Development of 68ga generator at ANSTO

ANSTO Publications Online http://apo.ansto.gov.au Bad Berka, Germany June 23-26, 2011 P-023 Van So Le*, Michael Izard, Paul Pellegrini and Myint Zaw ANSTO LifeSciences, Australian Nuc

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Conference Presentation

ANSTO Publications Online

http://apo.ansto.gov.au

Bad Berka, Germany June 23-26, 2011

P-023

Van So Le*, Michael Izard, Paul Pellegrini and Myint Zaw

ANSTO LifeSciences, Australian Nuclear Science and Technology Organisation

Locked Bag 2001 Kirawee DC, NSW, 2232, Australia

* E-mail: slv@ansto.gov.au

A 68Ge/68Ga generator combined with an automated 68Ga eluate purification unit was developed to produce 68Ga solution suitable for labelling peptide ligands for PET radiopharmaceutical applications The sorbent of a Ti-Zr ceramic structure [1] was used as a generator column packing material The SEM picture of micro- and meso-porosity of these materials is shown in Figure 1a Its X-ray diffraction presents a tetragonal nano-crystalline structure

The adsorption capacity for Ge4+ ions is approximately 120 mg Ge per gram sorbent

in 0.1 M HCl solution The distribution coefficient Kd > 10000 mL /g for carrier-free

68

Ge4+ ions and 2 mL/g for 68Ga3+ were evaluated in 0.1 M HCl solution A 1.0 g weight sorbent column was used for immobilizing the parent nuclide 68Ge and 68Ga was eluted from the column with 3- 4 mL 0.05 – 0.1 M HCl solution The 68Ge breakthrough in 68Ga eluate was around 10-3 % for a generator of 18 mCi 68Ge activity

Figure 1 (a) SEM picture of Ti-Zr ceramic sorbent used for loading 68Ge/68Ga generator column; (b) PET imaging of the 68Ge/68Ga generator column after 300 68Ga elution cycles

The PET imaging picture of the 68Ga generator column after 300 elution cycles (Figure 1b) showed no significant spreading and drift of 68Ge zone along the column

68

Ga eluate of around 5 mL volume in 0.1 M HCl solution was purified on a small cation exchanger column with an aqueous alcohol solution mixture of hydrochloric

b

a

acid, ascorbic acids and halide salts An alkali solution was used for elution of 68Ga from the ion exchange resin column to obtain a purified 68Ga solution which is conditioned with acidic solution to obtain a final 68Ga product of pH=3-4 in 0.75 mL 0.5 M NaCl or 0.5 M sodium acetate solution The metallic contamination <20 nano grams per mL were found in this product solution The organic solvent free 68Ga solution product of acidity suitable for coordination chemistry based labelling of the peptide ligands was successfully used for preparation of DOTATATE and DOTATOC PET radiopharmaceuticals

The process of 68Ga elution from the generator followed by 68Ga eluate purification was performed using a low-cost automation bench-top system [2] This system is designed (Fig 2) based on the timing sequence of seven processing steps without feedback control The variable flow rate of eluents used for elution/purification in this system also ensure the optimisation of operating times with respect to different adsorption/ desorption kinetics of 68Ga ion species, which is controlled by the sorbent and ion exchange resin used in the generator and purification columns

Figure 1: 68Ga generator (housed in the lead container in the lower part of the system) and processing unit with the eluents for 68Ga eluate purification (in the upper part) together with a control unit at the bottom right

References

1 Van So Le, Sorbent material, Patent, PCT/AU2011/000245

2 Van So Le, 68Gallium purification, Patent, PCT/AU2011/000244

How to cite this article:

Van So Le, Michael Izard, Paul Pellegrini and Myint Zaw, Development of 68 Ga Generator at ANSTO ANSTO Publications Online

http://apo.ansto.gov.au/dspace/handle/10238/3701

http://apo.ansto.gov.au/dspace/bitstream/10238/3701/1/%281b%29-Le%20VS-%20Development%20of%2068Ga%20generator%20%20at%20ANSTO.pdf