fi ling of 2 ab labeled n glycans

... functional blocking antibody to b1 These findings may explain why bisecting GlcNAc-containing N- glycans are abundant in the brain [65] In fact, mice carrying an inactive GnT-III mutant have an ... dependent on branched N- glycans, and an important aspect of this dependence is galectin binding It is worth noting that fibronectin polymerization and tumor cell motility are regulated by binding ... of T lymphocytes and epithelial cells to show that galectin binding to N- glycans on membrane glycoproteins enhances surface residency, and is dependent on N- glycan number (protein encoded) and...

Ngày tải lên: 18/02/2014, 17:20

... of neutrophil proteinases by recombinant ela n FEBS Journal 27 5 (20 08) 20 08 20 20 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 20 19 Antibacterial and antifungal activities of trappin -2 22 ... on the antimicrobial 20 12 Fig Western blot analysis of ela n and trappin -2 fractionated on heparin-Sepharose The heparin-binding capacities of ela n and trappin -2 were evaluated by affinity chromatography ... Trappin -2 20 Trappin -2 + heparin Trappin -2 + NaCl 0 10 15 20 25 Polypeptide (µM) 30 35 C albicans 100 % of surviving CFU 90 80 70 60 Trappin -2 Trappin -2 + heparin 50 Trappin -2 + NaCl 40 10 15 20 25 ...

Ngày tải lên: 18/02/2014, 17:20

... terminal structures of the N- glycans of GAT1, N- glycosylation processing of NNN was inhibited by 1-deoxymannojirimycin (dMM) Inhibition by dMM leads to the formation of NNN molecules containing N- glycans ... stability of NNN containing only mannosidic N- glycans after treatment with dMM 1 629 Role of N- glycosylation and N- glycan trimming of GAT1 G Cai et al Fig Analysis of the cell surface expression ... influence of the N- glycosylation and N- linked oligosaccharides on protein stability, the FEBS Journal 27 2 (20 05) 1 625 –1638 ª 20 05 FEBS Fig Influence of dMM on plasma membrane trafficking and GABA-uptake...

Ngày tải lên: 19/02/2014, 17:20

... DCSIGN, DC-SIGNR and LSECtin exhibited common binding affinity to mono- and ⁄ or bi-antennary agalactosylated N- glycans Although affinity enhancement by multivalent lectin–carbohydrate interaction ... complex-type N- glycans were significantly lower than those for high-mannose-type N- glycans (005-017, > 29 2 lm), unlike the case of DC-SIGN Addition of Gal on either GlcNAc residue of 20 2 or 103 abolished ... protein in a Ca2+-dependent manner [28 ,29 ] Subsequently, Powlesland et al [30] suggested the presence of bi-antennary agalactosylated N- glycans, as well as high-mannose-type N- glycans, on Ebola...

Ngày tải lên: 15/03/2014, 23:20

... Metabolic activation and detoxication of arylamines Curr Med Chem 3, 19 521 0 Hein DW (20 02) Molecular genetics and function of NAT1 and NAT2: role in aromatic amine metabolism and carcinogenesis Mutat ... chain of Tyr190, the side chain of Asn 72 and the backbone of Gly 124 and Ala 123 participate in a network of interactions with Asp 122 Moreover, because the centroid of the Tyr190 phenyl ring is ... Arylamine N- acetyltransferases: covalent modication and inactivation of hamster NAT1 by bromoacetamido derivatives of aniline and 2- aminouorene J Protein Chem 22 , 6316 42 24 Dupret JM & Grant DM (19 92) ...

Ngày tải lên: 16/03/2014, 00:20

... fucosylation, LDNF- LDNF- antenna, LDN- antenna core-(a1–6)- fucosylation, LDNF- LDNF- antenna, LDNF- antenna LDN-LDN- antenna, LDN- antenna, antenna of a single HexNAc residue (truncated) core-(a1–6)- ... core-(a1–6)-fucosylation, LDNF-LDNFantennae, antenna of a single HexNAc residue (truncated) – core-(a1–6)-fucosylation, LDN-LDN- antenna, LDNF-LDN- antenna, LDN- antenna core-(a1–6)-fucosylation, LDNF-LDN antenna, ... fucosylation, LDNF-LDN- antenna, antenna of a single HexNAc residue (truncated) core-(a1–6)-fucosylation, LDNF-LDNF- antenna, antenna of a single HexNAc residue (truncated) LDN-LDN antennae, possibly...

Ngày tải lên: 16/03/2014, 14:20

... GlcNAc I in an early processing stage of the developing N- glycan as it has been shown previously for insects and nematodes [27 ,28 ] The paucimannosidic glycans eluting in the first two regions on reversed ... Biochem 23 2, 27 2 28 3 Mulder, H., Spronk, B.A., Schachter, H., Neeleman, A.P., Van den Eijneden, D.H., De Jong-Brink, M., Kamerling, J.P & Vliegenthart, J.F.G (1995) Identification of a novel UDP-GalNAc:GlcNAcb-Rb1-4 ... paucimannose N- glycans by Caenorhabditis elegans requires prior actions of UDP -N- acetylD-glucosamine:a-3-D-mannosideb1 ,2- N- acetylglucosaminyl transferase I, a3,6-mannosidase II and a specific membrane-bound...

Ngày tải lên: 16/03/2014, 16:20

... HexNAc2Hex11 HexNAc2Hex10 HexNAc2Hex6 HexNAc2Hex5 HexNAc3Hex3 HexNAc2Hex4 HexNAc2Hex3 HexNAc3Hex5PC1 HexNAc3Hex3Fuc2 HexNAc4Hex3 HexNAc2Hex3Fuc2 HexNAc2Hex2Fuc2 HexNAc2Hex2Fuc1 HexNAc2Hex2 HexNAc4Hex5Fuc1 ... HexNAc2Hex6 1475.5485 [M +2H ]2+ HexNAc2Hex7 1637.6014 [M +2H ]2+ HexNAc2Hex8 1799.6541 [M +2H ]2+ HexNAc2Hex9 1961.7070 [M +2H ]2+ 21 23.7597 [M +2H ]2+ HexNAc2Hex10 HexNAc2Hex11 22 85.8 125 [M +2H ]2+ ... In the second group, PC-containing protein-linked N- glycans have been found in C elegans [19 22 ], Ac viteae [23 ], T spiralis [24 ] and O volvulus [25 ] These N- glycans contain the typical trimannosyl...

Ngày tải lên: 23/03/2014, 09:21

... signals correspond to high-mannose glycans containing from to mannose residues (GlcNAc2Man3 to GlcNAc2Man9) (Table 1) Interestingly, signals at m ⁄ zvalues 20 29.5 (20 29.9), 20 69.1 (20 69.3) and 23 95.4 ... 20 28.71 20 29.73 20 29.5 20 29.9 HexNAc5Man3Gal3 20 69.74 20 70.78 20 69.1 20 69.3 HexNAc6Man3Gal2 23 93.85 23 95.06 23 95.4 23 95.1 HexNAc6Man3Gal4 Structure HexNAc2Man4 HexNAc2Man5 a Upper number indicates the ... (A) and (E), analysis under ‘conditions a’ for neutral glycan; (B), (C) and (D), analysis under ‘conditions b’ for acidic glycans Standards: 1, Man3GlcNAc2OH; 2, Man5GlcNAc2OH; 3, Man6GlcNAc2OH;...

Ngày tải lên: 23/03/2014, 15:20

... [2, 3, 5,11, 12] However, patients’ sera containing antiglycan immunoglobulin can bind to a variety of plant and insect glycoproteins and even to human proteins unrelated to any allergen in the peptide ... common hyaluronan binding motif in the hyaluronan binding proteins RHAMM, CD44 and link protein Embo J 13, 28 6 29 6 17 Wypych JI, Abeyounis CJ & Reisman RE (1989) Analysis of differing patterns of ... hyaluronidases contain the difucosylated paucimannosidic N- glycans MUF3F6 and MMF3F6 (Fig 3) as the major species In addition, glycans containing one or two GlcNAc residues at the nonreducing end and...

Ngày tải lên: 23/03/2014, 15:20

... carbohydrate-dependent manner Mixtures of lactose and (asialo)fetuin were expected to be potent inhibitors Carbohydrate-independent binding of the neoglycoprotein and biotin-independent binding of the second-step ... Restrained vs free dynamics simulations of oligosaccharides: application to solution 21 22 23 24 25 26 27 dynamics of biantennary and bisected biantennary N- linked glycans Biochemistry 33, 9606–9614 ... [37] N- glycans [37] in Table The general conclusion is that the presence of a bisecting GlcNAc affects lectin affinity In the case of mistletoe lectin, the introduction of a bisecting GlcNAc into...

Ngày tải lên: 30/03/2014, 13:20

... a-Manb a-Fucc a-Manb a-Fucc b-GlcNAcd H2-1 F1-5 F2-1 F2 -2 F3-1 F4-1 F4 -2 F4-3 F4-4 F5-1 F5 -2 Man5GlcNAcPA Gal2Man3GlcNAc3PA Man2GlcNAc2PA Gal1Man2GlcNAc2PA Man6GlcNAc2PA Man2GlcNAc2FucPA Man3GlcNAc2PA ... Na]+ Na]+ H]+ H]+ H]+ Na]+ H]+ Na]+ H]+ Na]+ H]+ Na]+ Na]+ H]+ Na]+ H]+ H]+ Na]+ Na]+ H]+ H]+ Na]+ Na]+ Na]+ H]+ Na]+ Na]+ Na]+ Na]+ Na]+ Na]+ H + Na ]2+ H + Na ]2+ H + Na ]2+ H + Na ]2+ H + Na ]2+ ... Hex2HexNAc2dHexPA Hex3HexNAc2PA Hex5HexNAc Hex5HexNAc Hex4HexNAc2 Hex4HexNAc2PA Hex6HexNAc Hex5HexNAc2 Hex5HexNAc2PA Hex4HexNAc3PA HexHexNAc2dHexPA Hex2HexNAc2PA Hex2HexNAc2dHexPA Hex3HexNAc2PA...

Ngày tải lên: 31/03/2014, 08:20

... C-terminally of a single arginine residue or at a multibasic recognition motif by the subtilase furin [11] N GP -2 N GP-C 22 6 23 4 22 4 7 - 22 4 21 6 21 7 24 3 11 316 317 - aa length GP-C 367 375 3 92 397 ... antigenicity and activity [ 12- 21] Glycoproteins of arenaviruses vary considerably in number and position of potential N- glycosylation sites as shown by an N- glycosylation prediction program (Table ... Importance of hemagglutinin glycosylation for the biological functions of influenza virus Virus Res 20 02, 82( 1 -2) :73-75 24 25 Koch M, Pancera M, Kwong PD, Kolchinsky P, Grundner C, Wang L, Hendrickson...

Ngày tải lên: 20/06/2014, 01:20

... Picture Financial Planning Is Not Just Forecasting Three Requirements for Effective Planning Financial Planning Models Components of a Financial Planning Model An Example of a Planning Model An Improved ... the effect of growth on the need for external financing What Is Financial Planning? Financial planning is a process consisting of: Analyzing the investment and financing choices open to the firm ... the end result is called a financial plan New investments need to be paid for So investment and financing decisions cannot be made independently Financial planning forces managers to think systematically...

Ngày tải lên: 05/08/2014, 13:20

... landlord-tenant law and provides links to federal and state statutes http://tenant.net TenantNet provides information about landlord-tenant law, with a focus on tenants’ rights TenantNet is designed ... become profit- l sylvania Avenue, NW, Washington able The document usually starts with DC 20 850, 20 2- 326 -22 22, http:// a statement outlining the purpose and l www.ftc.gov, and by contacting your ... Department of Labor office You should be able to find a listing under U.S Government, Department of Labor, in the phone book You can also find a list of local offices of the U.S Department of Labor...

Ngày tải lên: 07/08/2014, 04:20

... of the morn and goddess of the evening' One of the most prominent figures in the Assyro-Babylonian pantheon, Ishtar was the divine personification of the planet Venus While the Assyro-Babylonians ... queen, who then bore a son who, in his turn, mounted the throne of the living In temples, and particularly those of Nubia, many ancient kings and the living king himself were often worshipped in ... in honour of Anu and Ishtar, and at Ur in honor of Nannar Asshur But the day came when the might of Babylon faded before that of Nineveh The national god of the Assyrians, Asshur, then took the...

Ngày tải lên: 07/08/2014, 04:20

... extension of ≤ on Tn Moreover, these two orders C D coincide for n ≤ and ≤ is a proper extension of ≤ for n ≥ 6, as shown in [M] There is a significant simplification when one considers only tableaux ... is an G extension of ≤ C G G D Consequently, ≤ is an extension of ≤ and ≤ is an extension of ≤ All three orders C G coincide for n ≤ 5, and ≤ is a proper extension of ≤ which is, in turn, a ... closures of nilpotent order in sln 1. 12 The body of the paper consists of two sections In section we explain all the background in combinatorics of Young tableaux essential in the subsequent analysis...

Ngày tải lên: 07/08/2014, 08:22

... dynamic system composed of person and environment, as well as the phenomenology of person-environment interactions Rock climbers, surgeons, and others who routinely find deep enjoyment in an activity ... despite considerable variation in operational definitions of resilience, findings from a diverse literature point to the same conclusions with compelling consistency Given the considerable degree of debate ... place in consciousness, such as thinking, willing, and feeling about this information (i.e., cognition, motivation, and emotion) The memory system then stores and retrieves the information We can...

Ngày tải lên: 08/08/2014, 00:22

... theory of communication’ appear It won instant acceptance Shannon had invented his own branch of science and was treading on nobody else’s toes His propositions, though wholly new and surprising, ... contradictory and often erroneous But a new fashion was beginning in the 1960s, for drilling into the ice sheets of Greenland and Antarctica, and recovering in long cores the ice formed from snowfall ... turning point in brain research came in the 1960s when David Ingvar in Lund and Niels Lassen in Copenhagen began introducing into the bloodstream a radioactive material, xenon-133 The scientists...

Ngày tải lên: 08/08/2014, 01:20

... obtained and the selectivity was good H HO N R2 OR1 R N N R = H, cinchonidine R = OMe, quinine N H R1 = R2 = H, cinchonine R1 = CH3CO, R2 = H R1 = PhCO, R2 = H R1 = H, R2 = OMe, quinidine Figure ... under neat conditions.14 Thermal conditions were used and there was no investigation of applying a catalyst to conduct the reaction using milder conditions With many of the parameters remaining ... reaction of 2( 1H)-pyridones with N- phenyl maleimide under neat conditions A recent report by Fujita and co-workers made use of 2( 1H)-pyridones as dienes for the DA reactions with N- phenyl maleimide...

Ngày tải lên: 14/09/2015, 08:47