living C reinhardtii (cc124) cells and

Ngày tải lên: 27/05/2020, 01:17

... CAMA1 were maintained in EMEM containing 10% FBS MCF-7 was maintained in EMEM containing 10% FBS and 10ug/mL human insulin SKBr3 was maintained in McCoy’s containing 10% FBS The cell lines were ... MDA-MB361, and MDA-MB453 were maintained in DMEM contain-ing 10% FBS MDA-MB415 was maintained in DMEM containing 15% FBS, 10ug/mL human insulin, and 10ug/ mL glutathione (Sigma, St Louis, MO) BT474 and ... HCC1428, HCC1569, HCC1954, and EFM192A were maintained in RPMI 1640 containing 10% FBS T47D and BT483 were main-tained in RPMI 1640 containing 10% FBS and 10ug/mL human insulin (Mediatech, Manassas,

Ngày tải lên: 19/09/2020, 21:32

... applied were used in developing countries, 45% in Europe, 24% in the USA and the remaining in other countries (Abhilash and Singh, 2009) In developing countries, the share of agrochemical use was ... waters are also monitored In this chapter, drinking water sources and the situation of drinking water supply in the Delta is presented Water using practice for drinking and selected pesticide residues ... pesticides directly by breathing in the chemicals while spraying or indirectly by drinking contaminated water or consuming foods products such as vegetables and fishes containing pesticide residues

Ngày tải lên: 19/07/2021, 07:09

... herefrom, that: 11 In polar coordinates, the sine-wave is represented in intensity and phase by a vector o c, and in combining or dissolving sine-wa/aes, they are to be combined or dissolved by ... space, and of which the complex quantity in a plane is only a special case M B STKINIHTTZ: In introducing j , first as distinguishing index and then defining it as V — my object was to introduce ... end of line, that is line grounded, and Line of infinite length are evidently of little practical interest, but of importance is only the case of a line feeding into an inductive or non-inductive...

Ngày tải lên: 04/06/2014, 12:33

... indicators and their use in sustainable agriculture and rural development 19 Land quality indicators and their use in sustainable agriculture and rural development 19 The context of indicators in FAO ... issues and participatory approaches Land quality indicators and their use in sustainable agriculture and rural development 11 CONCEPTS, DEFINITIONS AND PRINCIPLES "Land", the "functions of land", ... examples of improvements in land quality (e.g., results of soil conservation and land -use planning case studies); Land quality indicators and their use in sustainable agriculture and rural development...

Ngày tải lên: 06/07/2014, 08:20

... families for genotyping, selecting recombination events in recurrent backcrosses, using advanced intercross lines (AIL) or performing linkage disequilibrium and haplotype-based studies in outbread populations ... differences in the techniques used in different laboratories are minor ones, principally concerning the use or not of an automatic sequencing machine for size determination For SNP genotyping, this ... just using SNPs in animal genetics 277 standard oligonucleotides in combination (in addition to restriction enzymes in the case of AFLPs), considerably reducing the effort and consumables, and therefore...

Ngày tải lên: 09/08/2014, 18:21

... 2001) On the other hand, lapatinib can bind to the ATP-binding pocket of EGFR/HER2 protein kinase domain preventing self-phosphorylation, which in turn, resulting in the inhibition of subsequent ... into a wide range of intracellular responses MAPKs (Mitogen-activated protein kinases) are protein Ser/Thr kinases that can initiate signaling cascades in response to extracellular stimuli, and ... staining Cell cycle phase can be differentiated by measuring their DNA content, where G1 phase is 2n, S phase is between 2n and 4n, and G2 phase is 4n PI can diffuse into cell nucleus and binds...

Ngày tải lên: 10/09/2015, 09:09

... target, and the selection of aptamers with both fast and slow binding kinetics It also can be used to accurately determine the binding affinities, kinetics and specificity of aptamer sequences In ... nanomolar binding affinity and specificity towards leptin was found In the second project the use of non-SELEX to select aptamers which bind to human hemoglobin and bovine catalase protein was achieved ... the use aptamers as affinity probes in ACE include IgE, HIV type reverse transcriptase, thrombin and antithrombin III12-14 The use of aptamers in biosensors is becoming a huge area of interest in...

Ngày tải lên: 10/09/2015, 09:22

... hydrophobins Fig (Continued) in the knockout mutant hfbI –, only the respective mass peak was missing (Fig 3B) Deviating post-translational processing of hydrophobins in H jecorina strains To investigate ... hydrophobins HFBIII of H jecorina and T longibrachiatum, and SRH1 and HFB1 of T atroviride, involving signal peptides, Kex2type processing, and C-terminal amino acid cleavage (Table 2) In addition, the recorded ... H jecorina strain QM 9414 (D), and the mutant strains QM 9414 Dhfb1 (B) and QM 9414 Dhfb1Dhfb2 (A) HFB I and HFB II indicate the processed hydrophobins Whereas the HFB I peak is missing in the...

Ngày tải lên: 19/02/2014, 02:20

... included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and maintained in our laboratory ... serum-supplemented L-15 (Gibco, Grand Island, NY, USA); SK-BR-3 cells were maintained in 10% fetal bovine serum-supplemented RPMI-1640 (Hyclone); T-47D and BT-474 cells were maintained in RPMI-1640 (Hyclone) ... USA) and MCF-7 cells were maintained in 10% fetal bovine serum-supplemented Dulbecco’s modified Eagle’s medium (DMEM) (Hyclone, Logan, UT, USA); MDA-MB-231 cells were maintained in 10% fetal bovine...

Ngày tải lên: 22/03/2014, 21:20

... CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only few reports on translocation of CPPs in plant cells ... the combined use of cell permeabilizing agent and cell- penetrating peptides results in the delivery of such large sized cargoes in plant cells involving endocytic ⁄ macropinocytic pathways In conclusion, ... proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such as polyarginine...

Ngày tải lên: 23/03/2014, 07:20