transplantation cell therapies and liver bioengineering

... and inflammation-associated cell surface molecules to elucidate the growth of, and metastatic tumor formation by, liver cancer Materials and methods Cell culture The human lung fibroblast cell ... inflammation- associated cell surface molecules by liver cancer cells The proportions of TLR1+ and TLR4+ cells were significantly higher in MHCC-LM3-(MRC-5)-CM cells than in controls, and were moderately ... greater in liver cancer cells cultured in MRC-5-CM than in liver cancer cells Trang 4cultured in normal medium (Fig 9) The proportion of cells expressing CD14 was lower in liver cancer cells cultured

Ngày tải lên: 14/01/2020, 23:21

... pro-cesses [27] 3.3 Cell 3.3.1 Source and Cell Type Cell types and their potential for new medical treatment are presented in Tables1and2 Stem cells represent a promising cell source due to their ... favors cell engraftment and angiogenesis 3.5 Where? Feasibility, safety, and cell retention are the com-mon features that may drive the choice of the cell delivery Different ways to inject the cells ... define the optimal type and the dose of stem cells, the route and timing of delivery after myocardial infarction, and long-term outcomes In addition, mechanisms of action and in parti-cular the

Ngày tải lên: 01/11/2022, 09:05

... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... (ESR1–3¢-UTR–WT) and pGL3m–ESR1–3¢-UTR–MUT (ESR1–3¢-UTR–MUT) in HEK293T and MCF-7 cells cotransfected with pcDNA-3.0–miR-22 or pcDNA-3.0 and synthetic miR-22 duplex or control RNA duplex,and in MDA-MB-231 cells ... ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and

Ngày tải lên: 22/03/2014, 21:20

... understand how and why cells rebel, we need to understand the normal functions of cell growth and reproduction. From the mid- nineteenth century on, research in cell biology, biochemistry, and molecular ... the cell to enter S phase and M phase. 6 Cell Biology and Cancer Figure 2. The cell cycle is an ordered process of events that occurs in four stages. During the two gap phases, G1 and G2, the cell ... cells to fill the gap. This characteristic is lost in cancer cells, which continue to grow after they touch other cells, causing a large mass of cells to form. • Normal cells age and die, and

Ngày tải lên: 29/03/2014, 01:20

... using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets Bioconjugate Chem 15, 87–98 1998 ´ S Andre et al ´ 53 Morris S, Ahmad N, Andre S, Kaltner ... lectin ligand cell surface binding was performed with the following human tumor lines: Croco II (B-lymphoblastoid cell line), CCRF-CEM (T-lymphoblastoid cell line), K-562 (erythroleukemia cell line), ... leukemia cell line), DU4475 (mammary carcinoma cell line) as well as C205, SW480 and SW620 (colon adenocarcinoma cell lines) Except for the Croco II line established in our laboratory [56] the cells

Ngày tải lên: 30/03/2014, 16:20

... sample, cells vary in size and shape, as well as in the dielectric and conductive properties of cellular components, such that the critical field and mem- brane relaxation time differ from cell to cell ... below the equilibrium value N, = Vcellcext (3 7- 40) Here N, is the number of molecules taken up by a single cell, Vcell is the cell volume, and text is the extracellular concentration in a large ... implications for medical therapies for electrical shock injury, and may also help us to understand the mechanism by which membranes recover 24 Cell Stress and Viability Complete cell viability, not

Ngày tải lên: 11/04/2014, 10:10

... attracting cytokines CCL22 and CXCL12 [22] These Treg cells can counter the effects of Th1 responses by cytotoxic T cells, Th1 cells, and NK cells In contrast, we found that LPS and IFN-g maturated ... 4, 8 and 24 hours in culture with LPS and IFN-g and were then assessed by flow cytometry, ELISA, and global gene and microRNA (miRNA) expression analysis Results: After 24 hours of LPS and IFN-g ... global changes of both gene and miR expres-sion associated with LPS and IFN-g induced DC matura-tion Gene and miR changes in DCs were assessed after 4, 8 and 24 hours of LPS and IFN-g stimulation To

Ngày tải lên: 18/06/2014, 16:20

... impact of beads and anti-CD3 on cells, we serially monitored for-ward scatter (a relative measure of cell size) in CD4 and CD8 cells after stimulation and restimulation CD4 and CD8 T cell size increased ... Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early days after stimulation, cells were CFSE-labeled and moni-tored ... exten-sive anti-CD3-T cell receptor crosslinking on a surface, and the costimulation provided by cell-cell interaction between T cells and Fc receptor positive accessory cells such as monocytes

Ngày tải lên: 18/06/2014, 16:20

... × 103 cells/μl, range 0.8-2.24; and 1.21 × 103 cells/μl, range 0.45-2.54, in the filgrastim and pegfilgrastim group, respectively; mono-cyte count = 0.25 × 103 cells/μl, range 0.05-0.35; and 0.23 ... circulation However, the relative distribution of CD4+ T cells, CD8+ T cells, CD19+ B cells and NK cells (defined as CD3-CD16 + CD56+ cells) within the lymphocyte population was unaffected by ... is a longer-acting form of G-CSF, whose effects on dendritic cell (DC) and regulatory T cell (Treg) mobilization, and on the in vivo and ex vivo release of immune modulating cytokines remain unexplored

Ngày tải lên: 18/06/2014, 16:20

... CD34+KG1a and Kasumi-1 cells incubated with/without DNR Results: Curcumin inhibited proliferation and induced apoptosis and G1/S arrest in both DNR-insensitive KG1a, Kasumi-1 and DNR-sensitive U937 cells ... CD34+KG1a and Kasumi-1cells were insensitive to DNR KG1a, Kasumi-1 and U937 AML cells were stained with PE-conjugated CD34 antibody and subjected to flow cytometry to determine the purity of CD34+ cells ... CD34+KG1a and Kasumi-1cells were insensitive to DNR (A) KG1a, Kasumi-1 and U937 cells were stained with PE-conjugated CD34 antibody and subjected to flow cytometry to determine the purity of CD34+cells

Ngày tải lên: 18/06/2014, 19:20

... oesophageal squamous cell carcinoma cell lines and an oesophageal adenocarci-noma cell line Moderate-differentiated cell lines OE19 (oesophageal adenocarcinoma cell line) and OE21 were obtained ... tumour tissues and oesophageal squamous cell carcinoma cancer cell lines, and is related to clinical pathology and prognosis of the patients with oesophageal squamous cell carcinoma Cellular function ... oesophageal squamous cell carcinoma We also provide new insights into the biological functions of MTSS1 and its role in oesophageal squamous cell carcinoma Material and methods Cell lines and human oesophageal

Ngày tải lên: 18/06/2014, 19:20

... endogenous p53 and p21 expression and decreased endogenous C-myc expression (Figure 4C) Thus, it can be inferred that the inhibition of cell proliferation and cell cycle arrest in CK2a knock-down cells ... p53, p21 and C-myc expression CK2a knockdown inhibits cell migration and invasion Migration and matrigel invasion assays were performed to examine the effect of CK2a on tumor cell migration and invasion, ... accordingly Flow cytometry assay Cells were harvested at an exponential growth phase, and single-cell suspensions containing 1 × 106 cells were fixed with 70% alcohol The cell cycle was monitored using

Ngày tải lên: 18/06/2014, 19:20

... González, Fernando Casafont and Manuel G Fleitas VI Contents Part Cell and Experimental Liver Transplantation 141 Chapter Cell Transplantation – A Possible Alternative to Orthotopic Liver Transplant ... the technical issues in living and deceased donor liver transplant procedures, cell and experimental liver transplantation, as well as the complications of liver transplantation Some of the very ... and Prospects of Living Donor Liver Transplantation in Egypt: 1268, Transplantation: 86 (2S) : 42930 Living Donor Liver Transplantation 37 Abdeldayem H (2010) Transparency and living donor liver...

Ngày tải lên: 27/06/2014, 13:20

... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell ... targets of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated ... required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins 331...

Ngày tải lên: 25/10/2013, 21:20

... Chang cells On the basis of the different usages of exon and exon 1a in Huh7 and Chang cells, we decided to analyze the relationship between usage of exon 1a and P1 promoter methylation In liver cell ... and OccDE9-overexpressing cells (Fig 6A) Specifically, in Chang and Huh7 cells, OccWT significantly decreased the expression of MMP2, MMP7, MMP9 and MMP14, and of MMP1, MMP2, MMP3, MMP7, MMP9 and ... fetal bovine serum for 24 h Experimental procedures Cell cultures and treatment Human liver cell lines Huh7, HepG2, Hep3B, and HLE, and Chang cells, were cultured in DMEM with 10% fetal bovine...

Ngày tải lên: 18/02/2014, 18:20

... on tissue Gb3 GSLs were extracted from kidney and liver of control and CsA-treated Fabry mice after weeks recovery post ERT, and from CsA-treated and untreated wildtype mice The Gb3 content was ... the Fabry liver VT1 binding detected Gb3 in the stellate Kupffer cells, distributed throughout the section, and in cells lining the portal triad The levels detected in Fabry mouse liver were ... Wild-type liver Wild-type liver Fabry liver A sphingomyelin B GSLs C GlcCer LacCer Gb3 Gb4 Gb5 GM3 GM2 GM1 10 Wild-type kidney 11 12 13 14 10 Fabry kidney 1 Fabry liver sphingomyelin E D Fabry liver...

Ngày tải lên: 19/02/2014, 07:20

... untreated control cells; lane 4, Glu-Pg-treated cells; lane 5, Glu-Pg- and mAb A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) ... control cells (B, C) Cells incubated with Glu-Pg: (B) residual adherent cells; (C) detached cytospun cells (D, E) Cells incubated with Glu-Pg and 0.5 lM of mAb A10.2: (D) residual adherent cells; ... cytospun cells (F) Cells incubated with Glu-Pg and 0.5 lM of mAb 34D3 No cells were recovered after cytospining the supernatant of control and Glu-Pg ⁄ mAb 34D3-treated cells *P < 0.0001 vs control cell- bound...

Ngày tải lên: 20/02/2014, 01:20

... Hepatitis and Liver Cancer: A National Strategy for Prevention and Control of Hepatitis B and C http://www.nap.edu/catalog/12793.html Hepatitis and Liver Cancer: A National Strategy for Prevention and ... Hepatitis and Liver Cancer: A National Strategy for Prevention and Control of Hepatitis B and C http://www.nap.edu/catalog/12793.html Hepatitis and Liver Cancer: A National Strategy for Prevention and ... role of state and local chronic disease surveillance in describing the burden of morbidity and mortality related to chronic hepatitis B and hepatitis C and related liver cirrhosis and cancer, the...

Ngày tải lên: 06/03/2014, 01:20

... reserved Hepatitis and Liver Cancer: A National Strategy for Prevention and Control of Hepatitis B and C http://www.nap.edu/catalog/12793.html  HEPATITIS AND LIVER CANCER Knowledge and Awareness ... of Hepatitis B and Hepatitis C Worldwide, 22 Prevalence and Incidence of Hepatitis B and Hepatitis C in the United States, 25 Hepatitis B, 25 Hepatitis C, 28  Liver Cancer and Liver Disease from ... Data Hepatitis and liver cancer : a national strategy for prevention and control of hepatitis B and C / Heather M Colvin and Abigail E Mitchell, editors ; Committee on the Prevention and Control...

Ngày tải lên: 06/03/2014, 01:20

... Unbound ligand was removed by washing the cells with NaCl ⁄ Pi, and the cells were incubated for h in the F-12 medium containing 10% fetal bovine serum Some cells on slides were fixed and mounted ... HSD) Fig Subcellular localization of 17b-HSD11 in CHO cells CHO cells stably expressing the myc-tagged 17b-HSD11at the C-terminus were fixed and stained with monoclonal antibody to myc and the fluorescein ... formation and distribution of 17b-HSD11 in CHO cells The cells were incubated with or without 200 lM oleic acid for 48 h, and stained with Oil Red O Distributions of 17b-HSD11 in parallel cells expressing...

Ngày tải lên: 07/03/2014, 05:20