2d dige observations of het hepatic mitochondrial proteome 86

... analysis of hepatoma cell response to butyrate treatment The immense advantages of proteomics offered by 2DE have not been utilized in this field In this study, we employed 2D-DIGE to uncover proteome ... suggested that up-regulation of GSN by butyrate is crucial for butyrate-mediated suppression of metastasis Trang 8INDEX OF TABLES Table 3.1: Labeling strategy for 2D-DIGE of heparin-bound and unbound ... eluted at 0.8 M NaCl 71 Figure 4.5: Representative images of 2D-DIGE analysis of (A) unbound and (B) heparin-bound protein fractions 120 µg of proteins from labeled internal standard, control and...

Ngày tải lên: 15/09/2015, 22:42

... properties of cytosolic and mitochondrial folate metabolism by experimenting with a mathematical model of hepatic one-carbon metabolism. The model is based on known biochemical properties of mitochondrial ... mitochondria and therefore the rate of export of formate to the cytosol declines to about 50% of its former value. Because of the reduced supply of formate, the concentration of cytosolic 10f-THF goes ... Disruption of the Mthfd1 gene reveals a monofunctional 10formyltetrahydrofolate synthetase in mammalian mitochondria J Biol Chem 2005, 280:7597-7602 Walkup... Cloning of human cDNAs encoding mitochondrial...

Ngày tải lên: 13/08/2014, 16:21

... the mitochondrial TK showed activity for taurocyamine, lombricine (30% of that of taurocyam- ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzed the phosphorylation of creatine. ... cluster of the CK portion is shown schematically by the representatives of three isoforms; cytoplasmic, mitochondrial and flagellar (a total of 71 CK sequences, available on the database of DDBJ, ... relation- ships, the structural correlations of this relaxed sub- strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified two cDNAs coding for...

Ngày tải lên: 07/03/2014, 21:20

... interacting with other PCAOB offices and divisions, such as the Office of Research and Analysis ("ORA") and the Office of the Chief Auditor ("OCA"), and using the results of tools available through ... valuation of deferred tax assets, valuation of goodwill, valuation of other intangibles and other long-lived assets, valuation of inventory, determination of otherthan-temporary impairment of certain ... not a result of an adversarial adjudicative process and not constitute conclusive findings of fact or of violations for purposes of imposing legal liability For additional discussion of this distinction,...

Ngày tải lên: 23/03/2014, 02:20

... Department of Biochemistry, University of Oxford, UK 3 School of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, UK The principal physiological role of mitochondrial ... eugleno-zoan mitochondrial cytochromes c [9,10] The reason for the loss of one thioether bond from the mitochondrial cytochromes c of euglenozoans is a longstanding puzzle It could be a means of altering ... group of Ala25 in C fasciculata cytochrome c (the equivalent residue of the first cysteine of the CXXCH motif of S cerevisiae cytochrome c) overlays almost perfectly with the CH2 group of the...

Ngày tải lên: 23/03/2014, 04:21

... multistep process of tumor metastasis Invasion of malignant tumor cells requires destruction of basement membranes and proteolysis of extracellular matrix (ECM) Of the several families of ECM-degrading ... Some inhibi-tors of the HGF/c-Met signaling pathway have been Figure 9 Volumes of hepatic VX2 carcinoma 21 days after reinoculation A, Photographs of the hepatic VX2 carcinoma of five rabbits ... doi:10.1186/1479-5876-8-73 Cite this article as: Ke et al.: Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma Journal of...

Ngày tải lên: 18/06/2014, 16:20

... disparities between the evolution rates of ssDNA and dsDNA viruses, proof of this may ultimately require a detailed comparative analysis of the individual impacts of all mutagenic reactions and repair ... undeniably important role of recombination in the generation of novel species and strains [20-25], it is the accumulation of point mutations that is the ultimate source of diversity within the ... biases suggest a possible cause of high MSV mutation rates Because of our relatively scant understanding of plant DNA replication in general, and more specifically of the host factors involved in...

Ngày tải lên: 20/06/2014, 01:20

... also a potent stimulator of neutrophils and is involved in the recruitment of leukocytes to the site of inflammation through the regulation of adhesion and the extravasion of neutrophils [45,48] ... relevant steps of lung fibrogenesis SSc-associated lung Figure 2 Hypothetical role of some disregulated BALf proteins in mechanisms driving SSc-related lung fibrosis Hypothetical role of some disregulated ... presence of serum anti-topoisomerase I (anti-Scl70) antibodies, but only 15% of patients eventually reach severe end-stage lung fibrosis [8] Given the complexity of the pathogenesis of lung fibrosis...

Ngày tải lên: 09/08/2014, 08:22

... Initial peptide populations Isoform 1 Isoform 2 Isoform 1 Isoform 2 Gene structures Exon skipping B A C Figure 1 Isoform and non-isoform specific peptides (A) Two protein isoforms (1 and 2) from an ... number of co-expressed protein isoforms in a given sample, but also on the Table 1 Identification of nuclear encoded TAIR 10 loci Set Total number of peptidesa Nr of mapped peptides % of peptides ... (Isoform 1: p1, p2, p4; Isoform 2: p1, p3, p4) when digested While peptides p1 and p4 are non-specific because they map to both isoforms, peptides p2 and p3 are specific for isoform 1 and isoform...

Ngày tải lên: 11/08/2014, 11:20

... levels by DIGE, silverstaining of DIGE gel, and immunoblotting 83 Figure 19 Immunoblotting of thioredoxin-2 and aconitase-2 85 Figure 20 2D- DIGE observations of HET hepatic mitochondrial ... disadvantages of major proteomic platforms 38 Table Experimental design of vehicle and drug-treatments of HET mice 50 Table Gel setup for 2D- DIGE experiments for HET hepatic mitochondrial proteome ... 56 Table Gel setup for 2D- DIGE experiments for analysis of troglitazone-induced changes in HET hepatic mitochondrial proteome 56 Table Hepatotoxicity score of HET mice treated with troglitazone...

Ngày tải lên: 12/09/2015, 11:08

... rate of plasma triglyceride input (intestinal and hepatic) and subtracted the rate of hepatic triglyceride secretion Briefly, to determine the total rate of triglyceride input in the plasma of mice, ... Rate of hepatic triglyceride secretion and intestinal triglyceride absorption in apoE) ⁄ ) and C57BL ⁄ mice One mechanism that could affect the hepatic triglyceride content is the secretion of hepatic ... (min) Fig Analysis of kinetic parameters associated with hepatic triglyceride content (A) Rate of hepatic very low-density lipoprotein (VLDL) triglyceride secretion (B) Rate of total triglyceride...

Ngày tải lên: 05/03/2014, 23:20

... effects of GH on the development of AFLD in the presence of alcohol are complex Both alcohol and GH exert a myriad of effects in vivo, and it is unclear whether the protective effects of GH against ... an important regulator of intrahepatic lipid metabolism Hepatic GH can interact with its receptor (GHR) on the surface of target cells and induces the association of GHR with Janus kinase (JAK)-2 ... percentage (VF%) of total body weight (VF weight/body weight × 100%) The hepatic index (HI) was calculated as liver weight/body weight × 100% Hepatic histology and measurement of triglyceride...

Ngày tải lên: 18/06/2014, 16:20

... and liver DNA of mice was observed The accumulation of titanium is consistent with the coefficients of liver and the liver injury of mice In addition, the accumulation of titanium of the organs ... decrease of ratio of ALB to GLB, TBIL levels which are important indicators of the hepatic injury, demonstrated that nano-anatase TiO2 induced hepatic injury Furthermore, the increase of enzyme activity ... histopathological changes of liver and the damage of hepatocyte substructure and with our previous report [12] Wang et al [8] showed that after a single oral gavage of dose of g/kg BW of TiO2 suspensions...

Ngày tải lên: 22/06/2014, 00:20

... of activated myofibroblasts) Secretion of type I collagen by myofibroblasts that leads to fibrosis was analyzed by immunofluorescence The results of imunofluorescence study for distribution of ... enhanced the process of differentiation Wang et al (2003) showed that hepatic differentiation of HSC could Page of 11 be enhanced by intravenous injection of soluble HGF None of the earlier reports ... distribution and stability of HGFCNP in the blood circulation Whether HGF released from the CNP causes the stem cell differentiation or apoptosis of myofibroflasts or both of these functions must...

Ngày tải lên: 11/08/2014, 00:23

... results indicate that mitochondrial translocation of Bax may constitute a direct cause of cytochrome c release ROS has also been demonstrated to induce the depolarization of the mitochondrial membrane, ... 500 lL of NaCl ⁄ Pi and 10 mL of 70% ethanol at °C overnight; then, after washing twice with NaCl ⁄ Pi, the cells were incubated with mL of PI staining solution (50 mgÆL)1 of PI and gÆL)1 of RNase ... NAC suppresses oridonin-induced mitochondrial membrane potential (MMP) collapse and apoptosis Subsequently, we examined the integrity of the mitochondrial membranes of cells by rhodamine 123 staining...

Ngày tải lên: 18/02/2014, 13:20

... quantities below the detection limit of XRD As a general rule, the detection limit of the XRD analyses of crystalline phases is considered to be 5% of the total weight of the mixture However, several ... where a large fraction of As (III) occurs as HAsO2y, the precip3 itation of CaHAsO3 will take place Within the same pH range, the formation of Ca3(AsO4)2 occurs in the presence of As(V) ions These ... include: degree of crystallinity, hydration, surface texture of the sample, sample weight, particle orientation, mass absorption coefficients of different minerals, etc (Mitchell, 1993) Any of these...

Ngày tải lên: 15/03/2014, 23:48

... Characterization of the purified soluble proteins The identity of the prepared protein was confirmed by sequence mapping of tryptic digests with MALDI-TOF MS with a good sequence coverage The total mass of the ... Instruments, Malvern, UK) Estimates of hydrodynamic radii of the expected molecular species were calculated with the software hydropro [26] The structures of monomers and dimers of the human CD69 in the ... asymmetric unit of the crystal structure of CD69 (the asymmetric unit comprises one dimer of CD69 and three Cl) anions) Every monomer binds two Cl– anions, one in a shallow pocket at the side of the molecule...

Ngày tải lên: 16/03/2014, 04:20

... serum levels of laminin (a component of intracellular matrix) were found to be predictive of any form of fibrosis in 30 patients with NAFLD.[36] The measurement of serum levels of other fibrogenic ... protective effects of estrogen In children the majority of patients are diagnosed during the second decade of life The exact prevalence of NAFLD in children is unknown The prevalence of fatty liver ... 247 World Journal of Pediatrics serum levels of aminoterminal propeptide of type III collagen, hyaluronic acid and tissue inhibitor of matrix metalloproteinase I in predicting hepatic fibrosis...

Ngày tải lên: 22/03/2014, 10:20

... not treated well Signs of CLD AIH • AIH is a hepatic inflammation associated with the presence of circulating autoantibodies in the absence of other recognized causes of liver disease • Associated ... Presentation of cholestasis • Jaundice (accumulation of conjugated bilirubin) • • • • Pale stool (Acholic stool)?? Dark and foamy urine (bile salts in the urine) Pruritis (accumulation of bile salts) ... (accumulation of cholestrol) • Hepatomegaly +/- Splenomegaly • Failure to thrive (FTT)- neonates • Incidental lab finding Signs of cholestatic liver disease Hepato-cellular disease • Necrosis of hepatocytes...

Ngày tải lên: 28/03/2014, 09:20

... investigations involved determination of IMPDH activity, analyses of IMPDH and expression and characterization of T cell subpopulations The PK and PD profiles of MPA changed with time after transplantation ... range of total observations Profiles of patients in the belatacept group (n = 3) at weeks 1, and 13 (A, B and C) and the cyclosporine group (n = 3) at weeks 1, and 13 (D, E and F) Page 10 of 14 ... The postdose activities of IMPDH were strongly influenced by MPA exposure At week 1, the activity profiles for of the patients were inversely related to MPA concentraPage of 14 (page number not...

Ngày tải lên: 18/06/2014, 15:20