visualising pamp prr interactions using nanoscale imaging

... and reliable method for the detection of protein–protein interactions using yeast G-protein signaling In our system, protein–protein interactions were detected utilizing the knowledge that the ... protein–protein interactions Detection of interactions in the absence of FEBS Journal 276 (2009) 2636–2644 ª 2009 The Authors Journal compilation ª 2009 FEBS 2639 Detection system for protein–protein interactions ... suitable for yeast cell growth (30 °C) Several methods of detection of protein–protein interactions have been developed using G-protein signaling Medici et al established the Gpa1–Gas chimeric system...

Ngày tải lên: 16/03/2014, 01:20

... dealing with ‘writing’ patterns is however limited to using a single source Fig Bowtie-shaped ridge aperture In this work we describe using a nanoscale bowtie aperture array for parallel nanolithography ... writing using a × array of Bowtie apertures (a) SEM image of the mask, (b) AFM image of patterns produced in the photoresist Conclusions In conclusion, we describe a nanoscale lithography system using ... the metal film The roughness measured using atomic force microscopy (AFM) is less than nm over an area of 25 µm2 Apertures are defined in the metal film using FIB milling (FEI Nova 200 Dual Beam...

Ngày tải lên: 06/05/2014, 08:54

... and nanoscale light concentration These ridge apertures should have potentials in other 278 near-field applications such as ultrahigh density optical or magnetic data storage and nanoscale imaging ... of ridge apertures in both directions, which shows the advantage of using ridge apertures to concentrate light Nanolithography Using Ridge Apertures C-shaped ridge apertures are tested for nanolithography ... ridge aperture adopts the concept of ridge waveguide in microwave engineering [19] while having nanoscale dimensions and operating in the optical regime As shown in Figure 1, ridge apertures have...

Ngày tải lên: 06/05/2014, 08:55

... Rev Lett 95, 017402 (2005) L Wang, S M Uppuluri, E X Jin, and X Xu, “Nanolithography Using High Transmission Nanoscale Bowtie Apertures,” Nano lett 6, 361-364 (2006) E X Jin, and X Xu, “Enhanced ... are conducted using C and regular apertures fabricated in the aluminum film as the contact mask Only the C apertures are able to produce sub-60 nm features in photoresist, offering nanoscale optical ... important to control the exposure in order to obtain nanoscale holes Precise control of the exposure was realized by varying the exposure time using an electric shutter in millisecond precision...

Ngày tải lên: 06/05/2014, 08:55

... the intensity where the imaging contrast needs to be evaluated Figures and show how the imaging contrast is calculated and used for estimating lithography performance, using the bow-tie antenna ... intensity and imaging contrast (also called modulation) are two important factors for lithography patterning Photoresist is sensitive to the total field intensity, and sufficient imaging contrast ... this bow-tie aperture In this work, imaging contrasts are calculated at two radius, r = 25 nm and r = 50 nm at each distance, which is intended to find out if the imaging contrasts are sufficient...

Ngày tải lên: 06/05/2014, 08:58

... the combination of state-of-the-art imaging using aberrationcorrected electron microscopy with applied and computational mathematics will enable a “new age” of imaging in both the hard and soft ... conventional high-resolution TEM imaging and in atomic resolution Z-contrast imaging, the resolution of the final image is limited by the aberrations in the principal imaging lens For STEM, this means ... typical highdose imaging conditions described above Here, the imaging conditions are a beam current of 50 pA, a pixel dwell time of 20 s per pixel, and a pixel size of ˚2 0:05 A These imaging conditions...

Ngày tải lên: 30/05/2014, 00:33

... the left hemisphere using the Leica 1850 cryotome and were exposed to phosphor films overnight The films were read using the Cyclone Phosphor Imaging System and were analyzed using the Optiquant ... collected dynamically which were rebinned using a Fourier rebinning algorithm The images were reconstructed using a twodimensional Filter Back Projection using a Hanning Filter with a Nyquist cutoff ... was produced via MC-17 cyclotron using oxygen-18-enriched water Radioactivity was counted using a Capintec dose calibrator while low level counting was done using a well counter Inveon preclinical...

Ngày tải lên: 21/06/2014, 02:20

... CONTINUOUS SOLUTIONS USING THE FTM To obtain a model that can be implemented in the computer, the continuous initial-boundary-value problem has to be discretized Instead of using a direct discretization ... disadvantage of the FTM can be fixed by using a multirate approach for the simulation of the recursive systems DISCRETIZATION WITH A MULTIRATE APPROACH The basic idea using a multirate approach to the ... the external force fed (k) (compare Figure 2) Using the same path in the multirate system shown in Figure Multirate Simulations of String Vibrations Using the FTM would result in a long delay within...

Ngày tải lên: 23/06/2014, 01:20

... hypothesize that interactions between proteins evolved in a parsimonious way and that the set of correct domain-domain interactions is well approximated by the minimal set of domain interactions ... scoring (LP-score close to 1) interactions remains stable That is, interactions predicted under assumption of lower network reliability almost always are a subset of the interactions predicted under ... Since only a small number of interactions actually occur, out of all possible interactions between pairs of proteins containing domain pair {A, B}, detecting such specific interactions is difficult...

Ngày tải lên: 14/08/2014, 17:22

... early events following host-parasite interactions Therefore, the aim of this study was to investigate the pathogenic potential of Blastocystis, by studying the interactions of Blastocystis with ... et al 2004b) In a more recent study, correlation between the genotype and symptoms was evaluated using PCR subtyping and a significant correlation between subtype and the asymptomatic group was ... reports and experimental studies indicate that Blastocystis is not invasive but it is capable of causing gastrointestinal pathogenesis Experimentally, it was shown that germ free guinea pigs can...

Ngày tải lên: 11/09/2015, 09:11

... BONE DENSITOMETRY USING CT IMAGING LEI YANG B Eng A THESES SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING GRADUATE ... mechanisms will be introduced in section 2.2.2 To be able to measure bone mineral density accurately using CT imaging, certain artifacts and errors should be removed This is the main task of this work ... 1.1.4 Quantitative Computed Tomography Computed tomography imaging is an established technology for in vivo measurement of bone density In CT imaging, the two-dimensional internal structure of an...

Ngày tải lên: 02/10/2015, 12:55

... immobilized G-15 and S-15 DNA film in buffer using a Voigt-based viscoelastic model 78 Table 5-4 Analysis of the aptamer (G-15) and thrombin-aptamer complex in buffer using a Voigt-based viscoelastic model ... Summary Optical and acoustic biosensors have been successfully applied in the study of biomolecular interactions for several years In our study, surface plasmon resonance (SPR) spectroscopy, colorimetric ... this work would be invaluable in the study of DNA secondary structure and also the aptamer-protein interactions on surface, which in consequence will be useful in the development of DNA aptamer-based...

Ngày tải lên: 03/10/2015, 20:32

... D4, D2, serotonin type 2, norepinephrine, acetylcholine, and histamine [20-22] Functional brain imaging methods have been applied for studying schizophrenia These methods include positron emission ... [10], SPECT for studying CBF and neuroreceptors, and more recently, functional magnetic resonance imaging (fMRI) for measuring changes attributable to cerebral blood flow In this paper, after summarizing ... design and in reconstruction algorithms have substantially improved the quality of SPECT images using the Anger type gamma camera SPECT images can be merged with MRI and CT, creating a single...

Ngày tải lên: 02/11/2012, 11:12

... cloning process manually after using command to set up the server, and then you’ll learn how to start cloning on the clients C HA P TER USING UBUNTU SERVER FOR SYSTEM IMAGING Setting Up the Server ... easy, because the program will detect almost all settings automatically C HA P TER USING UBUNTU SERVER FOR SYSTEM IMAGING The first couple of questions ask you about the DNS configuration you want ... Once what you expected, and if it is, proceed with the configuration C HA P TER USING UBUNTU SERVER FOR SYSTEM IMAGING Listing 2-3 The DRBL Setup Program Shows What It Is Going to Configure Next...

Ngày tải lên: 19/10/2013, 02:20

... studies of the oligomerization of GPCRs and other protein–protein interactions [34–40] In these studies, BRET has been measured using Rluc and enhanced yellow fluorescent protein (EYFP) as luminescent ... described above AT1aD359-GFP2 was created by PCR using the angiotensin II receptor subtype 1a as template and subsequent subcloning into pGFP2-N3 using HindIII and BamHI as restriction enzymes ... spectra were recorded from 450 to 600 nm by exciting at 425 nm using the same conditions as above, where background was subtracted using nontransfected cells, and the spectra were normalized BRET...

Ngày tải lên: 08/03/2014, 09:20

... of a bowtie aperture on AFM probe with inset of idealized geometry Nanolithography using high transmission nanoscale ridge aperture probe aperture with a 180 × 180 nm outline dimension and a ... processes by combining probe lithography with nanoscale bowtie apertures It was shown that this combination was able to successfully manufacture nanoscale lines below the diffraction limit Lines ... smaller line width (150 nm) produced by the 300 nm aperture at high scan speed was a result of using a dose very near the threshold The 100 nm probe did not produce any lines 883 The depths of...

Ngày tải lên: 06/05/2014, 08:57

... performed on the largest subset of data to determine whether within assay variability would change when using the improved counting method We evaluated all of the tests performed on the lyophilized reference ... facilitates conservation of primary and secondary antibody stocks, which is an important factor when using http://www.virologyj.com/content/2/1/84 Figure complement separated by serum titers with Boxplots ... diluted 1:100 in Blotto, was incubated at 50 µl/well for h at room temperature Plaques were developed using 100 µl/well 3,3'diaminobenzidine HRP substrate (0.5 mg/ml DAB, 0.01% H2O2) prepared in DPBS...

Ngày tải lên: 19/06/2014, 08:20

... images using a fibre optic laser scanning confocal microscope (FOCM, Optiscan Pty Ltd, Melbourne, Australia) Imaging techniques Prior to image acquisition, the FOCM was calibrated by using focal ... 5(C1) Discussion Using a 3D imaging technique, this study investigates the 3D collagen structure in the superficial zone in relation to the physiological status of AC Since the 3D imaging technique ... collagen network in AC has not been resolved Using a specific dye for collagen (type I, II and III) and the fibre optic laser scanning confocal microscopy, a 3D imaging technique has been developed previously...

Ngày tải lên: 20/06/2014, 01:20