with an array of conserved cysteine residues

... lengths of 86 µm, 43 µm, 30 µm and rectangular patches with side lengths of 100 µm and 43 µm The individual metal patches are separated by a planar gap of 2 µm The thickness of the patches and the ... capacitance (Ceq) and inductance (Leq) given below.19 FIG 2 a) Three-dimensional drawing of a metamaterial-based terahertz absorber with a 4x4 array of patches with a size of 43 µm and two rectangular ... variation of the resonance characteristics of the structure as a function of (a) thickness of the Parylene layer, (b) gap height at the tip of the structure. Trang 6another resonance at 1.47 THz The array

Ngày tải lên: 19/11/2022, 11:45

... effect of these substrate analogues This is consistent with a simultaneous interaction of the 1,3BPGA analogues at both Ps and Pi phosphate-binding sites Comparison of the inhibition of T cruzi and ... design of novel classes of antitrypanosomatid drugs has become urgent Glycolysis plays an important role in all human-infective Trypanosomatidae and is, in some members of this family, the only ... complex of T brucei gGAPDH with an analogue of 1,3-BPGA, we chose to investigate the enzyme–inhibitor interactions by studying the kinetics of enzymatic inactivation with the native protein and with

Ngày tải lên: 20/02/2014, 02:21

... Trang 1and regulation by macrophages after stimulation withan immunomodulator of parasitic nematodes Ana Sofia Figueiredo1, Thomas Ho¨fer2, Christian Klotz3, Christine Sers4, Susanne Hartmann3, ... recombinant cystatin from Acanthocheilonema viteae; RSS, residual sum of squares; SP1, Sp1 transcription factor; STAT3, signal transducer and activator of transcription. Trang 2Nevertheless, many ... in many cell types A myr-iad of stimuli can activate these kinases, which, in turn, activate many other transcription factors and regulators of transcription, controlling the expression of many

Ngày tải lên: 29/03/2014, 23:20

... water equations with an improvement of wave energy dissipation Phung Dang Hieu 1,* , Phan Ngoc Vinh 2 1 Vietnam Institute of Seas and Islands, Hanoi, Vietnam 2 Institute of Mechanics, VAST, Vietnam ...    Trang 9Equation (37) can be solved numerically to obtain the values of qm j,k1 Then, the values of parameters B and v e are determined by Eqs (12) and (13) Values of R bx , R by and R bz ... diffusion of momentum due to the surface roller of wave breaking, the terms R bx , R by and R bz added to the right hand side of the momentum equations in the x, y, and z directions (Eqs (1), (2) and

Ngày tải lên: 23/07/2020, 01:58

... Mean and S.D (Standard Deviation) were calculated The statistical significance of the data was estimated by one-way analysis of variance and LSD test Mean comparisons were performed using HSD of ... physicochemical and biochemical analyses at 0, 3, 6 and 9 days of storage, and their quality and shelf life were evaluated by analysing the following parameters at 0 day and thereafter at regular interval of ... potential of radical scavenger and as a commercial source of poly-phenols (Bamdad et al., 2006) The clove oil has antimicrobial, antifungal, antiseptic, antiviral, aphrodisiac, and analeptic

Ngày tải lên: 23/09/2020, 00:36

... provide an excita-tion wavelength (k) of 494 nm, and an emission filter needs to pass an emission k of 517 nm Similarly, a 525 nm quantum dot nano-crystal, where emission k is a function of nanocrystal ... seamlessly within the stan-dard pathology workflow, capturing an important element of pathology-based mIHC imaging 2.3 Software for analyzing multiplexed images 2.3.1 Quantitation of chromogenic and ... through application of image analysis, with the use of landmark markers specifically to indicate tissue architecture Landmark markers can also accelerate scan times This workflow also can increase a

Ngày tải lên: 02/11/2022, 14:28

... Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues Jan T. Andersen 1 , Sune Justesen 2 , Burkhard ... Production of goat antisera Female goats were immunized subcutaneously with 50 lg of mutant shFcRn or mutant HC in 500 lL of NaCl ⁄ Pi (pH 7.3) and 750 lL of Freund’s complete adjuvant (Difco, ... Binding analyses were performed using ELISA analyses of anti-FcRn reactivity Wells of ELISA plates were coated with 100 lL of mutant shFcRn or hb2m (Abcam) at 2 lgÆmL)1, and blocked with

Ngày tải lên: 16/03/2014, 06:20

... in an appropriate volume of autoclaved aqua bidest and stored at -20 °C 3.1.5 Photometric determination of DNA and RNA concentration The concentration of DNA and RNA was measured with a Nanodrop ... separation of DNA fragments 27 3.1.4 Cleanup of DNA fragments 27 3.1.5 Photometric determination of DNA and RNA concentration 27 3.1.6 Isolation of RNA 27 3.1.7 Reverse transcription of RNA into ... arrays of epidermal growth factor (EGF)-like and Ca2+ binding EGF-like (cbEGF-like) domains, and one or two C-terminal type III transmembrane domains The WE domain is rich in tryptophan (W) and

Ngày tải lên: 19/11/2015, 16:33

... power and ability for self governance in the performance of control functions They are composed of a collection of hardware and software, which can perform the necessary control functions, without ... years, with the use of frequency domain methods and Laplace transforms in the 1930s and 1940s and the development of optimal control methods and state space analysis in the 1950s and 1960s Optimal ... developments in [1,2] Trang 2Controllers with a high degree of autonomy can be used in a variety of systems from manufacturing to unmanned space, atmospheric, ground, and underwater exploratory

Ngày tải lên: 26/01/2014, 07:20

... Overexpression and purification of the active-site domain of AcmA (A) SDS ⁄ 12.5% PAGE of cell extracts of 10 lL of E coli BL21(DE3) (pETAcmA) (lane 3) induced for h with IPTG Lane 2: 10 lL of purified ... masses (kDa) of standard proteins (lane M) are shown in the left margin Below the gel the lower part of lanes 5, and of the same gel is shown after week of renaturation The right half of the figure ... Expression of AcmA derivatives A1, A2, A3 and A4 in the L lactis NZ9000 mutants acmAD1 and acmAD1 DhtrA, visualized by zymographic analysis of culture supernatants of cells expressing the AcmA variants

Ngày tải lên: 19/02/2014, 18:20

... of Isoform B and against recombinant trypsin 4 By using these antibodies, we isolated and chemically identified trypsinogen 4 from extracts of both post mortem human brain and transi-ently transfected ... residue of the longer Isoform A isolated from the transiently transfected cells is methionine, as expec-ted, the N-terminal amino acid of Isoform B, isolated from human brain and transiently transfected ... that both Isoforms A and B of human trypsinogen 4 were expressed Cells trans-fected with the p72Mp28L(TTG) construct, however, expressed only the longer isoform (Isoform A) and no traces of the shorter

Ngày tải lên: 07/03/2014, 10:20

... valuation of an accident of type j happening to an oc- cupant of a passenger car ej: monetary valuation of an accident of type j happening to a truck wj: monetary valuation of an accident of type ... number of pedestrians and cyclists (N) and on a number of other factors (h) d,: risk that an accident of type j happens to a truck Trang 4bj: risk that an accident of type j happens to a pedestrian ... costs associated with the risk of death or injury to the occupants of the additional car Society Society Occupants of the additional car Their relatives and friends Society Trang 7TABLE l (continued)

Ngày tải lên: 07/03/2014, 11:20

... of these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme. To obtain an insight into how the many conserved residues ... permitted determination of pH-dependent activity of even the least active ChiB variants. We also conducted calculations of pK a values of several residues in ChiB variants with and without bound substrate, ... Union, grant no. BIO4-CT- 960670 and by the Norwegian Research Council, grant nos. 122004/112 and 140440/130. We thank Gustav Kolstad for helpful discussions and assistance with producing some of the

Ngày tải lên: 07/03/2014, 14:20

... Island, with ten thousand men, while an equal force should effect a junction with the army of Canada, by way of the Hudson This purpose he subsequently deferred for an advance into Pennsylvania,but ... receipt of price, by LEE AND SHEPARD, BOSTON [Illustration: GENL BURGOYNE.] Decisive Events in American History BURGOYNE'S INVASION OF 1777 WITH AN OUTLINE SKETCH OF THE AMERICAN INVASION OF CANADA, ... comparison And the quickness with which raw yeomanry wereformed into armies capable of fighting veteran troops, affords the strongest proof that the Americans are anation of soldiers So many specific

Ngày tải lên: 08/03/2014, 12:20

... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of bound sugars and the side-chains of three acidic residues in the conserved DXDXE ... in a similar manner to Tyr (Fig. 6A) [23]. In the catalytic mechanism of AD2, an oxazolinium ion intermediate could be formed with the assistance of an amino acid residue other than the DXDXE ... structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis Biochemistry... & van Aalten DM (2002) Structure of the D140N mutant

Ngày tải lên: 15/03/2014, 11:20

... lanes 1 and 6, 0; lanes 2 and 7, 5; lanes 3 and 8, 10; lanes 4 and 9, 20; lanes 5 and 10, 50 For proteinase K, the final concentrations were (lg ⁄ mL): lanes 1 and 6, 0; lanes 2 and 7, 0.5; lanes ... 0.5; lanes 3 and 8, 1.0; lanes 4 and 9, 5.0; lanes 5 and 10, 10 Lanes 1–5 and lanes 6–10 were analyzed by SDS ⁄ PAGE in the presence or absence of 2-mercaptoethanol, respectively. Trang 9the subsequent ... expression of TNSALP mutants in COS-1 cells. (A) COS-1 cells, which had been transfected with a plasmid enco-ding the wild-type (lanes 1 and 5), TNSALP(R433C) (lanes 2 and 6), TNSALP(R433H) (lanes 3 and

Ngày tải lên: 16/03/2014, 12:20

... roles of individual residues within the flexible second binding loop of human cystatin A in the inhibition of cysteine proteases Four recombinant variants of the inhibitor, each with a single mutation, ... rate constants The low dissociation rate constants of the complexes between the cystatin A mutants and papain were measured by displacement of the mutants from the complexes with an excess of a tighter-binding ... compatible with high-affinity interaction with papain The phi and psi angles of Gly75 deduced from the model are thus within the Ramachandran plot region sterically allowed for other types of residues

Ngày tải lên: 17/03/2014, 10:20

... presence or absence (lane 7) of different concentrations (lanes 1 and 4, 0.45 lg; lanes 2 and 5, 0.9 lg; lanes 3 and 6, 1.8 lg) of wild-type (lanes 1–3) and the E13E19 mutant (lanes 4–6) proteins ... A13 deletion mutant (lane 2), and the A19 mutant (lane 4) of p25 rum1 were examined as in Fig 1A,C (upper panels and lower panels, respectively). (B) Subsequent phosphorylation of GST fusion proteins ... experiment was quantified and expressed as a percentage of wild-type proteins as in Fig 2D (C) GST fusion proteins of wild-type (lane 1) and the A13A19 mutant (lane 2) of p25 rum1 were generated and examined

Ngày tải lên: 17/03/2014, 23:20

... understanding of the molecular determinants of the spectroscopic and electron transfer properties of this class of proteins Results and Discussion Structure description Overall, the structure of ... between molecules A and C involving main chain atoms of molecule A, namely oxygen of Asn60 and nitrogen of Gly63, and the side chains of Lys29 and Tyr39 of mol-ecule C Three of the four direct ... with larger discrepancies The first one is the single Gly63, and the second is a stretch of six residues from Gly70 to Ala75, with the largest difference of 1.68 A˚ between Gly70 of chains B and

Ngày tải lên: 23/03/2014, 05:22

... 2. The changes in the kinetic parameters of the D257A mutant were small relative to those of the D235A and D258A mutants (fourfold and twofold changes in k cat for oxidoreduc- tion and decarboxylation, ... arrangement of E234. The carboxy groups of E234 and D235 and the C1 carboxy and C2 hydroxy groups of l-malate are coplanarly chelated with Mn 2+ D258 and water are located axially above and beneath ... roles of conserved carboxylic acid residues in pigeon cytosolic NADP + -dependent malic enzyme Shuo-Chin Chang 1 *, Kuan-Yu Lin 1 *, Yu-Jung Chen 1 , Chin-Hung Lai 1 , Gu-Gang Chang 2 and Wei-Yuan

Ngày tải lên: 23/03/2014, 10:21