traditional human embryonic stem cell culture

... model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells ... nervous system (Cavallaro et al., 2008; Cimadamore et al., 2011; Ferri et al., 2004; Puligilla et al., 2010) Therefore, it was expected that the knockdown of SOX2 in the ReN-VM neural stem cells ... embryonic stem cells (hESCs), and human neuronal development, using hESCs as a cellular model for neural differentiation 9.1.1 Identification of functional human lncRNAs Using a microarray approach,

Ngày tải lên: 09/09/2015, 17:55

... Trang 1A Human Embryonic Stem Cell–based Model of Trophoblast Formation LUO WENLONG National University of Singapore 2008 Trang 2 A Human Embryonic Stem Cell–based Model of Trophoblast ... A Human Embryonic Stem Cell–based Model of Trophoblast Formation (manuscript in preparation, 2009) 3 Inhibition of methylation releases the developmental plasticity of mouse embryonic stem cells ... Introductory statement Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the preimplantation embryo ESCs are pluripotent stem cells as, under well established culture conditions,

Ngày tải lên: 11/09/2015, 16:07

... the inner cell mass of blastocysts, embryonic stem cells (ESCs) are characterized by their ability for unlimited self-renewal and pluripotency ESCs are able to develop into almost all cell types ... developmental biology and regenerative medicine Human embryonic stem cells (hESCs) presumably preserve the molecular signatures of early development Cultured in suspension, hESCs spontaneously form ... downregulation of miR-200c miR-200c directly targeted the 3′-untranslated Abbreviations: hESCs, human embryonic stem cells; EB, embryoid body; TGF- β, transforming growth factor-β; FGF, fibroblast growth

Ngày tải lên: 02/11/2022, 14:37

... Trang 1Experimental Study of the Biological Properties of Human Embryonic Stem Cell–Derived Retinal Progenitor Cells Jingzhi Shao1, Peng-Yi Zhou1 & Guang-Hua Peng1,2 Retinal degenerative ... culture system that could harvest a substantial quantity of retinal progenitor cells (RPCs) from human embryonic stem cells (hESCs) within a relatively short period of time Furthermore, we transplanted ... progenitor cells (RPCs) The ESCs, which can self-renew and differentiate into any other type of cell, are the most promising sources of PPCs and RPCs It has been shown that embryonic stem cells (ESCs),

Ngày tải lên: 24/11/2022, 17:42

... cancer stem cells and embryonic stem cells (ESC) have similar cell surface markers [18,19] It has been hypothesized that the similarities shared by cancer stem cells and embryonic stem cells might ... posited to give rise to cancer stem cells; an alternative suggests that cancer stem cells arise from adult stem cells present in the tissues [2] These cancer stem cells, then, have been suggested ... regulated stem cell driven differentiation and cancer stem cell reprogramming that involves altered methylation in the service of great cell heterogeneity and plasticity Keywords: Human embryonic stem

Ngày tải lên: 17/06/2020, 16:55

... HPC; HPC-HBC, HPC-derived hepatoblast-like cell; HPC-HC, HPC-derived hepatocyte-like cell; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; MEF, mouse embryo fibroblast; FAF-BSA, ... functional hepatic cells for pharmaceutical research 2 Materials and methods 2.1 hPSCs culture A human embryonic stem cell (hESC) line H9 (WA09)[16,17]was obtained from WISC Bank (WiCell Research ... hepatocytes that were cultured for 48 h after plating the cells, as previously described[6,7] Cultures of primary human fetal hepatocytes, human normal fetal liver-CD34+ cells, and HepaRG® cells which

Ngày tải lên: 04/12/2022, 15:13

... and Systems Biology, Institute of Medical Science, University of Tokyo, Japan Introduction Mouse embryonic stem (ES) cells are established from the inner cell mass (ICM) of blastocysts ES cells ... Ptb) ⁄ ) embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo Furthermore, cell cycle ... alternative splicing is important for ES cell self-renewal and differentiation [3] However, the mechanisms by which molecules that Keywords cell cycle; embryonic stem cells; knockout mouse; polypyrimidine

Ngày tải lên: 07/03/2014, 00:20

... vascular cells differentiated from human ES cells Figure 1 Schematic representation of preparation of the transplanted vascular cells differentiated from human ES cells. human embryonic stem cells ... type 2 (VEGF-R2)-positive cells induced from mouse embryonic stem (ES) cells can differentiate into both endothelial cells (ECs) and mural cells (MCs) and these vascular cells construct blood vessel ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression

Ngày tải lên: 18/06/2014, 15:20

... undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved in hES cell function ... been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying ... of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture

Ngày tải lên: 18/06/2014, 15:20

... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells Trends Biotechnol 2004, ... 10 Cheng L, Hammond H, Ye Z, Zhan X, Dravid G Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture Stem Cells 2003, 21, 131-142 11 Cibelli JB, Stice SL, ... all cell types of the body and large culture Therefore, ES cells could be the choice of cells in stem cell therapy for various diseases One of the critical steps for stem cell therapy using ES cells

Ngày tải lên: 07/08/2014, 18:21

... source of cells to address this issue and eluci-date the exact pathways and intermediates between the embryonic stem cell and the mature adipocyte In parti-cular, mouse embryonic stem cells (mESCs) ... development and the embryonic origin of this lineage remain largely unknown Results: Here we performed genome-wide analysis of gene expression during adipogenesis of mouse embryonic stem cells (ESCs) ... distinct cellular intermediates between MSCs and mature adipocytes, such studies have been hampered by the lack of specific cell surface markers to identify and prospectively isolate these cells

Ngày tải lên: 09/08/2014, 20:22

... out the expression profile of the cells converged toward embryonic stem cells These data demonstrate these cells not only are a useful tool for the studies of cell differentiation both for the ... 3B) For co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure ... provide a valu-able source of cells for cell therapy studies in animal models of skeletal and neurological disorders Introduction Bone marrow derived human mesenchymal stem cells (hMSCs) are considered

Ngày tải lên: 10/08/2014, 05:21

... Open AccessDefining human mesenchymal stem cell Tracey L Bonfield1*, Mary T Nolan (Koloze)1, Donald P Lennon2, Arnold I Caplan2 Abstract Allogeneic human mesenchymal stem cells (hMSCs) can suppress ... confluency and the cells were then lifted from the dish with trypsin and replated at 4500 cells/cm2 to ensure they maintained active cell division As deter-mined by other studies, cells cultured using ... Introduction Human mesenchymal stem cells (hMSCs) from marrow residein situ as pericytes that are hypothesized to func-tion as sentinels to guard against self-surveillance by T-cells at sites

Ngày tải lên: 11/08/2014, 03:20

... in a specific cellular state is often referred to as the ‘epigenome’ [19] Abstract Human pluripotent cells such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) ... Prevalent in hESCs [66,67] ESC, embryonic stem cell; CTCF, CCCTC-binding factor, insulator associated protein; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; H2AZ, histone variant ... understanding of complex human diseases and their underlying genetic variants © 2010 BioMed Central Ltd Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into

Ngày tải lên: 11/08/2014, 12:21

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) are ... scientists to derive and successfully culture human embryonic stem cells (hES cells) from a human blastocyst, an early human embryo of approximately 200 cells, donated by a couple who had completed...

Ngày tải lên: 06/03/2014, 15:20

... base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole ... island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative ... epigenomics of cancer Cell 2007, 128:683-692 19 20 21 22 Hemberger M, Dean W, Reik W: Epigenetic dynamics of stem cells and cell lineage commitment: digging Waddington’s canal Nat Rev Mol Cell Biol 2009,...

Ngày tải lên: 09/08/2014, 23:20

... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...

Ngày tải lên: 14/08/2014, 08:20

... differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells (hESCs) as a paradigm for ... protein MEF   Mouse embryonic fibroblast xiv  Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... Feeder-free culture of human pluripotent stem cells H1 hESCs (passage number 20 to 35) and a human iPS cell line (passage number to 20) were grown feeder-free on Matrigel (BD Biosciences), cultured...

Ngày tải lên: 09/09/2015, 17:54

... important in human embryonic and neuronal developmental networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for ... pluripotency, neural stem cell identity, or neuronal differentiation RNA from the highly homogenous cell types derived from hESCs were hybridized onto two separate arrays: the custom-designed human lncRNA ... neuronal differentiation respectively   82  Chapter VI – Long Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency 6.1 Introduction LncRNAs are integral members of the ESC self-renewal...

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...

Ngày tải lên: 09/09/2015, 17:54