titration amplification and purification of hsv subheadings  3 1 3 4

... 159.43, 148.96, 148.92, 128.81, 122.00, 120.80, 120.69, 120.59, 120.53, 120.44, 111.36, 111.30, 111.03, 110.79, 69.65, 55.78 UV-Vis (DMF): λmax , nm (log ε) : 705 (4.85), 671 (4.82), 643 (4.54), ... and CHCl3:CH3OH (100:3) as the eluent Yield: 0.06 g (30%) IR (KBr tablet) ν max/cm−1: 3289 (N-H), 3070 (Ar-H), 2932–2834 (Aliph C-H), 1607, 1514, 1480, 1459, 1419, 1323, 1260, 1228, 1157, 1136, ... 120.14, 119.97, 117.69, 115.84, 115.45, 111.40, 111.07, 107.73, 71.43, 56.19 MS (ESI), (m/z): 317 [M + Na]+ 3.2.2 Metal-free phthalocyanine (4) A mixture of compound 3 (0.2 g, 0.68 mmol) and

Ngày tải lên: 12/01/2022, 23:33

... function of the operating conditions, these are often given as part of the specifications of the system.bar-1 Geometry, material, and dimensions of the hopper 2 Geometry, material, and dimensions of ... dimensions of the barrel 3 Dimensions, energy requirements, and configuration of heating/cooling arrangement 4 Diameter and material of the screw 5 Shape, height, thickness, and pitch of screw flights ... detailed information and understanding of thermal processes and systems The synthesis of information from a variety of sources plays an important part in the development of an acceptable design

Ngày tải lên: 06/08/2014, 15:20

... the same as shown in Figure 3.3 The links in Figure 3.2 and 3.3 are defined based on Definition 2.11 (event order at the physical system layer) Figure 3.2: Two Cases of Event Execution at the Physical ... Figure 3.3: Normalized Event Parallelism at the Physical System Layer prob norm Π = (2+2+2+4+2+1+2) / 7 = 2.14 Service Center 1 Service Center 2 Service Center 3 Service Center 4 D prob = 10 minutes ... a parallelism of 3,167 events per second Due to the overheads at the simulator layer and the limitation in the number of PPs, the simulator can only exploit a parallelism of 121 events per second

Ngày tải lên: 16/09/2015, 17:12

... be improper under the law and most moral theories 6 Omega, Inc., a publicly held corporation, has assets of $100 million and annual earnings in the range of $13 to $15 million Omega owns three ... a myriad of legal and moral implications From a profitability standpoint, especially in the case of a company on the brink of economic failure, ignoring the requirements and whims of customers ... ANALYSIS OF THE VARIOUS OPTIONS: How does each of the options impact the following goals of Pharmakon? 1) Productivity 2) Fiscal soundness 3) Minority presence and preservation of minority gains 4)

Ngày tải lên: 08/11/2019, 14:53

... Virginia, 129 U.S 114, 9 S.Ct 231, 32 L.Ed 623 (1889) b See, for example, Pharmaceutical Manufacturers’ Association v New Mexico Board of Pharmacy, 86 N.M 571, 525 P.2d 931 (N.M App 1974); State ... v Attorney Registration and Disciplinary Commission, 496 U.S 91, 110 S.Ct 2281, 110 L.Ed.2d 83 (1990)] 2-2 Commerce Clause In 1957, Rhodes and several other Georgia landowners entered into ... Case 2.1 379 U.S 241, 85 S.Ct 348, 13 L.Ed.2d 258, 1 Empl Prac Dec P 9712 Supreme Court of the United States HEART OF ATLANTA MOTEL, INC., Appellant, Mr Justice CLARK delivered the opinion of

Ngày tải lên: 08/11/2019, 14:54

... Virginia, 129 U.S 114, 9 S.Ct 231, 32 L.Ed 623 (1889) b See, for example, Pharmaceutical Manufacturers’ Association v New Mexico Board of Pharmacy, 86 N.M 571, 525 P.2d 931 (N.M App 1974); State ... v Attorney Registration and Disciplinary Commission, 496 U.S 91, 110 S.Ct 2281, 110 L.Ed.2d 83 (1990)] 2-2 Commerce Clause In 1957, Rhodes and several other Georgia landowners entered into ... Case 2.1 379 U.S 241, 85 S.Ct 348, 13 L.Ed.2d 258, 1 Empl Prac Dec P 9712 Supreme Court of the United States HEART OF ATLANTA MOTEL, INC., Appellant, Mr Justice CLARK delivered the opinion of

Ngày tải lên: 31/01/2020, 14:15

... TS 1 2 3 4 5 6 7 8 9 10 SM/AntiA-ScFv kDa 116 66 45 35 25 18 14 kDa 116 66 45 35 25 18 14 AntiA-ScFv 1 M 2 3 4 5 6 M 1 2 3 SM/antiA-ScFv antiA-ScFv kDa 70 55 45 35 25 15 90 110 160 ... by ELISA sample Positive control- from company 0,013 0,338 ± 0,018 0,048 ± 0,016 0,686 ± 0,033 0,839 ± 0,001 Note: TN1- TN4 4 replicates of each sample, 2 replicates for control TB average ... Academia Journal of Biology, 42(2): 65–74 https://doi.org/10.15625/2615-9023/v42n2.13864 *Corresponding author email: lethuhong@ibt.ac.vn ©2020 Vietnam Academy of Science and Technology (VAST)

Ngày tải lên: 24/07/2020, 02:42

... on the economic value of being on target and  reducing variation and dispelling the  traditional view of conformance to  specifications:  Loss Trang 21of CommerceTrang 23A Systems Perspective 4 Measurement, ... place No programs Trang 32 Quality system standards adopted by  International Organization for  Standardization in 1987; revised in 1994 and 2000  Technical specifications and criteria to be used as rules, guidelines, or definitions of characteristics to ensure that materials, products, processes, and services are fit for their purpose ... Technical specifications and criteria to be used as rules, guidelines, or definitions of characteristics to ensure that materials, products, processes, and services are fit for their purpose Trang 33expectations and requirements.Trang 34 Provide confidence to internal management and other employees that 

Ngày tải lên: 04/11/2020, 23:33

... (0-300) 1 (0-257) 135 (5-300) Absence 19 20 (0-200) 0.75 (0-10) 120 (16-270) 50 (0-180) 1 (0-257) 140 (10-300) Presence 42 12.5 (0-300) 0.75 (0-100) 90 (0-285) 95 (205-300) 10(0-207) 145 (5-300) ... number of metastases 94 R = 0.3458, p = 0.0006 R = 0.3333, p = 0.0010 NS R = 0.2945, p = 0.0040 R = -0.2212, p = 0.0331 NS Trang 8(R = 0.4303, p = 0.0198 for SOCS-3, Figure 7C and R =0.8254, p ... ΙΙI/IV 63 20 (0-300) 0.75(0-100) 80 (0-285) 60 (0-300) 1 (0-257) 120 (0-300) Absence 30 30 (0-300) 0 (0-45) 20 (0-270) 20 (0-160) 44.5 (0-244) 90 (0-250) Presence 64 17.5 (0-300) 0.5 (0-100) 95

Ngày tải lên: 05/11/2020, 01:28

... 61,2 , , , , , , , , , , 60,5 61,4 60,7 61,3 60,9 61,2 61,1 61,3 60,9 61,4 60,7 61,2 60,3 61,1 61,0 61,5 61,3 61,9 61,4 61,3 61,6 61,0 61,7 61,1 60,9 61,5 61,6 61,4 61,5 61,2 61,6 61,3 61,8 61,1 ... 61,2 61,4 60,2 61,2 61,3 60,4 61,4 60,8 61,2 60,6 61,6 60,2 61,3 60,3 60,7 60,9 61,2 60,5 61,0 61,4 61,1 60,9 61,5 61,4 60,6 61,2 60,1 61,3 61,1 61,3 60,3 61,3 60,6 61,7 60,6 61,2 60,8 61,3 61,0 ... 61,1 61,7 60,9 62,2 61,1 62,1 61,0 61,5 61,7 62,3 62,3 61,7 62,9 62,5 62,8 62,6 61,5 62,1 62,6 61,6 62,5 62,4 62,3 62,1 62,3 62,2 62,1 Thiết kế thí nghiệm &Xử lý số liệu MS: CH3309 03.10.2017

Ngày tải lên: 17/07/2021, 08:40

... CH2-triazole), 2.45 (s, 3H) 13C NMR (100 MHz, CDCl3) δ 173.1 (C=N), 171.2, 168.6 (C=N), 142.0, 141.9, 135.9, 134.7, 129.6, 129.5, 129.2, 128.3, 127.4, 127.3, 123.1, 95.1 (C-SO2) , 45.2 (CH2-triazole), 44.8 ... concentration of 1× 10 −3M) 5b Cl 2.5× 10 −4 b 39.4± 4.2 5c Br 4.3× 10 −4 b 26.3± 3.6 5e F 4.8× 10 −4 b 20.5± 0.6 5f I 1.7× 10 −4 b 41.9± 4.1 5g MeO 2.6× 10 −4 b 26.5± 1.9 5h MeS 1.7× 10 −4 b 33.3± 1.7 ... CH2-triazole), 3.80 (t, J = 12.0 Hz, 1H, CH2-triazole) 13C NMR (100 MHz, CDCl3) δ 174.6 (C=N), 168.9 (C=N), 136.8, 135.6, 134.8, 133.1, 130.3, 130.0, 129.8, 128.8, 128.2, 128.0, Trang 10127.0, 125.7,

Ngày tải lên: 12/01/2022, 23:17

... with 354-amino acid and a molecular mass of 43–47 kDa.1,3 The crystal structure of human serum PON1 has not been determined yet, but it is available for recombinant PON1 variant (G1A5, G1C4, G3C6, ... fraction of cell lysate of IPTG induced 2 h BL21(DE3) pET-SUMO-hPON1 Lane 2: Soluble fraction of cell lysate of IPTG induced 3 h BL21(DE3) pET-SUMO-hPON1 Lane 3: Soluble fraction of cell lysate of ... induced 4 h BL21(DE3) pET-SUMO-hPON1 Lane 4: Soluble fraction of cell lysate of IPTG induced 6 h BL21(DE3) pET-SUMO-hPON1 Lane 5: Soluble fraction of cell lysate of IPTG induced 8 h BL21(DE3) pET-SUMO-hPON1

Ngày tải lên: 12/01/2022, 23:41

... Biol 1992;8:429–462 [PubMed: 1335745] 3 Johnson DA, Akamine P, Radzio-Andzelm E, Madhusudan M, Taylor SS Dynamics of cAMP-dependent protein kinase Chem Rev 2001;101:2243–2270 [PubMed: 11749372] ... ESI-TOF: PKI5–24 calculated 2279.4 m/z, found 2278.6 m/z ; PLN1–20 calculated 2440.8 m/z, found 2440.1 m/z; R9C-PLN1–20 calculated 2387.9 m/z, found 2387.1 m/z; R14del-PLN1–20 calculated 2284.4 ... deleting arginine 14, results in lethal, hereditary cardiomyopathy Proc Natl Acad Sci 2006;103:1388–1393 [PubMed: 16432188] 11 Opella SJ, Ma C, Marassi FM Nuclear magnetic resonance of membrane-associated

Ngày tải lên: 30/07/2022, 21:44

... (minutes) 160 120 80 40 0 35 30 25 20 15 10 time (minutes) 1040 720 480 242 1040 720 480 242 P1 P2 P3 60 40 20 0 30 20 10 0 20 15 10 5 0 30 25 20 15 10 Diameter (nm) P1 P2 P3 25 20 15 10 5 0 30 25 ... 0 0.1 0.2 23.5 nm 0.1 0.2 0.3 19 nm 0.9 0.7 0.4 14.5 nm SEC #2 - CC SEC #1 - CC SEC #1 - All Size 0 0 0.1 28.0 nm 0 0.1 0.2 23.5 nm 0.1 0.2 0.3 19 nm 0.9 0.7 0.4 14.5 nm SEC #2 - CC SEC #1 - ... homogeneity of the smallest 14.5 nm sized NLP through iterative SEC of the elution fraction that is to the right of the peak’s center 14.5 19.0 23.5 28.0 A. B. 50x10-3 40 30 20 10 0 58 56 54 52 50 48

Ngày tải lên: 01/11/2022, 09:06

... 1 LITERATURE REVIEW 1 1.1 Giant liver fluke Fasciola gigantica 1 1.1.1 Classification 1 1.1.2 Morphology 1 1.1.3 Life cycle 2 1.1.4 Fascioliasis epidemiology 3 1.1.5 Symptoms and signs 4 1.1.6 ... research 121.6.1 International research 12 1.6.2 National research 14 CHAPTER 2 CONTENT AND METHODS 15 2.1 Place of administration 15 2.2 Contents 15 2.3 Methods 15 2.3.1 Rabbit immunization 15 ... 15 2.3.2 Ouchterlony assay 16 2.3.3 Purification of IgG antibodies from immunized rabbit 16 2.3.4 SDS-PAGE 17 2.3.5 Indirect ELISA 18 CHAPTER 3 RESULTS AND DISCUSSION 20 3.1 Detection of specific

Ngày tải lên: 17/11/2022, 17:52

... B 30 20 10 Time (h) 16 24 48 16 24 48 n 16 24 48 ODN (2 µg) 30 2C4 15 10 E Lip ODN cont Curc Contr F Dead cells (%) C Dead c cells (%) 0.5 30 15 10 n e 0.5 ODN (µg) 80 60 40 20 10 Cont (µg) 10 ... al (20 03) Targeted inhibition of Stat3 with a 2 5 14 19 20 21 22 23 24 25 26 27 28 29 30 decoy oligonucleotide abrogates head and neck cancer cell growth Proc Natl Acad Sci USA 10 0, 4 13 8 – 41 43 Barton ... (ARC, grant 31 33) and RFBR 06- 04- 4 919 6 References Benekli M, Baer MR, Baumann H & Wetzier M (20 03) Signal transducer and activator of transcription proteins in leukemias Blood 10 1, 2 940 –29 54 Zhong...

Ngày tải lên: 18/02/2014, 08:20

... Dev 17 , 10 09 10 18 34 Szutorisz H, Canzonetta C, Georgiou A, Chow CM, Tora L & Dillon N (2005) Formation of an active Globin locus activation during differentiation 35 36 37 38 39 40 41 42 43 44 ... 11 , 5 13 525 30 Cook PR (19 99) The organization of replication and transcription Science 2 84, 17 90 17 95 31 Riggs AD (19 90) DNA methylation and late replication probably aid cell memory, and type ... 7, 13 9 3 14 01 Elefanty AG, Robb L, Birner R & Begley CG (19 97) Hematopoietic-specific genes are not induced during in vitro differentiation of scl-null embryonic stem cells Blood 90, 1 43 5 14 47 ...

Ngày tải lên: 07/03/2014, 12:20

... process and as a goal Human Relations 46 : 17 5 19 2 Ison, R.L (19 93) Changing community attitudes Rangeland Journal 15 : 15 4 16 6 Jiggins, J (19 95) Problem led R&D In Grassland into the 21st Century ... planting 19 61 19 97 Floods, decreasing rainfall, population growth, improved hygiene, new diseases, high incidence, better health care 19 19 Kimiri (smallpox), forced labor 19 41 Plague 19 43 Famine 19 49 ... Foot -and- mouth disease; Exotic trees and crops 19 52 Emergency state, forced into villages 19 57 Land demarcation 19 61 Floods 19 64 Famine, yellow maize 19 70 New ticks species 19 73 New varieties of...

Ngày tải lên: 18/06/2014, 19:20

... 1, 2, 3) Factor Xa cleavage of GP1-MBP 28 60 40 30 20 GP1 17 14 GP2 B 98 62 49 B GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV GP1pMAL-c2x:GP1 Figure gami cells transformed with ... t4 Pa t3 N H S Pa t1 LASV IgG Assay NPbac B 3. 5 1/ 100 3. 0 1/ 200 2.5 O.D Pa t2 0.0 2.0 1. 5 1. 0 0.5 Pa t9 Pa t1 Pa t1 Pa t8 Pa t7 Pa t6 Pa t5 Pa t4 Pa t3 Patient # LASV IgG Assay GP2bac C 3. 5 1/ 100 ... Vaccines 20 04, 3( 2) :1 03- 11 1 McCormick JB: Clinical, epidemiologic, and therapeutic aspects of Lassa fever Med Microbiol Immunol 19 86, 17 5 :1 53- 15 5 McCormick JB: Epidemiology and control of Lassa...

Ngày tải lên: 20/06/2014, 01:20

... 38 8280 38 8887 38 9520 39 019 2 39 13 0 0 39 1 933 V-599 38 41 1 9 38 45 84 38 48 26 38 5 233 38 68 91 38 6990 38 70 23 38 712 4 38 712 9 38 7 13 0 38 7 13 1 38 71 73 38 844 6 39 17 13 3 917 71 39 2 43 8 39 30 84 39 319 8 39 32 73 3 932 82 39 349 2 ... AY 744 680 L 1 14 27 L 116 19 AY 744 678 DQ4 016 90 AY 744 6 84 L 11 43 2 AF 317 645 M 9 31 30 AB 111 085 AF 147 457 AY67 637 0 AY67 636 8 AY67 635 9 AF 533 079 AY 13 5 41 9 AY 145 715 AY 145 716 AY 338 4 93 AY 145 730 AY 145 726 AY 145 718 AY 145 7 23 ... AY496872 AB 111 080 L 1 14 24 AY09 933 6 AY09 933 7 AB 111 0 81 AY702 032 AY 038 605 AY 146 772 AY 146 765 AY 146 767 AY 146 776 AY702 030 AY702 033 DQ3 41 2 09 DQ3 41 2 08 DQ3 41 2 02 DQ3 712 45 DQ177899 DQ177900 DQ367720 DQ177898...

Ngày tải lên: 20/06/2014, 01:20