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... 5¢-CCCGCCATGGGTAAAATAATTGGTA TCG-3¢ and 5¢-CCCGGATCCAAGCTTTTACTGCTTAG TTTCACCAGA-3¢ The PCR fragments were cloned into the bacterial expression vector pTrc99A (Amersham Phar-macia Biotech, Piscataway, NJ) ... which are similar to those of KKCC and KCCC and clearly distinct from those of DnaK, suggest that the stabilizing A B C D E Fig 5 KKCC and KCCC tryptic sites have an altered topology Coo-massie ... mitochondria, and characterized their bio-chemical and biophysical properties In addition, we studied the chimeras KKCC and KCCC constructed by domain swapping [18] (Fig 1A) While maintain-ing the ATPase

Ngày tải lên: 16/03/2014, 22:20

... Trang 1Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages Yuichiro Otsuka1, ... hyphae fraction; EC, extracellular frac-tion; CY, cytoplasmic fraction Control, GOU added to 1 mL of VM (B) Assay of b-aryl ether cleavage by the extracellular fraction with a model compound that ... prepared a hyphae fraction (HP), an extracel-lular fraction (EC), a cytoplasmic fraction (CY) and a membrane fraction (M) from cultures of 2BW-1 (see Materials and methods) Enzymatic activity was determined

Ngày tải lên: 17/03/2014, 03:20

... Proceedings of the 43rd Annual Meeting of the ACL, pages 280–289, Ann Arbor, June 2005. c 2005 Association for Computational Linguistics QARLA:A Framework for the Evaluation of Text Summarization ... Related work and discussion 6.1 Application of similarity metrics to evaluate summaries Both in Text Summarisation and Machine Trans- lation, the automatic evaluation of systems con- sists of computing ... Q M,x (a) that estimates the quality of an automatic summary a ∈ A, given a set of models M and a similarity metric x. An obvious first attempt would be to compute the average similarity of a to all

Ngày tải lên: 17/03/2014, 05:20

... Trang 1domain of yeast seryl-tRNA synthetase and forms aVlatka Godinic1, Marko Mocibob1, Sanda Rocak1, Michael Ibba2and Ivana Weygand-Durasevic1 1 Department of Chemistry, Faculty of Science, ... of Zagreb, Croatia 2 Department of Microbiology, The Ohio State University, Columbus, OH, USA Accurate aminoacylation of tRNA by aminoacyl-tRNA synthetases (aaRSs) is a crucial step in the faithful ... mays; CA, Candida albicans; SC, S cerevisiae; SP, Schizosaccha-romyces pombe Only eukaryotic cytosolic enzymes contain positively charged C-terminal extensions The C-terminal sequence of S cerevis-iae

Ngày tải lên: 23/03/2014, 09:20

... that MCA2590 is a member of a novel group of bacterial di-heme cytochrome c peroxidases not previously characterized Abbreviations BCCP, bacterial cytochrome c peroxidase; CCP, cytochrome c peroxidase; ... in accordance with the prediction of a signal peptide in a primary translation product Bioinformatical analyses revealed that SACCP shares characteristics with previously described mem-bers of ... increased in bacteria cultured at low-copper concentrations The protein is located on the surface of the bacterium and is noncovalently attached to the outer membrane The extracellular localization

Ngày tải lên: 23/03/2014, 11:20

... separate the cytosolic fraction and the membrane fraction Purification VcOadA-2, VcOadA-2-C, and VcOadG¢A-2 and its derivatives by monomeric avidin-Sepharose affinity chromatography The plasmids ... Taralczak M, Wild M & Dimroth P Oxaloacetate decarboxylase of Vibrio cholerae: purification, characterization and expression of the genes in Escherichia coli Arch Microbiol in press 22 Schneider ... sequences of various oxaloacetate decarboxylase containing organisms and found that V cholerae contains the genes for two different oxalo-acetate decarboxylases, named OAD-1 and OAD-2, which both

Ngày tải lên: 23/03/2014, 13:20

... proline rich domain. The quaternary associations of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are determined by small C-terminal domains that are distinct from the catalytic domain ... °C, in a LE80K centrifuge using an SW-41 rotor (Beckman–Coulter, Villepinte, France). Fractions of 300 lL were collected and assayed for AChE, b-galactosidase and alkaline phosphatase activities. ... Jean Massoulie ´ 1 1 Laboratoire de Neurobiologie Cellulaire et Mole ´ culaire, Ecole Normale Supe ´ rieure, Paris, France; 2 Centre de Recherche Paul Pascal, Pessac, France Acetylcholinesterase

Ngày tải lên: 30/03/2014, 13:20

... the antenna-element k; and (a) Two antenna elements of an antenna array at the base station. (b) The V-array: an antenna array with 3 antenna elements at the base station. (c) An antenna array ... angular distribution and the desired accuracy of the AS estimation. A. Known angular distribution type and low AS estimation accuracy for small AS values Letusfirststudyasimplecase.Considerapairof ... V- array: an antenna array with three antenna-elements at the base station. c An antenna array with three antenna-element. d Butterfly configuration. Bousnina et al. EURASIP Journal on Advances

Ngày tải lên: 20/06/2014, 22:20

... Serologicals (Norcross, GA) The primer sequences used for real-time PCR are as follows: FIV reverse-transcriptase forward: 5'-ACTGAACCTGAC-CGTACAGATAAATTACAGGAA GAACCCCCATA-3' FIV reverse-transcriptase ... Euthanasia of research animals was conducted with strict adherence to NIH Office of Lab-oratory Animal Welfare protocols Blood Collection and Peripheral Blood Separation Blood samples (1 ml/animal) ... treatment has been the use of specific inhibitors of the viral aspartic pro-tease family as part of a drug cocktail, called highly active anti-retroviral therapy (HAART), with the ultimate goal

Ngày tải lên: 13/08/2014, 13:20

... breast meat characteristics was carried out This failed to achieve convergence probably because of the high estimated genetic correl-ations between the C and H parameters and the a∗ and b∗ parameters ... to achieve convergence and later did not provide standard errors of the estimates As for the breast meat characteristics, the global analysis for the thigh meat characteristics failed to achieve ... being associated with a decreased water-holding capacity and a pale colour [2, 3], and a high pH with a poor storage quality [1] Meat quality is influenced by a large number of factors, including

Ngày tải lên: 14/08/2014, 13:22

... proposal of establishing medical equipment and medicine list, combat basic loads and methods of guaranteeing medical materials for K20 The practical basis of the task of guaranteeing medical materials, ... SPECIAL TASK FORCE 4.2.1 Regarding methods of calculation and establishment of list contents and basic load packaging method Particularly we have built: - Adequate and fundamental list of medicines ... medical standards and norms, professional regulations - Didactic materials on guaranteeing ordnance task in the Military, on organizing command of military health and military medical ordnance

Ngày tải lên: 29/09/2014, 16:23

... always exists when the products have a potential market Apart from available advantages such as absolute cost, and the capacity of approaching the input materials, understanding cycles of coating ... manufacturers and maintenance providers for the commercial, military, regional jet and general aviation industries Services include chemicals management Architectural Coatings Americas and Asia/Pacific: ... units, etc both create initial chances and challenges for business process of an enterprise In case of fruitful resources and favorable geographical location, competition capacity of the company would

Ngày tải lên: 24/11/2014, 01:04

... method of communication into social and communal affairs Besides, we can also list and analyze the possible limitations and advantages of nonverbal communication for social communication In chapter ... significance of nonverbal communication in conducting social activities, scientific comparisons of verbal and nonverbal communication indicated that the vast majority of conversational messages communicated ... out Finally, nonverbal communication can also regulate the verbal communication In association with the backchannel communication, there are a lot of rules that nonverbal communication can help

Ngày tải lên: 28/02/2015, 11:54

... performance appraisal is discovered as a socio-emotion factor affect job satisfaction Performance appraisal is a process by which a superior evaluates and judges the work performance of a subordinate ... satisfaction, how employee feel of job performance appraisal is a special feeling which may cause emotional satisfaction or dissatisfaction In a research of Marie (2003), perceived-fairness of ... Standard Chartered : Standard Chartered Bank Ltd Vietcombank : Vietnam Commercial Bank Prufinance : Prudential Finance Joint Venture Ltd SPSS : Computer Statistic Package for Social Science Trang

Ngày tải lên: 14/01/2016, 18:16

... Aesthetics needs: Base on the specific characteristics of the airport, there are three main kinds of accessing flows such as: passenger accessing flows, cargo accessing flows as well as luggage accessing ... that constitute a servicescape These stimulate are characterized as being organizationally controllable and able to enhance or constrain employee and customer approach/avoidance decisions and ... using Cronbach’s Alpha to examine reliability and Confirmatory Factor Analysis (CFA) to check validity value of the scale measurement as well as determine the adequacy of model fit to the data In

Ngày tải lên: 11/05/2017, 10:06

... Scales in this research were evaluated by factor loadings indicators, Cronbach’s alpha indicators, composite reliability (CR) and the average variance extracted (AVE) in confirmatory factor analysis ... Therefore, creating the organizations’ reputation is crucial to take advantages the new trend of direct booking channel The main characteristic of a service sector is “the contact and interaction ... Standardized coefficient path ……… 24 LIST OF TABLES Table 1 Respondents’ characteristics 17 Table 2 Means, standard deviations, and standardized CFA loadings of items 19, 20 Table 3 Correlations

Ngày tải lên: 03/08/2018, 23:29

... typecharcan be declared like this: char v[6]; // array of 6 characters Similarly, a pointer can be declared like this: char∗ p; // pointer to character In declarations,[ ] means ‘‘array of’’ and∗means ... ofanswerappears where it is needed (and not before that) A declara-tion can appear anywhere a statement can Trang 24Aswitch-statement tests a value against a set of constants The case constants ... point of declaration to the end of the block in which its declara-tion occurs A block is delimited by a { }pair Function argument names are considered local names • Class scope: A name is called a

Ngày tải lên: 05/10/2018, 12:50

... typecharcan be declared like this: char v[6]; // array of 6 characters Similarly, a pointer can be declared like this: char∗ p; // pointer to character In declarations,[ ] means ‘‘array of’’ and∗means ... ofanswerappears where it is needed (and not before that) A declara-tion can appear anywhere a statement can Trang 24Aswitch-statement tests a value against a set of constants The case constants ... point of declaration to the end of the block in which its declara-tion occurs A block is delimited by a { }pair Function argument names are considered local names • Class scope: A name is called a

Ngày tải lên: 12/03/2019, 16:45

... Trang 1www.thenzbplace.comTrang 2www.thenzbplace.comTrang 4www.thenzbplace.comTrang 17www.thenzbplace.com

Ngày tải lên: 06/09/2019, 16:06

... fabric of organization and the doctrinal, and theological regulations of local church organization The relationship between Christian and Missionary Alliance (C&MA) and the French Protestant Church ... primarily characteristics1 Trang 371 The policy of “Local Church” was approved by A B Simpson and was accepted as an essential principle in the foreign missionary areas This policy is also called ... requirements of Church, including the construction of churches and other welfare projects Self-propagation is that when local churches have enough capacity of people and materials, they have to evangelize

Ngày tải lên: 10/01/2020, 19:33