This human protein was produced in yeast mitochondria to probe IRP1 folding in this organelle where iron–sulfur synthesis originates.. Abbreviations hAco2, human mitochondrial aconitase;
Trang 1depend on its subcellular localization
Alain Martelli1, Be´ne´dicte Salin2, Camille Dycke1, Mathilde Louwagie3, Jean-Pierre Andrieu4,
Pierre Richaud5and Jean-Marc Moulis1
1 Laboratoire de Biophysique Mole´culaire et Cellulaire (UMR-CNRS 5090 ⁄ Universite´ Joseph Fourier), CEA-Grenoble, France
2 Institut de Biochimie et Ge´ne´tique Cellulaires du Centre National de la Recherche Scientifique (UMR-CNRS 5095⁄ Universite´ Victor Segalen), Bordeaux, France
3 Laboratoire de Chimie des Prote´ines (ERIT-M 02–01), CEA-Grenoble, France
4 Institut de Biologie Structurale JP Ebel (CEA ⁄ CNRS ⁄ Universite´ Joseph Fourier), Grenoble, France
5 Laboratoire des Echanges Membranaires et Signalization (UMR-CNRS 6191 ⁄ Aix-Marseille II), Saint Paul les Durance, France
Aconitases are found in a wide range of living
organ-isms, from bacteria to higher eukaryotes [1] They are
metalloproteins containing a [4Fe)4S] cluster that
binds citrate or isocitrate and acts as a Lewis acid to
isomerize these substrates Aconitases catalyse one
reaction of the citric acid cycle and they participate in
supplying the precursors of essential nutrients such as glutamate In eukaryotic cells, proteins with aconitase activity can be found in different compartments, inclu-ding mitochondria, for the enzymes of the citric acid cycle, and the cytosol [2] Deletion of ACO1, YLR304C, the gene encoding mitochondrial aconitase,
Keywords
aggregation; iron–sulfur cluster biogenesis;
a-ketoglutarate dehydrogenase;
Saccharomyces cerevisiae; translocation
Correspondence
J.-M Moulis, CEA-Grenoble, DRDC ⁄ BMC,
17 rue des Martyrs, 38054 Grenoble Cedex
9, France
Tel: +33 4 38 78 56 23
E-mail: jean-marc.moulis@cea.fr
(Received 24 October 2006, revised
4 December 2006, accepted 19 December
2006)
doi:10.1111/j.1742-4658.2007.05657.x
Aconitases are iron–sulfur hydrolyases catalysing the interconversion of cit-rate and isocitcit-rate in a wide variety of organisms Eukaryotic aconitases have been assigned additional roles, as in the case of the metazoan dual activity cytosolic aconitase–iron regulatory protein 1 (IRP1) This human protein was produced in yeast mitochondria to probe IRP1 folding in this organelle where iron–sulfur synthesis originates The behaviour of human IRP1 was compared with that of genuine mitochondrial (yeast or human) aconitases All enzymes were functional in yeast mitochondria, but IRP1 was found to form dense particles as detected by electron microscopy MS analysis of purified inclusion bodies evidenced the presence of human IRP1 and a-ketoglutarate dehydrogenase complex component 1 (KGD1), one of the subunits of a-ketoglutarate dehydrogenase KGD1 triggered formation
of the mitochondrial aggregates, because the latter were absent in a KGD1– mutant, but it did not efficiently do so in the cytosol Despite the iron-binding capacity of IRP1 and the readily synthesis of iron–sulfur clus-ters in mitochondria, the dense particles were not iron-rich, as indicated by elemental analysis of purified mitochondria The data show that proper folding of dual activity IRP1-cytosolic aconitase is deficient in mitochon-dria, in contrast to genuine mitochondrial aconitases Furthermore, effi-cient clearance of the aggregated IRP1–KGD1 complex does not occur in the organelle, which emphasizes the role of molecular interactions in deter-mining the fate of IRP1 Thus, proper folding of human IRP1 strongly depends on its cellular environment, in contrast to other members of the aconitase family
Abbreviations
hAco2, human mitochondrial aconitase; (h)IRP, (human) iron regulatory protein(s); KGD1, a-ketoglutarate dehydrogenase complex
component 1; m- and c-, location for protein production, mitochondria and cytosol, respectively; yAco1, yeast mitochondrial aconitase.
Trang 2confers glutamate auxotrophy on Saccharomyces
cere-visiae Mutant cells cannot grow on nonfermentable
substrates [3] More recently, mitochondrial aconitase
has been assigned new roles, including the stabilization
of mitochondrial DNA [4]
In mammalian cells, two aconitase paralogs are
pre-sent in the cytosol: iron regulatory proteins 1 and 2
(IRP1 and IRP2) IRP1 and IRP2 are important
regu-lators of cellular iron traffic in metazoans [2] They
are trans-acting elements that bind to the mRNA of
key proteins involved in the use, storage and transport
of iron in the cell; this translational regulation relies
on the interaction of IRP with specific RNA sites
called iron responsive elements, which are found in the
untranslated sequences of the transcripts Whereas
IRP2 is not known to display aconitase activity, IRP1
can assemble a [4Fe)4S] cluster to become a functional
cytosolic aconitase while loosing its mRNA-binding
activity The balance of IRP1 between its
iron–sulfur-containing form and apoprotein is the main process
that controls IRP1 activity as a regulator of cellular
iron management [2] The importance of the aconitase
activity of IRP1 in the cytosol of mammalian cells
remains to be fully assessed, although roles in the
pro-duction of the precursors of fatty acid b-oxidation or
in the resistance to oxidative stress, by providing the
substrate of isocitrate dehydrogenase and favouring
cytosolic NADPH production, may be proposed
The similarities between mitochondrial and cytosolic
aconitases of animal cells include the ability to bind
[4Fe)4S] clusters Iron–sulfur cluster biosynthesis has
recently been the topic of intense interest, particularly
in S cerevisiae An extensive set of proteins
participa-ting in different stages of the process has been
discov-ered More than 10 such proteins have been found in
mitochondria where iron–sulfur cluster synthesis is
believed to begin Other proteins are also needed in
the cytosol for extra-mitochondrial iron–sulfur proteins
[5] Metazoan aconitases are of interest in this respect
because they are two homologous proteins that
specif-ically locate to distinct subcellular compartments and
each assembles an iron–sulfur cluster Furthermore,
accurate structural data are available for prototypes of
both proteins, with models for bovine mitochondrial
[6] and human cytoplasmic [7] aconitases They show
that these proteins are structurally very similar, and
suggest similar requirements for assembly of the iron–
sulfur units
It is of interest therefore to know whether the
fold-ing of these two proteins around their iron–sulfur
clus-ter occurs similarly in mitochondria and in the cytosol
To address this question, human IRP1, yeast
mitoch-ondrial aconitase (yAco1), and human mitochmitoch-ondrial
aconitase (hAco1) were produced either in the cytosol
or in the mitochondria of S cerevisiae Whereas tar-geting of the different aconitases occurred as expected, ultrastructural analysis combined with proteomic methods revealed unexpected protein–protein interac-tions interfering with efficient mitochondrial protein turnover in the case of human cytosolic aconitase These results emphasize specific features of human IRP1 among aconitases which may be associated with its exceptional regulatory function among proteins of the aconitase family
Results
Human IRP1 displays aconitase activity in yeast mitochondria
Saccharomyces cerevisiae cells harbouring the different plasmids were grown with glucose as the carbon source In the case of the YEpLmitIRP1 plasmid, the yeast aconitase presequence was fused to the complete coding sequence of human IRP1 as a way of translo-cating the protein to yeast mitochondria The aconitase activity of all proteins addressed to mitochondria was probed by complementation of the glutamate auxotro-phy exhibited by the ACO1-depleted S cerevisiae FYF4-A1 aco1 strain, i.e cells in which the gene enco-ding mitochondrial aconitase was deleted There was
no growth of cells containing the empty vector on a minimal medium depleted of glutamate, as expected from the aco1 genotype (Fig 1, first row) By contrast, all aconitases directed to yeast mitochondria rescued the growth deficiency in the absence of glutamate (Fig 1) Furthermore, human IRP1 addressed to mito-chondria was hardly less efficient than genuine mitoch-ondrial aconitases (from yeast or human, targeted with its own presequence in the latter case) This indicates that aconitase activity is present in all strains, inde-pendent of the origin of the cloned cDNA and whether the enzymes are naturally located in mitochondria However, the data do not demonstrate the proper tar-geting of the proteins to mitochondria Therefore, ultrastructural analysis of these yeast strains was carried out
Immunocytochemical and ultrastructural analysis
of mitochondria-targeted IRP1 Saccharomyces cerevisiae W303-1B cells producing m-hIRP1 were analysed using immunogold electron microscopy First, use of the preimmune serum as a control revealed that there was no nonspecific staining of these cells (Fig 2A) However, the presence
Trang 3of electron-dense particles was noted in mitochondria
(Fig 2) These particles appeared globular in shape,
with an ovoid contour and diameters of 100–500 nm
They will be referred to as inclusion bodies, aggregates
or dense particles in the following
The use of polyclonal antibodies recognizing hIRP1
(Fig 2B,C) resulted in heavy and uniform labelling of
all inclusion bodies, which clearly indicated the
associ-ation of hIRP1 with these particles The remaining
vol-ume of mitochondria was only faintly labelled with
little evidence of cristae Outside the mitochondria,
hIRP1 was hardly detected This demonstrates efficient
mitochondrial addressing of hIRP1 fused to the yeast
mitochondrial aconitase presequence With raffinose, a
nonexclusively fermentable substrate, dense particles
were even more frequent in the mitochondria than
when glucose was used as a carbon source (Fig 2C)
Indeed, > 16% of mitochondria contained inclusion
bodies in 25 cells grown on raffinose, whereas this
pro-portion decreased to < 7 in 20 cells grown on glucose
Mitochondrial inclusion bodies were not detected in
cells producing c-hIRP1, i.e in which the protein
remained in the cytosol However, aggregates could occasionally be visualized in the cytosol of these cells, whether glucose or raffinose was used as a carbon source (not shown): a single dense cytosolic particle was counted in 2 of 150 cells In these rare cases the aggregates were systematically and uniformly immuno-labelled, accounting for the presence of hIRP1
To ensure that electron-dense particle formation in mitochondria was not due to a general mitochondrial defect induced by plasmid-driven aconitase synthesis and that it was specific to hIRP1 production, we ana-lysed the same yeast strain expressing either the human
or the yeast mitochondrial aconitase genes (YEp-Laco2P and YEpLScmaco plasmids, respectively) Each protein was addressed to yeast mitochondria under the dependence of its own presequence in other-wise identical expression conditions Yeast cells, grown
in glucose at 30C, were examined for ultrastructure using transmission electron microscopy No electron-dense mitochondrial materials were observed with human or yeast mitochondrial aconitases (Fig 3), indi-cating that the inclusion bodies (Fig 2) were specific
Fig 1 Complementation assay of the FYF4-A1 aco1 strain by different aconitases FYF4-A1 aco1::ura3 was transformed with plasmids enco-ding the proteins indicated on the left No insert was cloned in YEpPL1 + (first row) and m-hIRP1, m-yAco1, and m-hAco2 were produced from YEpLmitIRP1, YEpScmaco, and YEpLaco2P, respectively Decreasing numbers of cells (5 · 10 4
, 5 · 10 3
, 2.5 · 10 3
, 5 · 10 2
, 2.5 · 10 2
,
50 left to right) were deposited on Petri dishes containing a minimal medium with (left) or without (right) glutamate.
Fig 2 Immunocytochemical analysis of S cerevisiae producing human IRP1 in mitochondria W303-1B cells producing m-hIRP1 were ana-lysed by immunocytochemistry m, mitochondrion; v, vacuole; bars ¼ 200 nm (A) Cells probed with the preimmune serum (B) Cells grown
on glucose and probed with polyclonal antibodies raised against hIRP1 (C) Cells grown on raffinose and probed with anti-hIRP1 sera.
Trang 4to the heterologous production of
mitochondrion-addressed hIRP1 Immunogold electron microscopy of
W303-1B producing m-yAco1 and polyclonal
antibod-ies against yeast mitochondrial aconitase revealed
labelling but no evidence of mitochondrial segregation
of aconitase-rich particles (Fig 4) Ultrastructural
ana-lysis of W303-1B cells producing c-human
mitochond-rial aconitase (hAco2) showed no aggregate formation
in the examined sections, suggesting that the rarely
observed aggregates containing c-hIRP1 in W303-1B
were also specific to hIRP1 production in this
com-partment (not shown)
Purification of inclusion bodies
To further examine mitochondrial inclusion body
com-position, purified mitochondria of W303-1B cells
pro-ducing m-hIRP1 were sonically disrupted The lysate was applied to a discontinuous sucrose gradient to sep-arate particulate materials from bulk membrane and soluble proteins Fractions of the gradient were ana-lysed on SDS⁄ PAGE with silver staining (Fig 5A) With reference to the recombinant protein (lane 1), lit-tle staining in the size region of hIRP1 was observed
in the soluble fractions of mitochondria (lanes 2–4) In contrast, protein fractions separated with higher con-centrations of sucrose, up to 80%, showed heavy stain-ing in the correspondstain-ing range Immunodetection of hIRP1 using western blotting (Fig 5B) confirmed the presence of the protein in fractions 5–10, whereas only
a small amount of IRP1 was visualized in the first fractions (Fig 5B, lanes 2–4) The gel in Fig 5A shows many other proteins in each fraction, including those rich in IRP1 To gain insight into the possibility
of coprecipitation of proteins with IRP1 and to assess possible nonspecific binding, attempts at dissolving a sample of fraction 10 were carried out After dilution for 10 min in ice-cold Tris⁄ Cl (10 mm pH 7.4), samples were centrifuged to pellet the particulate material which was again analysed on SDS⁄ PAGE with silver staining (Fig 5C) Most of the bands in Fig 5A, lane 11 (fraction 10) were still present (Fig 5C, lane 1), but addition of 1% Triton X-100 to the buffer eliminated many of the protein bands (Fig 5C, lane 2)
In this case, two proteins appeared to be the main components of the isolated aggregate: the major one was m-hIRP1, as further confirmed by immunoblotting (not shown), and the second was a larger protein with
a molecular mass around 110 kDa However, more extensive washing and dilution of the aggregates with
10 mm Tris buffer, pH 8.0, overnight at 4C, dis-solved part of their content, including the above two proteins It thus appears that the proteins trapped in the inclusion bodies are not irreversibly denaturated, and it might be envisioned that some exchange between dense particles and the mitochondrial matrix
is possible in cells
N-Terminal sequencing of mitochondrion-directed hIRP1
To address human IRP1 to yeast mitochondria, the presequence of yeast mitochondrial aconitase was fused
to the N-terminus of hIRP1 This sequence allowed the correct targeting of m-hIRP1 to the organelle, as observed using immunogold electron microscopy (Fig 2B,C), but the actual processing of the protein in mitochondria was not accessible using microscopic experiments Mitochondria-targeted hIRP1 is expected
to be translocated, presumably as the apo-protein, the
Fig 4 Immunocytochemical analysis of S cerevisiae producing
yeast mitochondrial aconitase W303-1B cells producing m-yAco1
were probed with polyclonal antibodies raised against yeast
mito-chondrial aconitase m, mitochondrion; v, vacuole; bar ¼ 200 nm.
Fig 3 Ultrastructure analysis of S cerevisiae producing
mitochond-rial aconitases W303-1B producing (A) m-yAco1 (bar ¼ 500 nm) or
(B) m-hAco2 (bar ¼ 200 nm) plasmids were grown on glucose and
examined m, mitochondrion; N, nucleus.
Trang 5presequence is then cleaved using specialized peptidases,
and the protein is folded inside mitochondria To check
that hIRP1 was properly processed in yeast
mitochon-dria, aggregates were prepared from fractions obtained
at a 60% sucrose gradient (Fig 5A) Proteins were
separated by SDS⁄ PAGE and transferred to a
poly(vinylidene fluoride) membrane The band
corres-ponding to m-hIRP1 was cut and its N-terminal
sequence was determined From this single band, two
N-terminal sequences were obtained They correspond
to cleavage after the 15th (glycine) or 16th (leucine)
amino acid, in agreement with the recognition sequence
of the mitochondrial matrix processing peptidase, one
and two amino acids after an arginine [8] Cleavage after
leucine 16 has been recently identified for processed
yeast mitochondrial aconitase [9]
Identification of the aggregated proteins using MS
The two main proteins of the mitochondrial aggregates formed by production of mitochondrion-addressed hIRP1 in yeast cells (Fig 5C) were further considered One is hIRP1 addressed to mitochondria and the other one is unknown The protein bands separated from purified aggregates on SDS⁄ PAGE were cut and iden-tified using MS The results indicated the presence of hIRP1, as expected, in band 1 of Fig 5C The unknown protein in band 2, with an apparent molecu-lar mass of 110 kDa, was found to be mitochondrial 2-oxoglutarate dehydrogenase KGD1p, the E1 compo-nent of the yeast a-ketoglutarate dehydrogenase com-plex This subunit has a calculated relative molecular
A
B
C
Fig 5 Isolation and analysis of aggregates
from purified mitochondria (A) Sucrose
gra-dient of sonically disrupted mitochondria
purified from strain W303-1B producing
m-hIRP1 Twenty microlitres of each
fraction were analysed on SDS ⁄ PAGE (8%
acrylamide) and the gel was silver stained.
Lane 1, recombinant human IRP1; lanes 2–
11, fractions 1–10 of the sucrose gradient.
(B) Western blot of the same fractions.
Lane 1, recombinant human IRP1; lanes 2–
11, fractions 1–10 of the sucrose gradient.
(C) Fraction 10 was diluted in Tris buffer
(lane 1) or Tris buffer containing 1%
Tri-ton X-100 (lane 2) at 4 C, centrifuged, and
the pellet was analysed by SDS⁄ PAGE.
Trang 6mass of 114 345 (for the unprocessed protein) and
data for 25 tryptic peptides provided 28% of sequence
coverage
Role of KGD1p in the formation of mitochondrial
aggregates containing hIRP1
It was then of interest to know whether KGD1p was a
bystander protein which was nonspecifically trapped
by hIRP1 precipitation in yeast mitochondria or if it
was an active participant to the formation of the
inclu-sion bodies To answer this question, a mutant strain
in which the KGD1 gene is inactivated was used as a
host for hIRP1 synthesis in yeast mitochondria
Pro-duction of recombinant hIRP1 was checked by western
blot in this mutant (Fig 6A, lane 2) and in the control
strain (Fig 6A, lane 3) Ultrastructural analysis of
the KGD1– cells producing m-hIRP1 did not evidence
any dense materials inside the mitochondria (Fig 6B),
in contrast to KGD1+ cells (Fig 2B,C) Therefore,
KGD1p is actively involved in hIRP1 precipitation
inside yeast mitochondria, and it is not a passive
pro-tein carried along by formation of IRP1 aggregates
Elemental analysis of yeast mitochondria
Although iron homeostasis in yeast is transcriptionally
regulated, the aconitase activity of mammalian IRP1
has been shown to be sensitive to iron depletion [10]
Human IRP1 produced in yeast mitochondria
dis-played aconitase activity and rescued glutamate
auxo-trophy of the FYF4-A1 aco1 strain (Fig 1) Human
IRP1 is thus able to bind a [4Fe)4S] cluster in yeast
mitochondria Defects affecting genes participating in
yeast iron homeostasis have been shown to result in iron accumulation in mitochondria: this is the case for Yfh1- (yeast frataxin homolog) or Yah1-deleted strains [11] In a similar way, pathophysiological features of human mitochondrial diseases, such as X-linked side-roblastic anaemia with cerebellar ataxia, include accu-mulation of materials in mitochondria with the close association of transition metals such as iron [12] Because massive precipitation of iron-binding IRP1 may trap this metal inside mitochondria, the metal content of purified mitochondria obtained from W303-1B cells producing c- and m-hIRP1 was investigated Mitochondria purified from these cells were dried and analysed using inductively coupled plasma-atomic emission spectrometry No significant accumulation of transition metals seemed to correlate with the forma-tion of m-hIRP1 aggregates Of particular interest, iron was found in almost equal proportions (within 15%) in mitochondria of yeast cells producing c-hIRP1
or m-hIRP1, with inclusion bodies in the latter case
Discussion
In this study, the cytosolic IRP1 protein of animal cells was shown to be an efficient aconitase in yeast mito-chondria (Fig 1) Therefore, the iron–sulfur cluster assembly machinery of yeast mitochondria can build the inorganic unit of this normally cytosolic protein Furthermore, members of the extra-mitochondrial assembly line of these cofactors, the so-called cytosolic iron–sulfur assembly machinery [5], are not needed to correctly fold cytosolic aconitase⁄ IRP1 As expected from the design of the reported IRP1 expression system, immunogold labelling experiments of cells
A
B
Fig 6 KGD1p contributes to the formation
of IRP1-containing aggregates in yeast mito-chondria (A) Western blots of BY4741 extracts with IRP1 (upper) and alcohol dehy-drogenase (lower, used as a loading control) antibodies Lane 1, no plasmid-borne duction of aconitase; lane 2, m-hIRP1 pro-duction; lane 3, m-hIRP1 production in the KGD1 mutant (B) Ultrastructural analysis of BY4741:KGD1 producing m-hIRP1 Two ima-ges are shown m, mitochondrion Bars ¼
200 nm.
Trang 7producing m-hIRP1 established that a large
propor-tion of the human protein was present in yeast
mito-chondria (Fig 2) However, the surprise was that
electron-dense particles occupied a very large volume
of IRP1-containing mitochondria, whereas this protein
rarely produced aggregates in the cytosol All inclusion
bodies were uniformly labelled using anti-hIRP1 sera,
whereas limited labelling was visualized outside these
aggregates Using the same expression vector, yeast
and human mitochondrial aconitases did not show the
formation of electron-dense particles (Figs 2,3), clearly
indicating that this phenomenon is specific to human
IRP1 among proteins displaying aconitase activity
Massive accumulation of m-hIRP1, if it was
precipi-tated as aconitase in the large structures evidenced
using electron microscopy, may be expected to
corre-late with increased iron burden because this protein
has the property of binding four iron atoms as a
[4Fe)4S] cluster Elemental analysis clearly rules out
this possibility because the amount of iron present in
the mitochondria of yeast cells with m-hIRP1 is the
same as that in the mitochondria of cells producing
c-hIRP1 Human apo-IRP1 is known to precipitate
easily and to form aggregates in vitro in the presence
of the divalent metals zinc and cadmium [13], which
probably act on the 3D structure of the protein [14]
In this respect, inductively coupled plasma-atomic
emission spectrometry experiments carried out on
puri-fied mitochondria or aggregates did not indicate the
accumulation of a specific transition metal, such as
zinc or iron, which may partly explain the formation
of dense particles containing hIRP1 It is thus very
likely that hIRP1 precipitates in yeast mitochondria as
the protein devoid of metal Inclusion body formation
in yeast mitochondria is then a direct consequence of
the presence of unfolded or incorrectly folded human
IRP1 This implies that formation of inclusion bodies
competes with assembly of the [4Fe)4S] cluster in
IRP1 In addition, degradation of the accumulated
protein is not efficiently carried out
Folding and degradation of proteins in yeast
mito-chondria have been extensively delineated [15,16] Key
participants in these processes are the HSP60–HSP10
chaperone system [17] and the ATP-driven Lon
prote-ase [16] Of relevance in this study, the Lon protein
was shown to have chaperone activity and Lon– cells
displayed large mitochondrial inclusions [18] However,
the chaperones and degradation requirements appear
to be met in our experiments because deposits of
m-yAco1 and m-hAco2 were not detected (Figs 3,4)
Mitochondrial aconitase and IRP1 are strikingly
sim-ilar proteins when their structures are compared, but
additional peptides on the surface of the protein are
predicted to contribute to the specific properties of IRP1 [7] This additional material may interfere with proper HSP60 binding [17] or it may contribute to the association of additional protein material present in the mitochondrial matrix
Indeed, proteomic analysis of purified inclusion bod-ies identified two main proteins: hIRP1, as expected and observed by electron microscopy, and the KGD1 gene product, i.e the E1 subunit of the KGDH com-plex Association of metabolic enzymes in mitochon-dria is not without precedent For instance, enzymes of the citric acid cycle [19] were proposed to assemble in
a metabolon [20], and nucleoid-associated proteins were identified by cross-linkage [21]: mitochondrial aconi-tase was shown to contribute to mitochondrial DNA stability and subunits of the KGDH complex were also bound to these large structures, together with > 20 other mitochondrial proteins [22] However, the com-position of the aggregates in this study is far simpler, with merely hIRP1 and KGD1p as major components Independent experiments using proteome-wide immunocoprecitation of tagged-proteins [23,24] indica-ted that KGD1p could interact with many proteins, even with some located outside the mitochondria, which show affinity for nucleic acids, in particular RNA It is of note that one of the roles of human IRP1 in metazoan cells is to tune iron homeostasis by binding to iron responsive elements, i.e specific RNA pieces It might be that binding of KGD1p to RNA-interacting patches of IRP1, as suggested by the struc-ture of the latter protein [7], triggers the building of electron-dense particles in mitochondria Interaction of KGD1p with mitochondrial yeast or human aconitases
is clearly not as strong as with human IRP1 because
no inclusion bodies were observed in these cases For-mation of aggregates is not a general feature of the heterologous production of human aconitases in yeast mitochondria, but a particular property of human IRP1 The active participation of mitochondrial KGD1p is borne out by the lack of aggregates in a KGD1 mutant (Fig 6) and implies that association of IRP1 and KGD1p eludes Lon proteolysis The scen-ario applies inside mitochondria only Indeed, both nucleus-encoded KGD1 and plasmid-encoded IRP1 cDNA are translated in the cytosol before being imported into mitochondria, but only marginal aggre-gate formation has been observed in the yeast cytosol
It thus may be concluded that protein clearance is more efficient against IRP1–KGD1 coprecipitation in the cytosol than in mitochondria, or that the two pre-cursors do not efficiently interact
The inclusion body formation clearly evidences that human IRP1 does not share all structural and
Trang 8functional properties of mitochondrial aconitases The
yeast mitochondrion has previously been shown to be
the sole, or at least the main, centre for iron–sulfur
cluster biosynthesis This compartment should be the
optimal environment for the folding of iron–sulfur
proteins [5] and it is quite efficient with the proteins
studied here (Fig 1) However, in the case of human
cytosolic aconitase, iron–sulfur cluster assembly at the
active site of hIRP1 produced in mitochondria does
not seem to out-compete KGD1p binding to the newly
imported apo-IRP1 Therefore, proper building of the
iron–sulfur unit is not the only reaction of importance
to stabilize IRP1 as an aconitase Other factors should
play significant roles in the cellular equilibrium
between the active forms of human IRP1, as
exempli-fied by KGD1p in this study It is likely that
compo-nents of the cytosolic iron–sulfur assembly system [5]
participate in the folding around the IRP1 iron–sulfur
cluster, but protein–protein interactions involving
IRP1 have escaped detection by conventional methods
up to now However, this study shows that protein–
protein interactions occur with human IRP1 and they
should be considered to elucidate the molecular details
of the ‘iron–sulfur switch’ regulating this protein in
metazoan cells
Experimental procedures
Strains and media
Strains W303-1B (mat a, ade2–1, ura3–1, his3–11, trp1–1,
leu2–3,112, can1–100), FYF4-A1 aco1 (mat a, ura3–52,
trp1D63, leu2D1, his3D200, aco1::URA3), and BY4741
(mat a, his3, leu2, met15, ura3) were used in the reported
experiments Synthetic media lacking leucine or uracil and
containing either 2% glucose or 2% raffinose were used to
grow cells Expression plasmids YEpLIRP1, YEpUIRP1,
YEpLmitIRP1 and YEpUmitIRP1 were designed to
produce human cytosolic aconitase in the yeast cytosol
(c-hIRP1) or mitochondria (m-hIRP1) from the yeast
mito-chondrial aconitase presequence Similarly, YEpLaco2C
and YEpLaco2P target human mitochondrial aconitase in
the yeast cytosol (c-hAco2) and mitochondria from its own
presequence (m-hAco2), respectively YEpLacoPS– and
YEpLScmaco do so for yeast mitochondrial aconitase,
pro-ducing c-yAco1 and m-yAco1, respectively U and L refer
to uracil and leucine selection markers
For assays evaluating complementation of glutamate
auxotrophy, FYF4-A1 aco1::ura3 cells containing the
plas-mid of interest were grown in synthetic minimal medium
devoid of selected amino acids at 30C Cell concentrations
were determined with a Bright-Line hematocytometer
(Hausser Scientific, Horsham, PA) and the indicated
num-bers of cells were spotted on selective agar plates supple-mented with glutamate or not Assays were carried out at least three times with independent transformants and repre-sentative results are shown
Freezing and freeze substitution for ultrastructural studies
Yeast pellets were placed on the surface of a copper electron microscopy grid (400 mesh) that had been coated with form-var Each loop was very quickly submersed in liquid pro-pane, precooled and held at )180 C using liquid nitrogen cooling Loops were then transferred in a precooled solution
of 4% osmium tetraoxide in dry acetone in a 1.8 mL poly-propylene vial at )82 C for 48 h (substitution fixation), warmed gradually to room temperature, followed by three washes in dry acetone Specimens were stained for 1 h in 1% uranyl acetate in acetone at 4C, in the dark After another rinse in dry acetone, samples were embedded pro-gressively with araldite (epoxy resin Fluka, Buchs, Switzer-land) Ultrathin sections were contrasted with lead citrate
Immunogold electron microscopy Yeast cells were cryofixed as described for ultrastructural studies and freeze-substituted with acetone plus 0.2% glu-taraldehyde for 3 days at )82 C Samples were rinsed with acetone at )20 C, embedded progressively at )20 C in
LR Gold resin (EMS, Fort Washington, PA) Resin poly-merization was carried out at)20 C for 7 days under UV illumination Ultrathin LR Gold sections were collected on nickel grids coated with formvar Sections were first incuba-ted for 5 min with 1 mgÆmL)1glycine and 5 min with fetal bovine serum (1 : 20) The grids were incubated 45 min at room temperature with anti-(human IRP1) or anti-(yeast a-conitase) serum diluted 1 : 500 or 1 : 250 v⁄ v, rinsed with NaCl⁄ Tris: 0.1% BSA and then incubated for 45 min at room temperature with anti-rabbit IgG conjugated to
10 nm gold particles (BioCell International, Cardiff, UK) The sections were rinsed with distilled water, contrasted
5 min with 2% uranyl acetate in water, followed by 1% lead citrate for 1 min Specimens were observed with a Phi-lips Tecnai 12 Biotwin (120 kV) electron microscope (SER-COMI, Universite´ Victor Segalen Bordeaux 2, France)
Purification of mitochondrial inclusion bodies on sucrose gradient
Mitochondria were purified from 1 L of a W303-1B raffi-nose culture producing m-hIRP1 Fifty microlitres of the resulting mitochondria were resuspended in 250 lL of
Tris-Cl 10 mm, pH 7.4 containing 10 lL of a protease inhibitor cocktail (Sigma, St Louis, MO) Ultrasonic irradiation of the suspension was carried out at 60 W, 4C during 10 s
Trang 9using a SONICS Vibra cell sonicator (Fisher Bioblock
Sci-entific, Illkirch, France) with a 3-mm tip One hundred and
twenty microlitres of the lysate were applied to a 5 mL
dis-continuous sucrose gradient (20, 30, 50, 60, 80%) prepared
in Tris⁄ Cl 10 mm, pH 7.4 Sedimentation was carried out
with a SW65 Beckman rotor, at 4C and 50 000 r.p.m for
3 h Five hundred-microlitre fractions were collected from
the top of the gradient and 20 lL of each fraction were
analysed on SDS⁄ PAGE with silver staining [25] or
Coo-massie Brilliant Blue staining for MS analysis
Immunoblotting and protein transfer for amino
acid sequencing
Proteins were transferred on nitrocellulose membrane
(Bio-Rad, Hercules, CA) for western blot analysis Polyclonal
antibodies were raised against human recombinant IRP1
[13] Polyclonal antibodies against yAco1 were a kind gift
of G Lauquin (IBGC-Bordeaux, France) Bands were
visu-alized using Alexa Fluor 488 secondary antibodies
(Invitro-gen, Carlsbad, CA) and a FluoroImager (Molecular
Dynamics, GE Healthcare Life Sciences, Piscataway, NJ)
with the 530 ± 30 nm filter For amino acid sequencing,
proteins were transferred to Immobilon-P membranes
(Mil-lipore, Billerica, MA) Protein bands were visualized by
Coomassie Brilliant Blue staining, cut and stored at)20 C
until used
N-Terminal amino acid sequencing
Amino acid sequence determination based on Edman
degradation was performed using an Applied Biosystems
(Foster City, CA, USA) gas-phase sequencer model 492
Phenylthiohydantoin amino acid derivatives generated at
each sequencing cycle were identified and quantified
on-line with an Applied Biosystems Model 140C HPLC
sys-tem using the data analysis syssys-tem protein sequencing
from Applied Biosystems Model 610A (software version
2.1) The procedure and reagents used were as
recommen-ded by the manufacturer Chromatography was used to
identify and quantify the derivatized amino acid removed
at each sequencing cycle Retention times and integration
values of peaks were compared to the chromatographic
profile obtained for a standard mixture of derivatized
amino acids (Perkin–Elmer standard kit; Wellesley, MA,
USA)
Measurements of metal concentrations
Ten microlitres of purified mitochondria (10 mgÆmL)1) were
dried 48 h at room temperature and mineralized with nitric
acid Metal content was determined using inductively
cou-pled plasma-atomic emission spectrometry (Vista MPX,
Varian SA, Les Ulis, France)
In-gel digestion of proteins, peptide extraction and MS analysis
Protein bands were excised from the Coomassie Brilliant Blue-stained gel and subjected to tryptic digestion as des-cribed previously [26] The dried gel-extracted tryptic pep-tides were solubilized in 95% water (v⁄ v) containing 2.5% acetonitrile and 2.5% trifluoroacetic acid for
nanoLC-MS⁄ MS analysis (CapLC and Q-TOF Ultima, Waters, Mil-ford, MA) The method consisted in a 50 min run at a flow rate of 200 nLÆmin)1 using a two-solvent gradient: sol-vent A (acetonitrile⁄ water ⁄ formic acid 2 : 97.9 : 0.1 v ⁄ v ⁄ v) and solvent B (acetonitrile⁄ water ⁄ formic acid 80 : 19.9 : 0.1
v⁄ v ⁄ v) The system includes a 300 lm · 5 mm PepMap C18
precolumn (LC-Packings, Dionex, Sunnivale) to concen-trate peptides before injection onto a 75 lm· 150 mm C18
column (LC-Packings) directly coupled to the mass spectro-meter Proteins were identified from MS⁄ MS data with mascot2.0 software (Matrix Science, http://www.matrix-science.com) Database searches were performed on the SwissProt_Trembl databank
Acknowledgements
We thank Guy Lauquin (University of Bordeaux), Pierre-Louis Blaiseau (Institut Monod, Paris), and Ge´rard Brandolin (CNRS-CEA, Grenoble) for materi-als used in this study This study was partly supported
by a grant of the Programme de Toxicologie Nucle´aire from CEA
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