Handbook of Microbiological Media, Fourth Edition part 101 docx

2.0mg Cyanocobalamin ...100.0μg Preparation of Wolfe’s Vitamin Solution : Add components to distilled/deionized water and bring volume to 1.0L.. 0.19g Preparation of Ferric Quinate Solut

Trang 1

Magnetospirillum Medium 995

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of Yersinia enterocolitica from foods.

Magnetic Spirillum Growth Medium, Revised

(MSGM, Revised)

Compositionper liter:

Agar 1.3g

KH2PO4 0.68g

Tartaric acid 0.37g

Succinic acid 0.37g

NaNO3 0.12g

Sodium acetate 0.05g

Ascorbic acid 0.035g

Wolfe’s vitamin solution 10.0mL

Wolfe’s mineral solution 5.0mL

Ferric quinate solution 2.0mL

Resazurin (0.1% solution) 0.45mL

pH 6.75 ± 0.2 at 25°C

Wolfe’s Vitamin Solution:

Composition per liter:

Pyridoxine·HCl 10.0mg

Thiamine·HCl 5.0mg

Riboflavin 5.0mg

Nicotinic acid 5.0mg

Calcium pantothenate 5.0mg

p-Aminobenzoic acid 5.0mg

Thioctic acid 5.0mg

Biotin 2.0mg

Folic acid 2.0mg

Cyanocobalamin 100.0μg

Preparation of Wolfe’s Vitamin Solution : Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Wolfe’s Mineral Solution:

MgSO4·7H2O 3.0g

Nitriloacetic acid 1.5g

NaCl 1.0g

MnSO4·H2O 0.5g

FeSO4·7H2O 0.1g

CoCl2·6H2O 0.1g

CaCl2 0.1g

ZnSO4·7H2O 0.1g

CuSO4·5H2O 0.01g

AlK(SO4)2·12H2O 0.01g

H3BO3 0.01g

Na2MoO4·2H2O 0.01g

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water Dissolve by adjusting pH

to 6.5 with KOH Add remaining components Add distilled/deionized

water to 1.0L

Ferric Quinate Solution:

Compositionper 100.0mL:

FeCl3 0.27g

Quinic acid 0.19g

Preparation of Ferric Quinate Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C

Preparation of Medium: To 1.0L of distilled/deionized water add components in the following order: Wolfe’s vitamin solution, Wolfe’s min-eral solution, ferric quinate solution, resazurin, KH2PO4, NaNO3, ascorbic acid, tartaric acid, succinic acid, sodium acetate, and agar Mix thoroughly after each addition Adjust pH to 6.75 with NaOH Autoclave for 15 min

at 15 psi pressure–121°C Aseptically distribute into sterile screw-capped tubes Fill tubes to capacity with medium Use a heavy inoculum in each tube and do not introduce a headspace of air Screw down caps tightly

Use: For the cultivation and maintenance of Aquaspirillum magnetot-acticum.

Magnetospirillum Medium

(DSMZ Medium 380)

KH2PO4 0.68g

L(+)-Tartaric acid 0.37g Succinic acid 0.37g NaNO3 0.12g Na-thioglycolate 0.05g Na-acetate 0.05g Resazurin 0.5mg Vitamin solution 10.0mL Trace elements solution 5.0mL Ferric quinate solution 2.0mL

pH 6.8 ± 0.2 at 25°C

Trace Elements Solution:

MgSO4·7H2O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO4·2H2O 0.5g CoSO4·7H2O 0.18g ZnSO4·7H2O 0.18g CaCl2·2H2O 0.1g FeSO4·7H2O 0.1g NiCl2·6H2O 0.025g KAl(SO4)2·12H2O 0.02g

H3BO3 0.01g

Na2MoO4·4H2O 0.01g CuSO4·5H2O 0.01g

Na2SeO3·5H2O 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water Dissolve by adjusting pH

to 6.5 with KOH Add remaining components Add distilled/deionized water to 1.0L Mix thoroughly

Vitamin Solution:

Pyridoxine-HCl 10.0mg Thiamine-HCl·2H2O 5.0mg Riboflavin 5.0mg Nicotinic acid 5.0mg

D-Ca-pantothenate 5.0mg

Lipoic acid 5.0mg Biotin 2.0mg Folic acid 2.0mg Vitamin B12 0.1mg

Trang 2

996 Magnetospirillum 2 Medium

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L Mix thoroughly Sparge

with 80% H2 + 20% CO2 Filter sterilize

Composition per 100.0mL:

FeCl3·6H2O 0.45g

Quinic acid 0.19g

Preparation of Ferric Quinate Solution: Add components to

distilled/deionized water and bring volume to 100.0mL Sparge with

N2 Autoclave for 15 min at 15 psi pressure–121°C Cool to 25°C

Preparation of Medium: Add components, except vitamin

solu-tion and ferric quinate solusolu-tion, to distilled/deionized water and bring

volume to 988.0mL Purge medium with N2 gas for 10 min Mix

thor-oughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 25°C

Aseptically and anerobically add 10.0mL vitamin solution and 2.0mL

ferric quinate solution Mix thoroughly Purge medium with N2 gas for

10 min Under the same atmosphere, anaerobically fill tubes to 1/3 of

their volume and seal Autoclave at 121°C for 15 min Before

inocula-tion, add sterile air (with hypodermic syringe through the rubber

clo-sure) to 1% O2 concentration in the gas phase

Use: For the cultivation of Magnetospirillum

magnetotacti-cum=Aquaspirillum magnetotacticum, and Magnetospirillum

gryph-iswaldense.

Magnetospirillum 2 Medium

Sodium acetate 1.0g

K2HPO4 0.5g

Sodium thioglycolate 0.5g

NH4Cl 0.1g

Yeast extract 0.1g

Ferric citrate 20.0μg

pH 6.8 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Distribute into

screw-capped tubes in 5.0mL volumes Autoclave for 15 min at 15 psi

pres-sure–121°C Allow medium to stand upright at room temperature for

2 to 3 days before inoculation Do not shake

Use: For the cultivation and maintenance of Magnetospirillum

gryph-iswaldense.

Magnetospirillum Semi-solid Medium

(DSMZ Medium 380)

Agar 1.3g

KH2PO4 0.68g

L(+)-Tartaric acid 0.37g

Succinic acid 0.37g

NaNO3 0.12g

Na-thioglycolate 0.05g

Na-acetate 0.05g

Resazurin 0.5mg

Vitamin solution 10.0mL

Trace elements solution 5.0mL

Ferric quinate solution 2.0mL

pH 6.8 ± 0.2 at 25°C

Trace Elements Solution:

MgSO4·7H2O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO4·2H2O 0.5g CoSO4·7H2O 0.18g ZnSO4·7H2O 0.18g CaCl2·2H2O 0.1g FeSO4·7H2O 0.1g NiCl2·6H2O 0.025g KAl(SO4)2·12H2O 0.02g

H3BO3 0.01g

Na2MoO4·4H2O 0.01g CuSO4·5H2O 0.01g

Na2SeO3·5H2O 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water Dissolve by adjusting pH

to 6.5 with KOH Add remaining components Add distilled/deionized water to 1.0L Mix thoroughly

Vitamin Solution:

Pyridoxine-HCl 10.0mg Thiamine-HCl·2H2O 5.0mg Riboflavin 5.0mg Nicotinic acid 5.0mg

D-Ca-pantothenate 5.0mg

Lipoic acid 5.0mg Biotin 2.0mg Folic acid 2.0mg Vitamin B12 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L Mix thoroughly Sparge with 80% H2 + 20% CO2 Filter sterilize

Composition per 100.0mL:

FeCl3·6H2O 0.45g Quinic acid 0.19g

Preparation of Ferric Quinate Solution: Add components to distilled/deionized water and bring volume to 100.0mL Sparge with

N2 Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°C

Preparation of Medium: Add components, except vitamin solu-tion and ferric quinate solusolu-tion, to distilled/deionized water and bring volume to 988.0mL Purge medium with N2 gas for 10 min Mix thor-oughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°C Aseptically and anerobically add 10.0mL vitamin solution and 2.0mL ferric quinate solution Mix thoroughly Purge medium with N2 gas for

10 min Dispense 12mL of medium per 16 x 150mm anaerobe screw-cap tube under N2 gas Prior to inoculation, remove caps briefly under air, tighten the caps again, and wait several hours to establish oxygen gradients The medium should be slightly pink in color Strongly re-duced conditions will not support growth of the organism During growth O2 will be consumed, resazurin decolorized, and the pH in-creased Feed oxygen (by adding air) and succinic acid from sterile

0.05M solution (to maintain pH below 7.0) If higher densities of

mag-netic cell are wanted, ferric quinate also can be fed For transfer use cell material which has been concentrated at the glass wall of the culture vessel by means of a magnetic rod attached outside

Trang 3

Maleate Medium for Pseudomonas fluorescens with Glucose and Phenol Red 997

Use: For the cultivation of Magnetospirillum magnetotacticum

(Aquaspirillum magnetotacticum) and Magnetospirillum

gryphiswald-ense.

Maintenance HiVeg Medium for B subtilis ATCC

6633

Agar 15.0g

Plant peptone 6.0g

Plant hydrolysate 4.0g

Yeast extract 3.0g

Plant extract 1.5g

Glucose 1.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring until boiling Autoclave for 15 min at 15 psi pressure–121°C

Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of Bacillus subtilis.

Maintenance of L Antigen in Neisseria

Proteose peptone No 3 20.0g

Agar 15.0g

Na2HPO4 5.0g

NaCl 5.0g

Glucose 0.5g

Rabbit blood, defibrinated 100.0mL

pH 7.4–7.6 at 25°C

Preparation of Medium: Add components, except rabbit blood, to

distilled/deionized water and bring volume to 900.0mL Mix

thorough-ly Gently heat while stirring until boiling Autoclave for 20 min at 15

psi pressure–121°C Cool to 60°C Aseptically add 100.0mL of sterile,

defibrinated rabbit blood Maintain at 75°C while shaking for 30 min

Use: For the cultivation and maintenance of Neisseria gonorrhoeae.

Maintenance SCY Medium

See: SCY Medium

Maintenance (SCY) HiVeg Medium

Agar 10.0g

Sucrose 1.0g

Plant hydrolysate 0.91g

Yeast extract 0.25g

NaCl 0.05g

Papaic digest of soybean meal 0.03g

K2HPO4 0.02g

Thiamine 0.4mg

pH 7.3 ± 0.2 at 25°C

Hi-Media

water and bring volume to 1.0L Mix thoroughly Filter sterilize

Use: For the cultivation and maintenance of iron and sulfur bacteria

Malachite Green Broth

Peptone 15.0g Beef extract 9.0g Malachite Green 0.01mg

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Pseudomonas aeruginosa

Maleate Medium for Pseudomonas fluorescens

Agar 15.0g

K2HPO4 1.13g

NH4NO3 1.0g

KH2PO4 0.48g MgSO4·7H2O 0.2g Potassium maleate solution 8.61mL

pH 7.0 ± 0.2 at 25°C

Potassium Maleate Solution:

Maleic acid 116.07g

KOH (10N solution) 200.0mL

Preparation of Potassium Maleate Solution: Add maleic acid

to distilled/deionized water and bring volume to 600.0mL Slowly add KOH solution (generates heat) Bring volume to 1.0L with distilled/de-ionized water Adjust pH to 7.0 Filter sterilize

Preparation of Medium: Add components, except potassium maleate solution, to distilled/deionized water and bring volume to 991.4mL Mix thoroughly Gently heat while stirring until boiling Au-toclave for 20 min at 15 psi pressure–121°C Cool to 50°C Aseptically add 8.61mL of the potassium maleate solution Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Pseudomonas fluorescens and Mycoplasma pneumoniae.

Maleate Medium for Pseudomonas fluorescens

with Glucose and Phenol Red

Agar 15.0g Glucose 10.0g

K2HPO4 1.13g

NH4NO3 1.0g

KH2PO4 0.48g MgSO4·7H2O 0.2g Phenol Red 0.04g Potassium maleate solution 8.61mL

pH 7.0 ± 0.2 at 25°C

Potassium Maleate Solution:

Maleic acid 116.07g

KOH (10N solution) 200.0mL

Preparation of Potassium Maleate Solution: Add maleic acid

to distilled/deionized water and bring volume to 600.0mL Slowly add KOH solution (generates heat) Bring volume to 1.0L with distilled/de-ionized water Adjust pH to 7.0 Filter sterilize

Trang 4

998 Malonate Broth

Preparation of Medium: Add components, except potassium

maleate solution, to distilled/deionized water and bring volume to

991.4mL Mix thoroughly Gently heat while stirring until boiling

Au-toclave for 20 min at 15 psi pressure–121°C Cool to 50°C Aseptically

add 8.61mL of the potassium maleate solution Mix thoroughly Pour

into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Pseudomonas fluorescens

and Mycoplasma pneumoniae.

Malonate Broth

Sodium malonate 3.0g

NaCl 2.0g

(NH4)2SO4 2.0g

K2HPO4 0.6g

KH2PO4 0.4g

Bromthymol Blue 0.025g

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid

intro-duction of carbon and nitrogen from other sources

Use: For the cultivation and differentiation of coliforms and other

enteric organisms, particularly Enterobacter and Escherichia, based on

their ability to utilize malonate as the sole carbon source and

ammo-nium sulfate as the sole nitrogen source Malonate-utilizing organisms

turn the medium blue

Malonate Broth, Ewing Modified

Sodium malonate 3.0g

NaCl 2.0g

(NH4)2SO4 2.0g

Yeast extract 1.0g

Glucose 0.25g

K2HPO4 0.6g

KH2PO4 0.4g

Bromthymol Blue 0.025g

pH 6.7 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and differentiation of coliforms and other

enteric organisms, particularly Enterobacter and Escherichia, based on

their ability to utilize malonate as a carbon source and ammonium

sul-fate as a nitrogen source The small amount of yeast extract and

glu-cose encourages the growth of some organisms that may be distressed

or fail to respond Malonate-utilizing organisms turn the medium blue

Malt Agar

Malt extract 30.0g

Agar 15.0g

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring until boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–118°C Do not overheat or agar will not harden Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of yeasts and molds

Malt Agar

Agar 20.0g Malt extract 12.5g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat until boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of fungi

Malt Agar, 1/3 Strength (ATCC Medium 2365)

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Malt Agar, Blakeslee

Glucose 20.0g Malt extract 20.0g Agar 16.0g Mycological peptone 1.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Acremonium chrysogenum, Agaricus bisporus, Agrocybe aegerita, Armillaria mellea, Aspergillus spe-cies, Aureobasidium pullulans, Basidiomycetes spespe-cies, Candida spespe-cies, Ceratocystis adiposa, Citeromyces matritensis, Cladosporium cucumeri-num, Cochliobolus miyaheanus, Colletotrichum destructivum, Colletotri-chum lindemuthianum, Cryptococcus albidus, numerous other Cryptococ-cus species, Debaryomyces polymorphus, Diaporthe magnusii, Diaporth-ephaseolorum, Drechslera spicifera, Fusarium graminearum, Geotri-chum lactis, other GeotriGeotri-chum species, Hanseniaspora uvarum, Hanse-niaspora valbyensis, Hansenula subpelliculosa, Humicola species, Kloeckera apiculata, Kloeckera corticis, Kluyveromyces lodderi, Kluyver-omyces marxianus, Metschnikowia pulcherrima, Monilinia fructigena,

Trang 5

Malt Agar 4% with Lupine Stems 999

Myrothecium verrucaria, Myxozyma melibiosi, Nadsonia fulvescens,

Neu-rospora crassa, Octosporomyces octosporus, Pachysolen tannophilus,

Paecilomyces variotii, Penicillium aurantiogriseum, many other

Penicil-lium species, Pithoascus schumacheri, Rhizoctonia crocorum,

Rhizomu-cor miehei, Rhodosporidium toruloides, Rhodotorula glutinis,

Rhodoto-rula minuta, RhodotoRhodoto-rula rubra, Saccharomyces cerevisiae,

Saccharomy-ces exiguus, SaccharomySaccharomy-ces ludwigii, SaccharomySaccharomy-ces capsularis,

Saccha-romyces fibuligera, SchizosacchaSaccha-romyces pombe, Sporobolomyces

para-roseus, Sporobolomyces salmonicolor, Sporopachydermia lactativora,

Stachybotrys species, Talaromyces emersonii, Taphrina deformans,

Toru-laspora delbrueckii, Trichoderma harzianum, Trichoderma koningii,

Trichoderma longibrachiatum, Trichoderma reesei, Trichoderma viride,

Trichosporon beigelii, Wickerhamia fluorescens, Yarrowia lipolytica, and

Zygosaccharomyces veronae.

Malt Agar 1%

(MA1)

Agar 15.0g

Malt extract 10.0g

Use: For the cultivation of Acrodontium griseum, Acrodontium simplex,

Acrophialophora fusispora, Acrothecium tenebrosum, Acrothecium

capsic, Agaricus bisporus, Chaetocladium jonesii, Chaetomium

globo-sum, Chaetomium cupreum, Chaetomium irregulare, Farlowiella

carmichaeliana, and many other filamentous fungi.

Malt Agar 2%

(MA2)

Malt extract 20.0g

Agar 15.0g

Use: For the cultivation of Acrophialophora fusispora, Agaricus

augustus, Ceratocystis penicillata, Chaetocladium brefeldii, and many

other fungi

Malt Agar 4%

(MA4)

Malt extract 40.0g

Agar 15.0g

Use: For the cultivation of Chaetomium globosum, Chaetomium

indi-cum, Chaetomium funicola, Chaetomium megalocarpum, Chaetomium

murorum, Chaetomium seminudum, Chaetomium pachypodioides,

Chaetomium perlucidum, Chaetomium quadrangulatum, Chaetomium

reflexum, Chaetomium subaffine, Chaetomium subspirilliferum,

Chaeto-mium succineum, ChaetoChaeto-mium luknowense, Daedalea quercina,

Mycosphaerella ligulicola, Polypaecilum pisce, and many other fungi

Malt Agar 8%

(MA8)

Malt extract 80.0g Agar 15.0g

Use: For the cultivation of Moniliella pollinis.

Malt extract 20.0g Glucose 20.0g Agar 15.0g CaCO3 5.0g Mycological peptone 1.0g

pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Brettanomyces anomalus,

Brettanomyces bruxellensis, Brettanomyces claussenii, Brettanomyces lambicus, Dekkera bruxellensis, and Dekkera intermedia.

Malt Agar with 2% Malt

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.5 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation of a variety of yeasts and other fungi

Malt Agar 2% with Lupine Stems (MA2 with Lupine Stems)

Malt extract 20.0g Agar 15.0g Lupine stems variable

Preparation of Medium: Add components, except lupine stems, to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring to boiling Distribute 6.0mL volumes into tubes Cut lupine stems into 8.0cm-long pieces Add 2–3 lupine stems per tube Autoclave for 15 min at 15 psi pressure–121°C Allow tubes to cool in a slanted position

Use: For the cultivation of Chaetomium bostrychodes.

Malt Agar 4% with Lupine Stems (MA4 with Lupine Stems)

Malt extract 40.0g Agar 15.0g Lupine stems variable

Trang 6

1000 Malt Dextrose Peptone Agar

Preparation of Medium: Add components, except lupine stems, to

Gently heat and bring to boiling Distribute 6.0mL volumes into tubes

Cut lupine stems into 8.0cm-long pieces Add 2–3 lupine stems per

tube Autoclave for 15 min at 15 psi pressure–121°C Allow tubes to

cool in a slanted position

Use: For the cultivation of Chaetomium indicum and Chaetospermum

chaetosporum

Malt Dextrose Peptone Agar

(MDPA)

Agar 25.0g

Malt extract 20.0g

Glucose 20.0g

Peptone 1.0g

Use: For the cultivation of Aspergillus aeneus, Aspergillus candidus,

Odontia uda, Oidiodendron chlamydosporicum, Oidiodendron cerealis,

Oidiodendron flavum, Oidiodendron griseum, Oidiodendron

perico-nioides, Oidiodendron tenuissimum, Penicillium spinulosum, and other

fungi

Malt Dextrose 40% Peptone Agar

(MDPA 40)

Glucose 400.0g

Agar 25.0g

Malt extract 20.0g

Peptone 1.0g

Use: For the cultivation of Aspergillus penicilloides

Malt 4% Dextrose Peptone Yeast Agar

(MDPYA4)

Malt extract 40.0g

Agar 20.0g

Glucose 20.0g

Peptone 1.0g

Yeast extract 1.0g

Use: For the cultivation of Candida glabrata, Candida haemulonii,

Can-dida lactis-condensi, CanCan-dida magnoliae, CanCan-dida nemodendra,

Met-schnikowia pulcherrima, MetMet-schnikowia reukaufii, Phoma glomerata,

Saccharomyces exiguus, and Trigonopsis variabilis

Malt 4% Dextrose Yeast Agar

(MDYA4)

Malt extract 40.0g Agar 20.0g Glucose 20.0g Yeast extract 1.0g

Use: For the cultivation of Torulaspora delbrueckii

Malt Extract Agar

Malt extract 30.0g Agar 15.0g Peptone 5.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Xanthomonas species.

Malt Extract Agar (MEA)

Agar 20.0g Glucose 20.0g Malt extract 20.0g Peptone 1.0g

pH 5.5 ± 0.2 at 25°C

Use: For the isolation, cultivation, and identification of heat-resistant filamentous fungi (molds) from foods

Malt extract 30.0g Agar 15.0g Mycological peptone 5.0g

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring until boiling Distribute into tubes or flasks Autoclave for 10 min at 15 psi pressure–115°C Do not overheat or agar will not harden

If a lower pH (3.5) is desired, cool medium to 55°C and aseptically add 100.0mL of sterile lactic acid Pour into sterile Petri dishes or distribute into sterile tubes

Trang 7

Malt Extract Agar, Half Strength 1001

Use: For the detection, isolation, and enumeration of yeasts and

molds The addition of lactic acid suppresses bacterial growth

Malt extract 30.0g

Agar 20.0g

Chlortetracycline solution 10.0mL

pH 5.5 ± 0.2 at 25°C

Chlortetracycline Solution:

Chlortetracycline 0.04mg

Preparation of Chlortetracycline Solution: Add

chlortetracy-cline to distilled/deionized water and bring volume to 10.0mL Mix

thoroughly Filter sterilize

Preparation of Medium: Add components, except

chlortetracy-cline solution, to distilled/deionized water and bring volume to

990.0mL Mix thoroughly Gently heat and bring to boiling Autoclave

for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically

add sterile chlortetracycline solution Mix thoroughly Pour into sterile

Petri dishes in 20.0mL volumes

Use: For the cultivation of yeasts and filamentous fungi (molds) from

cosmetics

Malt extract 20.0g

Agar 15.0g

Peptone 5.0g

Use: For the cultivation and maintenance of Candida melibiosica,

Cryptococcus curvatus, Kluyveromyces species, Metschnikowia

hawaiiensis, Rhodosporidium paludigenum, Saccharomyces

cerevi-siae, Saccharomycodes ludwigii, Stephanoascus species, and

Trichos-poron nigrescens

Malt Extract Agar (ATCC Medium 109)

Agar 15.0g

Maltose 12.75g

Dextrin 2.75g

Glycerol 2.35g

Pancreatic digest of gelatin 0.78g

pH 4.6 ± 0.2 at 25°C

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring until boiling Distribute into tubes or flasks Autoclave for 15

min at 15 psi pressure–118°C Do not overheat or agar will not harden

Use: For the cultivation and maintenance of yeasts, molds, and

Fla-vobacterium lucecoloratum.

Malt Extract Agar (MEA)

Glucose 20.0g Malt extract 20.0g Agar 15.0g Peptone 1.0g

Use: For the cultivation of Arthrinium phaeospermum, Aspergillus fumigatus, Aspergillus clavatus, Penicillium verruculosum, and Peni-cillium spinulosum

(BAM M93) Compositionper liter:

Malt extract 30.0g Agar 20.0g

pH 5.5 ± 0.2 at 25°C

Use : For the isolation, cultivation, and identification of heat-resistant

filamentous fungi (molds) from foods

Malt Extract Agar, Blakeslee’s

Malt extract 20.0g Glucose 20.0g Agar 20.0g Peptone 1.0g

Use: For the cultivation and maintenance of a variety of yeasts,

includ-ing Candida versatilis, Cryptococcus elinovii, Kluyveromyces marxi-anus, Pichia species, Reniforma strues, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Sporobolo-myces roseus, Trichosporon nigrescens, Yarrowia lipolytica, Zygosac-charomyces rouxii, and numerous filamentous fungi

Malt Extract Agar, Half Strength (ATCC Medium 2418)

Agar 15.0g Malt extract 10.0g Peptone 2.5g

pH 5.5 ± 0.2 at 25°C

Trang 8

1002 Malt Extract Agar for Yeasts and Molds

Malt Extract Agar for Yeasts and Molds

(MEAYM) (BAM M182)

Agar 20.0g

Glucose 20.0g

Malt extract 20.0g

Peptone 1.0g

pH 5.4 ± 0.2 at 25°C

Use : For the isolation, cultivation, and identification of heat-resistant

filamentous fungi (molds) from foods Recommended for

identifica-tion of Aspergillus spp and Penicillium spp

Malt Extract Broth

Malt extract 6.0g

Glucose 6.0g

Maltose 1.8g

Yeast extract 1.2g

pH 4.7 ± 0.2 at 25°C

Di-agnostic Systems

or flasks Autoclave for 15 min at 15 psi pressure–121°C Do not

over-heat—this results in darkening of the broth

Use: For the isolation, cultivation, and enumeration of yeast and

fila-mentous fungi (mold)

Malt extract 17.0g

Mycological peptone 3.0g

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath

Use: For the cultivation of molds and yeasts, especially for sterility

testing

Malt extract, purified solids 15.0g

pH 4.7 ± 0.2 at 25°C

Di-agnostic Systems

or flasks Autoclave for 15 min at 15 psi pressure–118°C Do not over-heat

Malt Extract Charcoal Medium (DSMZ Medium 801)

Malt extract 30.0g Agar 15.0g Charcoal 3.0g

pH 6.5 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Boletus edulis, Xerocomus badius DSM 4436, Antrodia serialis, and other filamentous fungi

Malt Extract Glucose Agar (DSMZ Medium 735)

Agar 15.0g Malt extract 10.0g Glucose 5.0g

pH 7.1 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Morchella esculenta spp., Verpa digitaliformis, Disciotis venosa, Mitrophora semilibera, Gyro-mitra spp., and Mitrophora semilibera.

Malt Extract HiVeg Agar Base

Malt extract 30.0g Agar 15.0g Plant peptone No 4 5.0g

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 5.4 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of fungi, especially yeasts

Malt Extract HiVeg Agar Base, Modified

Agar 15.0g Maltose 12.75g Dextrin 2.75g Plant peptone 0.78g

pH 6.0 ± 0.2 at 25°C

Trang 9

Malt Yeast Extract 50% Glucose Agar (MY50G) 1003

Hi-Media

water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.0

Gently heat and bring to boiling Distribute into tubes or flasks

Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Malt Extract HiVeg Broth Base

Malt extract 17.0g

Plant peptone No 4 3.0g

pH 5.4 ± 0.2 at 25°C

Hi-Media

Auto-clave for 15 min at 15 psi pressure–121°C

Malt Extract Peptone Agar

Malt extract 30.0g

Agar 15.0g

Soy peptone 3.0g

pH 5.6 ± 0.2 at 25°C

Auto-clave for 10 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of Basidiobolus ranarum

and Sclerophoma pityophila.

Malt Extract Peptone Agar

Malt extract 30.0g

Agar 15.0g

Papaic digest of soybean meal 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Ascobolus immersus,

Aspergillus amstelodami, and Aspergillus clavatus.

Malt Extract Yeast Extract

40% Glucose Agar (MY40G)

Glucose 400.0g

Agar 12.0g

Malt extract powder 12.0g Yeast extract 3.0g

pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except glucose, to 550.0mL of distilled/deionized water Mix thoroughly Gently heat and bring to boiling Bring volume to 600.0mL with distilled/deionized wa-ter While the solution is still hot, add the glucose all at once while stir-ring to avoid formation of lumps Autoclave for 30 min at 0 psi pressure–100°C

Use: For the isolation and cultivation of osmotolerant microorganisms from foods

Malt and Peptone Medium

Agar 15.0g Malt extract 10.0g Peptone 5.0g NaCl 1.0g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Adjust pH to 6.5 Autoclave for 15 min at 15 psi pressure– 121°C

Use: For the cultivation and maintenance of Flavobacterium species.

Malt Peptone Yeast Extract Agar

See: MPY Agar

Malt Yeast Agar

Agar 20.0g Glucose 10.0g Peptone 5.0g Malt extract 3.0g Yeast extract 3.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Arthrobacter viscosus.

Malt Yeast Extract 50% Glucose Agar (MY50G)

(ATCC Medium 2093)

Glucose 500.0g Agar 10.0g Malt extract 10.0g Yeast extract 2.5g

pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add agar, yeast extract, and malt extract

to distilled/deionized water and bring volume to 500mL Mix thor-oughly Gently heat while stirring until boiling Slowly add glucose while stirring to avoid lumps Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Do not overheat or agar will not harden Pour into sterile Petri dishes or leave in tubes Note: This agar hardens very slowly

Trang 10

1004 Malt 2% Yeast Extract Agar

Malt 2% Yeast Extract Agar

(MYA2)

Agar 20.0g

Malt extract 20.0g

Yeast extract 1.0g

Use: For the cultivation of Actinomucor elegans, Actinospora

mega-lospora, Agaricus bisporus, Ceratocystis perfecta, Ceratocystis cana,

Cer-atocystis seticollis, Chaetomium trilaterale, Chaetomium indicum,

Chaetomium seminudum, Chaetomium piluliferum, Cirrenalia

macro-cephala, Kluyveromyces species, Lepista inversa, Torula dematia,

Tricho-derma pseudokoningii, and other fungi

(MYA4)

Malt extract 40.0g

Agar 20.0g

Yeast extract 1.0g

Use: For the cultivation of Arthrobotrys arthrobotryoides,

Ascospha-era apis, Bettsia alvei, CAscospha-eratocystiopsis minuta, Chaetomium

spino-sum, Chaetomium piluliferum, Ciboriopsis simulata, Dactylella

minuta, Dactylella rhombospora, Dactylella lysipaga, Eriopeziza

cae-sia, Europhium clavigerum, Issatchenkia orientalis, Moniliella

suave-olens, and other fungi

Malt 4% Yeast Extract Agar with Lupine Stems

(MYA4 with Lupine Stems)

Malt extract 40.0g

Agar 20.0g

Yeast extract 1.0g

Lupine stems variable

Preparation of Medium: Add components, except lupine stems, to

Gently heat and bring to boiling Distribute 6.0mL volumes into tubes

Cut lupine stems into 8.0cm-long pieces Add 2–3 lupine stems per

tube Autoclave for 15 min at 15 psi pressure–121°C Allow tubes to

cool in a slanted position

Use: For the cultivation of Ceratocystiopsis minuta-bicolor,

Ceratocysti-opsis retusi, Ceratocystis pilifera, Ceratocystis olivaceapini, Ceratocystis

multiannulata, Ceratocystis nigra, Ceratocystis olivacea, Ceratocystis

tremuloaurea, Chaetomium indicum, and Chaetospermum chaetosporum.

(MYA8)

Malt extract 80.0g

Agar 20.0g

Yeast extract 1.0g

Use: For the cultivation of Thielavia hyalocarpa.

Maltea

Maltea 40.0g Agar 22.0g Yeast extract 3.0g

Use: For the cultivation of Leucogyrophana mollusca

Maltose Peptone Yeast Extract Agar

See: MPY Agar

Maltose Peptone Yeast Extract Broth

See: MPY Broth

Maltose Peptone Yeast Extract Medium

See: MPY Agar Manganese Acetate Agar

Agar, highly purified 10.0g Manganous acetate 0.1g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add manganous acetate to distilled/de-ionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.0 Add agar Steam the medium to dissolve agar Distribute into screw-capped tubes or bottles Autoclave for 15 min at 15 psi pressure– 121°C Allow tubes or bottles to cool in a slanted position

Use: For the cultivation of manganese-oxidizing bacteria

Manganese Agar No 1 (Mn Agar No 1)

Agar 10.0g MnCO3 2.0g Beef extract 1.0g Fe(NH4)2(SO4)2 0.15g Sodium citrate 0.15g Yeast extract 0.075g Cyanocobalamin solution 10.0mL

Cyanocobalamin Solution:

Cyanocobalamin 0.005mg

Preparation of Cyanocobalamin Solution: Add cyanocobala-min to distilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except cyanocobala-min, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 10.0mL of the sterile cyanocobalamin

Định dạng
Số trang	10
Dung lượng	216,9 KB