[...]... 1.6: Techniques Industrial in Biotechnology Centrifuge Manufacturers Location Company Alfa Lava& Inc Amtek, Inc Baker Perkins, Inc Barrett Centrifugals Bird Machine Company Centrico, Inc Clinton Centrifuge, Inc Commercial Filters Dorr-Oliver, Inc Heinkel Filtering Systems Krauss Filtering Systems, Inc IEC Division, Damon Corp Pennwalt Corp., Stokes Division Quality Solids Separation Co Robatel, Inc... SOLIDS SOL IDS d Figure 1.3 (continued) 14 Separation and Purification Techniques in Biotechnology INTERMITTENT e FEED EFFL Figure 1.3 (continued) Introduction 15 Solid bowl centrifuges are available in five basic types 1.3b) and is called tubular bowl (Figure The first type A continuous operates like a batch laboratory centrifuge feed of material passes through the machine As soon as the bowl is full...2 Separation and Purification Techniques in Biotechnology a bacterial fermentation for single cell For example, protein will produce a broth of 3% WV in which the slurry is about 60% (by volume) wet biomass and 40% interparticle fluid When biomass supports are used, somewhat higher operating biomass concentrations are possible and, in waste disposal fermentations, the... applicable to those fermentation separation technique is Introduction Table 1.7: Industrial Equipment Company Alfa-Lava1 APV Company, Inc Dedert Corp Distillation Engineering Co, Goslin-Birmingham HPD, Inc Industrial Filter and Pump The Kontro Co., Inc Luwa Corporation Paul Mueller Co Niro Atomizer, Inc Pfaudler Co Swenson Process Equipment Unitech, Div of Graver Co Henry Vogt Machine Company 23 Manufacturers... cooling in the larger amount of solvent removed by the evaporator and in Should either the steady supply of heat to the evaporator of these techniques prove inadequate, the crystalline product may be salted out by the addition of chemicals 24 Separation and Purification Techniques in Biotechnology NUCLEATION TEMPERATURE (T) Supersaturation to obtain crystal nucleation may Figure 1.7 1 cooling; 2 adiabatic... level of selectivity to ligands covalently attached to a solid matrix In solvent extraction, the removed distribution between equilibrium an usually water and an organic liquid electrodialysis Electrophoresis and techniques that separate charged molecules compound immiscible establishes solvents, separation are or ions using an 6 Separation and Purification Techniques in Biotechnology electric field... nor forms a precipitate during the evaporation Introduction 19 FEED I VAPOR -CONDENSATE PROliUCT FE)ED b a STEAM PRODUCT c (a) Long-tube vertical evaporator Evaporators Figure 1 S (c) Propeller calandria (b) Falling-film evaporator evaporator (d) Plate evaporator (e) Thin film evaporator 20 Separation and Purification Techniques in Biotechnology STEAM STEAM PACERS INT VAPOR AND CONCENTRATE TO SEPARATOR... and Purification Table 1.4: Industrial Techniques Filtration Company Allied Filtration Co Avery Filter Co Bird Machine Company Carl C Brimmekamp & Co Denver Equipment Company Dorr-Oliver, Inc EIMCO (Div of Envirotech) B C Hoesch Industries Inlay, Inc Komline-Sanderson, Inc Lenser America, Inc Peterson Filters, Inc R & R Filtration Systems Serfilco,‘Ltd Sparkler Filters, Inc D.R Sperry & Co the thickness... Adsorption Gel filfrstion Affinity method9 Distillation Membranes Electrophoresir Differential freezing Evaporation Membrsner Pmcipitalion Adsorption ion exchange, clf%ily erc Frocra.ThDwing-cryslalliralion Figure involved products Schematic 1.1 in the separation (Reference 2) of the processes and fractionation which may be of fermentation 4 Separation and Purification Techniques in Biotechnology If the desired... DRIVE CARTRIDGE-TYPE DOUBLE MECHANICAL MAIN BEARING S MOTOR VAPOR FEED NOZZLE NOZZLE ROTOR HEATING HEATING JACKET JACKET A: BOTTOM NOZZLE B: HEATING HEATING HEATING NOZZLE JACKET LOWER GUIDE CONE DISCHARGE e Figure 1.5 (continued) ROTOR BUSHING NOZZLE GASKETS Introduction 21 The falling-film evaporator (Figure 1Sb) is similar to long tube vertical evaporators except in the location of the To operate properly . Dechow, Frederick J. Separation and purification techniques in biotechnology. Includes bibliographies and index. 1. Biomolecules Separation. 2. Biomolecules Purification. 3. Biotechnology Technique of the processes which may be involved in the separation and fractionation of fermentation products (Reference 2). 4 Separation and Purification Techniques in Biotechnology If the desired. liquid. Electrophoresis and electrodialysis are separation techniques that separate charged molecules or ions using an 6 Separation and Purification Techniques in Biotechnology electric