III. ABSTRACTS AND POSTERS / ORAL PRESENTATIONS
6. Mice deficient in interferon regulatory factor 4 (IRF4) are more susceptible to infection with mouse-adapted influenza A/Aichi/2/68
8.11 Statistical analysis of gene expression in lung homogenate measured by Quantitative Real-Time PCR
IFN Alpha
Descriptivesa ACTB
28 .164 .4336 .0819 -.004 .332 .0 2.3
28 .652 1.5312 .2894 .058 1.245 .0 6.9
32 .277 .8184 .1447 -.018 .572 .0 4.4
88 .360 1.0329 .1101 .141 .579 .0 6.9
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IFN Alpha a.
ANOVAa ACTB
3.677 2 1.838 1.753 .179
89.148 85 1.049
92.824 87
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IFN Alpha a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.4877 .2737 .235 -1.156 .181
-.1135 .2650 1.000 -.761 .534
.4877 .2737 .235 -.181 1.156
.3742 .2650 .485 -.273 1.021
.1135 .2650 1.000 -.534 .761
-.3742 .2650 .485 -1.021 .273
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
gene = IFN Alpha a.
327 IFN Beta
Descriptivesa ACTB
35 .031 .1803 .0305 -.031 .093 .0 1.1
30 .119 .1969 .0359 .045 .192 .0 .9
33 .067 .1112 .0194 .028 .107 .0 .4
98 .070 .1684 .0170 .036 .104 .0 1.1
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IFN Beta a.
ANOVAa ACTB
.126 2 .063 2.275 .108
2.625 95 .028
2.751 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IFN Beta a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.0881 .0414 .107 -.189 .013
-.0366 .0403 1.000 -.135 .062
.0881 .0414 .107 -.013 .189
.0515 .0419 .668 -.051 .154
.0366 .0403 1.000 -.062 .135
-.0515 .0419 .668 -.154 .051
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
gene = IFN Beta a.
328 IFN Gamma
Descriptivesa ACTB
36 2.428 2.6749 .4458 1.523 3.333 .0 9.7
29 4.302 3.7426 .6950 2.878 5.725 .8 20.4
31 89.850 44.0350 7.9089 73.698 106.002 32.2 192.8
96 31.224 47.7090 4.8693 21.557 40.891 .0 192.8
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IFN Gamma a.
ANOVAa ACTB
157418.9 2 78709.426 124.458 .000
58814.984 93 632.419
216233.8 95
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IFN Gamma a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-1.8739 6.2749 1.000 -17.173 13.425
-87.4222* 6.1618 .000 -102.445 -72.399
1.8739 6.2749 1.000 -13.425 17.173
-85.5483* 6.4968 .000 -101.388 -69.709
87.4222* 6.1618 .000 72.399 102.445
85.5483* 6.4968 .000 69.709 101.388
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IFN Gamma a.
329 IRF-1
Descriptivesa ACTB
36 .709 .9479 .1580 .388 1.030 .0 4.2
30 2.003 2.0195 .3687 1.249 2.757 .1 9.1
33 4.087 3.9681 .6908 2.680 5.494 .4 17.7
99 2.227 2.9488 .2964 1.639 2.815 .0 17.7
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF1 a.
ANOVAa ACTB
198.591 2 99.295 14.585 .000
653.582 96 6.808
852.173 98
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF1 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-1.2936 .6450 .143 -2.865 .278
-3.3777* .6288 .000 -4.910 -1.845
1.2936 .6450 .143 -.278 2.865
-2.0841* .6582 .006 -3.688 -.480
3.3777* .6288 .000 1.845 4.910
2.0841* .6582 .006 .480 3.688
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF1 a.
330 IRF-3
Descriptivesa ACTB
36 .132 .1162 .0194 .092 .171 .0 .6
30 .549 .4264 .0778 .390 .708 .1 1.6
33 .518 .4392 .0764 .363 .674 .1 2.3
99 .387 .3992 .0401 .307 .467 .0 2.3
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF3 a.
ANOVAa ACTB
3.703 2 1.851 14.917 .000
11.916 96 .124
15.619 98
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF3 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.4173* .0871 .000 -.630 -.205
-.3866* .0849 .000 -.594 -.180
.4173* .0871 .000 .205 .630
.0307 .0889 1.000 -.186 .247
.3866* .0849 .000 .180 .594
-.0307 .0889 1.000 -.247 .186
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF3 a.
331 IRF-4
Descriptivesa ACTB
27 .228 .8153 .1569 -.095 .551 .0 4.1
30 .515 .8988 .1641 .179 .850 .0 4.8
33 1.085 1.2656 .2203 .636 1.534 .2 6.7
90 .638 1.0787 .1137 .412 .864 .0 6.7
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF4 a.
ANOVAa ACTB
11.582 2 5.791 5.478 .006
91.971 87 1.057
103.553 89
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF4 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.2868 .2727 .888 -.953 .379
-.8568* .2668 .006 -1.508 -.205
.2868 .2727 .888 -.379 .953
-.5700 .2594 .092 -1.203 .063
.8568* .2668 .006 .205 1.508
.5700 .2594 .092 -.063 1.203
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF4 a.
332 IRF-5
Descriptivesa ACTB
36 .402 .4024 .0671 .265 .538 .0 2.0
27 2.129 1.3021 .2506 1.614 2.644 .4 6.2
32 3.023 1.2832 .2268 2.561 3.486 1.6 6.3
95 1.776 1.5365 .1576 1.463 2.089 .0 6.3
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF5 a.
ANOVAa ACTB
121.135 2 60.568 55.283 .000
100.794 92 1.096
221.929 94
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF5 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-1.7271* .2665 .000 -2.377 -1.077
-2.6216* .2543 .000 -3.242 -2.001
1.7271* .2665 .000 1.077 2.377
-.8945* .2735 .005 -1.562 -.228
2.6216* .2543 .000 2.001 3.242
.8945* .2735 .005 .228 1.562
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF5 a.
333 IRF-7
Descriptivesa ACTB
36 1.718 2.1121 .3520 1.004 2.433 .0 10.2
30 8.563 7.4828 1.3662 5.768 11.357 .8 24.2
33 18.420 14.6363 2.5478 13.230 23.610 2.1 59.4
99 9.359 11.7216 1.1781 7.022 11.697 .0 59.4
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF7 a.
ANOVAa ACTB
4829.874 2 2414.937 26.848 .000
8634.965 96 89.948
13464.839 98
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF7 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-6.8442* 2.3445 .013 -12.557 -1.131
-16.7014* 2.2857 .000 -22.271 -11.132
6.8442* 2.3445 .013 1.131 12.557
-9.8571* 2.3925 .000 -15.687 -4.027
16.7014* 2.2857 .000 11.132 22.271
9.8571* 2.3925 .000 4.027 15.687
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF7 a.
334 IRF-8
Descriptivesa ACTB
36 .321 .2287 .0381 .244 .399 .0 1.0
29 1.311 .9142 .1698 .963 1.658 .4 3.6
33 2.020 1.2841 .2235 1.565 2.476 .6 4.6
98 1.186 1.1502 .1162 .956 1.417 .0 4.6
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = IRF8 a.
ANOVAa ACTB
50.339 2 25.169 30.656 .000
77.998 95 .821
128.337 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = IRF8 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.9892* .2261 .000 -1.540 -.438
-1.6990* .2184 .000 -2.231 -1.167
.9892* .2261 .000 .438 1.540
-.7098* .2306 .008 -1.272 -.148
1.6990* .2184 .000 1.167 2.231
.7098* .2306 .008 .148 1.272
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = IRF8 a.
335 MyD88
Descriptivesa ACTB
36 .344 .3173 .0529 .237 .452 .1 1.7
29 1.619 1.3673 .2539 1.099 2.139 .3 5.8
33 2.348 1.7750 .3090 1.719 2.978 .5 8.4
98 1.396 1.5327 .1548 1.089 1.704 .1 8.4
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = My D88 a.
ANOVAa ACTB
71.194 2 35.597 21.582 .000
156.688 95 1.649
227.882 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = My D88 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-1.2744* .3205 .000 -2.055 -.493
-2.0042* .3095 .000 -2.758 -1.250
1.2744* .3205 .000 .493 2.055
-.7298 .3269 .084 -1.526 .067
2.0042* .3095 .000 1.250 2.758
.7298 .3269 .084 -.067 1.526
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = My D88 a.
336 NFKB
Descriptivesa ACTB
36 .262 .2973 .0495 .162 .363 .0 1.6
29 .763 .4849 .0900 .578 .947 .1 2.0
33 1.410 1.5461 .2691 .862 1.958 .0 8.1
98 .797 1.0596 .1070 .584 1.009 .0 8.1
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = NFKB a.
ANOVAa ACTB
22.728 2 11.364 12.528 .000
86.173 95 .907
108.901 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = NFKB a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.5004 .2376 .114 -1.080 .079
-1.1477* .2295 .000 -1.707 -.588
.5004 .2376 .114 -.079 1.080
-.6474* .2424 .027 -1.238 -.057
1.1477* .2295 .000 .588 1.707
.6474* .2424 .027 .057 1.238
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = NFKB a.
337 NS1
Descriptivesa ACTB
33 1325841 1838114.4622 319974.7 674073.865 1977607.961 2720.6 7893452.3
22 3959.682 3978.7929 848.2815 2195.584 5723.780 146.1 13111.6
29 65040.694 100212.6060 18609.01 26921.857 103159.531 700.1 447502.8 84 544357.7 1306331.5918 142532.5 260866.324 827848.992 146.1 7893452.3 -/-
+/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = NS1 a.
ANOVAa ACTB
3E+013 2 1.662E+013 12.419 .000
1E+014 81 1.338E+012
1E+014 83
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = NS1 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
1321881.2* 318407.1 .000 543466.836 2100295.626 1260800.2* 294448.7 .000 540957.361 1980643.077 -1321881.2* 318407.1 .000 -2100295.626 -543466.836 -61081.012 327072.8 1.000 -860680.621 738518.596 -1260800.2* 294448.7 .000 -1980643.077 -540957.361 61081.0123 327072.8 1.000 -738518.596 860680.621 (J) genoty pe
+/- +/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = NS1 a.
338 TLR4
Descriptivesa ACTB
36 .280 .2791 .0465 .185 .374 .0 1.6
29 .558 .3325 .0618 .432 .685 .2 1.7
33 1.008 .8310 .1447 .713 1.303 .0 4.6
98 .607 .6189 .0625 .483 .731 .0 4.6
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = TLR4 a.
ANOVAa ACTB
9.234 2 4.617 15.710 .000
27.920 95 .294
37.154 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = TLR4 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.2787 .1353 .126 -.608 .051
-.7284* .1307 .000 -1.047 -.410
.2787 .1353 .126 -.051 .608
-.4497* .1380 .005 -.786 -.113
.7284* .1307 .000 .410 1.047
.4497* .1380 .005 .113 .786
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = TLR4 a.
339 TLR9
Descriptivesa ACTB
35 .541 .5834 .0986 .340 .741 .0 2.8
27 1.508 1.0388 .1999 1.097 1.919 .2 3.9
33 1.181 .8060 .1403 .896 1.467 .1 3.2
95 1.038 .8975 .0921 .855 1.221 .0 3.9
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = TLR9 a.
ANOVAa ACTB
15.298 2 7.649 11.648 .000
60.416 92 .657
75.714 94
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = TLR9 a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-.9672* .2076 .000 -1.473 -.461
-.6409* .1966 .005 -1.120 -.161
.9672* .2076 .000 .461 1.473
.3263 .2103 .372 -.186 .839
.6409* .1966 .005 .161 1.120
-.3263 .2103 .372 -.839 .186
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = TLR9 a.
340 TNF Alpha
Descriptivesa ACTB
36 1.409 1.8447 .3075 .784 2.033 .0 8.6
30 3.584 3.5722 .6522 2.250 4.918 .3 15.0
32 21.467 17.1666 3.0347 15.278 27.656 3.4 68.1
98 8.624 13.4468 1.3583 5.928 11.320 .0 68.1
-/- +/- +/+
Total
N Mean Std. Dev iat ion Std. Error Lower Bound Upper Bound 95% Conf idence Interv al f or
Mean
Minimum Maximum
gene = TNF Alpha a.
ANOVAa ACTB
7914.451 2 3957.226 39.060 .000
9624.668 95 101.312
17539.119 97
Between Groups Within Groups Total
Sum of
Squares df Mean Square F Sig.
gene = TNF Alpha a.
Post Hoc Tests
Multi ple Comparisonsa Dependent Variable: ACTB
Bonf erroni
-2.1757 2.4882 1.000 -8.240 3.888
-20.0585* 2.4455 .000 -26.018 -14.099
2.1757 2.4882 1.000 -3.888 8.240
-17.8828* 2.5579 .000 -24.117 -11.649
20.0585* 2.4455 .000 14.099 26.018
17.8828* 2.5579 .000 11.649 24.117
(J) genoty pe +/-
+/+
-/- +/+
-/- +/- (I) genot y pe -/-
+/-
+/+
Mean Dif f erence
(I-J) St d. Error Sig. Lower Bound Upper Bound 95% Conf idence Interv al
The mean dif f erence is signif icant at the . 05 lev el.
*.
gene = TNF Alpha a.