advances in rice genetics pdf

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Preface

1966 $89 elon toni 2003 This has mainly been asbieved through te applic

ton of principles of Mendekan gente sed onvetiona plan beeing methods

{eld poteta and mukiple resistance to bite and abode stresses with improved

From beings por eosin size, west an tomato for genet knowled,

6 socely asthe 1980s sce his Dosone a del plan for moteur gente

‘ore higher pl! for molecular and ela genetic ses Notable examples in terization of genes governing vanousspronomi as, These advances covered throw ince genetics and breeding, “

This book Adances ie Rice Genes, ithe supplementary volume of Ree secon) gene and Breeding of agrononeat,() genetic divers, eo ton and aes introgression, (3) molec arr, QT happier sre selection (4) genomes (5) gee lation an function 6} ine ul ad Inthe ist sexton, 38 papers over the gent alysis an ierane of wioúe gganonie de ue te mule đedliỹ:fediy xsomutie hyb eke terns Fory-rix papers desrine te ws o[muleedr maRew inthe analysis of

‘ste diverse evlition of clive ce, monierag oi nkogrenion, Sndidetieation of wild specealleleQTLforimpoving ri neading advances

in ice cytogenetics tough FISH wecigues More tan 4 papers highligh te

‘pplication of molecular markers in tugging major genes and in markerassised se Inston Sever popes deal with he entation of QTL or heading des last

lance: and amylose coment As many as $6 papers cover the advances made in

_sstoies ad soln ad unc of ges Some ighlighsaclade TNA e7, terization of DRED genes MADS-ox gene, anspor pees, mechanism for

<eferse signal transduction, bisyoers of prolamin andthe gene propensity ratlnoficecarying gene or resisance Boi and abit stresses DNA, transformation, and mat atachment regions fr say of transgene expression

Th ast ston overs eight papers the genet treo ls populations

‘Wehope that thishook willbe valuable reference forth entific ommuiy ngage in genetics and reding of, with phasis on oth ora and eves

Acknowledgments

Fourth Inertial Rie Genetics Symposim: ohn Benet Swapun Dat, Mike Jackson, Zhang Lad Hel Leung We Wold so Iie To ve spc hanks to nowledge, Weals thnk Yl ranger ElmaNicls, ly Aunt, George Reyes, and Dane Martine for secretarial help and provesing te manos eae Communion and Poblsations Services, Food and Hoang Sevies, Vis tos and nfonneuon Series, Physical Phin Series anda ecco

Genetics and breeding

of agronomic traits

Comparing agronomic performance of breeding populations derived from anther culture and single-seed descent in rice

'EBIWe lo lơ Cho Do HO Hog SH Co, ar Hh

Ts eter wat conte i cere he bee fin far cue AC) a reso

‘la rf ptemone fylostd vats ng gon cay Naseer vor mi Inmencorparsom ct yetcongrerts eeay Ye reba ies eee age at ert,

-4Ss0 nebee hagadesoineeirghetcd,shợvĩeaefs ferte dee ats arcelengh, runt of gars ancl etn), reeaing ost srg 0e eefsnetssgesnelrrsve Cd

‘ears of bes tats cob posse by eta 80 SSD mets Theres eras porome as.n ck eles arte wp omig tes ae eran between Nobeechgretha, Owl he AC rebut race access eer rte wilson asa

ex esnesin th cess ne sed mph tate AC ret arb eid os beng

regen

ing mets speedup the Beeting eye nthe Korean

re for selstion rom ter ule japon roses,

‘hough there ae sil sme rolens i indica genotypes A

‘was release in 1988, These varieties currently account for

‘sound 25% of the e-rowing area in Korea Despite the

Tmited understanding of he penal fercuvar development

‘ramer etre Baus of Hs neren factor, sch ge

notype dependence androgeness he deletious effet of

1993) and only one chance of recombination before ftion

inthe being lines derived fem anihercuure Inti sy,

we sn 0 compare te bresding nes derived row aber

-fangrcsehe phenomenon for ella wats and gully

Meteriasand methods

Seed descent (SD) nes The preter in agronomi pnt

line were discarded and ced arvesing wos done erie

Api plants The Ry eneraton was grown frst mihi

‘ston nthe rice fie anda ol of 381 nes th Rene seeds othe same cross above were grow inthe fei in 194, Six unre puns were randomly sled and singe sod

‘ested om each Fy plan, seed) ad 916 les ofthe F, eoeraion were prodced

lies for yelled ats and SSD populations was _valuated inthe ie The phsi-chemical vals of ea

{he laboratory, We selested 4 superior lines with good agro

toni as by visulselstin nthe ld rom ach AC nd Feplieatons The mide 10 pans Were used for dia colle: thn Heading dae esl length, pani length number of Perplant wereinvestpate ad tail analy

‘er the AC poputon nal vals esi higher

height, and good fertity The mean values of chose tats in

“ole 4 anand ange fri an compan fr Ee dad om ant cue AC angled escnt

Tete 2 Petcntge of vans ne fr Yd ay components in athe (A) ad sgh ted desent (SSO) es”

the AC population te lsc the mid-pucn vale Te

tsrononie vole was ower tn he mid preil ae ih

3 vi range of vation i the population iocating 3

AC poldom Tle shows he spss fae

Siveins fr esc ait wii ach AC and SSD population

rst nes was iho ith SSD population,

Ii asigiea ference was mi hte een te

‘rh hikes phytochemical coe

Scene aa igen vale, and aos omen st

oan tee for dermming hat certain beeing ose

Teton meta cule fete asd ints eine po

rosie genotypes aowgh genetic variation an the abso

Inte ruber of tanagressive les in he AC pepglaion were

‘Seceal ner tre resin depends on he eahish-

teat ofan appropriate bring population Wenzel ea

‘obtain sper lines Arai, he probably of b

‘ening the bet eccrbiaant on be anced By pedeng [AnFdeved DH population may connin opt 0% mone of

‘hehe eecombinante han the, sso, old test of soloctod AC and SSO tines Fowy-tve ee lines were selected sully fom cach AC nd righ lngand god panel shape and high eit Table 3 shows the mean yield and yet component of 4 selected tncod: No sgificanifernce i mean aponomis perio tance was dete etacen AC and SD withincach sled ing the mean ye he hs population from whch se thn wat mad io he pevious Year (Table he 48 selected 24% inthe SSD method although direc comparion if ers Although visa sckton fmt conieredt be ae ing method nth roe wed inti

‘AC and SSD afe to lnporant beeing methods

2 Maa ryan ald compat of 5 saat Ina ad tp he Deng nes on A nt S80 yopatins

[AC method crsnly has a lear time-saving advantage over

ing t yield ih wheres SSD took 6 year

‘veal gronomls perfomance, cling yielxelael ats

‘spronomi peformanceo seletedhresting nes, Intiscon-

texan ulture ca also bo effectively soda boeing

stand he een efieney of ante cule

Advances in breeding salt-tolerant rice varieties

8.maa, RK Sigh ard Senta

jogs as ban de in wed st lati lrg fo aa rr land tn

‘alsin, an shane sls tg seater O32 tart ores deme gai Sasa esa esse OSS, SRD nose soa Menetrg saat — - gee

{SRLS safe gan stcirroe ae apne aire ae ss ars OSA2T poses -a3ioerrel o4 tin trdœoeoy uh esa ee sed ces, CSRZT poses

Ngitas anc arch at press mong ly Were ces rece a gi tes angen tears N CSRS, itespr uly that fox as a sede sted

(ans uh creates ene, nigh eel eestor ch cokrg, emede ed red mou cnet esputundl ee geri gas and get eros xe al

tte cerns tet ren aon Kans Ppa, peste ern ‘Wena cnsnes det peopel manne ina on eee tatiana aa pose

incunedmnngelah anh and tancd al ers Hower ro srg ranepel anv

Scare Wy epedici-ae slr se a gan ym Som he ar roraae gre fers EC

tebetconbres ane ad aly wirance ated

Dredg for sa lerance ic a more promising, enery-fi=

‘fen esonomial and seal acceptable approach san mie

fee and ape emp fr slated als esa of

inherent gence varity fora tolerance” Using hs ae"

mens that an be esily adapted by resource-poor farmers

‘Gemplasm improvement and viet development

Rk ecnnmendd she fitcrop ding he esanation

fit dented the tationlslolernt sari CSR

{Damon 2 dopo een rain ell ection Wess

“he fine dar Nghicing sola ab ea

sate re, Clo- ns deve) CSS

‘i, Kea ian iy (EC 6-1) dr letanglatei ra#edmameemer high alin (9 4-10.) ands

Sa Tnhh skal wi Under mrt rest yes

Soest Reco furs whe ae ul

‘y CSR 3, derived fom iethec-aay gross CSR Bá 3707

{e's war mln in 109 for gt line)

{and tela (pt 9.2109) nd saline ee

Sporn 9 sess Gran il ngs fam S319 67 eh Y

cơn Nghyemg leuleraninrecvarg,CSRĐ) clemed

TY CYẤC Ìn 199 for aan acon sine of he

10) stresses Under moderate deteriorated saltafeted ois

iydea40-S0 thas Ths vary poster her te

Shi anh Toa pes CSR 0 he ft bse

‘alvotenn vwley derlopel su eaumnenll Mai

‘scented ras with ood head ice resovery (39%) high her-

‘elcoagaton on coking (KLAC) meme gltaiog

30s syllshanoạcofBou 2% ove Ta Bast “These ap! vrees coal sine se

Panel, Pvc, Pot Gn Ker ony) Wa a

‘pela Famer pureiption nade rose slot

prerlese and advanced bresting ines nso sail of 16

Ut of ir Paes as edhe enston of CSI, Genetics ofslintytleranoe andionc uptake

high mean, pea unit egresson value cb) and lowest de

‘ation from repression (,* under multiples envio are CSRI0.CSRI!, CS)3,CSR2T, CSRI, Pollal, CSRI8,

nd CSR for grain il, led grains per pani low spikes Ina stad evel hat gai ye pr plant was post tively and significantly associated with nue of grains per

‘oteent spike sterility Pah coefficient analysis showed that

‘he ret effect of panicle weight fer ler, straw yield

‘umber of grains per panicles an panicle weight exhibited

‘maxim direct ffect in alkal soil Panisle weight was not, significant correlation betwee sanity sore atthe vegetative Sage and the score atthe reproductive stage Idi nt havea rin yield Grain yeld was highly and positively correlated

‘wth file grains pe pant filled rans er panicle, panicle tive comelaton with percent spikeetsteiity and reproduc tivestage sali tolerance score, whieh is highly desirable Estimates of genatie parameters showed significant a vend ponadive gene ation or salinity wlerance 04, 1.63 for salinity olrace sere 0.88 for K* absorption, ad 1.4 for Nak ratio The average dominance 3s within the CSRIO and ESRI for reproductivestge salinity tolerance sentnled han recesive alleles in Nona Bokra, CSR, and (CSR in that order We-Vr graphic analysis soggesed the in

‘volvement of oth majo and mior genes fr al the wats in

‘magnitude for Nak ratio for Hoth aka (0.658) and sane sls 0.630) and was high for rai ie i ala (0.347) and saline seis (0.569) Spiele striy © and number of filled Giifig’s 196) method confirmed the significance ofboth

dive and nonadtine ets the former showed grr

impetance nthe inert of he us

te carters sled Gr els the prime sence of

bree Out IF sce PKaMRORC79.89),CSR00

ml ren am cross PokklilR28 (4,31) over the eter

rot nll oi wees CSRERREE 141710 CSRIUU

‘€8R13, 24.53) over the better paren in saline sol exhibited

hesertei sot

Feceinaon teeing

Brceding following mule crosses etc contasting pat

{nd population hve sn generated uber ean salk

foleranee- The geauypes are grouped it ive catego: (1)

‘tational sa-olerant varieties al lankases, sổ (5) hạh

{eling ad fine-grain atc,

nisms then ts hybridization i atemped with anther gop

‘of varies ifeing a mechani, Some proven sate

rangement fora sossing step (Singh et 2000) The

esleran).minimam perdiy uptake of Ns aks moredaye

for LCase) igh upake of Ker day, gd inl vigor,

sions ahh depres of Ni exsason fom CORIO, CSRS,

(ESR, Has, PRIOR, sai MIs8 a higher ope of

'SR26B: and tolerance fora longer time for Na*opake from

Bau370, CORI, CSRIS and Pokal,

Condon and suggested future research

‘ult oerce explains the alte level of ality

st growth er sly isthe het indian of

Ie lesel of sal okrance ofthe gewonype toe ntone genetic huckgrotnd hy recombination,

\Wervggest that ture research involve

Fy anther eulredrived dvbledhaploid popula

ss Pores coortat,

Retrenoes

‘Mishra Singh RK 1999, Fine-pain sle-olerant rice varies

Isto os Bos Pits, 2 hy

‘Sigh RK MiB, lwo TY AR, 208 Seseig dec

Notes

Breeding for salt tolerance in rice

An Shere, SMA, 1 Poe and AR 6

Salary segs and ugar ae abo aorta fet te got ore fs, ity ose en berase st ee gan ae lest pee ls, Eats ea

‘neysigea vats we cere charts fon re tea fot e care sa i@mno

‘uhetroric yl ray argent ‘icon av stun aceundstinbe seeing gona osesangs sterner sca sho et sbaueten passin maang a

ie utatecep espns slr cantons

ier eidence ggets tht mst rice vrs exhib sl

Sees, but that vant erences tendo maaeshem=

[NaCl Yeound Flowers 198), The pan yet many sat

ies i redhced hy lf at on BC ofc eqialet oa

‘ue penal of about-0.23 MP on 0 M4 NaCl

‘vy, a wie inge of varaton exis amon fret ice

‘altivarsto combine desirable charatershas had on limited

Sucess because know edge ofthe gente hss al le

nce sited The msn expectation rom plan pilots

‘al (197, Soch makers ce characters hat are assole

‘Sod with sles re ely sentie, and can be wad for

"There is a nod to evolve rapid screening ethos

lune promising stain and sibsoqenly be al tờ cơn

Inany tases decsve ane in peformance at ater goth

leat mrlity

mm."

iinet le use death rsa rom in excesive ace

nce exon a avery ety stage of plan devehnpment Two

westoldricesedigs own nHoglan sluden ước min

Twkuthecame more severe wth ime, Alte clivarwere

sete adversely fer? wk of gvuth 10M and some

action ingrowth The response at his stage ares wel wih

‘has IR6, Shah, ndShas.2 hadow leaf morality er

ek of prow t 100 mB and igh yet (at wo ven)

Seedingsuvtl Fortumplated se, itl survivals ri in determining Inter erormanceScalings ht sarin thi stage are ox ete prodce gan alfough pkey canotberued

Su Secling suv al ater plating coud some extent be compen ory ang old seedings (Sw ont

‘ernie 6 wh od, Ansa tl 1993), bt itl wowing terms of sk nati, cuvare Gana White, Nos Bot, TRS, and Shu? ba eter seeing sre whereas TROE

‘veh tao en,

Lest chlaropyt Chorptyt degradation leading kinely to est det ss

‘line conditions (Yeo et al 1991), Unde similar conditions

tnd in some ese, however, an increase in elorop per

a

i rs has aso eon reported Resse of eed sie and

HH packed mesophylcels unde eline onion Inn

increas or shows no redton nip grown onder

san especie ofthese, ut erat ofehbo-

a ato was generally observed to contbute to Pete tolee-

Tati at corp ince naen 1 afer ams

Sodium ute and Nak ratio

!ajstment, has hnfalefecson plan growth, Large iter tndinavaita diteencesenstin ce ines or titrated Inulin nee shoot ero eter under sine contin than tows ht are unable to do Tita can slo Be sed Ine of our sts whore whol songs of S92 and TRAC wore harvested 2,3, 4 wk ae sailzation, t

"vá huerved(Tble 3) tha sodivn we crete and po ered to hve an vested ow inthe sensitive TRAC,

‘whereas th tolrant Shue? managed ont he bung

Distribution of scan betveen ees Regultonof the tansor sed stdin onsin he a fos organ he pnt and within tcl sa portant a: xedom i getrally mich lower in young leaves than ino

‘ones This advantageous fr sal oleae inst ss

* Shows the ime ours of sadam eaneentaton in india teaver of two vartes wih contesting salt less, The

‘ore elev the eltant Pol in which ole eaves

‘cu oko 100 mM Ky weigher 3-4 although te [ed with ait sk oleracea that could he wed a rteron for evaustig tolerance at th seedling tage which

C356 8sand 12a santo ith 0p Halt 3

a te aon

Peeenoes

ota es Epa E San Pi Zaborshy

Notes

‘uo Agni, Tunkjn ati), wera AR

Genetic analysis and prediction of heterosis

(CHIL Vipera Mes he, 8.04 ama, MS Ramet an, at

Secon ot state errs tore tee eto orto nba pet acs amar

‘onpontl eteas bes Resstson 1) guage overt ereteraypx etna M21 {pres etrcemant fren we ete nb opera, Torres tase car foretae beeline Seresuea based on UT iztedbjbf£ iso medi ed ago at Rss hee guid ed a 2D ole we dD

lontovaton mem are nda detent sas le tn Toke eres rete

8£ te em, org 3 vey Ney ees) ad peat) coe ging ea, Or 20 (Gus aliisnoc arnt pehseotehtrssuthayel ataragee otha ee Detes ed

‘ek at one rate fe at ters ness, © geal ap be rotoes 288) teang Fetes leo ơn 0n ren go cngBrergte re she nabs om hs gow 1 ee? Petes Wer ln or eg2 9t 23 sete fom 2 ep rine se (MS)

Ie RODE2SANG, 3662, ae sues stn RSI204 to ae the eal ely ered [iowa ese, TaF, cuss vet ruse agin een, R€OE2eMNG 3255

Indeed ta erty 22% ate estes wow et es came ty Fy mpeg Dak restates goed by ete man oe re ey 288 of cose MON a asa honed

‘pian htc evr gly for wich hay wee ane, arg at hen te foes a sponse toss,

tmagnitude ot heterosis depends onthe chic of appropriate

‘eal lines, Sleton of mitble donors poe part

‘rid wed om ie parents ar always been a primary oboe

‘veinal hynidcropbresting prognans ining ie Only

few cyophvmic male serie (CMS) ines could ews for

foremphasizng the slocon of mal parents resort) Se

‘a methods sich sp se pesfnmance, combining abi

‘il dia and DNA-bosel marker to dy the gence er

song pres and Hetero (Melenge 1997) a 0

“Teretore,the development of unetbod for choosing poten:

"The gente mechanisms unig eeoss ae argly unknown, Wereorton the eaonsig Between th at {ene responnbl for heeess om intr hybrid ots Progenles in seled generations

Mattias and methods The materials for the ist et of exprimons compris 16

In Inga, The 16 restorer were evalsted inthe Hk 0 randomized lek design dang the 1988 aod 1996 wet {nd spaced 20<m hetwcen coer and Sem tween plate N Tnwnnigogninykllm *andotsetevlonsoncevenlyieM the

Me H020 183

were recoded fom five domly slated pants, Data l=

Iced on eight traite—plat eight (ET) panicle number

plan! (PN) pail length (PL) numero erie pte

{Siskel fri prent SFP), I-grin weigh (TW)

Sel ied plant (SYP) and plo id (PY m2) were

‘sed to elsif the estorrs: ial, overlapping soups of

‘estorers ieooire foreach al asd on Dance

tril) war computed foreach Inefllowing metho ets

y Arinachalam and Bandyopadhyay 1988) Using ean and

lwidsinheratonal testing program were vst o en pom

ing restorers bose hybrid combinations showed comer

“he materiale forthe seco st of experiments come

‘ved fom IR62S29AAVGL 1068) and the ema pres

TRSDR2OA All he Feros, sing the ong hybed

GRODSZOAWGL 3962R), were eal i 4 rnd

lind in ve Selon ows th aroun sping of

Solem an betwee-plant spacing of 15 em Datum il

ineach eny Data on grin ye nd pkele foi were

so ent Ree yb

Fesltsand dscussin

Table sens the ib fr se DMRT

‘ets ter it that were cmon sh SP and PY

2m, Amojonty (6-74) ofthe etre wer ound to bei

Counc for intense and In eta eretdelr i

aril the dsbution pena Te es con

{heanalysis, nearly 75% ofthe restorers that were found in the

{enn exit ef tard evan en he ene tonnove nor oto the lam prop, Soh ak nrcange

yell advantage of 1.0 1h oF more over te biahest-iel

tot yar eng inding 725 eps These Singer themed gop soe, th eo Ste sai 394, mpedang Thee ings Posed

TT ral 190) Aca ok tthe yb ov

‘htm ny tent aon ve Sera es tom hed poup Alou hese on ia ferorance Te 3 The ean ses tose ne {Segre all rts were away hee foned by te

xe cletelbaels thất pưirnoneeneelot day hợ ess inary bis Cony to ur caer et one Fe Only sor ot of 8 incest pomal ey ae byes wes eine o showe ony Pat

{nal he segregating generations to devclp ne inc the

'ediiyassomto toa nawaeialy cote esp tans were ely sete he wld bore CWA) ye

Tat 2 Maan comparin of toe catagre fr variate

Tenney

Sraoled ny tn dominant gees, EWA) and Rt

{REA lated on ehomonnnes 7 and 10, respestiely

(Ghar et 1998) ners te oecutence of 2 re

frequency assed in conventional eserssoinva¥ing

‘hes an ele beeing lines, whinge om 20% 025%,

WEL 39628, Thee obsratons ha many implistions for

yhid bresing, Fs, he nolo of eres fo EMS

ing new restorers, Sevond he chsrvaton tht the Svved

‘were nerd rm the hybrid an hee progenies down the

Relationship of, parental Sonetic diversity with heterosis

in two-line and three-lit

F7 TT ee A Cel a, Rta

eo nfo ta ban aon htoebien.Ne metpedte ae s (Bsa heres fr ere uaa

38 eine and 13 ie F, eye Tre ren res wed eresemed etre gree ces ne Pp yn ete pF, haters)

esis ead sme scares ros (ER fetal ret: prtmare (nesses Pe Sisto vena pe ob gc feted at he 1% pew We toe CE

aut er pr pa ~ 031 ads ren ~ 0.30"

as gin yl 0304 8:

{Srets wnhacoss atte openly inline ec re, Mes Wer ech es aed

‘Neos texte Petre gear ote we Sane Tcafreinee = 05") rata Peart blespe part = O40", relanegladrl = 077) ates posal Elvin oiee fargo 048196, ngpsinga atest espe ene SP ea rar pera ctr Heats prorarce of i yes was Mest gan ae eee

‘Shetty 3620 an4 256%, msec een heen ten Mees sh aes

nest and simpler DNA ase markers have sim efor

{ordi theperfoeance of hrs based on molecular data

ở hat can ove as {oolinassessing heaves of parental eulivarsatthe DNA

‘nent ner baed on phenotype alone

TT

tree production and ai seleficeney bi been net

ingly seognized by oth he poli and private sectors re

icing the performance of ahyridsing molar tecigiee

‘old expt the develope of highly etre combina

Ins variod in liferent germplasm used (Sagi Maro et a

1997, Lia and Wi 198) The lear relationship Between

nepoted

Meteraisand methods

“Twenty-five parental euivars cotlrdng o S chaptanmic-

oncie male vt (CMS) linen 10 restorer (R) lines b

‘hemosentve geneterale seneCTGMS) ines and pop

lar Philippine inbred vate wee usd to devsop 48 thee

inet length polymorphism (AFLP) SR and ano amp

Hed polymorphic DNA (RAPD) markers Redo 1998)

flowing systematic plot arangementandrandomize om

etc blok desig wth our eck vas — ire PSBRC?S

for 10 vegetative and reroduetve characters—plan height

‘mats eat reaindee (LAT root ength oot veght năm

‘One bundte eight and 45 SSR primers were 0d te

amplify SSH loo as deserted By Yang eta (1994) athe poe

spstvely.Distne DNA fragments that were separate ih 8

‘Se denaturing polyctlamide ge ant deeced sing silver

ss was perfoaned following the UPGNEA procedure at the oper rogram NTSYS-PC (Rob 199

Reulland đsasson Forte of $5 SSR primer ected polymorphism mong the paral ultvars eed develop the threslne hybrids

‘Cister analysis using he poor ni separatatboCMS level of genetic similarity (Fi 2 dating the presence of substi ete varton te parewalcaltvasthteoud

be exploited to develop hyde With wide genetic hse

‘of hese pares showed low to intermediate heterosis fo plant

hah, atu, harvest inde ad grain yell const eteoss bạn or ot length 0 Weigh ad au

‘Cotltion bresen SSR heteozagog sed on 43

‘nahe ol aneeot performance i the 48 tee hte (ra dle) LAL (r= 0.39"), and pan geld r= 0.30),

‘when al Rs were wed in the analysis (Table 1 When ony

Tate 4 covalaton bean arnt gaat tne an tao đợi

{ata 8 taetoe Fy combo age ru í 2089 xe mỹ

Inds wt pose hte for ah et wee insted in

te amlysic henifeursoreldom xe bred LAI

“Thang mơler hectrygelye preml re deteee

tas bon cpr 1 essed whos fen

the sy wo grr ow or ot tet eae

‘nn th ctl ns asd in developing he vo

tne hb microti hetero ranged fo 03

{00.6 Hecate peonmanso! hp warhghe oga

—

—— rie asad on 4

eteamgety (von tals

femplsn, However, isgificantcomsiins were moa sre between pretzel stance ad beKres for {ivan Wo (1998 tht either molecular gente diversity of rca its or heterozygosity athe Fy appa Be god

‘Several actors ppc nlsne the compet elation

511995 sagged ht the ane of DNA markers col inked

to speci rate sichas ld ndterine specific tea osty may be more elective predicting eterote pro

imaee, However, ths wold require prio oomdge of the

‘romosoma oeaton ofthe gees contoling the tts of

inorent hough uantstive at oe (QTL) analy nd is

ing programs: Furterpore QTL expression cou ry with

ohgosicbekgrenndomlerưoanen(Redoi ad Mack

‘hereby limiting the general useflness and applicability of

‘marker data for betes prediction in ce,

Perences

Redo ED, Mackil DJ 190, Quaniis tat Mens analysis ot

no vtaneiraeieixioehesiraik

Cantera (Asi UTC Passing 7579

Sia wa MA, Yang OF Zhang Orvis KA 197 Ctr

‘oan Q 0420, Yang OR XG La KD Sapa Mao MA,

Notes

‘Stable high-yielding ability of japonica-indica hybrid rice

‘eka A ti, lag oe Haka

‘sept pneste sab ryt ily tones ides bd ce Ne deepen nthe S5 gre emacemratenc iver 690"ien2, whch’ eke tect re HL

‘poesia yor HPL a PRS ds wer cetaped by cosng panes CS Ine ME TMLL nda uth Hasta ar 8TH haZUEDlfesiafranlieee 3y teagrg37%hgØr 2 {an tate he apes cwck Nopetse, Horns ns ced ane weit san wee

‘ets ove slr tera ung eben be genetzEerl3ekln#gsoer2vreg NT yes 3.2 fa, 19% Đen he ge erect, Fuh The el of he era Ni Inia Ua S81 as inst re sone a at oe cia ae Pe ican ye shoved {Bat part 3p, ge pvc and geek san le paral hes ape) a0, o4

‘Sergogrgresies ou ite tetanic eee Oe tonal sa aft Pe opanchs Inds yes new Meh yig wth combed ge chaaconses ct eonca and a ae

Hybrid sce is expected ob igen Since aden ee

Bestade inte county Marayane 198 Aough Nybed

relueet ileld tiity(kchasi and An 198) Hens

hybrids can have normal gain ripening when one of the

cts he gene, Kak etl 1992 foun a pon

Mosever stable super nh ils ave nat een acleved in

std oar So est developed high-yielding japonica

Mattias and methods

We fit Sevcloped an improved japonica ne —TMLI with túc St gene from Kye SNL 2 Kynle 490

‘Seveloped by Tkchashi and Araki (1986), We then produced

the mae sei ine ms-TMLI by using a stops male srl (EMS) ie developed by Shiso197S) THe CMSTine Japoniceine TL Japonica hybvids THR and HRS

‘were developed by erising mS TMI ad ioe Habaak

‘Sey high-yielding varoty developed Japan In ont he

pate inca Uke 38 is reading ie developed fom a

‘Sombintion between Chinese ii valelce

Tatons ving gandird culdvulon mehal VN level 9D kg

th) Inaddion, yell wa werecondaced Uae very high

NGONg ha án 199,

Pests and dscussien

5 withan aterage ye 37% higher shan havo te aponss

‘hsck Nipponhae Cable 1) In conta, the paternal ines

weather Heterobelioss was observed in panicle weight and

saw welt

‘hte vl) apnea hd TR

“THURS also high yes, which were 17% igherthan ato he aois heck Honk in 2) est (Table 2 The fost ese as tht ofthe japonica vary om average ‘Under high eondons te yield of THRS as ex

‘sof the paternal nda ine Ukel 8 wa lost te same 2s that of te japonica wares

lunge pnices, an le snk sz ere a ring heading These yb ao exh strong zing resins and le

‘hon god characterises fest Table of oth japonica and indica are

Inheritance of fertility restoration of WA cytoplasm

in sodic-tolerant rice hybrids

Anuar, Sr tare Pesan and CHA ịpghorer

ly hetcochzedceEHIN(ctzofendếgzrue fea deo ste ale Fomsrg sate let rs, Tese sete sole eres ver ssenes soe sls wih P4G.2 End 025 mle ewe sui fe etc eer ne ela ae Sl The ire RSEP2S\ sở hoa wh te Perea lear het Sete de óc

‘als 94 2.0 an 025 gS me ean mt rw afte cron orale prs ol he le "lre ns sued Polen fly poceage us wet uo td at as een es

‘are ig elon th (1) cdetseung atom alte cessed vl 15 eel sane cesar ares, nea srg ute mers Te seen let Sr

Farts nol toga sma eater args Sede ta esas a arg ei, ale

‘Se nes ae sor cme te laine Soe Wks ree, ah Be fe ed

Sve tar ree ys,

code loneagetnohle MettDie se fag

enmamiyisheniddleckiaidisiolyLAngrl 3,

D00 200090990000 091010010196

‘Mend coi sin Seelam yd fect bee Tum ming bth act ose el) a

Spc mht cal comlonet Be soy le

‘stout tronty, Sule sl-lean miseries

tei inte Fon mort pheremenn yh he

‘tcc the yi vl depen Tere sds

‘teen's for ale est ns

Materatsand metho

‘ttl of 26 male ste ines representing diverse eytopas-

‘nica tery sours, suchas wild abot, O porens

‘and EC 0.25 dS m" during the 1998 wet season, Sodicitytol-

‘ace was sored or phenogpic sxepuhilt (PACP)

thy) wang 30-9 scl, with bing the mos soscepiBle r=

‘conn tems of poor germination seeding vigor lens

selested ase om hgh pen an spikelet eriyfrom Tà teseacssreened under sisi wit pf1.2anJECO2S Plans Other unstained or paral sain shrivel or ound pollen types were considered a sri, orca ybrid con

Reallsandđsasson Anon the CMS and maine inesxơecrei, IRS80B5A, IRS2SD9A, IRG670TA 10 perenis eyo) IRS

‘heir respective maintainer ines were found o be moderately

tolerant i all ve sages—germination,scelg,lesng,

‘lowering and haves att) The phenolypie accept

soxie-olerant beck, TRY I, There was no significant die

fc betvce malestnileinesand lrespestive mailer anc hans maintainer ne, cer ineating no eye Ine iueneon ster sseetbity or resisance Table P Males IR6TOTA (Operon cytapas) ands mai [MSA (MS 577A sytoplasn) and is msinaier ine were otra of say

‘Othe 120 testersses IRSEOZSA and Pasa SA with ois with pH19.2 ang ECO JS ony the ive ost prom

‘Sing F hybeid sombintons wih hgh ys under soy

‘eareaton forthe restoration of Wa cytoplasm for live

"ero yb td wel fra fre serie Tale 2,

‘atl Serena feet epee mala tr nes ad he maine

“hal 2 Pola rphokt te sai ete om cosas of OMS hee A yap wt

indicating a piste dominanepistass involving wo genes

‘Several workers have reported that eit sestration of WA

theta genes, one i stronger than the oe fr fet reto-

nic bride, pat fom ening wlan ete

‘conversion of ale see ies inte bckgrean of soe=

Genetic analysis of temperature-sensitive genic

male sterility in rice

Amar, Ser care Ragen end CHA ịpgheer

tse wre rade btien i TNS rs 1024S poten ae spoke ly ne hemp fanart crac goed wo sept gee Pal Sly ne F ee ar (25°C 5 anC10 Pet Te F.shasconplty ral 26 "Crige, MeF seve 0 eT See Compe seo 2 ete nacre fet ot a anes ner hi eno mre ge Inge 008 at aaa, a sees rae settee segeason pt unde ie et

oe perce we eee ar spec

eteur TVS an ee es ue roe The eit parang Fun on Ot

hg engerae as cade ral

Thef sagogied a sgt ra SRO Sele (TNS eto unser hgh ener (2288 Cay ard > 256°C re TGS ak ety ong

CEGMS) ini led othe development ot esimple and gh llcen tele hybrid Deeds iret sources

{hermosensiive genie male werilty (TEMS) have heen d=

"eloped in varios counties Chia, Japan nda and te

marginal, TMS ines grown vo high temperature he, 230

“C lạv»34*C ng become completly ale sti Whe

‘ue senstvetosuch empertres daring sages 01 of the

cle developmental pase (1624 before dig) The

doesnt regi any lar restorers since 95% ofthe vars

iesresore dhe eri of the, Futhermorethe cytoplasm

‘sf potential genetic ulneaBiliy The PGMS in Nongen Se

nde derivative ines was fod be governed by ai of

1989), RSPTS (Yang etal 1990), 89-1 (Noria PL 12)

(Maryams stl 1991, and $A 2 (A 1996 A et 1995)

vas contol ya single eesive gene Informatio on ite

tions (FMS non-TGMS) with ferent TGR soses

Meteralsand methods

Directorate of Rice Research, Hyderabad, India were sed (©

study aliens, Croeses were ad by eating dese

Toto 240 before healing daring November and Devers

when ihe temperate at Tuehiapall da, Wee about 2)

tem,

ing completely pollen sterile and SMS an JP becoming and IC10 was elatvely lower than that of SMS and JPs4 The

Fs were aled ring the wt season separ ows Like

se the F, generation of the eo crosses Wa used under

‘May, Pollen fertility was examined hy smeaeing anthers of five

spikelets wih 16 IKI stan under aight roscoe spitelet eiy a ied er plan averages ov 10 pans) having Ape and My when ihe empetatues wot bigh 2

^Cduy26 “Chigh) olen erilty was se or eh i

Pesutsand discussion

‘nal for poleo and spkeltfeility under hieb-temperatre

‘ios (Table Fr hid costs ie showed 480

‘he character fe governed by to separate gene Since IDDS aries the salle Rey 1997), SMS mostcay afer

¬

oe len Điện Fee Sine teects omy

nt 2 amt aa ot at

thor ndcating dat Ps cares a diferent alle Pollen se

high-temperature regimes (38 °C day/26 °C night tempera~

tures) during Api 1998s Tiachrpll The hetifcaton

‘vith verse source for developing twolne se hyhöäc

The eight most promising Fy Rael om yield gedor-

Iiphtempertire were stdod by analyzing pollen fer

The h-suareestrevald thatthe Fsepreation ited wall

the TOMS tai was governed by a single gene following a

‘ondpeniinheritane pater sinlar teacher ndings (S00

“This confirmed this, n most TOMS sours ted the ei

Is governed by singe recessive gee,

Ferences

Tne tát ine (Once ae) PD heen Ag

pie fry Fad F, aos om

Nites

Complexity of inheritance of thermosensitive genic

male sterility in rice

Re Lense andy

Tremoserste gee wale ely (CS) ne ea a or eoeg ees, eel on

‘ane, ecatisconpj ered "ons TGUS neUPROB ATOMS wth sree ee es sieaieiares he te Enerbeidoztna2tef 5p agaBannđTo 9g BETCre gta tne etouvk ni TE enn Cea sides sowed ut rt a be roopene tees, one) anddgane 15°15 enmgton aos coi ema a2 fr’ pny ei eon)

sole utes sore othe dese egestons wore shed in 360 P10 et raider ect, tints gat smc cen neon urge ly wa bane Nae ee srg {pres conenescongn ae es etl the sovesring sora gre remem ety

‘ere chr arte napster ere was 17 21 bres af sxc rd ee

(ret eves ncarlon, te cent) oe ES eras stem agrees as

“Taemosestive gone male seity (TMS) sonia

twolfordeveloping two-line rice yids Thi sstmns ore

‘rid send prodictin an roar cic of mal parent fo

ahancing yell potential Because he maintaier a estore

nce and thes indepenent genes mt, Yang el 1992) 1m,

(Mariya el 1991) ad ta, Borkakatl and Vian

repo, Recently, anew TEMS source, UPRIOS: AUTOM,

tiene nour hybrid breeding program (Pandey c1 dì

Ive revealed more complicit inheritance pre ofthe

Intertance pattem of TMSin UPR 95-1 40TGMS

“Sogrogation for afferent fortity types

‘with known feriliy-steriliy ransformation behavior under

tale feity were grown lathe 1998 wer season (WS) at

BR.S5-2872'Cofmean Id hemounsve tempest 3°

‘ere, Rest evestes thee kindof rreanion when IfE

ofthe spite fet eetevion was adopted to ascea Fee-

Resolution of steity sogragation

Four pairs of TGMS nesrogeni ines (NIL) in lege

nese backgrounds were developed and pa-crosed The ác

‘ach plant were removed fo promot latter formation and

hcaing eileen dts mst he sondton fiero

sterile plans inthe F, populations of ơnsees DFTRI 9%,

16:39, respectively These ited the expec ratio of

B.S inating monogenic segregation in Ferlito fio he expected oof 1, confing nnogent inher

To sonplee xenlly ident tht ofthe ema pert

vi ety <8) an ys ste (5 ety >but soled into I2FPSICS an 2: IPS-CS, rerpesively Table 1) This suggested monogene segregation in ely and te

‘ly, digo sepegain orient clases (San CS) fio of {SFIS nthe Fe am 3P ln hệ TC, te cos Resstsindcsed genie soreation fo frit and tery, and igeni epepaon forte tw classes of ky in th

‘ross (Table tint lees nthe F and TEs two roses seeped the rato of ISRICS in the Fz and 3PICS in TC, wheres one Ulgeie nature of segregation fo Fert and stent

{Sts tom Fer ln Grup fom PS pu a

oe F polation DatinTble? towed kf segregation nthe prog Fomorygur nie of the pans However th popes {Group I cạeguel 3 epeeatne' selerecheedug ropes ad wer doi eben erie possi population of tee cross (abe Ian at ia on {lly agent fcorandaat det theenionment The

‘ele seresing progenies, Varin nthe seegxton patente pupesuldbe enn etait 55012MUROMALUR 9515 y nho wih doe tnongous meee gener were le sete Tee he

“hole Supt pate pln raya and etre 1) naan of he teen

for UPTRI 9S-14OTGMSIOPRI 95-141 ad PP

mint gone formate ey was ered The PS plate

inthe, posessed only asinge jo but wesker dominant

‘ther near homeypowsrcesve gee, Therefore nthe Fy

{steration the Group Ia Group | progenies earegatd 2

ecto ferent gees on male fry

Ie way astm tht the TOMS tro UP 9%-1401GMS,

the Female ara, was unde the conta of thos paso =

enone ans it he gene fost a> for male ste Pity and hecoresponing genes TT, > or male

DS LONI and UPẠI95-130TOMAVUPRI S he peo

‘ype sui was completely sera could he dings {fom other lagleclees PS Mand) st 2861/2825 Co

"suttty were Indsint from eah other hs empersre since Both wee completely stl but hey were đit ai pletely sere In this way, fret genotypes in diferent roses ere MeNUie0 an heir genotypic a gi eet mm [38612325 Caf Is-ITE the gene Tha nô iuecean