membrane protein protocols - barry s selinsky

... Cells overexpressing this protein are broken by vigorous shaking with glass beads and a total membrane fraction is prepared from these cells for the characteriza- tion of full-length SR-BI by SDS-PAGE ... expressed in tissues other than brown adipose tissue and provide potential therapeutic targets for the treatment of metabolic diseases such as dia- betes and obesity and of inflammatory d...

Ngày tải lên: 08/04/2014, 12:51

... is a proline- and glycine-rich transmembrane protein, and may thus be classified as a simple sequence protein (SSP). This type of sequence is fre- quent in organisms having G + C-rich genomes, ... membrane proteins present on the bacillus and its host [8]. The M. tuberculosis cell membrane, and that of other prokaryotes, contains proteins func- tioning as enzymes, ligands, signal transd...

Ngày tải lên: 18/02/2014, 16:20

... RL-pS-PPSSSAASS-pY-SFSDLNSTR (aa res- idues 47–68), which is phosphorylated at S- 49 and Y-59. The serine phosphorylation site, unique for human emerin, reflects probably a basal phosphoryla- tion ... RL-pS-PPSSSAASS-pY-SFSDLNSTR (aa residues 47–68), which is phosphorylat- ed at S- 49 and Y-59, obtained by using the SILAC approach. In both figure parts, the phosphotyrosine-specific mass differ...

Ngày tải lên: 07/03/2014, 12:20

... proteins have suggested the pos- sibility of nonsequential insertion mechanisms, where interactions between neighboring transmembrane seg- ments or re-orientation of transmembrane segments during ... be that N-tails are so rapidly trans- located across the membrane that some chains pass OST too fast to be glycosylated and only become modified in a slower, post-translational process. The final...

Ngày tải lên: 07/03/2014, 16:20

... O, Stern DM, Hartmann E, Ogawa S & Tohyama M (1999) Stress-associated endoplasmic reticulum protein 1 (Serp1) ⁄ ribosome- associated membrane protein 4 (Ramp4) stabilizes membrane proteins ... the details of the mechanism remain unknown. PAT-10 was discovered as a translocon-associated protein dur- ing a search for Sec61 partners during opsin nascent chain insertion [50]. It i...

Ngày tải lên: 22/03/2014, 15:21

... cells (Fig. 8). Therefore, it was suggested that COS-7 cells secreted exosome-like membrane vesicles and that MFG-E8 was secreted as a complex with the exosome-like membrane vesicles. It was also ... that COMMA-1D cells endogenously expressed MFG-E8 -S and a small amount of MFG-E8-L mRNAs [9]. To clarify whether MFG-E8 expressed in COMMA-1D cells was secreted with membrane vesicles, th...

Ngày tải lên: 24/03/2014, 03:21

... analysis has been particularly useful for the study of genes that would be lethal in a homo- zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens ... Pax-1 in this fused somite is less than in the normal contra-lateral somites. (C) A hematoxylin-eosin stained horizontal section of a chick embryo displaying a Pax-1 antisense ODN-induced somite...

Ngày tải lên: 08/04/2014, 12:56

... differentiated tumors (17). 4. Anti-CD31 is commonly used for visualizing vascularization in tissue sec- tions (18,19). 5. In some tissues, expression of a determined adhesion molecule is associated with malignant ... alcohol; six-well culture plates; 11 × 22 mm glass coverslips (Thomas Scientific, Swedesboro NJ). 3. Tweezers for handling the coverslips. 2.2. Transfection of COS Cells on Cove...

Ngày tải lên: 10/04/2014, 22:13

... facilities necessary for handling of radioisotopes. 3.1. Assembly of the Flow-Dialysis Cell When everything is ready to begin, the flow-dialysis cell, which has been stored as disassembled parts, is ... cellulose dialysis tubing is prepared for use as a dialy- sis membrane. Select the appropriate membrane considering the rate of dialysis, as well as the pore size to keep proteins and effec...

Ngày tải lên: 11/04/2014, 00:23