Virus structure tutorial STEM

Virus Structure• Virus capsids function in: – Packaging and protecting nucleic acid – Host cell recognition • Protein on coat or envelope “feels” or “recognizes” host cell receptors – G

Introduction to Virus Structure

Tutorial

Jonathan King, Peter Weigele, Greg Pintilie, David Gossard

(MIT) v.November, 2008

• Protective Shell - Capsid

– Made of many identical protein

Virus Structure

• Virus capsids function in:

– Packaging and protecting nucleic acid

– Host cell recognition

• Protein on coat or envelope “feels” or “recognizes” host cell receptors

– Genomic material delivery

• Enveloped: cell fusion event

• Non-enveloped: more complex strategies &

specialized structures

Electron Microscopy

Mitra, K & Frank, J., 2006 Ribosome dynamics: insights from atomic structure modeling into cryo-electron

microscopy maps Annual review of biophysics and biomolecular structure, 35, 299-317.

• In 1953, Crick & Watson proposed …

principles of virus structure

– Key insight:

• Limited volume of virion capsid => nucleic acid sufficient to code for only a few sorts of proteins of limited size

– Conclusion:

• Identical subunits in identical environments

• Icosahedral, dodecahedral symmetry

X-ray Crystallography of Viruses

• Symmetry of protein shells makes them uniquely well-suited to crystallographic methods

• Viruses are the largest assemblies of biological macromolecules whose structures have been

determined at high resolution

Similarity to Buckminster Fuller’s

Geodesic Domes

Caspar and Klug’s Icosahedral

shell

But …

• Clear evolutionary pressure to make larger capsid

– Using larger subunits helps very little

– Using more subunits helps a lot

• Not possible to form icosahedral shell (of identical units in identical environments) with more than 60 subunits

• Viruses with more than 60 subunits were observed

• Question :

– How can >60 subunits form an icosahedral shell?

– Will any number of subunits work?

– If so, how would they be organized?

• In 1962, Caspar & Klug proposed the

theory of “quasi-equivalence”

– Not all protein subunits are equivalent

• “Identical” subunits in slightly different environments

– Only certain numbers of subunits will can be packed into closed regular lattice.

Caspar & Klug, Cold Spring Harbor, 1962

– Shift from T1 to T4 packing

=> 8-fold increase in volume

Spherical viruses have icosahedral symmetry

Homunculattice

HK97 Asymmetric Unit

Herpes Simplex Virus at 8.5 Å resolution

• Infection depends on spike proteins projecting from capsid membrane called “Hemagglutinin (HA)”

• These bind sugar molecules on cell surface

• Much of the difference between Hong Kong flu, Swine flu, Bird flu, and other strains, is in the amino acid sequence and conformation of the HA protein

• These differences control what host cell types the virus can infect

• Immunization against flu involves your immune system synthesizing antibody proteins that bind the HA protein

Influenza virus

entry of influenzainto cell

composition of virus

a pH induced, conformationally controlled trigger

for membrane fusion

backbone is structured

disordered loop

Qiao et al Membrane Fusion Activity of Influenza Hemagglutinin The Journal of Cell Biology, Volume 141, 1998

Influenza Hemagglutinin

• The HA spikes extend like a spring during infection

Trimer Structure

• Long alpha helices form

coiled coil structure

• In mature trimers of HA0,

each monomer is cleaved

into HA1 and HA2

Evolution of dsDNA viruses

• All known viruses, whether infecting

bacteria or humans, may have evolved from

a single common ancestor, relatively early

in the evolution of organisms.

Common steps in the assembly of all dsDNA

viruses

• Unique portal ring at one Vertex

• Scaffolding proteins

• Procapsid assembled empty of DNA

• DNA pumped into procapsid through portal ring

• DNA moves back through portal to enter

cell

P22 Pathway

Herpes viruses also have a portal protein

Herpes portal (UL6) tagged with gold-bead labeled antibodiesvisualized by negative stain electron microscopy

portalcomplex

Bill Newcomb and Jay Brown, University of Virginia

Trus BL, Cheng N, Newcomb WW, Homa FL, Brown JC, Steven AC

Structure and polymorphism of the UL6 portal protein of herpes

Cryo-EM structure of purified Herpes portal protein