3 immune modulation by hematopoietic stem cells

... progenitor proliferation Hematopoietic stem cells have governing mechanisms that are distinct from the cells they generate Hematopoietic Stem Cell Differentiation Hematopoietic stem cells sit at the ... location of stem cells by indirect means, altering molecules such as CXCL12 that tether stem cells to their niche Molecules shown to be important for altering the proliferation of stem cells, such ... changes in the cells Our knowledge of the details remains incomplete (see http://stemcell.princeton.edu/ for a comprehensive listing of gene expression in stem cells) As stem cells mature to...

Ngày tải lên: 07/07/2014, 01:20

... these cells Given the possibility that both hematopoietic stem cells and EPC may serve as endogenous “repair cells , we sought to assess a relatively non-invasive means of modulating these cells Stem- Kine ... human stem cells Stem Cells Dev 2006, 15(1):118-23 77 Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: ... 50 cells circumscribed by spindle shaped cells and were counted by microscope As the number of colonies depends on the number of plated cells, normalization of colony number based amount of cells...

Ngày tải lên: 18/06/2014, 16:20

... induction of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such ... relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in this study the phosphoproteome ... and culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells (MNCs) from...

Ngày tải lên: 18/06/2014, 19:20

... much of what we know about stem cells is imprecise and based on inferences from genetically manipulated animals Cardinal Functions of Hematopoietic Stem Cells All stem cell types have two cardinal ... accompanying functional changes in maturing cells, aggregating cells into functional subgroups, and demonstrating hematopoietic stem cell regulation by a specialized microenvironment are concepts ... mature cells Without proper differentiation, the integrity of tissue function would be compromised and organ failure would ensue Figure 68-1 Signature characteristics of the stem cell Stem cells...

Ngày tải lên: 07/07/2014, 01:20

... The presence of stem cells in the circulation is not unique to a time window in development Rather, hematopoietic stem cells appear to circulate throughout life The time that cells spend freely ... or a different host Mobility of Hematopoietic Stem Cells Cells entering and exiting the bone marrow so through a series of molecular interactions Circulating stem cells (through CD162 and CD44) ... release of stem cells into the circulation This process is an increasingly important aspect of recovering stem cells for therapeutic use as it has permitted the harvesting process to be done by leukapheresis...

Ngày tải lên: 07/07/2014, 01:20

... capacity, stem cells have an additional feature characterizing their proliferation machinery Stem cells in most mature adult tissues are deeply quiescent In the hematopoietic system, stem cells are ... effectively expand human hematopoietic stem cells The process may be controlled by particularly high levels of expression of cyclindependent kinase inhibitors that restrict entry of stem cells into cell ... important function of stem cells because it is critical in regulating the number of stem cells Stem cell number is a key limiting parameter for both autologous and allogeneic stem cell transplantation...

Ngày tải lên: 07/07/2014, 01:20

... Else Can Hematopoietic Stem Cells Do? Some experimental data have suggested that hematopoietic stem cells or other cells mobilized into the circulation by the same factors that mobilize hematopoietic ... experimentally in the hematopoietic system Taking advantage of the cell-surface markers that distinguish hematopoietic cells of varying maturity, stem cells, progenitors, precursors, and mature cells can ... the use of the stem cells and the use of drugs to target stem cells to enhance repair of damaged tissues It will also include the careful balance of interventions to control stem cells where they...

Ngày tải lên: 07/07/2014, 01:20

... [9-18] Leukemia Stem Cells/ Cancer Stem Cells (LSC/CSC) The cancer stem cell hypothesis postulates that cancer can be initiated, sustained and maintained by a small number of malignant cells that have ... Leukemic Stem Cells Three hypotheses have been proposed that lead to the development of leukemic stem cells (LSC/CSC): (A) LSC/CSC might arise from either a hematopoietic stem cell (HSC), hematopoietic ... specialized cells of the hematopoietic system HSCs differentiate into two distinctive types of hematopoietic progenitors: 1) a common lymphoid progenitor (CLP) population that generates B -cells, T-cells...

Ngày tải lên: 12/08/2014, 23:23

... these cells migrate here from other hematopoietic sites, or whether they arise in situ from resident stem or progenitor cells, though it has been hypothesized that the PBI is generated by migration ... debris In addition, cells of the lymphoid lineage [T -cells, B -cells and natural killer (NK) -cells] mediate specific defences against pathogens and the elimination of abnormal cells (tumours) Erythrocytes ... from a rare population of cells in the BM known as the HSC These stem cells represent a self-renewing population of cells that have the potential to generate progenitor cells that differentiate...

Ngày tải lên: 11/09/2015, 09:10

... of helminth-induced immune modulation on bystander responses Helminth infections induce type immune responses which are characterized by the induction of T helper cells, eosinophilia and elevated ... of systemic inflammation, usually triggered by bacteria and their toxins, although fungi- and virus-induced sepsis also exists Sepsis is a severe, lifethreatening condition characterized by pathophysiological ... severity was determined by survival, development of hypothermia, systemic proinflammatory cytokine and chemokine levels Clearance of bacteria and recruitment of immune cells to the peritoneum...

Ngày tải lên: 26/11/2015, 10:00

... these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression of GR-1, CD4/8, B220 and Sca-1 in BM cells respectively ... in CD34 positive hematopoietic stem/ progenitor cells [16] To further test the effect of Sall4 in LSK cells, we performed a Sall4 knockdown study in isolated mouse BM LSK cells by an RNA interference ... IL) Blots were detected by a Kodak Image Station 2000 MM (Kodak, Rochester, NY) Isolation of mouse BM hematopoietic stem/ progenitor cells Cells were isolated from whole BM by immunostaining with...

Ngày tải lên: 10/08/2014, 21:23

... mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem ... mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell Res ... ischemic heart by Akt-modified mesenchymal stem cells Nat Med 11, 367–368 Kinnaird T, Stabile E, Burnett MS, Lee CW, Barr S, Fuchs S & Epstein SE (2004) Marrow-derived stromal cells express genes...

Ngày tải lên: 18/02/2014, 04:20

... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... differentiation Mouse ES cells were differentiated to generate cells of the hematopoietic lineage using the ES ⁄ OP9 method established and described by Kitajima et al [20] Briefly, ESD3 cells (ATCC, CRL-1934) ... Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes...

Ngày tải lên: 07/03/2014, 12:20

... of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived ... profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos ... By contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells...

Ngày tải lên: 23/03/2014, 07:20

... from the muscle by homogenization than IMF mitochondria In a first attempt to prepare IMF mitochondria, we used the original technique of Krieger et al [11], which involves digestion by the Nagarse ... even when tightly controlled, was found by Western blot analysis to degrade UCP3 Therefore we adopted another technique in which we broke down myofibrils by strong mechanical disruption with a ... peroxidase-labeled secondary antibody The signals were detected by chemiluminescence using a standard ECL kit and developed Hyperfilm ECL film They were quantified by scanning photodensitometry using ImageQuant...

Ngày tải lên: 24/03/2014, 04:21

... orders@intechweb.org Neural Stem Cells and Therapy, Edited by Tao Sun p cm ISBN 978-953-307-958-5 Contents Preface IX Part Chapter Characterization of Neural Stem Cells Neural Stem Cells from Mammalian ... (slowly dividing astroglial cells and the bona fide adult neural stem cells) , type C cells (transit-amplifying cells derived from asymmetric division of Type B cells) , type A cells (immature neuroblasts ... glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells Eur J Neurosci, Vol 18, No 1, (July...

Ngày tải lên: 30/03/2014, 23:20

... related to modulation of the class I MHC, mediated by NSC-derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were ... bone marrow stem cells in ischemic brain Acta Neurochir Suppl 2006, 99:123-124 Li J, Sun CR, Zhang H, Tsang KS, Li JH, Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells results ... seven of primary neural stem cells from cortices of E16 Sprague-Dawley rat embryos in three separate experiments A preponderance of NGF in pg per day of culture of × 106 cells was evident in culture...

Ngày tải lên: 18/06/2014, 16:20

... J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg DA: ... capacity of cells SVV enhanced the survival of Transplanted MSCs Cells were scattered in a number of colony distributions days after planted At day ~ 9, the bottle was covered with long-spindle cells ... Although so few cells with the neurons specific surface marker are detected, there is no electrophysiology or other evidences which can prove that these cells have the functions of the nerve cells Furthermore,...

Ngày tải lên: 18/06/2014, 19:20