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94 - 2001 Sampling to Detect Rare Diseases By Preben Willeberg Danish Slaughterhouses, Axelborg, Axeltorv 3, DK-1609 Copenhagen V, Denmark.. Monitoring / surveillance and estimation / de

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Acta vet scand 2001, Suppl 94, 93-93.

Acta vet scand Suppl 94 - 2001

Sampling to Detect Rare Diseases

By Preben Willeberg

Danish Slaughterhouses, Axelborg, Axeltorv 3, DK-1609 Copenhagen V, Denmark.

Monitoring / surveillance and estimation /

de-tection of specific diseases are common

proce-dures used in national disease control in farmed

animals When the disease is assumed to be rare

or even absent, such activities are especially

im-portant, e.g in documenting a preferable

zoo-sanitary status as part of trade agreements The

statistical and epidemiological aspects of

de-signing such investigations and the difficulties

in properly analysing and interpreting the data

are far from trivial This presentation will point

to some of these problems and it will suggest

ways and means of minimising some of the

dif-ficulties

Screening for disease to estimate its prevalence,

e.g during the course of a control or eradication

program, must be based on a valid sampling

scheme Examples of such will be given from

recent field studies

Documenting freedom from disease must

simi-larly be based on sampling when the aim is to

document a continued freedom from a disease

never found in, or previously eradicated from,

the population It is important to realise,

how-ever, that proving initial absolute freedom from

a new or exotic disease cannot be made from a representative sample of the “normal” popula-tion! Examples of the confusion created by not recognising this distinction will be given Another common problem in designing and in-terpreting farm animal disease sampling is the frequently neglected effect of clustering by herd and other units, which will be illustrated and discussed

The validity of the diagnostic procedures used

to characterise the individuals in the sample as either positive or negative are of similar impor-tance Estimation and optimisation of the test sensitivity and specificity are integral parts of performing a survey or maintaining a surveil-lance programme

Various methods to improve test sensitivity and specificity will be mentioned, and the impor-tance of being able to estimate these character-istics will be illustrated Newer methods for these purposes have been introduced in the field, and these will be discussed

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