[...]... influence chromatography and separations The main objectives of this book are as follows: Provide basic information on chromatography and separation science Describe the relationship between these important fields Cover how simple extraction or partition processes provide the basis for development of chromatography and separation science Describe the role of chromatography and separation science. .. understanding of transport phenomena and thermodynamics, an elementary discussion is included in Chapters 3 to 5 The discussion on chromatography should help improve the understanding of the principles involved in various modes of chromatography such as paper chromatography, thin-layer chromatography, gas chromatography, and high-pressure liquid chromatography (Chapters 6 to 10) This book relates simple separation. .. Wiley, New York, 1991 15 Ahuja, S Chiral Separations: Applications and Technology, American Chemical Society, Washington, DC, 1997 16 Ahuja, S Chiral Separations by Chromatography, Oxford, New York, 2000 QUESTIONS FOR REVIEW 1 Define separation and chromatography 2 How are chromatography and separations related to each other? 3 What differentiates chromatographic methods from related separation methods?... techniques of separation Industrial and scientific revolutions have brought about the need for development of a variety of separation methods, and this need is being met admirably by separation science As a matter of fact, most advances in chemistry, chemical technology, and life sciences have been related to advances in separations and chromatography A few examples follow: 1 The automobile industry and transportation... conclude that the science of separations is rooted primarily in transport phenomena It is important to understand the underlying mass transport phenomena of all separation processes This will allow us to describe and explore the limitations and performance of alternative separation techniques and the factors enhancing and limiting separation power Separation procedures will continue to change and evolve with... processes and thus help to make better use of these powerful methods In this text, fairly detailed discussions of both the fundamental aspects and the practical applications of separations, including chromatography, are presented, with major emphasis on laboratory- and analytical-scale separations Small-scale nonchromatographic and chromatographic separations that are commonly used by many chemists and employed... number of different kinds of problems in separation science I DEFINING SEPARATION Since separations are important in almost all our endeavors in science and technology, the word separation is well known, and virtually anyone working in this field can come up with a meaning for this word However, it is quite difficult to define separation precisely and completely.3–6 Separation has been simply defined as an... contributions of chromatography Examples from these and other fields are included throughout this textbook This book will provide the necessary information to improve the comprehension of separation science It will show how chromatography relates to separation science and describe how simple separation methods have led to highly useful chromatographic techniques that provide high resolution (Chapters 1 and 2)... transmitted and how we can treat them better with suitable medicines 1 RELATING CHROMATOGRAPHY TO SEPARATIONS 7 4 Our understanding of a variety of biochemical processes occurring in the human body became possible after development of separation methods such as ultracentrifugation, chromatography, and electrophoresis 5 The studies on the human genome and proteomics owe their success to separation science. .. TO SEPARATIONS I DEFINING SEPARATION II EVOLUTION OF CHROMATOGRAPHY A Definition of Chromatography B Similarity of Chromatography to Separation Methods III SEPARATIONS IN EVERYDAY LIFE IV BASIS OF SEPARATIONS A Physicochemical Phenomena B Utilizing a Desirable Physical Property V MODES OF CHROMATOGRAPHY A AdsorptionVersus Absorption B Partition or Distribution C Exclusion D Ion Exchange VI UNIFIED SEPARATION . of chromat ography and separation science. . Describe the role of chromatography and separation science in various fields. . Discuss the role of chromatography and separation science in development. OGRAP HY AND SEPARA TION SCIENCE Th is is Volu me 4 of SEPARATION SCIENCE AND TECHNOLOGY A reference series edited by Satinder Ahu ja C HROMATOGRAPHY AND SEPARATION SCIENCE Satinder Ahuja Ahuja. the under- standing of the principles involved in various modes of chromatography such as paper chromatography, thin-layer chromatography, gas chromatography, and high-pressure liquid chromatography