a finitestate model of human

... biological mechanisms underlying human gastric cancer metastasis In this paper, we established a mouse model of metastatic human gastric cancer in nude mice that has a higher rate of tumor formation ... Huifen Du1, Xiaowen Lian1, Dandan Chai1, Xinwen Li2, Rong Yang3 and Chunya Wang1 Abstract Background: A mouse model of metastasis of human gastric cancer is one of the most important tools for ... Li et al BMC Cancer (2016) 16:54 DOI 10.1186/s12885-016-2101-z RESEARCH ARTICLE Open Access Establishment and characterization of a metastasis model of human gastric cancer in nude mice

Ngày tải lên: 21/09/2020, 11:27

... 5'-GCCTCCAGCATGAAAGTCTC, 3'-TAAAACAGGGT-GTCTGGGGA), IL-1β (5'-TGCACGATGCACCTGTACGA, 3'-AGGCCCAAGGCCACAGGTAT), IL-6 (5'-GTTCCTGCA-GAAAAAGGCAAAG, 3'-CTGAGGTGCCCATGCTA-CATTT), matrix metalloproteinase (MMP)-3 ... (5'-GTCCTCTC-CCAAGTCCACACA, 3'-CTGGTCTCAAGTCAGTGTACAG-GTAA), CC chemokine ligand (CCL)5 (formerly called RANTES; 5'-CGCTGTCATCCTCATTGCTA, 3'-GCT-GTCTCGAACTCCTGACC), CCL2 (formerly called MCP-1; 5'-GCCTCCAGCATGAAAGTCTC, ... affects nearly 1% of the population worldwide and can lead to significantly impaired quality of life Mortality rates are also significantly increased in patients with RA, and currently available

Ngày tải lên: 09/08/2014, 07:20

... 24:1009-1034 43 Takimoto M, Hamada A, Tomoda A, Ohdo S, Ohmura T, Sakato H, Kawatani J, Jodoi T, Nakagawa H, Terazono H, Koyanagi S, Higuchi S, Kimura M, Tukikawa H, Irie S, Saito H, Miike T: Daily expression ... streptavidin phycoerythrin and scanned on the Agilent Gene Array Scanner™ (Agilent Technologies) Analysis of microarray data We compiled a database that includes 38 Focus GeneChip® microarrays (Affymetrix) ... synthesis pathways Two additional pathways, a lipid metabolism pathway, and a cellular assembly and organization pathway, included, respectively, 71- and 68-TIR gene matches The top matching module

Ngày tải lên: 13/08/2014, 21:21

... hindlimb at grade and I after cell injection Conclusion: Thus, xenotransplantation of human ADSCs might play a significant role in the formation of new blood vessel and can assist in the treatment of ... associated with the activation of natural killer cells and the maturation of dendritic cells ADSCs also increase the rate of synthesis of regulatory T cell associated with the modulation of the ... et al., 2000; Strem et al., 2005) They also play an important role in repairing damaged tissues The role of ADSCs was demonstrated in the same way as that of bone marrow-derived mesenchymal stem

Ngày tải lên: 16/12/2017, 18:01

... artificial language can be characterized in terms of the phrasestructure rules: ii X 3 aXa X 3 bXb X 3 $ % which generate the strings: $ %, aa, bb, abba, baab, aaaa, bbbb, aabbaa, abbbba, We call ... Vogel, Hahn & Branigan(1996) to define the third artificial language in terms of the following rule set: which generates the strings: $ %, aa, bb, abab, aaaa, bbbb, aabaab, abbabb, Trang 6We ... training grammar in making its predictions, taking hits, false alarms, correct rejections and misses intoaccount Hits and false alarms are calculated as the accumulated activations of the set of

Ngày tải lên: 12/10/2022, 20:53

... Visualization Laboratory at the University of Michigan, Ann Arbor, MI Results Trang 7Startup When the system is started in a random initial state, a transient characteristic of the initial state appears ... where all of its cells are at Trang 8Pacemaking Started in a random state with a single pacemaking cell located in the uterine fundus and an arbitrarily determined fraction of resting cells, all models ... organization appears as seen in pressure generation scatterplots (Figure 2) Activated areas collide, propagate, and are annihilated Vortices or rotors appear at various locations on the surface

Ngày tải lên: 18/10/2022, 22:05

... CCTGCCCGACCCTACAAAAT-3′​ and reverse primer 5′​ - TCCGCCGTCTTAAGGTAGGA-3′, product size: 313 bp; Nkx2.5: forward primer 5′​-CAATGCCTATGGCTACAACGC-3′​ and reverse primer 5′​ - GAGTCATCGCCCTTCTCCTAAA-3′​, ... in male C57BL/6 mice (Shanghai Slaccas laboratory Animal Center, China) and CAG-EGFP transgenic mice (Shanghai Biomodel Organism, China) at age of 6–8 weeks (20–25 g) as described33 Briefly, after ... decellularized hHVS can be used as a biocompatible biomaterial for translational cardiac tissue engineering As the availability of allogeneic valve scaffolds is also limited, future work may focus

Ngày tải lên: 04/12/2022, 10:40

... Trang 1downmodulation and a decreased migration of humanhepatocellular carcinoma cells Alessandro Salvi1, Cristiano Sabelli1, Silvia Moncini2, Marco Venturin2, Bruna Arici1, Paola Riva2, Nazario ... development and progression of several types of malignant tumor An essential role of uPA and c-met in the migration and proliferation of human hepatocellular carcinoma (HCC) cells has been assessed ... 2A,B, real-time RT-PCR and northern blot data revealed that miR-23b was highly expressed in AB2 human dermal fibroblasts, whereas it was detectable at lower and variable levels in five human HCC-derived

Ngày tải lên: 29/03/2014, 23:20

... probabilistic Combinatory Categorial Grammar (CCG) framework, and assume only that the learner has access to a small set of general combinatory schemata and a functional mapping from semantic ... syn-tactic productions are allowed in the grammar: START→ A to generate A as the top syntactic node of a parse tree and A→ [A]lex to indicate that A is a leaf node in the syntactic derivation and ... (2010) Learning general properties of semantic roles from usage data: a computational model Language and Cognitive Processes, 25:1 Beal, M J (2003) Variational algorithms for ap-proximate Bayesian

Ngày tải lên: 31/03/2014, 20:20

... 105) at passage 6 were transplanted into the cisterna magna of 10 ischemic rats seven days after the Trang 5induction of focal cerebral ischemia Transplanted ani-mals did not behave abnormally ... Transplantation Seven days after the induction of focal cerebral ischemia, animals were anesthetized with an intra-peritoneal injec-tion of 400 mg/kg chloral hydrate (Pharmaceutical Plant of ... occlusion of a cerebral artery with thrombi and emboli, which leads to an infarction and the death of neural tissue Current treatments are pri-marily palliative and are useful to only a minority of patients

Ngày tải lên: 18/06/2014, 16:20

... Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China Full list of author information is available at the end of the article © 2011 Xu et al; ... 30672438) and the Hubei Provincial Natural Science Foundation of China (Nos 2006ABC009 and JX4A06). Author details 1 Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, ... toxicity, as shown by serum analysis for biochemical markers of liver and kidney and the histological examination of hematoxylin and eosin stained major organs (Figure 5) Taking all these data into

Ngày tải lên: 18/06/2014, 19:20

... interface of ablated and distant tumors, at the abla-tion injury front and within vascular lakes of ablated and distant tumors These increases persisted at high levels compared to shams at all ... mice had sham TA performed (LA probe was not activated) Panels a and b tumor sections from control (sham ablated) and LA treated animals at day 5 post treatment respectively, stained with rat anti ... post ablation from areas indicated in the diagram of panel a Control mice had sham LA performed (LA probe was not activated) Panels b-d are CD3+ stained tissues from control animals; b liver parenchyma,

Ngày tải lên: 18/06/2014, 19:20

... included anti-VEGF (Avastin, Genetech, San Francisco, CA), anti-TNFa (Humira, Abbott, Abbott Park, IL), anti-IL-1b (clone AB-206-NA, Abcam, Cambridge, MA), anti-IL-6 (clone AB-201-NA, Abcam), anti-GM-CSF ... MDSC samples (4-998 osteogenic sarcoma, DU 145 prostate carcinoma, CAKI-1 renal cell carcinoma, SKOV-3 ovarian carcinoma, and SW 608 and SW 732 colorectal adenocarcinoma cell lines), we analyzed ... Biotec) and used for functional studies Arginase activity was measured in cell lysates using Bioassay Systems’ QuantiChrom Arginase Assay Kit (Hayward, CA) per the manufacturer instructions For measurement

Ngày tải lên: 18/06/2014, 19:20

... Rüttinger1†, Margareta Rusan1, Robert Kammerer2, Wolfgang Zimmermann3, Rudolf A Hatz1and Hauke Winter1 Abstract Background: Active-specific immunotherapy used as an adjuvant therapeutic strategy is rather ... Trang 1R E S E A R C H Open AccessCombination immunotherapy and active-specific tumor cell vaccination augments anti-cancer immunity in a mouse model of gastric cancer Natasja K van den ... were analyzed in duplicate Extinction was analyzed at 405/490 nm on a TECAN microplate ELISA reader (TECAN, Crailsheim, Germany) with the EasyWin software (TECAN) The detection limit of the ELISA

Ngày tải lên: 18/06/2014, 22:20

... Microbrightfield software and cell density was calcu-lated for striatal and midbrain volumes The striatum was outlined according to anatomical landmarks following the Paxinos atlas[31] To avoid bias in the ... weeks after the striatal lesion All ani-mals showed a marked asymmetry (~90% of the contacts were made using the ipsilateral paw) Histological and stereological procedure Animals were terminally anesthetized ... Neuroregeneration Laboratories, McLean Hospital, Belmont, Massachusetts, USA and 3 Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts, USA Email: Rosario Sánchez-Pernaute

Ngày tải lên: 19/06/2014, 22:20

... using Axiovision 3.1 software Digital images were analyzed using KS300 analysis program (Zeiss) Percentage of immunostained area (area of immunostaining/total image area × 100) was Table 1: Summary ... that reactive microglia are characteristi-cally associated with fibrillar amyloid containing plaques in both human AD and animal models, we assessed the effect of immunization on microglial reactivity ... Single ANOVA statistical analysis was used to assess the significance of the differences in plaque area, glial and C3 activation products reactivity among the animals groups A second method of quantification

Ngày tải lên: 19/06/2014, 22:20

... ggaggaatgggagtt-gctgttgaa-3'; iNOS, forward primer, 5'-ggaagaggaacaactactgctggt-3', reverse primer, 5'-gaactgaggg-tacatgctggagc-3' Thermal cycling conditions were as fol-lows: activation of iTaq DNA polymerase at ... homeothermic blanket control unit (Harvard Apparatus, Holliston, MA) Cranial temperature was measured by HSE Plugsys TAM-D (Harvard Apparatus) Blood gases and blood pH were measured by pH/blood gas analyzer ... external carotid artery (ECA) was isolated and ligated A 4-0-monofilament nylon suture (Harvard Apparatus, MA) was inserted through the ECA into the internal carotid artery until a mild resistance

Ngày tải lên: 19/06/2014, 22:20

... neurodegenerative disorders Other authors have documented that pharmacological activation of KATP channels can exert neuroprotective and anti-inflammatory effects on the brain against ischemia, trauma and ... reduction assay was used as an indicator of cell viability MTT (Sigma-Aldrich, St Louis, MO, USA) was added to a well at a final concentration of 0.5 mg/mL After MTT incubation at 37°C, DMSO was added ... EAE animals Diazoxide treatment diminishes the astrocytic reactivity and area of activated microglia/macrophage in the effector phase of EAE To assess the consequences of diazoxide administration

Ngày tải lên: 19/06/2014, 22:20

... Program of Shanghai Municipality (S30202), Shanghai Key Project of Basic Science Research (10411954500), and Program for Outstanding Medical Academic Leader of Shanghai (LJ 06003) Author details ... striatum may spread into the SN, causing abnormal activation of Zhang et al Journal of Neuroinflammation 2011, 8:154 http://www.jneuroinflammation.com/content/8/1/154 microglia and damage to dopaminergic ... counts of contralateral rotation and a significant decrease in TH-immunoreactive (TH-ir) neurons in SN A marked decrease in monoamine levels was also detected in 6-OHDA/BAb-treated rats, in comparison

Ngày tải lên: 19/06/2014, 22:20