cancer stem cells identification and isolation

Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of

... biosciences) and FACSDiva software version 6.1 (BD biosciences) Doublet cells were eliminated using FSC-H and FSC-W, SSC-H and SSC-W Dead cells were eliminated as 7-AAD-positive cells For the positive and ... minority of the tumor cells acts as cancer stem cells (CSCs) or tumor-initiating cells to give rise to the entire tumor mass CSCs are supposed to possess the capacity for self-renewal and the hierarchical ... E Open AccessIdentification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects

Ngày tải lên: 29/09/2020, 16:01

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Y Tế - Sức Khỏe

Identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma

... Dirks P, Eaves CJ: Cancer stem cells: an evolving concept Nat Rev Cancer 2012, 12:133 –143. 5 Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 2011, ... identified CD24 as a marker in cancer stem cells in several cancers, including pancreatic cancer [11], colorectal cancer-derived cell lines [8], and ovarian cancer [9] Cancer stem cell immunophenotype ... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous

Ngày tải lên: 05/11/2020, 01:35

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identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma

... Dirks P, Eaves CJ: Cancer stem cells: an evolving concept Nat Rev Cancer 2012, 12:133 –143. 5 Reya T, Morrison SJ, Clarke MF, Weissman IL: Stem cells, cancer, and cancer stem cells Nature 2011, ... identified CD24 as a marker in cancer stem cells in several cancers, including pancreatic cancer [11], colorectal cancer-derived cell lines [8], and ovarian cancer [9] Cancer stem cell immunophenotype ... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous

Ngày tải lên: 02/11/2022, 11:41

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DSpace at VNU: Targeting specificity of dendritic cells on breast cancer stem cells: in vitro and in vivo evaluations

... Targeting cancer stem cells is considered an important approach in cancer treatment However, the similarity between stem cells and cancer stem cells can cause mistargeting between stem cells and cancer ... selective inhibition of breast cancer stem cell-dendritic cells and mesenchymal stem cell-dendritic cells on breast cancer stem cells The results show that a mixture of DCs and CTLs effected BCSC proliferation ... drug-resistant cancer cells as well as cancer stem cells. Keywords: dendritic cells, 4T1 cell line, breast tumor, breast cancer stem cells, verapamil, drug resistance Introduction Breast cancer is

Ngày tải lên: 16/12/2017, 00:33

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Sphere-forming culture enriches liver cancer stem cells and reveals Stearoyl-CoA desaturase 1 as a potential therapeutic target

... enriching cancer stem cells (CSCs) subpopulations and the mechanism by which HCC CSCs are maintained Methods: HCC cell lines and fresh primary tumor cells were cultured in serum-free and ultra-low ... the article Trang 2HCC biology and behavior will lead to advances intreatment The cancer stem cell (CSC) hypothesis posits that a small subset of cancer cells, with stem cell-like proper-ties, has ... rounds of spheres and differentiated cells The three sets of HCC spheres and differentiated sphere cells were harvested and RNA was extracted for PCR analysis RNA extraction and quantitative RT-PCR

Ngày tải lên: 17/06/2020, 17:05

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Long intergenic non-protein-coding RNA 1567 (LINC01567) acts as a “sponge” against microRNA-93 in regulating the proliferation and tumorigenesis of human colon cancer stem cells

... in regulating the proliferation and tumorigenesis of human colon cancer stem cells Xiaofeng Yu1, Lin Mi1, Jie Dong2and Jian Zou1* Abstract Background: Cancer stem cells (CSCs) are considered to ... fragments with the corresponding primers (primers 1 and 20, 19 and 40, 39 and 60, 59 and 80, 79 and 100, 99 and 120, 119 and Trang 5140, 139 and 162) The 50 μL PCR reaction mixture in-cluded 5μL ... years A small group of cells with stem cell properties, has been sepa-rated from CRC and they are referred to as CRC stem cells (CR-CSCs) [2] These cells proliferate infinitely and differentiate into

Ngày tải lên: 06/08/2020, 04:42

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β-arrestin1-mediated acetylation of Gli1 regulates Hedgehog/Gli signaling and modulates self-renewal of SHH medulloblastoma cancer stem cells

... including a subset of cells with stem-like character-istics called “cancer stem cells” (CSCs) CSCs could trigger tumor formation, drive resistance to conventional therapeutics and underlie patient ... miR-326 and Arrb1 that leads to their differentiation and loss of stemness markers Second, Arrb1 and miR-326 converge upon the Hh/Gli downstream signaling pathway that regulates cell growth and stemness ... and repeated at least three times HEK293T cells were cultured and transfected as previsouly described [26] with the indicated plasmids as in [31] Western blot and immunoprecipitation assays Cells

Ngày tải lên: 06/08/2020, 06:22

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Chemotherapy curable malignancies and cancer stem cells: A biological review and hypothesis

... somatic stem cell, the cancer stem cell and the cancer cells is relatively simple with the tissue specific stem cell giving rise through mutation to the cancer stem cell and the cancer stem cells ... Hierarchical Cancer Stem Model In this simplified model, the standard somatic stem cells, give rise to healthy cells and also maintain their stem cell pool In the epithelial cancers the somatic stem cells ... structure of the cancer stem cell is the hierarchical system as shown in Fig 1 In this system the cancer stem cells arise from somatic stem cells and give rise to their progeny of malignant cells which

Ngày tải lên: 20/09/2020, 18:51

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Essential role of miR-200c in regulating selfrenewal of breast cancer stem cells and their counterparts of mammary epithelium

... Abstract Background: Breast cancer stem cells (BCSCs) have been reported as the origin of breast cancer and the radical cause of drug resistance, relapse and metastasis in breast cancer BCSCs could be ... (FACS) and tested for stemness by clonogenic assay and multi-potential differentiation experiments The miRNA profiles of MaSCs, BCSCs and breast cancer MCF-7 cells were compared to obtain the candidate ... the stemness of BCSCs and MaSCs Keywords: miR-200c, Breast cancer, Cancer stem cells, Carcinogenesis, Self-renewal, PDCD10, Malignant transformation * Correspondence: sunjg09@aliyun.com 1 Cancer

Ngày tải lên: 28/09/2020, 01:41

12 26 0

Y Tế - Sức Khỏe

Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells

... multipotent stem cells and that their genetic composition might be valuable for future gene and stem cells therapy for lung cancer Keywords: Cancer stem cells, Non-small cell lung cancer, Cell ... Trypsin-EDTA when they reached 80% confluence Isolation of putative CSCs and normal stem cells The lung cancer cells and normal cells were detached with trypsin and washed with phosphate buffer solution ... behaviour of cancer cells is driven by a subpop-ulation of cells within the tumour called cancer stem cells (CSCs) [4,5] CSCs were first described in human hematopoietic cancer, and to date they

Ngày tải lên: 30/09/2020, 13:56

16 11 0

Y Tế - Sức Khỏe

Protein kinase C-delta inactivation inhibits the proliferation and survival of cancer stem cells in culture and in vivo

... including “cancer progenitor cells,” “cancer stem cell-like cells,” and “cancer-initiating cells,” but the term “cancer stem cell” (CSC) has received wide acceptance [6] The first identification ... self-renewing and anchorage-independent sphe-roids were obtained by culturing breast cancer cells MCF7, Hs587T and MDA231; melanoma cells SBcl2 and FM6; human breast cancer stem cells and pancreatic ... prostate cancer [13], ovarian cancer [14-18], gastric cancer [19], lung cancer [20,21], colon cancer [22-25], pancreatic cancer [26,27], melanoma [28-30], head and neck cancer [31], and others

Ngày tải lên: 05/11/2020, 01:11

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targeting-cancer-stem-cells-and-metastasis-with-epigenetic-modulation-and_COR-2020-7-104

... subset of tumor cells contributes to long-term tumor growth These cancer cells have been termed tumor-initiating cells or cancer stem cells (CSCs) Purpose: Considerable preclinical and clinical ... on ice Cells were spun and washed once with dPBS Cells were resuspended in 100µL dPBS and 5µL CD24-FITC, 5µL CD44-APC, and 5µL CD49f-PE Cells were incubated for 15 minutes at 37°C, spun, and washed ... vorinostat and lapatinib is safe and active in HER2-positive breast cancer Introduction A wealth of data supports the hypothesis that cancer cells are heterogeneous in their proliferative capacity, and

Ngày tải lên: 22/10/2022, 22:54

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cancer stem cells in basic science and in translational oncology can we translate into clinical application

... on mye- loid progenitor cells and endothelial cells, and KIT is not only expressed on hematopoietic stem- and progenitor cells but also on mast cells, germ cells, and melano- cytes [70,71] Therefore, ... however, stem cells do not undergo redis- tribution and homing, unless these cells transform to metastasizing CSC Stem cell homing of normal hematopoietic stem cells and LSC is a multi-step process and ... contribute to stem cell niche teractions and stem cell self-renewal in normal and (pre) malignant conditions [225]. in-Stem cell homing and abnormal spread of NSC/CSC Depending on the organ system, homing

Ngày tải lên: 02/11/2022, 09:03

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identification of a distinct population of cd133 cxcr4 cancer stem cells in ovarian cancer

... -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4 + CD133 + within ovarian cancer ... concepts in clinical targeting of cancer stem cells Stem Cells 29, 883–7 (2011). 34 Zhou, B.B et al Tumour-initiating cells: challenges and opportunities for anticancer drug discovery Nat Rev Drug ... CD133 + ovarian cancer cells display stem cell properties Since CXCR4 and CD133 were previously reported as stem cell markers27,28, the OVCAR-3, OVCAR-4 and OVCAR-5 ovar-ian cancer cell lines

Ngày tải lên: 04/12/2022, 10:37

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nitrative and oxidative dna damage in infection related carcinogenesis in relation to cancer stem cells

... may generate mutant stem cells and cancer stem cells, leading to carcinogenesis Indeed, 8-nitroguanine was formed in stemness marker-positive cells in parasite-associated cancer tissues The mechanism ... SOX2, Klf4, and c-myc [38] It is necessary to study whether 8-nitroguanine forms in cancer stem cells Human papillomavirus and cervical cancer Cervical cancer is the fourth most common cancer among ... co-localization of DNA damage marker and stemness marker in cancer cells Original magnification is × 400; Scale bar = 25 μm Trang 10cells, leading to cancer stem cells Inflammation by OVinfection

Ngày tải lên: 04/12/2022, 15:37

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cancer stem cells theories and practice_part1 pps

... Stem Cells to Overcome Drug Resistance 401 Miaorong She and Xilin Chen Chapter 21 Cancer Stem Cells and Chemoresistance Suebwong Chuthapisith Chapter 22 Cancer Stem Cells in Drug Resistance and ... Chapter Prostate and Colon Cancer Stem Cells as a Target for Anti -Cancer Drug Development Galina Botchkina and Iwao Ojima Niches and Vascularization 135 155 Importance of Stromal Stem Cells in Prostate ... Cruz and Isidro Sanchez-Garcia Part Stem Cells in Specific Tumors 61 Chapter Breast Cancer Stem Cells 63 Marco A Velasco-Velázquez, Xuanmao Jiao and Richard G Pestell Chapter Glioma Stem Cells: ...