Statistical Analysis of fold changes in lung homogenate cytokine concentrations measured by multiplex cytokine assay

Một phần của tài liệu MURINE MODELS TO STUDY IMMUNITY AND IMMUNISATION AGAINST RESPIRATORY VIRAL PATHOGENS (Trang 318 - 339)

III. ABSTRACTS AND POSTERS / ORAL PRESENTATIONS

6. Mice deficient in interferon regulatory factor 4 (IRF4) are more susceptible to infection with mouse-adapted influenza A/Aichi/2/68

8.8 Statistical Analysis of fold changes in lung homogenate cytokine concentrations measured by multiplex cytokine assay

G-CSF

Descriptivesa Fold Change

13 113.51701 211.621876 58.693348 -14.36481 241.39883 17.161 809.238 13 81.73654 68.809139 19.084222 40.15559 123.31749 25.857 290.382 13 101.19786 69.655883 19.319066 59.10524 143.29049 26.193 198.324 39 98.81714 131.701518 21.089121 56.12445 141.50983 17.161 809.238 -/-

+/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = G-CSF a.

ANOVAa Fold Change

6675.513 2 3337.757 .184 .833

652445.5 36 18123.486

659121.0 38

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = G-CSF a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

31.780474 52.803680 1.000 -100.81170 164.37265 12.319150 52.803680 1.000 -120.27302 144.91132 -31.780474 52.803680 1.000 -164.37265 100.81170 -19.461324 52.803680 1.000 -152.05350 113.13085 -12.319150 52.803680 1.000 -144.91132 120.27302 19.461324 52.803680 1.000 -113.13085 152.05350 (J) genoty pe

+/- +/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = G-CSF a.

299 GM-CSF

Descriptivesa Fold Change

14 7.17439 2.858574 .763986 5.52390 8.82488 2.775 12.553

13 2.20824 .863141 .239392 1.68665 2.72983 1.242 4.343

14 2.75709 1.507686 .402946 1.88657 3.62760 .842 6.189

41 4.09141 2.953027 .461185 3.15932 5.02350 .842 12.553

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = GM-CSF a.

ANOVAa Fold Change

204.095 2 102.048 26.795 .000

144.720 38 3.808

348.815 40

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = GM-CSF a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

4.966154* .751654 .000 3.08355 6.84876

4.417305* .737603 .000 2.56989 6.26472

-4.966154* .751654 .000 -6.84876 -3.08355

-.548849 .751654 1.000 -2.43145 1.33375

-4.417305* .737603 .000 -6.26472 -2.56989

.548849 .751654 1.000 -1.33375 2.43145

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = GM-CSF a.

300 IFN-gamma

Descriptivesa Fold Change

13 7.71162 8.108790 2.248974 2.81153 12.61171 2.126 32.475

12 19.58692 21.959014 6.339021 5.63483 33.53902 5.040 85.125

12 45.08713 35.704951 10.307132 22.40129 67.77298 15.071 134.594 37 23.68486 28.446623 4.676596 14.20028 33.16943 2.126 134.594 -/-

+/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IFN-gamma a.

ANOVAa Fold Change

9015.082 2 4507.541 7.618 .002

20116.490 34 591.661

29131.572 36

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IFN-gamma a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-11.875307 9.737430 .693 -36.39668 12.64607

-37.375515* 9.737430 .002 -61.89689 -12.85414

11.875307 9.737430 .693 -12.64607 36.39668

-25.500208* 9.930269 .044 -50.50720 -.49322

37.375515* 9.737430 .002 12.85414 61.89689

25.500208* 9.930269 .044 .49322 50.50720

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IFN-gamma a.

301 IL-10

Descriptivesa Fold Change

14 14.38794 12.356027 3.302287 7.25378 21.52210 1.386 46.980

14 6.67941 2.496145 .667123 5.23818 8.12064 1.246 11.702

8 5.07771 1.528971 .540573 3.79946 6.35596 2.827 6.976

36 9.32124 8.755835 1.459306 6.35869 12.28379 1.246 46.980

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-10 a.

ANOVAa Fold Change

601.170 2 300.585 4.764 .015

2082.092 33 63.094

2683.263 35

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-10 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

7.708530* 3.002230 .045 .13627 15.28079

9.310232* 3.520427 .037 .43097 18.18950

-7.708530* 3.002230 .045 -15.28079 -.13627

1.601702 3.520427 1.000 -7.27756 10.48097

-9.310232* 3.520427 .037 -18.18950 -.43097

-1.601702 3.520427 1.000 -10.48097 7.27756

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-10 a.

302 IL-12 p40

Descriptivesa Fold Change

1 .11241 . . . . .112 .112

3 1.23077 .477843 .275883 .04374 2.41780 .690 1.597

2 1.29589 .046330 .032761 .87963 1.71215 1.263 1.329

6 1.06608 .557726 .227691 .48079 1.65138 .112 1.597

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-12 p40 a.

ANOVAa Fold Change

1.096 2 .548 3.585 .160

.459 3 .153

1.555 5

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-12 p40 a.

303 IL-12 p70

Descriptivesa Fold Change

10 2.38134 2.066481 .653479 .90307 3.85962 .061 5.653

7 .95809 .174903 .066107 .79633 1.11985 .604 1.105

11 1.09752 .078951 .023805 1.04449 1.15056 .941 1.190

28 1.52117 1.364519 .257870 .99207 2.05028 .061 5.653

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-12 p70 a.

ANOVAa Fold Change

11.593 2 5.796 3.746 .038

38.679 25 1.547

50.272 27

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-12 p70 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

1.423255 .612975 .086 -.14963 2.99614

1.283819 .543477 .079 -.11073 2.67837

-1.423255 .612975 .086 -2.99614 .14963

-.139436 .601393 1.000 -1.68260 1.40373

-1.283819 .543477 .079 -2.67837 .11073

.139436 .601393 1.000 -1.40373 1.68260

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = IL-12 p70 a.

304 IL-13

Descriptivesa Fold Change

12 1.51823 .553184 .159690 1.16676 1.86971 .888 2.665

12 .74440 1.310256 .378238 -.08810 1.57689 .011 3.654

12 1.03639 1.237607 .357266 .25005 1.82272 .005 3.807

36 1.09967 1.105364 .184227 .72567 1.47367 .005 3.807

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-13 a.

ANOVAa Fold Change

3.665 2 1.833 1.547 .228

39.099 33 1.185

42.764 35

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-13 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

.773835 .444376 .273 -.34697 1.89465

.481847 .444376 .858 -.63896 1.60266

-.773835 .444376 .273 -1.89465 .34697

-.291989 .444376 1.000 -1.41280 .82882

-.481847 .444376 .858 -1.60266 .63896

.291989 .444376 1.000 -.82882 1.41280

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = IL-13 a.

305 IL-17

Descriptivesa Fold Change

14 1.54399 .609871 .162995 1.19186 1.89612 .734 2.863

12 .70847 .254971 .073604 .54647 .87047 .252 1.118

14 .77036 .397830 .106325 .54066 1.00006 .087 1.334

40 1.02257 .588100 .092987 .83448 1.21065 .087 2.863

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-17 a.

ANOVAa Fold Change

5.881 2 2.940 14.300 .000

7.608 37 .206

13.489 39

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-17 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

.835521* .178387 .000 .38817 1.28287

.773629* .171388 .000 .34383 1.20343

-.835521* .178387 .000 -1.28287 -.38817

-.061893 .178387 1.000 -.50924 .38545

-.773629* .171388 .000 -1.20343 -.34383

.061893 .178387 1.000 -.38545 .50924

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-17 a.

306 IL-1a

Descriptivesa Fold Change

10 1.52710 1.014902 .320940 .80109 2.25312 .591 3.379

10 .52118 .604917 .191292 .08844 .95391 .006 1.858

12 1.40974 .904029 .260971 .83535 1.98413 .269 2.832

32 1.16874 .945757 .167188 .82776 1.50972 .006 3.379

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-1a a.

ANOVAa Fold Change

6.175 2 3.087 4.154 .026

21.554 29 .743

27.728 31

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-1a a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

1.005928* .385545 .043 .02629 1.98556

.117364 .369131 1.000 -.82056 1.05529

-1.005928* .385545 .043 -1.98556 -.02629

-.888563 .369131 .068 -1.82649 .04937

-.117364 .369131 1.000 -1.05529 .82056

.888563 .369131 .068 -.04937 1.82649

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/- +/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-1a a.

307 IL-1b

Descriptivesa Fold Change

13 6.35674 2.738818 .759611 4.70169 8.01179 3.553 13.618

11 7.81745 9.538129 2.875854 1.40965 14.22525 1.076 24.596

13 22.85307 22.311067 6.187977 9.37063 36.33551 1.514 69.067

37 12.58701 15.897344 2.613507 7.28657 17.88745 1.076 69.067

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-1b a.

ANOVAa Fold Change

2124.943 2 1062.471 5.180 .011

6973.177 34 205.093

9098.120 36

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-1b a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-1.460713 5.866965 1.000 -16.23525 13.31383

-16.496331* 5.617191 .018 -30.64187 -2.35079

1.460713 5.866965 1.000 -13.31383 16.23525

-15.035618* 5.866965 .045 -29.81016 -.26108

16.496331* 5.617191 .018 2.35079 30.64187

15.035618* 5.866965 .045 .26108 29.81016

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-1b a.

308 IL-2

Descriptivesa Fold Change

7 .75092 .508825 .192318 .28033 1.22150 .037 1.688

14 2.22455 1.769980 .473047 1.20260 3.24651 .342 5.943

21 1.73334 1.618864 .353265 .99644 2.47024 .037 5.943

+/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-2 a.

ANOVAa Fold Change

10.134 1 10.134 4.554 .046

42.280 19 2.225

52.414 20

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-2 a.

309 IL-3

Descriptivesa Fold Change

14 10.64912 5.131322 1.371403 7.68638 13.61186 3.285 19.499

13 5.44930 3.019112 .837351 3.62487 7.27373 .454 12.729

14 4.36573 1.789872 .478363 3.33229 5.39917 1.749 7.995

41 6.85485 4.492486 .701608 5.43684 8.27285 .454 19.499

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-3 a.

ANOVAa Fold Change

313.973 2 156.987 12.092 .000

493.324 38 12.982

807.297 40

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-3 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

5.199822* 1.387779 .002 1.72397 8.67567

6.283390* 1.361837 .000 2.87251 9.69427

-5.199822* 1.387779 .002 -8.67567 -1.72397

1.083568 1.387779 1.000 -2.39228 4.55942

-6.283390* 1.361837 .000 -9.69427 -2.87251

-1.083568 1.387779 1.000 -4.55942 2.39228

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-3 a.

310 IL-4

Descriptivesa Fold Change

14 9.44814 3.612702 .965535 7.36223 11.53405 2.918 16.260

14 1.69335 1.009233 .269729 1.11064 2.27606 .176 3.506

14 1.92515 .922024 .246421 1.39279 2.45751 .302 3.338

42 4.35555 4.245412 .655081 3.03258 5.67851 .176 16.260

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-4 a.

ANOVAa Fold Change

545.001 2 272.500 54.791 .000

193.964 39 4.973

738.965 41

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-4 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

7.754791* .842906 .000 5.64613 9.86345

7.522988* .842906 .000 5.41433 9.63165

-7.754791* .842906 .000 -9.86345 -5.64613

-.231803 .842906 1.000 -2.34046 1.87686

-7.522988* .842906 .000 -9.63165 -5.41433

.231803 .842906 1.000 -1.87686 2.34046

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-4 a.

311 IL-5

Descriptivesa Fold Change

13 21.01575 25.340013 7.028055 5.70294 36.32857 2.876 77.769

14 3.30235 3.295517 .880764 1.39958 5.20513 .029 12.297

14 15.09350 23.718675 6.339083 1.39875 28.78826 .983 89.508

41 12.94504 20.840585 3.254753 6.36694 19.52314 .029 89.508

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-5 a.

ANOVAa Fold Change

2213.136 2 1106.568 2.774 .075

15160.063 38 398.949

17373.199 40

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-5 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

17.713400 7.693162 .081 -1.55500 36.98180

5.922250 7.693162 1.000 -13.34615 25.19065

-17.713400 7.693162 .081 -36.98180 1.55500

-11.791150 7.549352 .380 -30.69936 7.11706

-5.922250 7.693162 1.000 -25.19065 13.34615

11.791150 7.549352 .380 -7.11706 30.69936

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = IL-5 a.

312 IL-6

Descriptivesa Fold Change

14 57.21429 69.640141 18.612111 17.00527 97.42331 1.335 274.873

13 76.65380 72.963893 20.236543 32.56217 120.74544 7.576 228.752 14 286.29464 280.120515 74.865357 124.55787 448.03141 33.792 1020.611 41 141.60060 199.665219 31.182468 78.57848 204.62272 1.335 1020.611 -/-

+/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-6 a.

ANOVAa Fold Change

447638.9 2 223819.473 7.415 .002

1147009 38 30184.448

1594648 40

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-6 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-19.439519 66.917207 1.000 -187.04128 148.16224 -229.08036* 65.666308 .004 -393.54910 -64.61162 19.439519 66.917207 1.000 -148.16224 187.04128 -209.64084* 66.917207 .010 -377.24260 -42.03908 229.080357* 65.666308 .004 64.61162 393.54910 209.640838* 66.917207 .010 42.03908 377.24260 (J) genoty pe

+/- +/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = IL-6 a.

313 IL-9

Descriptivesa Fold Change

14 .89428 .251000 .067083 .74935 1.03920 .498 1.422

10 .65733 1.337433 .422933 -.29942 1.61407 .021 3.477

9 1.52002 2.162622 .720874 -.14232 3.18235 .006 5.716

33 .99313 1.347404 .234553 .51536 1.47090 .006 5.716

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = IL-9 a.

ANOVAa Fold Change

3.763 2 1.881 1.039 .366

54.333 30 1.811

58.096 32

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = IL-9 a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

.236950 .557202 1.000 -1.17597 1.64987

-.625740 .574976 .855 -2.08373 .83225

-.236950 .557202 1.000 -1.64987 1.17597

-.862690 .618339 .520 -2.43064 .70526

.625740 .574976 .855 -.83225 2.08373

.862690 .618339 .520 -.70526 2.43064

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = IL-9 a.

314 KC

Descriptivesa Fold Change

13 3.27493 1.754237 .486538 2.21485 4.33500 .404 6.500

13 8.60980 7.557836 2.096167 4.04264 13.17695 1.750 23.431

14 13.67384 10.474268 2.799366 7.62618 19.72151 2.075 33.904

40 8.64838 8.589750 1.358159 5.90125 11.39552 .404 33.904

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = KC a.

ANOVAa Fold Change

728.956 2 364.478 6.276 .004

2148.613 37 58.071

2877.568 39

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = KC a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-5.334874 2.988970 .247 -12.83042 2.16067

-10.398919* 2.935110 .003 -17.75940 -3.03844

5.334874 2.988970 .247 -2.16067 12.83042

-5.064045 2.935110 .278 -12.42453 2.29644

10.398919* 2.935110 .003 3.03844 17.75940

5.064045 2.935110 .278 -2.29644 12.42453

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = KC a.

315 MIP-1a

Descriptivesa Fold Change

13 9.55345 7.945246 2.203615 4.75218 14.35471 2.920 33.690

7 10.39606 2.060396 .778756 8.49051 12.30161 7.434 12.847

2 2.00744 .754429 .533462 -4.77084 8.78572 1.474 2.541

22 9.13555 6.541356 1.394622 6.23528 12.03582 1.474 33.690

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = MIP-1a a.

ANOVAa Fold Change

115.012 2 57.506 1.394 .272

783.564 19 41.240

898.576 21

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = MIP-1a a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-.842611 3.010611 1.000 -8.74578 7.06056

7.546006 4.877748 .415 -5.25860 20.35061

.842611 3.010611 1.000 -7.06056 8.74578

8.388617 5.148938 .359 -5.12789 21.90512

-7.546006 4.877748 .415 -20.35061 5.25860

-8.388617 5.148938 .359 -21.90512 5.12789

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = MIP-1a a.

316 MIP-1b

Descriptivesa Fold Change

13 5.06358 1.008175 .279617 4.45434 5.67281 3.090 6.971

8 8.11074 3.004582 1.062280 5.59885 10.62264 2.295 12.221

2 4.13192 .945869 .668830 -4.36638 12.63021 3.463 4.801

23 6.04245 2.433326 .507384 4.99020 7.09470 2.295 12.221

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = MIP-1b a.

ANOVAa Fold Change

53.979 2 26.990 7.076 .005

76.284 20 3.814

130.264 22

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = MIP-1b a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

-3.047164* .877598 .007 -5.33996 -.75436

.931662 1.483411 1.000 -2.94388 4.80720

3.047164* .877598 .007 .75436 5.33996

3.978826 1.543983 .054 -.05496 8.01261

-.931662 1.483411 1.000 -4.80720 2.94388

-3.978826 1.543983 .054 -8.01261 .05496

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = MIP-1b a.

317 RANTES

Descriptivesa Fold Change

10 5.62859 4.129808 1.305960 2.67430 8.58287 1.157 12.083

13 4.87179 5.206986 1.444158 1.72524 8.01834 .161 18.690

13 2.81443 1.440841 .399617 1.94374 3.68512 .959 5.408

36 4.33908 3.979588 .663265 2.99258 5.68558 .161 18.690

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = RANTES a.

ANOVAa Fold Change

50.537 2 25.268 1.655 .207

503.762 33 15.266

554.299 35

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = RANTES a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

.756797 1.643419 1.000 -3.38825 4.90185

2.814158 1.643419 .289 -1.33089 6.95921

-.756797 1.643419 1.000 -4.90185 3.38825

2.057361 1.532496 .566 -1.80792 5.92264

-2.814158 1.643419 .289 -6.95921 1.33089

-2.057361 1.532496 .566 -5.92264 1.80792

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

group = RANTES a.

318 TNF-A

Descriptivesa Fold Change

13 7.70673 8.939466 2.479362 2.30467 13.10880 1.830 34.314

13 2.55600 2.233045 .619335 1.20658 3.90541 .517 8.590

13 2.39292 1.435040 .398008 1.52573 3.26010 .540 4.975

39 4.21855 5.805977 .929700 2.33647 6.10063 .517 34.314

-/- +/- +/+

Total

N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or

Mean

Minimum Maximum

group = TNF-A a.

ANOVAa Fold Change

237.438 2 118.719 4.096 .025

1043.519 36 28.987

1280.956 38

Between Groups Within Groups Total

Sum of

Squares df Mean Square F Sig.

group = TNF-A a.

Post Hoc Tests

Multi ple Comparisonsa Dependent Variable: Fold Change

Bonf erroni

5.150736 2.111748 .059 -.15195 10.45342

5.313815* 2.111748 .049 .01113 10.61650

-5.150736 2.111748 .059 -10.45342 .15195

.163079 2.111748 1.000 -5.13961 5.46576

-5.313815* 2.111748 .049 -10.61650 -.01113

-.163079 2.111748 1.000 -5.46576 5.13961

(J) genoty pe +/-

+/+

-/- +/+

-/- +/- (I) genot y pe -/-

+/-

+/+

Mean Dif f erence

(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al

The mean dif f erence is signif icant at the . 05 lev el.

*.

group = TNF-A a.

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