... wildtype enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268 The oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and ... bonds The interactions should maintain the structure of the C-terminal region In the wild-type enzyme, no bonds were observed between the N-terminal region and the C-terminal region on the molecular ... ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments...
Ngày tải lên: 21/02/2014, 03:20
THE BUSINESS CAREER IN ITS PUBLIC RELATIONS potx
... the individual works in the collection are in the public domain in the United States If an individual work is in the public domain in the United States and you are located in the United States, ... value in his business—as in all else in life—of the standard thing, the genuine thing, the thing that will bear the test as contrasted with the shoddy, or the inferior, or the spurious Our technological ... far-reaching in its influence and most helpful in raising business character Thoughts such as these prompted the recent establishing of the lectureship on "The Morals of Trade" in connection with the...
Ngày tải lên: 05/03/2014, 16:20
The Elizabethan Parish in its Ecclesiastical and Financial Aspects ppt
... laid down in the injunctions and articles they were sworn to observe, was the keeping in repair of the church fabric and its appurtenances, as well as the procuring and the maintaining in good ... they CHAPTER I 12 shall certify in Stratford bowe Chappell bothe of the vew making by the workmen, of the gathering of the rate already made, of their making a new rate and of the gathering thereof; ... after the conduct of their minister, so the minister was required to fill the office of a censor upon the behavior of the wardens and to report to the ordinary their delinquencies as, indeed, the...
Ngày tải lên: 24/03/2014, 02:20
Dynamic Planet Mercury in the Context of Its Environment docx
... constraints on the interior structure by determining the moments of inertia of the principal axes If a liquid layer, such as an outer core, decouples the inner core from the crust, then the moment ... showing the mission timeline, with extensive use of the gravitational assist technique developed for Mariner 10 during the five year cruise, and the first orbiting of Mercury during the nominal ... the uncertainty in , while Cm/C is determined to an accuracy that is principally limited by the uncertainties in o and If the ratio Cm/C is equal to 1, then the core of Mercury is solid If the...
Ngày tải lên: 06/03/2014, 19:20
Báo cáo lâm nghiệp: "Evaluation of changes in the landscape management and its influence on animal migration in the vicinity of the D1 motorway in Central Bohemia" ppsx
... anthropogenic pressure influencing the landscape structures in the vicinity of the linear construction in the form of a motorway, and especially due to the accompanying structures of linear or polygon ... and having service functions The individual images were fixed into a system of coordinates A line set on the layer modified in this manner designates the centre of the motorway within the investigated ... created that takes in 200 m on each side from the centre of the motorway and which stipulates the extent of the polygon in the area of interest In the polygon thus marked out, the individual biotopes...
Ngày tải lên: 07/08/2014, 10:21
Environmental regulation and economic competitiveness: Evidence from the textile industry in Vietnam
... technical aspect in the dyeing-finishing process It is the liquid ratio in dyeing The quantity of chemicals in the machine is determined by the concentration of the dyeing batch and most of these chemicals ... or the ‘envelope’ The inspectors received the money and turned a blind eye to the pollution as they are making a profit from the polluting operation They are then much concerned with maintaining ... guard-net In the oil heating room Oil spilt on the floor Namdinh Silk Co Outside the dyeing unit WIN TEX VIEW co Oil heater In the dyeing room Spinning room Yenmy Dyeing Co and Phonoi Industrial...
Ngày tải lên: 24/08/2013, 18:48
Tài liệu A REVIEW OF INDOOR AIR POLLUTION AND HEALTH PROBLEMS FROM THE VIEWPOINT OF ENVIRONMENTAL HYGIENE: FOCUSING ON THE STUDIES OF INDOOR AIR ENVIRONMENT IN JAPAN COMPARED TO THOSE OF FOREIGN COUNTRIES pptx
... stuffiness.39) The effect of building materials regulating indoor humidity on the indoor environment was studied to identify the in uence on the psycho-physiologic condition of the occupants.40) ... predominantly in indoor air samples.93) The dominant path of phthalates intake was the ingestion of foodstuffs compared to inhalation of indoor air by children.94) n-Tetradecane is an alkane with the ... combustion gases in residences and buildings CO is well-known to cause poisoning by CO-hemoglobin (Hb) formation, inhibiting oxygen utilization by internal organs NO2 sources in buildings include gas...
Ngày tải lên: 17/02/2014, 22:20
Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... transcription of the DNASE1 messages containing exons or 1a To examine promoter activity in the 5¢-flanking region of exon in DNASE1, we first obtained the )1386M construct by introducing the )1386 to ... related proteins, as shown in Fig 1B To evaluate whether the Sp1-binding site in the DNASE1 distal promoter is crucial for expression, a mutated binding site was introduced into the )197H construct, ... alternative promoters in DNASE1 results in no generation of protein isoforms The 5¢-UTR of eukaryotic mRNA in uences the initiation step of protein synthesis and thereby in part determines the translational...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx
... comprising Ôresidues in the protruding domain consisting of the type VI b-turn and the first part of b-strand 3Õ (i.e the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop ... MDG1 (as determined by Yang et al [16]) and its main secondary features (B) Main synthetic peptides used in the study and their minimal inhibitory concentration (MIC in lM) for the growth of M ... peptides containing the substituted peptide B (Table 2, L–K, N–M and P–Q), indicating that the properties of the loop drive the properties of larger peptides enclosing the loop Binding capacity...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
... Enzyme kinetic study The Km (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence ... determined as described in (A) in the presence of increasing concentrations of Mg2+ (C) pH dependency of the enzyme was examined as described above using several buffers with increasing pH values The ... hydrolysis, in the presence of increasing concentrations of NaF The PPase activated PPi hydrolysis rate was then calculated Larval molting inhibition assay To confirm whether the native PPase enzyme is involved...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: "The Software Architecture for the First Challenge on Generating Instructions in Virtual Environments" docx
... server instance It then sends the game world to the client and the server instance and disconnects from them, ready to handle new connections from other clients The client and the server instance ... possible, in order to maximize the number of users willing to play the game Unsurprisingly, we had to spend the majority of the programming time on the 3D graphics (based on the free jMonkeyEngine ... considerably In the future, we will extend the GIVE Challenge to harder tasks Possibilities includ making GIVE into a dialogue challenge by allowing the user to speak as well as act in the world; running...
Ngày tải lên: 22/02/2014, 02:20
Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt
... conclusion, these results strongly suggest that Pc binding accelerates the cGMP-binding-initiated conformational change in Pabc Together with the result showing that Pc inhibits cGMP-binding to Pabc ... [3H]cGMP binding to Pabc by interrupting the entry of [3H]cGMP into a noncatalytic site in Pabc This indicates that the scheme in which Pc binds to Pabc first and then cGMP binds to the Pabcc ... homogenates are incubated with GTPcS, the Pab content in membranes is increased 20–30% by binding of the Pab ⁄ Pc complex existing in the soluble fraction [13] Therefore, binding of the Pab ⁄ Pc...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains ... transmembrane domains in VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells Identification ... apoptosis in the bystander-uninfected population, given the fact that VBARP is abundantly present in many of the tested human primary cells Understanding the functions and identifying the other regulatory...
Ngày tải lên: 07/03/2014, 21:20
Risks Ahead for the Financial Industry in a Changing Interest Rate Environment pdf
... THE FINANCIAL INDUSTRY IN A CHANGING INTEREST RATE ENVIRONMENT short-term rates At the same time, increased reliance on wholesale funding (instead of deposits with low and relatively stable interest ... Regarding the wealth effect, spending and investment decisions may be sensitive to changes in net wealth incurred by changes in interest rates Both effects would then feed back into the financial ... potentially adding to maturity mismatches Effects of changes in the interest rate structure on financial institutions differ The effects of changes in the interest structure differ across financial institutions...
Ngày tải lên: 15/03/2014, 01:20
Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain pptx
... to see whether common structural features exist in the N-termini of GAF-domain-containing PDEs that might contribute to intramolecular signalling in a similar manner Another point merits discussion ... regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity The role of the N-terminus of the PDE11A4 GAF tandem domain The N-termini that precede the ... that the N-terminus profoundly affects GAF signalling [27] We therefore probed whether the 196residue N-terminus which precedes the hPDE11A4 1644 GAF tandem affects intramolecular signalling The...
Ngày tải lên: 16/03/2014, 06:20